SYBR Green Realtime PCR Master Mix -Plus
Contents
1. rk 3 oo I o o EDI lt SA EZ Z Ezi EZ EH mum El Et E EET E Ed r El TE ERES E ICIO EO 5202255555 sa a EB ie 2505950007 m ET ET EH EJ EJ FE so zi ETT Sa ELE BIE E ES ET EST ES LES 2522250255 OH 55 EZ EZ Ez EZ EZ EZ EZ EH R uu mimi a goemoomooono M CJ OJ mM L3 r3 72 C x 40 000 data collection PERO 0 00 06000000000 600 0 H O O 0 Melting Curve 95 C 6010 95 C 151 60 C 150 72 C 300 0 0 000000000000000000000 00000000000000 0000000000000000 Fa EHH C3 E3 c3 Om pod os c3 c Hg 4 ET r 00000000000000000 DODODO 5 0000000000000 0000000000 2 mJ El L1 EZ mJ L1 E 1 L1 L1 L1 E El 21 tc m gt 02 L1 000 000000000 0 0 000 0 0 0000000 2 00000000 0 0 0 00000000 000000000000 00000000000000 0 000000000 L1 L1 L1 CI mJ E mJ E El mJ 22 mJ El L1 E N 0000000000 0000000000 L1 E L1 E L1 El mJ 1 L1 El ESTE a a L32. e s pes noo oo 00205400 o E SES ri amp a al 2 2 225555253250 225555252550 mOoOmOo Oooo ET rz EH 1 EE FE ono oo gaoa s a E nooo Lt Slds ET C3 ET E1 E1 E1 O 22 21 22 EX me a ff eam a 2 1 5 ET ET E3 EJ E1 EZ EZ EJ EJ o EE 222222522250 H SE E EEB EE EZ EZ EZ EZ Se ET ET EZ E3 EJ og a lo og og L3 o o C4 L3 El O mn o og L3 og o EH zu CJ E zm o dmo O NOO 0000000 00 Code No 1st 0 0000 eDNAL 0 D 00 ReverTra Ace a 0 H 0 O 0 dNTPs 1001111 FOKZIQI 00 00 0 10 000 1 101 ReverTra Ace 50 000U x 1 TRT 102 RNase Inhibitor Native type 2 500Ux 1 SIN 101 2 500 50 SIN 102 RNase Inhibitor Recombinant type 2 500Ux 1 SIN 201 2 500Ux 50 SIN 202 Realtime PCR Master Mix imLx1 QPK 101T 1mL x 5 QPK 101 SYBR Green Realtime PCR Master Mix imLx1 QPK 201T 1mL x 5 QPK 201 0000000 0000000 00 00 LineGene D 0 0 DODO 00 A 12 mn lm nn 00000000 UIUlDMOOO O OOODOOOUO 0000 00000 MOOD 530 8230 1 1000000000 20 80 TEL 06 634
3. Molecular Probes Inc ABI PRISM The Perkin Elmer Corporation 0000000 UU UU Oj ooo 00 4 000 000 000 SYBR Green 0 L3 r3 NG 00 ut CRO O O ET r n L3 L3 L3 DODOOOOOOOOOOOODOOOOOOOPCRODODOO C YBR Green Realtime PCR Master Mix Plus 5 L pol z El LIO L L3 ag oooooo AAO EE EO MIME nm T3 LI m Er IB EE C E 2 2 22 2 L3 ET E1 i yo aao 32000000 aa aera a a E E E EIE p E pz rc a 5500 nl EE E E EE nm B 2 nr B 3 a 3 B ram Hi Em S rer FOOD DODOS E ENa 7 0d 7 o agag aaa Bm FE ET ao Ez d FT EH ISA S E Ep Cee Om r3 r3 5 2050030 Io 2 2232 Em ES Ed EI 22 220223 Se ae E E E r ET E1EH 0 00 0 00000000000
4. lon a collect LO rc 2 m O EEE o E oOzoOaoO CT OJ o Oooo CT OJ B Oooo J OJ mq ma m5 m mad lun L1 00000 00000 Temperature Transition Rate 0 O 202 1 000000000 0000000 PCRQ Temperature Transition Rate 2 C sec 0000000000000000 00000000000 OU OOOO y ete 000 000 000 000 0000000 LOJ 0200000000 data collection PCRIUDOO S900ODOOOOOO 5500 72 C 95 C 30 4 95 C 5 x40 OOO 55 C 10 72 C 151 data collection 4 O O 0 Melting Curve H L 2 2 ML 5 HL 0 4 UL 04 H L SYBR Green Realtime PCR Master Mix Plus 000 001200000000 2 HL 10 O O BioFlux 0000000000 0 0 0 0 00 e d ub PCRO D MR D o4uMIDODOOO 10410 SYBR Green Realtime PCR Master Mix Plus 000 0000 a 25 mop am 050 r3 o yoo IOJ ou ISO CT o o CC F H CT oj IOJ o Lumoj E oo Cl m O O o omoj 2 za Cl m O A omoj ou DOJ Elmi J ce E Ei o mO a ada ES oH mo oo ug SOO mO oo OOOO Os o C3 o Et O SOGO Ed n6 70 aao oo ag und 22023 oo a EEE yo 6 12 ots Sel rae Elo Exc n Elm EKE CENE L3 C3 c SO ma Doe OSO oo E 2 am TELE n 0 5 4 noe aoao go ommommamm oo
5. L3 L3 CT DODOPCRIDOOOOOOOOOOODOODOOOOO 0 00 0 0 DNA 1st strand cDNA D E B E LO E 000 cDNA 16 uL 25 uL 2 uL 2 uL 5 H L 50 u L O ABI PRISM 7700 00 00000000000000 000000 PCRO 0 O 0100000 044 00 00 50410 UHO ODUUO OUOU SYBR Green Realtime PCR Master Mix Plus LU 0000 nm ENE Do Gm B Mm EE Si Eli Ek Elb selin EE Po mm B MA oy mm 50 02 ao Oy od SE Kiril ny ouj DJ yodo oom LO oo 52 mm mO 2300 Ey oa E Sooo FT r 30 2o 9 m El 30 omj mmm m c E mim o o onta aa o aa o 2 mO ao E y po E Oooo Ug OJ J Oj OO E1 L3 o os o 222223 oo some E LO o O CJ aa iii o EE pie good mm a DAG o 2 og OJ O OO OJ HTH Do Om SO 0006 os Bas E ao BS 2113 mo 22322322 2552522555 o El SA FOS os oO 2320952 F oo oo mna Hae EEE Go agga SSS Sere 660520560588 Ez EZ ET aa a e Ed ara a IEI DQ 1 iH a eo Li 221 227 E G EA EE OOOoOGm GroOs Og EJ 2223222022 Kana EST Ge fp ct ft e EJ EEE oE in EE E ET Ez Ezi EZ Z a ee SSeS aQ ZE E ES o o Se o 0200000000000 2000 0 0 0 O data colection 00000000000 000000000 PCRO 0 0 0 0116000000000 60100 72 95 C 60
6. y 95 C 150 x400 000 60 C 150 data collection 72 C 450 PCRO O 1 22000000000000 60 95 C 60 4 95 C 150 x40 000 60 C 6011 data collection 5 6 H L 10 HL 1 2 uL 1 2 uL 2 HL 20 HL SYBR Green Realtime PCR Master Mix Plus 000 001200000000 Roche LightCycler 0 0 0 00 000000000090 000000 PCRO D 0 0100000 064 0 00 0 0 2001 SYBR Green Realtime PCR Master Mix Plus 000 0000 00000 000000 000000000 000000000 5 nd mj ooga mad coco o0 I o0 Soco o0 ooga pom coasa pep EE Ez E ET 4 OP F1 EH Ec ooooo E EE EE EYE ET E1 E ET E3 EJ EH F3 EJ E ET EZ E1 EH EJ E ET EJ 7 E3 EJ E ET EZ EJ EJ EJ E ET EZ EJ EH EJ E ET EZ EJ EJ E ET EJ EJ EJ E ET E1 EJ 0 KOO L3 L3 g C3 L3 L3 g C3 L3 L3 g C3 mo oo L3 C3 L3 ro Fa Ed F3 Fa B3 r4 41 EH mp Ka L3 r3 rr L3 r3 L3 r3 rr L3 r3 L3 r3 rr 3 r3 nooo L3 r3 r3 LI p Eb ELE ET pr m pm p o Ear rara cc mu yolumla ima ege me COmneoomoomoomopooOo ana een wees el L ea L ngA 2020500 of ooo LIO Oo Uo Ooo 2 Ooo Hos Oo
7. 0000 DODODO 000000 00 00000000000 0 0000000000 TON Ido ION L3 C3 L3 5 EET E i E ESL ES FE HOOD oao EE o PO DODOCDO ES Ea Ey ABA Dmoooooo TON Co 000000000000 s SYBR Green Realtime PCR Master Mix Plus 00000 PERO 2000000000 0000 0000 dNTP MgCl ll Taq DNA 000 O O Tagi O 00000 SYBR Green 0000000000 000000000 000000000000 OOJ OOJ OOJ OOJ OOJ SOO SOO LI E E SOO SOO SOO SOO SOO SOO 21 EIE oa L3 L3 L3 L3 L2 L3 L3 L3 L3 L3 oa o 2011 30mer GCO 400 60760 00000000000 Oooo L3 ET F3 Oooo L3 ET F3 L3 F3 L3 E3 ET F3 F3 L3 p E1 3 ag EJ 00000000 00000000 00000000 FT E3 E3 ET o L3 E3 E3 3 EJ L3 E3 E3 E 3 EJ EIE tt ea O PCR Lu BI PRISM 77001 0000000000000 0000000 0000000 00 0 0 LineGene 0000000000 00000 uuu 0 Oist strand cDN 101 00000000 0 cDNA 0 LI LI LI U D 0 0000000000000 1st strand cDNA O O 0O O00 ReverTra Ace a 2 Code FSK 101 LI LI LI 00000 dT D D 0 231 L3 3 E
8. 06 08 SYBR Green Realtime PCR Master Mix Plus Code No QPK 211 211T 00000 TOYOBO CO LTD Life Science Department OSAKA JAPAN 4 A ALE 0000000000000000000000000000 0 2 0000000000000000000000000000 0 3 0000000000000000000000000000 0 9 0000000000000000000000000000 0 4 00000000000000000000000000 0 00 9 OO ABI PRISM 77001 0000000000000000 0 6 Roche LightCycler Q 0000000000000000 7 O BioFlux LineGeneQ O 0000000000000000 9 5 09 000000000000 0000000000 10 00000000000000000000000000000 12 0 000 1 EEHAJ dHUEBA EJE HU B 1 BTE ULUHHUHUHHHHHHHEHHEHHEHEHEHEHLHLULU AMETE 1 6 5 1 EEE F Hoffmann La Roche Ltd Roche Molecular Systems Inc The Perkin Elmer Corporation Purchase of this product is accompanied by a limited license to use it in the Polymerase Chain Reaction PCR process for The Research Field in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up front license fee either by payment to Perkin Elmer or as purchased i e an authorized thermal cycler LightCycler fi Idaho Technology Inc Roche Molecular Systems Inc SYBR
9. 8 3786 FAX 06 6348 3833 E mail order_lifescience bio toyobo co jp 0000 00000 0 0 MOOD l 103 8530 0 00000000000 170 90 TEL 03 3660 4819 FAX 03 3660 4951 E mail order_lifescience bio toyobo co jp U UUMOOOODOODOOOOUO 00000 000 TEL 06 6348 3888 FAX 06 6348 3833 00 0 0 9 000 12 00 13 009 17 00 TI 00 00 00 00 E mail techosk bio toyobo co jp URL http www toyobo co jp bio
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