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フィーダー細胞（SL10）

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1. 5 iPS SL10 DMEM high glucose 10 FBS 1mM Sodium Pyruvate Penlsilin Streptomycin ReproCoat 1 30 ReproCoat 1 37 C C0 2 15mL 4 C SL10 8 mL 3 SL10 37C 2 15mL 1mL 4 170g 1 000 rpm 4 5 5 1 5 x 10 cells mL SL10
2. 6 1 ReproCoat 2 7 CO0 37C C0 8 37C CO0 4 1 ReproCoat 24 wel 200300 gL 12 well 500 LL 2 1 5x10 cells mL RCHEMD001 Rrimate ES Cell Meddium RCHEMD003 004 RCHEFC003 Feeder Cells MEF http wwwrreprocell com E mal info repro reprocell com WWW reDrocell com
3. ReproCELL SL10 Cat RCHEFCO01 80C 6 ES iPS ES C PS Takahashi K et al Ce 131 861 72 2007 iPS 6x10 cells 60mm

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