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1. 121 C 20 19 6 1 2 32. 1 000ppm 1 29 1 121 C 20 2 0 05 3 2 10 C 1
3. NAG 1 R 1 2 10 C 4 2 R 2 2 10 C 4 0 1mL 0 1mL 1 5mL 1 5mL 37 C 83 0 5mL 0 5mL 37 C 5
4. 2012 4 11 20600AMZ01216000 2011 1 10 N D 38549000 N L NAG B Type 1 2 3 4 5 0 059 6 R
5. R 1 72549114 479111498 15mLx4 R 2 72549212 479111504 10mLx2 1 8 N NAG 15 1116 1989 2 8 N NAG 61 6 1402 1988 3 N D 32 11 1222 1988 4 6 methy 2 pyridy N acety 1 thio 8 D glucosaminide MPT NAG N acety 8 D glucosaminidase NAG 15 5 839 1992 5 MPT NAG NAG 7150 15 5 845 1992 6 6 methy 2 pyridy N acety 1 thio 8 D glucosaminide MPT NAG NAG JJCLA 20 2 1
6. 1 340nm AEs min AEb min AEs min AEb min x 2 1 NAG 1U L X 1 06 8875 x 0 1 1 MPT 340nm L mo cm 11 5IU NAG 5 61U g n 201 L NAG B S1005249 11
7. 1 1 AE min Z 0 020 2 NAG 501U L AE min 0 025 0 040 2 340nm 376 700nm 2 1 2 69 3 NAG 10IU L 20 C V 5 09 4 0 8 300IU L NAG x y y 1 003x 0 192 r 1 000 50 Tn 1 1 2
8. K 4 5 NAG 6 7 8 2 10 C 9 1
9. 200mM R 1 pH4 50 6 2 N 1 8 D R 2 7 4mg mL N 6 2 N 1 D MPT NAG MPT NAG N D NAG 6 2 MPT MPT 340nm NAG CHeOH CHs CHeOH N NAG OH CHs yO rmo gt ET rts HO HO NHCOCHs NHCOCHs MPT NAG MPT NAG 2 10 1 20 C 1 pH 8 0 4 0 NAG
10. 34 1995 7 30 155 156 1993 8 15 37 2001 TEL 03 4582 5420 FAX 03 3238 4590 1 L NAG B S1005249 11
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