SYBR Green Realtime PCR Master Mix
Contents
1. 1 4 E 5 77001 0000000000000 0000000 Lineen 0 00000 00000 00000 000 cDNA oo go O ooo ogg ogg amA ogH goon oooO gt lt raoga a o a g O gp 2 Og O g 2 16 uL 25 uL 2 2 5 HL ABI PRISM 77000 00 00 0000 00 00 0000 9000000 0 010 0000 0 45 501 L2. ABI PRISM 7700 0 42 C 20 95 C 50 95 C 150 60 C 150 x400 0 0 0 data collection Roche LightCycler 0 42 C 20 72 C 450 x400 000 1 95 C 50 1 95 50 729 150 data collectiom 55 100 BioFlux 42 20 1 95 50 1 95 150 400 000 data collection 60 C 150 72 C 300 1 00000000000 a E E 5 5 5 E gt 0 2 1 00000000000 5
3. 33 g 200000000 0000000 O 1 anga 20000000 S ooo H o Ya 72 x400000 data collection 0 000 000 000 0 6 0000 Melting 95 600 95 150 609 150 72 C 300 1 RT PCR Nase Inhibitor Code SIN 101 102 0 5 2 Inhibitor ReverTra 0 RTO 0 L 0 0 30 1
4. 550 00 72 95 300 95 C 50 400000 55 100 72 C 150 data collection 1 0000 Melting 221 5 uL O0 00 0 0 00 00 0 0 0 0 0 0 00 0000 0 45 SYBR Green Realtime Master o2 20 ga o2 2o ooa oa 20 oa 20 aae o 5 3 o2 o0 ZH ac Haoa og 25529 oo oo g 200952 9 92 2 2 ogo 23 5 9
5. o of goog Kong 362 coo 00 oO oogo20 o aago 8 e oocoogoog HOHA 8 SALG 9 9 584 S
6. 1 12 2 02 00 02 00 1 19 O 163 03 oag z9 o o go m A Tr 05 5 9 gja A o ogo g g 5 o 3293932 3733484 g H S S A E 2 15 H S 0 9 2 2 9 lt lt J
7. Green Realtime Master 0000 7 62 o o Og O goao qoooogoaoa SG o oo AG og 1 og 805 o 7 n oo og H i O 5 2o05 g oo 5 g 10 O g Dogo o o gt lt oO 116 ooo oo oloo D gt oo 1 9 5 5 O vonga lt 6 o5 2 ogo 2 10
8. o 2 o goa Oo o 75200 3 g o o o o O a O S m og o o 22 00000000 9 0000 00 0000000000 0 SYBR Green Realtime Master 00000SYBR Green0 0000000 ao ao aA o ao aA ao ao aA ao aA a aA ao ao A o ao A oa ona oa o oa o oa o 200 30 GCO 400 6051
9. RTO O O O RNase Inhibitor L 32 uL ReverTra 1000 L 1 HRL 0000 33 uL 0 44 50y L 000 1 Green Realtime PCR Master 25 uL 00000 1 10u 2 uL 00000 2 10u 2 uL RTO O O ReverTra 3U u L 5 uL SuL 0000 50 uL SYBR Green Realtime Master 0000 0 120 60000000600 000 0 0 0 01 00000 0 201 LO LineGeneU 00000 10 ao a o 0 1 215 9
10. oo 565 5 a 5 00 m gt 0200000000000 20 00 00 0 data colection 00000000000 18000 00 000 0 6 7270 95 600 1 95 150 x400 000 60 C 150 data collection 72 C 450 609 95 C 600 1 95 150 x400 000 60 C 60 data collection 64 uL 10 0 8 uL 0 8 uL 2 uL 20 uL 0 0 Roche Lightcycler o 0000000000000000 90000060 0100000 0 45 20 L SYBR Green Realtime Master 0000 ogogo Hp Oo o g 3 mnm g 6 5 ogo 5 a0 oa g 850 o o
11. S e 2 o Jogo a 5 a Ig gojo a 0000000 530 8230 20 80 TEL 06 6348 3786 FAX 06 6348 3833 E mail 0000 0000000 103 8530 00 0000 00000 170 90 TEL 03 3660 4819 03 3660 4951 E mail 0000 0000 000 0000 00000 000 TEL 06 6348 3888 06 6348 3833 0000 9 000 12 00 13 000 17 00 00000 00 00 E mail techosk bio toyobo co jp http www toyobo co jp seihin xr lifescience html
12. SYBR Green Realtime PCR Master MIX Code 201 201 CO Science Department OSAKA JAPAN 8 20 28 00000000000000000000000000000 3 3 65 PRISM 77000 0000000000000000 0 5 Roche 7 BioFlux 00 0000000000000 9 0 10000 10 12 13 O AOO UO Hoffmann La Roche Roche Molecular Systems Inc The Perkin Elmer Corporation Purchase of this product is accompanied by a limited license to use it in the Polymerase Chain Reaction PCR process for The Research Field in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up front license fee either by payment to Perkin El
13. mer or as purchased i e an authorized thermal cycler LightCycler Idaho Technology Inc Roche Molecular Systems Inc Molecular Probes Inc ABI PRISM The Perkin Elmer Corporation Green Probes Inc ooog oooooogo HA SS gH
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