Home

Protein Quantification Kit-Wide Range

1. pH Buffer solution pH Standard BSA solution WST 8 Buffer solution 100pl 100 100 100 PD 174 178 1 2 1 16 10 000 ug ml Standard BSA solution 1 2 1 Standard BSA solution 1 2 0 1 5 000 ug ml BSA Fig 3 2 Buffer solution 180 pl 3 1 BSA 20 ul
2. Table 1 Fig 5 Standard BSA solution Table 1 Chemical Detergent Bri 35 Brj 56 Bij 58 Triton X 100 Triton X 114 Tween 20 0 5 Tween 80 0 3 SDS 1 CHAPS 4 CHAPSO 2 MEGA 10 0 5 Octy B D glucoside 0 5 Organic sovent Ethanol 10 lsopropanol 10 DMSO BSA 5 Chemical Concentration Chelating agent EDTA 2 5 mmolll DTPA 0 625 mmolll Salt Sodium chloride 0 5 molll Potassium chloride 1 mo Sodium acetate 0 2 molll Sodium bicarbonate 6 25 mmol Concentration 2 1 1 1 1 Buffer Citrate pH5 0 0 6 mmoM MES pH6 1 12 5 mmol Tris pH7 4 2 5 mmol PBS Undiluted HEPES pH7 5 12 5 mmol CHES pH9 0 12 5 mmol BSA Table 2 Table 2 MY BSA ChymotypsinogenA Human l9G
3. Lowry Bicinchoninate BCA Biuret Bradford Lowry BCA Lowry Folin BCA Tetrazolium salt Tetrazolium salt Formazan dye WST 8 Formazan dye pH pH12 5 650 nm 50 ug ml 5 000 ug ml ww O KN O O Absorbance WST 8 WST 8 formazan Fig 1 WST 8 Formazan dye Wavelength nm Fig 2 WST 8
4. a b BSA 2 000 ng ml lt 500 tests gt WST 8 SolUtO RARE 10 ml x 1 Buffersolutigh ei 100 ml x 1 Standard BSA solution 10 000 ug ml 1 5 ml x 1 lt 2500 tests gt WS I 8 SOIUtIOM RE 50 ml x 1 Buffersolutioh See 500 ml x 1 Standard BSA solution 10 000 ug ml 1 5m x 2 0 5C WST 8 solution 0 5C 12 3 Buffer solution 18 pH BSA solution 0 5 C 12 650 nm 96 20 ul 200 pl 1 5 ml Table 2 Buffer solution
5. 0 2 Buffer solution 2 25 ml 0 1000 2000 3000 4000 5000 3 1 BSA 50 Concentration of BSA ug ml hl Fig 4 Standard BSA solution 4 WST 8 solution 250 5 37 1 Buffer solution WST 8 2 6 1cmx1cm 650 nm 7 BSA 0 ug ml 8 BSA BSA Fig 5 9 0 5 Absorbance at 650 nm Absorbance at 650 nm 0 1 0 1000 2000 3000 4000 5000 Concentration of BSA ug ml
6. gt gt Dojindo Molecular Technologies Inc 30 W Gude Dr Suite 260 Rockville Maryland 20850 2025 5 861 2202 Tel 1 301 987 2667 Fax 1 301 987 2687 URL www dojindo com Tel 096 286 1515 Fax 096 286 1525 URL www dojindo co jp Tel 03 3578 9651 Fax 03 3578 9650 PQ02 Proteostain Protein Quantification Kit Wide Range
7. n 3 4 WST 8 solution 20 pl 10 000 ug ml BSA 100 Wl ddH O 100 pl 5 37C 30 5 000 2 500 1 250 625 313 156 78 0 ng ml BSA Buffer solution WST 8 Fig 3 BSA 6 650 nm 2 7 BSA 0 ug ml 8 BSA BSA Fig 4 9 1 5 2 1 Standard BSA solution 1 2 0 5 000 ug ml BSA

Protein Quantification Kit-Wide Range

Contents

Download Pdf Manuals

Related Search

Related Contents