Loopamp® A型インフルエンザウイルス検出試薬キット
Contents
1. RNA dR 1 Ri 8 strip tubes 6 DNA BST DNA pol ee 12U RT 1 5U 5 3 dATP tte 17 27g 5 3 dCTP 16 4 g 5 3 dGTP 17 8zg 5 3 dTTP 16 9zg eo 24 1 7g ee 0 39zg2. A 98 89 83 84 100 0 40 40 97 69 82 84 97 59 39 40 A 84 4 27 32 100 0 17 17 71 99 23 32
3. 2 FluA PC FLUA 4 FluA PC FLUA 5 6
4. FluA PC FLUA NC gt 1 2 100 A A
5. RNase DNase ii 7 28C 7 70C 2 8 72 RN
6. 3 gt 4 s 2 3 lt 4 4 RNA dRMR 1 RNA dRMR 2 RNA dRMR 2 2 8 C 3 RNA dRMR
7. B 3 Streptococcus pneumoniae Staphylococcus aureus Haemophilus influenzae Moraxella catarrhalis Klebsiella pneumoniae Escherichia coli Legionella pneumophila Pseudomonas aeruginosa Mycoplasma pneumoniae 2 Chlamydia pneumoniae Herpes simplex virus 1 Adenovirus Rhinovirus Respiratory Syncytial Virus Parainfluenza virus 3 Human metapneumovirus HIuman coronaVirus 3 A H1 12 H2 1 H3 5 H4 1 H5 6 H6 1 H7 3 H8 1 H9 1 H10 1 H11 1 H12 1 2 3 4 5 6 H13 1 H14 1 H15 1 A H1 12 1 1 Loopamp A H1N1 pdm
8. FluA PM FLUA 62 5C A cDNA cD LAMP RNA A DNA LAMP 1 1 2 F
9. RNA RNA EDTA 80C 5 95C 2 3 4 14 A FluA PC FLUA NC 1 2 B
10. 3 NO 10 xL lt gt LL Loopamp 10 gt RNA GRMR 5 gt ni 15xL iL 10zL LAMP FLUA
11. 20zL 0 5 10gL 10 100L 8 LAMP 600 700nm 62 5C 0 5 E 240 260nm 350 370nm 2 RNA Loopamp 10 QIAamp Viral RNA Mini Kit RNA Mini Ki 3 RNA 1 5 2
12. 4 2 8 C 5 3 1 2 PP 3 PET rE 2
13. 1 3 4 4 18 2mg dL H 2 E RNA E 20 1mg dL 1 460 4 95mg dL 100 1mg mL 1mg mL 1mg mL 1mg mL
14. HPLC 4 FluA PC FLUA A RNA cDNA EKN FluA EKN FLUA ED PC ELUA PC FIUA EKN FLUA MN 0 NC A RNA A LAMP Loop mediated Isothermal Amplification LAMP 2 6
15. Nt 2 ei 1 5 FluA PC FLUA NC 5z g mL B
16. 4 A RNA A 20 A FluA PM FLUA RNA Loopamp RNA Rn GRMR D A 3
17. A A
18. KW 380073 B 22200AMX00936000 2013 2 2 2010 F 10 1 Loopamp A 2 A A A A A
19. 25L NC FluA PC 5 gt 62 5C 35 80 5 95 2 a 1 2 06 06 THE 015 0 OA em 03
20. 1mg mL 0 01mg mL 0 1mg mL 1mg mL 0 35mg mL 2 g mL 0 5 g mL 4 FluA PC FLUA 10 zL 5 2 Me 6 7 5 8 8 2 A 1 2 ai 62 5C 20 3
21. 2 RNA 2 3 4 RNA QIAamp Viral dRMR 2 8C R Ei A dRMR 22 2 8 5 E NC FluA PM FLUA FluA PC FLUA 1 4 RNA 2 15 7L FluA PM FLUA GRMR 10gL 25 L
22. MAA 98 8 83 84 100 09 40 40 97 6 82 84 97 5 39 40 A hs 84 27 32 100 0 17 17 71 9 23 32 94 1 16 17 H im 4 a
23. 94 19 16 17 0 3 gt 4 DU Ef Ke 9 6 7 A 5 A 5 5 A 6 Loopamp 380073 B 7 QIAamp Viral RNA Mini Kit RNA 124 84 40
24. 08 02 GGSUNSNSENHG20 RSC 38 02 1 0 0 0 gt gt 0 5 10 15 20 25 30 935 0 5 10 15 g0 25 3 35 1 2 gt 1 2 3 4 5 6 2 7 8 9 10 11 12 13 Loopamp LA 320C B 1 0 5 62 5 2 62 5C 35 3 35 80C 5 95 C 2 LAMP
25. DNA RNA RNA RNNase RNase DNase RNase DNase RNA
26. MA 1 2 2 5 0 3 00 4 MO E 0 RNA RNA 1 1 2 FluA PM FLUA FluA PC FLUA NC
27. 8 6 Loopamp A 48 LMP463 1 Notomi T et al Nucleic Acids Research 28 No 12 e63 2000 2 Nagamine K et al Clin Chem 47 No 9 1742 1743 2001 3 Mori Y et al Biochem Biophys Res Commun 289 No 1 150 154 2001 67 715 1999 0120 308 421 Tomita N et al Nat Protoc 3 No 5 877 882 2008 54 No 3 6 329 O114 143 amp gkm 143
28. 40 0 2 5 86 79 13 15 71 49 5 7 3 1 3 lt 7 39 41 4 4 95 1 100 0 11 12 4 4 3 3 91 7 100 0 100 0 52 53 7 8 3 3 TR 98 1 87 5 100 0 53 53 8 8 3 3 EN 5 100 0 100 0 100 0 nasal swab 2 nasal swab 3 LAMP A B 3 7 5 7 26 29 2 2 1 2 A 71 4 89 7 100 0 50 0 Ul ec 7 7 28 29 2 2 2 2 throat swab 2 00 0 96 6 100 0 100 0 7 7 29 29 2 2 2 2 HI je 1000 1000 1000 1000 throat swab 2 throat swab 3 LAMP 1 0 1 250 2 10
29. 1 57 g FUMUPM PLUAO eee eeees 0 72mLX 1 1 A FIP FluAFIP 483 12ng A S 3 1 FluAF3 1 29 17 ng A F3 2 FluAF3 2 29 30ng A BIP FluABIP 478 60 ng A B3 1 FluAB3 1 28 80ng A B3 2 FluAB3 2 28 89ng A LoopF FluAFL 96 76ng A LoopB FluABL 99 42ng FluA PC ELUAOD ooo 0 16 mLX 1 NC ee 0 16mLx 1 1 Bacillus stearothermophilus DNA Polymerase I 5 exonuclease DNA 2 Avian Myeloblastosis Virus 3 A In uenzaA 283 H3N2
30. A dRMR FluA PC FLUA F uA PC FLUA FluA PC FLUA Loopamp
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