DS-W取説 - 株式会社リーゾ
Contents
1. 2 3 mm DNA 1 OOO 1 40OO DNA 4 5 7O0 DNA F013F0 12. DNA PCR Template DNA 20 ng 1XBuffer dNTPs 02 mM primer 05 uM Taq polymerase QO 6U Total 15 ul Stratagene Paq5OOO PCR 95C 2 min 94C 30 sec 55C 30 sec 35 cycles 72C 60 sec 72C 7 min 10 DNA F013F0 W A 2 9 2 B201 029 852 9351 FAX O20 4623 561 1 E mail info rizo co jp http www rizo co jp Copyright e201 1 RIZO Inc All Right Reserved
3. S 15 4 TE PCR DNA F013F0 W DNA DNA
4. 20 25C 6 1 15C 3O0 40 DNA F013F0 W 1 1 v v 70 TE 10 mM Tris HCL 1 mM EDTA pH 8 0
5. pH 8 O 1 1 25 24 1 SIGMA Cat No P2069 7 3 1 5 m 1 000 200 gl 3 5 ml DNA 1 4C DNA F013F0 W DNA 1 2 10 11 10 50 mg 1 5 ml
6. 1 5 ml 720 ul DNA F01301 W E041 8O 1 2 65 30 9 5 10 4 C 1 1 v v 500 9 15 OOO rom lt 20 25C 10 500 1 15 ml 500 ul 15 OOO rpm 10 7O 1 000 gl 15 000 rpm 10 50 1 TE PCR 6 DNA F013F0 W DNA 1 5m 1 10 50 mg DNA W 720 1
7. DNA DS OOO9 DNA Ver 1 0 RIZO Inc DNA DNA OO 0 0 OO 0 WW Ww DNA F013F 01 W DNA DNA DNA PCR DNA DNA 4 W 85 mL 110 2 10mL Xx 1 5mL X 2 4C
8. 8041 8Oul 65C 30 lt 20 25 C 5 10 50O1 1 1 v 15 OOO rpm 10 20 25 YT 5001 1 5m 1 500 ul 15 OOO rpm 10 G 1 1 OOO gl 70 15 OOO rpm 10 DNA DNA F013F0 W DNA D VA 1 W DNA W
9. W DNA 3 18S rRNA 500 bp 40O bp 214 bp PCR PCR 18S rRNA 500 bp gt 18S rRNA 400 bp gt 18S rRNA 214 bp gt 3 0 Agarose M Marker 500 bp 40OO bp 2OO bp 10OO bp 1 5 2 6 3 7 4 8 1 7 3O mg 8 10 mg DNA 8 DNA F013F0 W 3 18S rRNA 5OO bp 4OO bp 214 bp PCR 5OO bp 214 bo
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