pBApo-CMV Neo DNA
Contents
1. DNA v201103 Ys Tel 077 543 6116 bx 07784319792. Adenovirus Dual Expression Kit 6170 0 10mM Tris HCI pH8 0 1mM EDTA OR 4 553 bp 1 dideoxy 2 IRE BamHI EcoRI Hind Ill 1 APA Clai EcoRV 2 TaKaRa pBApo CMV Neo DNA EcoRV Clal SV40 polyA CMV IE promoter BamHl Xbal Sse8387 Hind Ill HSV TK polyA NeoR pBApo CMV Neo SV40 promoter pBApo CMV Neo DNA 3240 aot S _ i
3. 2 Lot No pBApo CMV Neo DNA CMV IE promoter A ORF microRNA ORF A vitro vivo
4. 53 bp Purity 1 Confirmed to maintain the promoter region and Cloning Sites by dideoxy sequencing method Note 2 Shown to be cleaved ata single site by BamH EcoR and Hind Ill and his product is for research use only It is not intended for use in therapeutic or diagnostic procedures for humans or animals Also do not use this product as food cosmetic or household item etc Takara products may not be resold or transferred modified for resale or transfer or used to manufacture commercial products without written approval from TAKARA BIO INC If you require licenses for other use please contact us by phone at 81 77 543 7247 or from our website at www takara bio com Users must contact TAKARA when they plan to use this product for purposes other than research use The viral supernatants produced by adenovirus vector could contain potentially hazardous recombinant virus depending on an insert gene Due caution must be exercised in this production and handling of recombinant adenovirus Please follow the guideline for experi ments using recombinant DNA issued by the relevant authorities and the safety committee of your organization or your country in using this product Takara is not liable for any accident or damage caused by the use of this product at two sites by C a I and EcoRV v201103 pBApo CMV Neo DNA Code No 3240 Size 20 ug Shipping at 20 C Store at 20 C
5. pBApo CMV Neo DNA Code No 3240 Size 20 ug Shipping at 20 C Store at 20 C 2 years from date of receipt under proper storage conditions Lot No Concentration ug ul Volume ul Vector map for pBApo CMV Neo DNA Regarding protocol please refer to the product manual supplied with this vector EcoRV al EcoRI SV40 polyA CMV IE promoter EF BamH Description Xbal pBApo CMV Neo DNA is a simple expression vector for mammalian cells NeoR This vector has a promoter from Cytomegalovirus CMV IE promoter PolyA signal from Thymidine Kinase of Herpes Simple Virus and neomycin resistant gene as a selection marker By inserting the ORF of target gene into MCS the expression plasmid for the target gene can be constructed This vector can be also used to express the transcript of pri microRNA In addition it is easy to transfer the cassette of promoter ORF PolyA signal from this vector to adenovirus vector especially to pAxcwit SV40 promoter supplied in Adenovirus Dual Expression Kit Cat 6170 Since adenovirus vector has high infection efficiency and wide target cell spectrum it is suitable for in vitroand in vivo gene transduction Sse8387 Hind III HSV TK polyA EcoRV Cal pBApo CMV Neo Form 10mM Tris HCl pH8 0 1mM EDTA Preparation Purified by ion exchange column Neomycin resistant gene as a selection marker of pBApo CMV Neo DNA Cat 3240 Chain length 4 5
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