排出ガス、ばいじん及び燃え殻の ダイオキシン類簡易測定法マニュアル
Contents
1.2. 3 3 1 1 E H E
3. 19 RE NE 1 NN SENSEe NNE SGS SC SR 1 a 1 3 1 4 3 2 6 NN 6 SN 10 14 3 29 1
4. d 2 d a
5. 4 5 A JIS 4 5 TEF WHO TEE 2006 H 201 6
6. NN No 5 5 5 6
7. 3 3 1 3 2 1 1 2 3 4 5 6 2
8. 1 1 2 1 a b c 2 a b c 3 1 1 a 3 4
9. 25 b 2 a b c JIS Z9021 pt2co un3o tut2o 4 7 6 1
10. 1 2 3 4 5 5 5 1 5 4 4 4 4 7 5 3 5 5 5 11 5 8
11. 10 1 1 2 3 H1L6 1c2 17 92 1 1 1 1 1
12. f 17 92 1 6 9
13. 3 3 4 1 2 3 4 5 6 7 6 9 10 11 12 13 14 15 16 17 TEF 2 3 7 8 TeCDD Toxicity REquivalency Factor 2 3 7 8 1 TEQ 2 3 7 8 TeCDD Toxicity ERquivalency Quantity
14. HRG 6 PU C HRMS JTS K0311 j KO0311 HH
15. kn Y 2 2 1 1 6 3
16. Me Oo E
17. 3 2 4 2 I 2 pg well m3N ng m3N uL well mL mL mL 0 15 0 5 4 12 10 20 0 08 1 0 01 3 2 5 1 N 7 Aa 0 LT 6 MR ea 8 0 ml mL be ng 5 0 15 0 5 5 100 10 10 0 2 1 0 01 3 2 6 Hy 3 Si PL pg well m3N
18. HRGC HRMS 6 JIS KO0811 JIS K0811 4 1 4 8 5 8
19. pj
20. Bsd 1 PBSC 80 F 10mL 0 5 1mL 4 1 96 3 2 10 Bsd 10 000 20 000
21. 17 92 2 3 203 1 2 3 4 5 6 4 MVNO0020020008 203 4 203 i 204 OSGSGNIS GDH 206 R NAKAP RNORO RRB SPINN BE NSIM R3DH RNNKSSS NEEDS 213 DNSUN2UORSIR2 SRA BUUPDU MULENDPLPMIEDOPPeb2 1 225 17 92 2 4 227 RG ELGG 227 Se NN ED 227 3 228 SS SS SES RR 231 NN CR NG et 238 6 NANA S
22. 1 4 IT 3
23. vy mL Ve GmL Vg Gn Cpz ng TEQ g 4 8ng TEQ g 0 1ng TEQ g 20mL 10mL 1mL DMSO 0 018ng mL 0 595 W 0 018x0 595 x1x x 0 22 3
24. 96 96 E 15 96 96 15uL well 96 96 30 96 96 88 9 8 10 8 2 8 2 8 2 a a 8 2 oie oi en 0 00 0 00 0 00 a 1 2 3 4 5 8 7 0 0 2 0 4 0 A DMSO pg
25. 2 2 1 2 3 4 7 8 2 4 5 2 4 5 1 5 2 x10 5mL 3 45mL 4 2 4 5 TCPHA 4mL 5
26. 19 21 20
27. 2 7 1 5 1 1 1 1
28. 7 3 8 12 HRGC HRMS y 0 319x y 0 390x ng m3N ng g 0 319 0 390 2 2 93 8 6 1 HRGC HRMS HRGC HRMS
29. JIS K0311 5 4 1 4 3 5 8 HRGC HRMS 112 6 n 54 n 20 HRGC HRMS HRGC HRMS
30. 4 4 1 4 4 1 2 1 2 B F
31. 16 7 2 JIS K0811 70 130 JTS K0311 1 4 2 1 1
32. ug mL HRGC HRMS ng TEQ mL 4 4 17 n 23 n 27 12 12 y 1 221x y 1 267x 8 10 R 0 946 10 R 0 971 8 8 i 6 1 6 1 1 4 4 3 3 e 2 2 e 0 0 0 2 4 6 8 10 0 2 4 6 8 10 ng TEQ g ng TEQ g 4 4 17 252
33. DMSO DMSO 2 5 4 192 1 2 6 4 192 1 2 7 4 192 2 8 4 192 4 192
34. n 27 n 20 ng mL HRGC HRMS ng TEQ mL 4 1 13 n 27 100 y 0 0601 x 1000 7 7995 r 0 9415 10 F IM 0 1 0 01 100 1000 10000 100000 1000000 ng m 4 1 18 180 i Lr T nT 2
35. 3 1 1 4 DMSO 5 4 4 1 1 2
36. 1 1 2 3 2 1 2 3 3 1 2 3 4 1 2
37. 100 pg7mL 3 3 1 3 7 8 TeCDD DMSO 3 DMSO 2 1 1 10 20 30 pg mL 2 RLU 3 2 4 3 3 8 3 5 2
38. n 94 n 20 HRGC HRMS HRGC HRMS 1 X HRGC HRMS Y Y X 2 Y X 8 1 16 Y X 0 318 20 15 y 0221x 3 y 0 318x R2 0 993 G R2 0 899 amp 10 Le 5 0 0 20 40 60 80 0 50 100 150 ng m3N ng g 3 1 15 8 1 16
39. 17 9 14 18 3 23 20 3 17 22 3 31 17
40. 2 2 1 Y gy GS Y ngTEG msN ks ngTEQ ngDEQ X ngDEQ msN 2 Y kg 0 0494 Y y kaxX ee ngTEGQ g ka ngTEQ ngDEQ ka 0 0523 X ngDEQ g 8 6 1 HRGC HRMS HRGC HRMS
41. 3 1 JIS R3503 JIS R3505 3 2 ASE 200 ASR 3 3 JIS R3505 3 4 KD 3 5 14mm 300mm 3 6 6mm 50mm 3 7 100
42. Cp O RC DL Vz V Co Oo RS Vs VV GS Cpz ug m3N ug g Coz ug m3N ug g pz ug mL ez ug mL 0 40mL 0 02 mL 20 2 mL Vz mL Vz ml m3N gd pz gz 5 6 1 6 1 2 4 4 10 0 0022ug mL 0 0038ug mL
43. 1 50 pg mL pg mL 3 5 13 65 60 on 23 78 TeCDD pg ml Cm a 35 0 5 10 15 20 25 30 3 5 18 5 3 1 RLU ON 3 2
44. 1 X HRGC HRMS Y Y X 2 Y X 0 319 3 3 12 Y X n 44 n 20 HRGC HRMS HRGC HRMS 1 X HRGC HRMS Y Y X
45. STD5 TeCDD 0 78ppt QC 8 1 12 1 4 2 STD5 TeCDD 0 78ppt 5 3 STD5 TeCDD 0 78ppt 3 1 6 49 RLU 3 1 10 TeCDD TeCDD C LN B 100 8 1 10 11 0 IE3 i 8 1 11 3 QC 5 3 4 QC PCDDs PCDFs 0 607
46. 17 92 2 1 160 BEO NANAMAKNKMWNNSOSUONS RGRSASR De 160 RR SS GEAR EAE 160 3 161 4 0 ANN a 162 RRDIEEYS SSSSE9 SS SS 3 168 NASORASRKR NANA CN SR0SSRRSOIRSSSSSRSGRBSSe5RRDS 180 2 17 92 2 2 181 1 ASA 181 SS RRR RNR 181 3 0 182 AS 184 190 9 202 3
47. 6 HRGC HRMS JIS K0811 JIS K0311 4 1 4 3 5 3 4 192
48. 1 2 1 1 30 3 Un VENER Ve 1 1000 V s Cp V m3N z pg mL 6 1 2 6 1 2 vy mL
49. 1 2 1 1 14 1 3 1 4
50. 0C 101 32kPa g m9 0C 101 32kPa g m9 gmL E og mL H 4mL mL 4 d d 50mL 4 d 1mL 53 0 977pg mL 0 195pg mL mL mL 10mT V 0 101 32 kPa 4m k 0 221 2
51. 44 20g 45mL 44 3 1 1mL 2 2mL 2 2 80C 60 3 5g 4 30mL 2 3 4 1 3 1mL
52. 17 92 1 6 1 Ah 83 6 1 83 6 1 2 1 guinea pig 2 Ah Arylhydrocarbon Receptor
53. 224 6 ng mL HRGC HRMS ng TEQ mL 4 3 16 n 20 y 0 4224x R 0 9101 ng TEQ ml 4 HH ng ml 4 8 16 ng mL HRGC HRMS ng TEQ mL 4 83 17
54. 5 5 1 1 6 3 3 4 4 77 2 2 4 3 1 77 2 DMSO 4 3 1 STD1 STD5 77 2 100 DMSO 4 8 1 Ipg7el 200 0 1 O 3 1 0 Ln 5 s 1 los oi o 3 77 2 450nm 77 2 4 A D 44
55. 1H 3 2 4 pH 7 ASE J 8 2 4 ASE 1 pH pH pH 7 pH 7 2 3 ASE
56. 2 3 1 1 4 4 363 nm 447 nm nmol L s Rate 4 87C 5
57. un 2o n o 1 5 1 10 ng mL ng TEQ mL 83 2 9 70
58. 3 4 2 2 1 JISK0557 A4 A3 2 JIS K8040 3 JIS K8680 4 JIS K8117 5 JIS K8825 6 DMSO JIS K9702 1 7 JIS K8987 8 JISK8180 9 JIS K8951 10 1 6 11
59. 16 12 2 000kg h E 17 9 F 11 HT E 67 17 92 4 22 3 6 E
60. 1 2 3 4 5 1 2 2200 4 5 160 9 9 0 mW 0 0 IT2 ol292 9 129 145 152 5 oe 1 9 9 4 LAL24L 24 Ia2 0240 124 84 ed 2 Lee SEMSU0U LLS IS eo6l56100 ee6 D6 IRM2IPMSIT2 022 owla2190 127 47 57 157 6 8 CS 8 8 4 1 7 96 8300nL 4
61. 6 4 10 100uL wel 4 11 24mL 7mL 300uL well 3 18 25 80 100uL well
62. 10 Gene Transcription DNA RNA 11 CYP1A1 Cytochrome P450 P450 CYP1A1 12 Subculture a 13 Confluent 14 Plasmid DNA 15 16 CV 1 3 1
63. lt H 4 1 6 5 167 5 25 a HRGC HRMS 90 NS RE i EN 30 suman Uae inoms 1 2
64. 1 1 5 1 HRGC HRMS 3 HE 7 ar
65. 4 1 4 3 5 3 74 6 n 32 n 20 HRGC HRMS HRGC HRMS 1 HRGC HRMS Y Y X 2
66. 200 mL 2 G 3 E E B 10 12 10 18 F 1 9 E 1 9 B 1 9 E E o o I 200 nL a
67. SS 214 4 4 1 1Cso ICso 4 3 10 1 DMSO 10 100 2 4 4 n 1 B Bo 3 1Cso DMSO c B Bo 100 50 B B 1250
68. 17 92 2 1 h H 17 92 2 7
69. 3 2 1 3 4 2 8g 44 10 4g 0 2g 20 3 6g 1 5g 3 5 6 121 2 1 5g 0 2g 20 3 6g 44 10 4g 3 5 6 3 5 1 0g
70. 8 5 5 1well g E ng g pL well mL mlLl 1 5 100 10 10 1 0 002 3 5 6 1 well 2 g E ng g IL well mL mlLl 1 5 100 10 10 1 0 002 3 5 7 1well msN E ng m3N IL well mL mlLl 1 4 12 5 10 1 0 01 3 5 8 1 well 2 g 26 E ng g pL well mL mlLl 1 5 100 10 10 1 0 004 3 5 9
71. 1 1 2 3 4 5 4 1 2 3 4 5
72. 4 3 4 _ Op 1 1000 Wz 6 VV m3N zz og TEQ mL 12 mL mL mL Cpz ng TEQ m3N 4 DMSO 1 6 JIS K0311
73. Ligand Ioni Luciferase Gene CYP1A1 Cytochrome P450 P450 CYP1A1 XRE Xenobiotics Responsive Element Plasmid DNA 57 7 Subculture 8 3 1
74. 20mL 10mL 0 9mL 0 0022ug mL 20 1 49ug ng TRQ 0 9mL 1 22ug ng TEQ 3 2 1 1 30 3 Qp XN x vV Ve 1 0 g zz ug7mL DMSO
75. a R 3 1 4 4 3 EE 1 3 1 5 3 1 M Chuet al Methods and apparatus for separating and detecting specific polyhalogenated diaromatic hydrocarbons US Patent 6 720 431 2004 35 _ _ 1 1 8 38 1 5 1 1
76. ng7m3N ng g Vi V Cs XX VV Cs ng m3N ng g X ng mL Vg GQnD 174 mL vy mL ms3N g x C 1 0 EC On Os 6 Cs ng m3N 6 6 1 2 2 STD1 6 3 H
77. E E H 1 1 1H oe 1 HRGC H
78. 83 30mL b C 44 100mL 9 2g 2g 44 8 10g 1g 2 3g 1g 10 7 5g 1g TTY i j
79. 11 6 8 1 3 1 IRO SA 4 8 TeCDF 0 1 2 3 7 8 PeC 8 PeC gt CC CO TT 7 4 6 7 6 4 4 8 TeCDD 1 pa 7 8 PeCDD gt 4 7 8 HzCDD 6 7 7 8 9 HxCDD 4 6 NKK 8 HxCDD gt NAN gt 7 8 HpCDD 0CDD otal PCDDs al PCDFs PCDDs 3 4 4 5 TeCB 81 4 4 TeCB 77 4 4 5 PeCB 126 4 4 5 5 HxCB 169 4 4 gt 4 5 PeCB 123 4 5 PeCB 118 3 3 4 4 PeCB 105 3 4 4 5 PeCB 114 IiH NE NNAH 3 4 4 5 5 HxCB 167 3 3 4 4 5 HxCB 156 2 3 3 4 4 5 HxCB 157 2 3 3 4 4 5 5 HpCB 189 Total PCB 3 3 3 2 2 2 2 2 2
80. H1L6 1c2 17 92 1 11 29 RS 29 8 29 3 30 NN NE RBOE YS 32 5 ER RR 39 6 3 GR ER RE 56 2 101L 17 92 1 2 ii 57 ni 00Yao2 ce 57 Na 57 3 58 0 NO MRIISSNE MNOSRERSSSSSR NERO 0223 59 SN RE 65 EC DS a a 3 75 3
81. 1 2 1 1 30 3 ko Vg Cp V m3N zz ng TEQ mL DMSO vy mL Vz mD mL zz ng TEQ m3N 4 5ng TEQ m3N 0 17ng TEQ m3N 20mL 10mL
82. 2 7 2 70 60 5 150C 2000psi 120 4 3 3 5 5 60 C 90 C DMSO 3 5 5 1 1 1 0m3N 1 0g 1 2 0 4 mL
83. E E 1 9 Yi y 40 mL 30 mL 4 1 3 1 mL 3 2 3 IE DMSO 0 5 mL UE 5 1 5 5 2 4 5
84. 3 2 3 5mL 3 60 10 236 4 60 85 mL 2 5 mL min 60 C 4 4 8 4 1 3 2 90 10 3 90 3 4 DMSO 2 5mL 2 5mL mi
85. 216 450nm 4 4 15 4 12 1 2 3 4 5 15
86. 50 DMSO 8 TCPHA 1 9 10 11 EE 2 gt 1 i E 12 ul
87. 2 1 2 3 6 8 1 m N ED 4 EF
88. 82 4 3 3 3 5 83 3 5 1 11 1 10 2 1 a 15mm 3 3 6
89. 195 FT T FT LN B E REJECT 1 0 REJECT CAL 2 1 mL HL 3 125 mL HL 4 mL HL 1169 mL CHL mL 2 4 4 CCEP 17e 2 X 3 SE 2 749e 1 X 2 31e g X NEXT CELTB CHLIB NHLYTE_SEL SEL ECCEPT CHLTB CHL TB CRLCULRTE HHRP CEEY CUPY 4 2 9 4 1 11 SSHT_ DAHTE i CE 4 1 9 CHNC LCHEHTE 4 HHRP CEEY 4 2 2 4 2 9 B Bo TCAP ate sp cv mp ug mL 0 0 46 16 1 0 0 48 40 48 24 2 00 4 1 100 0 0 50 15 41 9 41 87 2 41 9 39 60 41 02 1 24 3 0 85 41 9 41 60 125 0 32 43 3 125 0 32 44 32 67 0 40 1 2 68 125 0 33 13 373 0 18 64 4 373 0 19 40 19 27 0 58 3 0 40 373 0 19 79 1 169 0 7 62 5 1 169 0 7 62 7 72 0 17 2 2 16 1 169 0 7 91 2 885 0 2 61 6 2 885 0 2 60 2 64 0 0
90. 1 2 Q 1780 3 V Ox Xk XT EX eV m3N zz ng mL DMSO vy mL Vg mL Vs mL Cpz ng TEQ m3N 4 5ng TEQ m3N 0 17ng TEQ m3N 2
91. 1 2 5 10 ng mL 1 2 68 3 3 4 2 4 2 STD 50 pL 2 1 STD 2 5 3 2 7 6 16 0 5 3 2 1 1 5 25 125 625
92. 223 V ng TEQ g Qx0 522 x 2 ng mL Ve mL Vs mL vy mT 2 2 8 6 1 HRGC HRMS HRGC HRMS 6
93. xkx V 1 _ Qpi Ee 1000 V s Copi V m3N zz og mL DMSO 2 mL Vg GmL VW GmL Cpr ng TEQ m3N 4 5ng TEQ m3N 0 17ng TEQ m3N 40mL L H 10mL
94. 22 3 22 tT 3 31
95. EE a 6 1 HRGC HRMS Ll a tz ng g ng m3N x 2 2 E 6 HRGC HRMS 4 JIS K0311 1 fl ED
96. ng TEQ well g mL mL mL Qg TEQ g 0 00023 25 10 0 10 20 1 0 1 0 0 0018 SS ng TEQ well i mL mL ml 5 ng TEGQ g 0 00023 25 50 0 10 20 1 0 1 0 0 00036 177 4 1 10 7 ng TEQ well we GaN Gabi mL agTEamm 0 00043 0 0085 DB 25 4 0 12 0 10 20 1 0 1 0
97. 114 6 Plasmid DNA 7 Subculture 8 3 1 1 2 1 1 30 3 Op Xkxv Vp 1 ul 0
98. 1 250X1X20 oo0 2 JIS K0311 3 4 4 1 5 4 1 5 166 1 JIS K 0311 6 4 4 2 2 a 10mL b 100mL
99. 4 1 4 8 5 8 55 6 n 46 n 20 HRGC HRMS HRGC HRMS 1 X HRGC HRMS Y Y X 2 Y X 8 1 15 Y X 0 221
100. 3 2 4 2 4 4 E 2
101. 4 8C 4 6 100L well 25 60 300uL 6 RE 171 4 7 100uL 25 30
102. 1 2 1 1 30 3 a Op Xk Ve Vi 1000 Cp Pc V s a m3N pr pgDRQ Cpr ng TEQ m3N Ve DMSO uL Vv e 1 DMSO uL 2 mL V s mL 4 0 1ng TEQ m3N 0 0033ng TEQ m3N 50 mL 25 mL
103. 1 Y HRGC HRMS X Y X 2 Y 4 2 13 Y X 175 5 x 103 n 52 n 57 100 000 y 208 732 9 y 183 966 x R 0 966 3 RS lt lt O 9 i 10 ng TEQ m N ng TEQ g 4 2 12 4 2 183 202 3
104. 2 3 Fc 4 5 6 B Bo 0 100 203 7 ICs 50 50 Inhibition Concentration 8 CV 3 1
105. 1m3N 0 9mL 4 4 4 H 2800uL DMSO 1000uL 4 4 4 2 DMSO 200 X pL a XuL 2800uL 1000uL 4000uL Y aX 0 0095 X a X ulL a 243
106. 193 OGDG9G9 CGG 4 2 7 5 DMSO 40 LL 160 pL 1 6 5 20 DMSO 1 6 50 pL 50uLL 10 37 37
107. 9 SS fF 18 19 20 21 22 RLU Relative Light Unit HRGC HRMS JIS K0311 16 80
108. 0 1 ng TEQ g 10mL 1mL 10pgmL 0 2759 0 2069 1 10 x0 2759x1 10 1 0 XX X 0 1000 10 0 1 2 10x0 2069X1 10 1 2 0 1000 10 0 1 116 4 1 3 5 2 3 5 2 2 HH Et 1 JISK0557 A4 A3
109. 3 2 1 3 3 2 3g 44 10 4g 0 2g 20 8 6g 1 5g 4 3 6 210 20 3 6g 0 2g 44 10 4g 2 3g gt 2 3 4 1 0 g 4 3 7 1 5 1 0g 443 7
110. 1 well 2 g ng7g LuL well mL mlLl 1 5 100 10 10 1 0 004 7 5 3 H HH k 2 2 133 8 6 MSN 1 1 HH EH HRGC HRMS f HRGC HRMS DI JI8 KO0311 HRGC HRMS
111. 2 1 3 3 2 3g 44 10 4g 0 2g 20 3 6g 1 5g 4 4 6 235 20 3 6g 0 2g 44 10 4g 2 3g gt 2 3 4 1 0g 4 4 7 5 1 0g 4 4 7 1 4 4 8 2
112. 13 mm X100 mm 300uL 1 2 2 1 2 5 1 1 6 B Bo 50 2 1 1 194 7 3 2 1 04 1 2 OOO 4 2 8
113. 2 2 1 H4TE 1uc DRE 4 P450 CYP1A1 pGudLuc1 1 H4LE 2 2 PM Garrison et al Species Specific Recombinant Cell Line as Bioassay Systems for the Detection of 2 3 7 8 Tetrachlorodibenzo p dioxin hke Chemicals Fundam Appl Toxico1 1996 Apr 30 194 203 2 2 1 a MEM FBS 10 2 Fetal Bovine Serum _ 20 3 HBSS 4 0 05 20 5 PPBS 6 DMSO JIS K9702 1 7
114. 2 2 8 6 1 HRGC HRMS HRGC HRMS HRGC HRMS 4 111 E 6 JIS K0811
115. 300pg mL 0 192 _ 300x0 192x0 2 10 1 0 1000 10 0 1 2 4 1 3 2 2 3 2 2 59 2 JIS K0311 ff 1 DMSO JIS K9702
116. HRGC HRMS 6 158 6 1 N 20 DEQ HRGC HRMS TEQ YX 2 3 6 16 42
117. 200zL 2 30 60 405nm 30 4 5 1 AB1 AB2 2 ELISA 3 5 5 1 1 3
118. 450C JIS R3503 JIS R3505 3 2 JIS R 3503 3 3 KD 3 4 0 25MPa HH 163 3 4 4 1 4 2 1 JIS K0311 6 4 4 1 8
119. 2 2 2 192 2 2 3
120. my 0C 101 3kPa 760mmHg pg Microgram 100 1g 10 6g ng Nanogram 10 1g 10 9g 23 24 25 26 27 28 29 30 4 pg Picogram 1 1g 10 12g 4 E
121. 6 E HRGC HRMS JIS K 0811 JIS K 0811 4 1 4 8 5 8 4 192
122. 100uL 4 8 450nm 5 5 5 4 9 1 2 3 5 4
123. 2 3 7 8 WHO TEF WHO IPCS 2006 TEF HRGC HRMS 10 000 2 3 7 8 TeCDD 2 3 7 8 92 3 7 8 PCDD PCDF PCBs 2 2 6 6 1 PCDD PCDF
124. 1 2 1 1780 5 ng TEQ m3N 0 17 ng TEQ m3N 3 Qp Xkxv 1000 Qpr V 14 Vr VE Cpz 1 1 H mL 3 m3N pg mL DMSO mL
125. 100mL c 4 1 6 d e 60mL f 2 mL min 9 h 60mL 2mL 2
126. 4 4 1 5mL 2 3mL 3 2mL 25mL 4 2mL 5 6 2mL 7 1mL 8 10mL 9 PCBs 10 10 80 15mL PCBs 10 20mL PCDDs PCDFs 11 Q
127. 6 1 50 120 5 11 5 1 5 10 5 12 28 HRGC HRMS ars 1 E i
128. XX 3 Y Y pgDEQ well mixX3 mzxX2 maxX b mi ms ms b 2 X 2 1 Ve Vg 1 1 C X7 Ve Vp V 1000 Cs ngDEQ m3N 1 pgDEQ well pw DMSO GnL 0 04 mL 40 pL ys 1 DMSO mL 0 002 mL pL mL gz mL m3N 156 2 Vs 1 1 C re P Pr W 1000 SE Cs ngDEQ g 1 pgDEQ we pe
129. 1 4 3 4 1 6 4 5 ng TEQ mL 0 0669 x gt 1 E ng7mL 7 1000 0 8301 175 E n 5 7 4 1 11 4 AD F o LLT E
130. 4 8 1 2 1000 rpm 5 12 mL H G418 0 4 mg mL 2 2 mL E 2 1 1 F 2 E 2 1
131. Q 5mL 8 3 Q15mD 500rpm 10min 4 8 5 10mL 5mL 8 6 25cm2 8 7 COs 5 37C 2 25cm2 75cm2 150cm2
132. 2 Ll 5 5 HRGC HRMS 6 6 1 HRGC HRMS
133. DMSO DMSO 84 5 1 HeB5 Ah 2 8 7 8 TeCDD
134. 1 2 1 1 80 3 ul 115 Op XkxXv Vp 1 1000 Ve Cp g zz pg mL DMSO Vv mL Ve mL Vs mL Czz ng TEQ g 4 3 ng TEQ g
135. 4 192 HH I 1 2 E HRGC HRMS HRGC HRMS 3 i 100 30 0 9 HRGC HRMS
136. 1 488 ug ng TRQ 1 267 ug ng TEQ 4 4 183 g TEQ m ng TEQ g Cs TCPHA 11 TCPHA 8 6 1 HRGC HRMS HRGC HRMS 1 221 ug ng TEQ ug m3N ng g 11 ug ng TEQ 5 3 E
137. 6 2 2 1 3 2 2 4 6 1 3 2 17 92 1 4 HRGC HRMS 3 4
138. 1 HRGC HRMS Y Y X 2 Y X 8 4 10 Y X 0 308 n 41 n 20 HRGC HRMS HRGC HRMS 1 HRGC HRMS Y Y X 2 Y X 8 4 11
139. 1000uL 3 4 4 2 2 1 2 3 DMSO 200 0 5X OuL 200 X ulL 200 1 5X LL 0 5XuL Xu 1 5XuL 2800uL 2800uL 2800uL 1000uL 1000uL 1000uL 4000uL 4000uL 4000uL 2 242 _ 0 0095 xV 5 C uL uL C TCPHA ug mL LU 3 0 089ms 1g 0 9mL 6 4 4 3
140. 181 5 B B B Bo 6 CV 7 TCAP 5 5 4 5 3 1 1 2 1 1 30 3
141. 8 DMSO 40 pL 20nL un 144 9 4 4 H 4 3 3 6 6 60 C 90 DMSO DMSO 3 6 6 1 1
142. 5 5 1 1 1 2 6 B 1 B B B y x Logit Log Logit Log Y AX3 BX2 CX D Logit y A Log x 5 3 B Log Gx 5 2 C Log x 5 D Y Logity y B Bo A B C D X Log x 5 x ug mL 2 60 60 3 4 2 9 B Bo 4 2 2
143. 4 4 1 4 2 1 JIS K0311 6 4 4 2 8 185 HCI 2 mol L 1 g 20 mmol L 16 2nn 3 4 2 3 186 2 4 192 4 2 4
144. Qpr XV 2 k Vg Cp VV m3N zz ng TCAP mL DMSO Y k vy mL Ve mL Vs H mL Cpz ng TEQ m3N 4 5 ng TEQ m3N 0 17 ng TEQ m3N 20 mL 10 mL 0 5 mL 26 3X103 ng TCAP mL
145. n 52 6 1 n 6 HRGC HRMS HRGC HRMS 1 Y HRGC HRMS X Y X 2 Y X 4 2 12 Y X 200 6 x 103 n 57 6 n 6 HRGC HRMS HRGC HRMS
146. 17 JIS K0311 6 2 18 JIS K1107 1 19 JIS K8251 3 3 1 JIS R3503 JIS R3505 3 2 ASE 200 ASR 3 3 KD
147. 2 3 1 DMSO F 4 192 1 Eo HH 1 ud x 3 1 JIS R3503 JIS R3505 3 2 1 JIS R3503 3 2 2 ASE ASE 300 ASE
148. KD eid 80C 4 4 1 100 4 2 1 JIS KO0811 6 4 3 4 3 HCI 2mol 7 1g 20mmol 16 2 3 3 4 8 2 4
149. 2 Bsd 10 100mm 10mL 37 37C H Bsd PBSC 2mgmL 4mL Bsd 16ng mL 1 2 80 100mm a 3
150. i SC oe 3 3 6 2 10mm 10g 3 Q 0 5um 8 3 5 4 DMSO 3 50mL
151. 0 066 ng TEQ m3N 0 13 7 0 ng TEQ m3N 2 1 3 2 1 5 g 10 mL 10 mL 0 5 mL 0 050 ng TEQ g 2 4 2 9 0 096 5 3 ng TEQ g DMSO 5 3 81 81 430 ng TEQ g 200 4 2 9
152. 101L 17 92 1 9 3 HeB5 92 1 3 17 4 H 17 92 5 6 2 3 4 H4IE luc a DR EcoScreen 17 1 92 1
153. A 2 JIS K0095 JIS K0211 JIS K0215 JIS K0301 JIS K0311 JIS K0901 JISR3503 JIS R3505 JIS Z8808 JIS Z9020 JIS Z9021
154. Vy mT Vz mL Vz mL Cpz ng TEQ m3N 4 5ng TEQ m3N 0 17ng TEQ m3N _ 0 0022 Xx 20 x 0 9 20 1 XX X 0 31 1 49 10 0 17 2 1 2 1 1 30 3 xnxv WV 1 Qp 3
155. 17 92 2 4 3 3 1 2 3 4 4 5 1
156. 17 92 1 5 114 SG SIG 114 NG 114 3 115 lt RSS SS SS CDE28 IA 117 NS CR CN RR KTS 124 6 RR 135 6 17 92 1 6 137 CC CC 2 137 SE GR RO RE RE ES 137 3 138 9 0OIAU NNN i 140 EC 148 6 CR 159 4 160 1
157. 3 1 13 1 2 3 4 5 6 7 8 9 10 11 12 A B C D E F G 7 1 7 nn 1STDO 7 H 3 1 13 3 1 9 8 1 14 pg ml n 5 30 20 0 977pg ml 20 1 95pg ml 52 3 1 9 0 977 Dg mL n 5 1 95 62 5 g mT n 5 pg well i pgTEQ m B C A 190 1000 LN B 100 CRLU 9 C V 47 5 250 10 13 24560 257 173 CLS 1 03 23 8 125 9 43 23926 189 106 56 0 1 51 11 9 62 5 8 74 19727 62 2 11 6 7 6 1 00 5 94 31 3 8 05 15002 31 0 5 54 7 0
158. 0 050 ng TEQ g 0 096 5 3 ng TEQ g 7 5 3 8 6 1 3 200 6x108 3 175 5x103 ng TEQ m3N ng TEQ g ug TCAP m3N nug TCAP g 2 2 JIS KO0311 K0311
159. Wr o 5 4 4 11 5 1 TCPHA 6 1 TCPHA 4 4 5 TCPHA TCPHA STD7 20ug mL STD1 STD6 2
160. 2 3 1 RLU 5 5 1 1 STD 2 3 7 8 TeCDD 3 5 11 X RLU Y 2 STD 20 100 pg mL 20 30 50 100 pg mL 3 5 12 128 160000 140000 120000 100000 80000 60000 40000 20000 0 T T T T T T T T T 0 100 200 300 400 500 600 700 800 900 1000 TCDD pg ml 83 5 11 2 3 7 8 TeCDD STD 80000 70000 y 656 8x 8814 R 099
161. 2 20uL 2 1 a 3 4 1 7g 33 3 3 0g 1 7g 8 1 6 b 830mL ft 36 1 7g 33 3 7 1g 3 0g 3 1 6 2 a 3 5 1 7g 33 3
162. 3 3 1 A RN 2 5 1000 rpm 5 3 2 0x106 cells mL 3 126 5 4 5 4 4 1 1 2 1 2 3 4 E 6 3 5 1 3 2 90uL 1 5 1 1mL 5 80C ADS 24
163. b E 1 2 3 We 1 1 1 3 9 O
164. n 24 n 20 HRGC HRMS 1 ra 135 HRGC HRMS 1 X HRGC HRMS Y Y X 2 Y X 3 5 16 Y X 0 2069 HRGC HRMS ng TEQ g 9 O 8 y 0 2069x O 7 R 0 919 HRGC HRMS ng TEQ g 0 5 10 15 20 25 ng g 8 5 16 0 30 35 0 5 10 15 20 25 30 35 40 45 ng g 136 6
165. 4 1 4 3 5 3 El 179 6 n 29 n 20 ng mL HRGC HRMS ng TEQ mL 4 1 12 n 29 100 Eg ng TEQ m lt 0 01 E y 0 0669 x 1000 8391 r 0 9783 0 000 10 100 1000 10000 100000 1000000 ng m 4 1 12
166. zz ug mL r mL Ve mn Vs mL Cpr ng TEQ g 4 3ng TEQ g 0 1ng TEQ g 0 0022ng mL 20 0 06 1 1 22 0 0 1 0 65 229 20mL 10mL
167. 1 2 6 1 2 2 5 2 3 2 10 70 3 1 1 25cm2 1 2 1mL
168. 5 2 1 E 26 2 5 3 5 4
169. 1 5 Q 1 H 1 17 92 1 6 2 1 F 17 3 92 2 1 1 H
170. 2 8 3 2 103 4 DMSO 50uL 5 5 1 H4TE Iluc Ah 2 8 7 8 TeCDD
171. 3 100 mL 1 5 1 1 mL 40 200 mmHg 2 3 35 4 5 0 4 mL 1 mL 6 7 DMSO 40 pL 40 50
172. 3 2 JIS R3503 3 3 KD 1 3 4 7mm 8300mm 3 5 183mm 300mm 3 6 6mm 8300mm 3 7 3 3 3 4 4 1 4 2 1 JIS K0311 6 4 3 1 3
173. 4 HRGC HRMS E FT gt HH 54 JIS KO0811 4 1 4 8 5 3 4 192
174. PO OnmGplAD 1 0 742 9 77 2 0 768 8 57 3 0 744 9 67 4 0 748 9 46 5 0 787 10 09 6 0 744 9 66 7 0 749 9 39 8 0 766 8 42 9 0 744 9 65 10 0 748 9 43 _ 34 0 741 9 81 ng TCPHA m 0 5 10 15 20 25 30 35 40 4 4 18 5 3 B Bo 65 80 B Bo TCPHA ng m3N ng g C Vp V 7 5 RN Os TCPHA ug m3N pg g TCPHA ug mL 5 vy mL Vg mL
175. 1 2 1 1 80 3 Qp 9 V E Cp Eo Qpr ng mL DMSO k vy GmL Vz mL Vs mL Cpz ng TEQ g 4 3ng TEQ g 0 1ng TEQ g DMSO 0 018ngymL H 0 018 x0 522x1x x 019 205
176. 13 mL 1mL 1 150cm2 3 2 8 2 50mL 3 3 Q0mL 8 H 20mL 8 4 4 1 0 4X106cells mL 1 2X106cells mL 8 5 1 1 8 6 1000uL 1 5mL 8 7
177. 5 15 25 30 6 15 25 30 15 25C 100 mL 15 25 3 2 8 7 7 STC 15 25 3 4 2 8C 15 25 C
178. A Vg OE NRN Vg 1000 Vy WwW 1000 V s W Chr ng g Coz ng g zz pg mL 0 977pg mL gz pg mL 1 95pg mL 12 4 d 1mL 4mL 47d d mL Vg mL 50mT GmL 25mT g 7g 0 318 7 8 gt 2 3 7 8 TeCDD HRGC HRMS HRGC HRMS oO mg TEQ g ng TEQ m3N
179. M wel u2o NT 3pM well 3 4 3pM well 3 5 gt 3E 2 5 T T 0 20 30 10 83 4 8 8pM well 109 8 5 3 1 5 1 2 DMSO
180. 3 6 7 E 2 1 0 g 3 6 8 gt 1 0g 3 6 8 3 1 3 6 9 2 5 mL 3 60 10 4 60 85 mL 2 5 mL min 146 4 1 2 3 60 EE
181. 1 2 3 EA par rE
182. 2 1 Luciferase Gene 4 2 Ah Arylhydrocarbon Receptor 83 4 1 E 3 Ligandg 4 DRE Dioxin Responsive Element 5 CYP1A1 Cytochrome P450 P450
183. 7 830uL 8 2 9 10 47 2 17 1 2 96 17 5 5 1 1 SS ki ks E S SC E P Kk
184. 8 HCI 2mol L 1g HCI 2mol L 10mL 16 2 3 gn 4 1 3 164 2 4 192 4 1 4 1g HCI 2mol L 10mL HCI 2mol L 16 2 3 9 4 1 4
185. 8ng TEQ g 0 1ng TEQ g 50mL 25mL 4mL 0 977pg m1l 0 318 5 0977x04x0 318x4_ 50 _ 1 1000 55 010 31 4 1 3 1 2 3 1 2
186. JIS K0311 10 11 4 12 JIS K1107 2 3 1 DMSO 3 1 1 1H
187. 8 8 200uL 8 COs 5 87C 14 24 44 13 cells mL 4x4 F x104 mL x cells 7 5x10 cells mL 4 2 4 2 1 1 1 a 18mm DMSO 2uL 1 40 4mL 1740 100uL b 4mL 12 14 3 1 5 4mL
188. 96well 1 20uL 20 200uL 200 1000uL 8 5 50uL 8 50 300uL 3 1 2 3 4 5 6 8 30 2 1
189. 33 HCI 2mol L 1g 20mmol H 3 1 8 2 lt E 192 4 H 34 16 3 3 1 4 HCI 2mol L 3 16
190. x10 239 2 8 2 x10 2 x1 1 3 2 1 4 TCPHA 5 5 4 4 1
191. 3 5 1 2 1 Ah Ah Receptor Arylhydrocarbon Recepto 2 rigand 3 Luciferase Gene CYP1A1 4 CYP1A1 Cytochrome P450 P450 5 DRE Dioxin Responsive Element
192. 64 9 KD KD 8 mL 10 80 mL 11 12 13 70 mL 14 0 5 mL 15 10 4 1 3 KD 20uL DMSO 20uL 20uL
193. 2 2 1 HeB5 XRE 5 pGL3 chTATA YaXREx5 bsd Hepa 1clc7 2 2 1 qMEM NaHCO3 2 Fetal Bovine Serum FBS 56C 30 20 3 CPBS 4 0 25 0 5 0 25 1mM EDTA 6 S Bsd 1 7 8 9 10 2 3 7 8 TeCDD 50ng mL DMSO 11 DMSO JIS K9702 1 12 JIS KO0557 A4 A3
194. 6 2 3 3 ei DMSO 3 J 3 6 12 18 4 DMSO 38 3 6 13 18 4 2 3 6 12 150 1 pg 3 6 13 2 pg 1 2 3 4 5 6 7 8 9 2 3 4 10 11 2 2 2 2 2 4 4 4 4 4 8 8 8 8 8 11 12 13 14 18 2 2 2 2 2
195. 2 3 7 8 TeCDD pM well 3 pg g pg m3N pg g pg m3N pM welDx125 x x x322 2 3 7 8 TeCDD x109 Dg pg g pg m3N 125 125 4 3pM 9 3 83pM well 2 10 p 4 X 3 ll y pg g pg m3N a 8pM well pM well A 3pM well pg g pg m3N b 8pM well pM well B 3pM well
196. 4 DEMS 300pM we11 8 3 3 1 90 5 3 2 1 6 2 1 5mL 2 3 2 3 3 4 4 5 5 5 4 10 3 10 5 DMSO 1 4 6 80 1 7 1 80C
197. E 0 6 JIS K0311 4 1 4 3 5 8 4 192
198. 3 DMSO 1 7 JIS KO0311 1 8 JIS KO0311 1 9 JIS KO0311 5 8 5 6 5 7 5 9 2 2 1 4 192 1 2 2 4 192 1
199. 0 033ng TEQ g 40 mL DMSO 1 pgDEQ 1 50 mL 25 mL 1 DMSO 2uL 0 0523 TREQ DEQ 1 DMSO 2 4 nL 2 nL x2 10 40 pL Sk Qpr 1 pgDEQ Cpr 0 033 ng TEQ g 1 30 0
200. 150 2 1 4 2 DMSO TCP 1Lmg mL 5 200ug mL TCP 0 2mL DMSO 0 8mL DMSO 4 7 50 12 5 3 18 0 78 0 20 0 05 TCP 0 2mL DMSO 0 6mL TCP 200ug mL gmL 200ug7mL 8
201. Bo 5 0 75mL 0 75mL min 6 7 4 2 2 0 4mL 0 75mL min 4 2 3 0 4mL 0 775mL min 8 9 B 10 mal 4 11 1
202. 12 PCBs DL PCBs mRNA DNA RNA DNA
203. 0 07mL 100pg mL 0 319 OT ee 50 1 1000 2 2 1 2 1 1 30 3 xkxv WV 1 os hs g zz gmL DMSO 12 mL mL
204. 3 1 2 1 3
205. Ww 14 mL Vg GmL Vz GmT Cpz ng TEGQ g 4 83ng TEQ g 0 1ng TEQ g 10mL 10mL 1 mL 0 0022ug mL 20 1 27ug ng TRQ 0 0022X20X0 9 20 1 0 62 1 27 10 0 1
206. 2 f 3 3 3 2 5 4 10mL 1 20 70 20 E 5 10 H 200uL
207. 221 CV 0 SN 1 EI 1 0 01 0 0350 1 1 10 lt ng ml 4 3 15 x i W EE LU 4 3 8 100 DMSO TR 1 10uL kt 4 3 8 _ 0 018 0 35 100 0 035 10 6 2 4 8 9 4 3 10
208. 230 4 1 4 4 2 4 4 2 2 1 JIS K0557 A4 A3 2 JIS K8891 3 JIS K8040 4 JISK8680 5 JIS K8117 6 JISK8825 7 8 DMSO JIS K9702 9
209. 234 60 C SR 90 C DMSO 4 4 5 1 1 1 0m3N 1 0g 1 2 0 4mL 3
210. 4 8 TCPHA 13 IC50 14 B B 3 1 HH 1 50 50 0 50 Inhibition Concentration E E 2 3 4 Co
211. 450 4 12 JIS K1107 2 3 3 1 JIS R3503 JTS R3505 3 2 ASE ASE 800 ASE 3 3 KD 1 3 4
212. 8 6 2 ELISA 153 3 6 2 30C 2 AB1 30C 1 AB2 30C 1 30C 30 60 2 3 1 880nL 2 2 3 Q 3 4 4 1 AB32
213. KO0311 6 4 1 K0311 6 4 2a 17 50mL 3 Np 2
214. b C a b C 2 4 10 80
215. 1 2 3 4 5 6 7 1 665nm 0 45um JIS K3802 1000uL 3 5 10mL JISK0970 JISK0970 650nm 3 F 200uL 1000uL 10 100nL 20 200nL 100 0 5mm
216. b c 170 i 4 1 1 4C 10 a 80uL b i 160uL 4 5 4C 10 n 2 100uL well 4 2 60 E 4 1 7
217. d e 0 5mL 60C 50mmHg 4 3 3 6 4 3 6 4 1 1 10mL 2 40 mL 3 5 4 3 5
218. 2 1 2 HEATTING 9 STATTC 10 FLUSHING 50 62 PURGING 300 200 C 1500 psi 4 5 6 10 mL 4 3 4 192 o 3 2 5 _ _ 1
219. 3 1 8 1 3 4 5 6 7 8 9 10 11 12 A 1 1 1 2 1 3 2 1 2 2 3 3 1 3 2 3 3 B 4 1 4 2 4 3 5 1 5 2 5 3 C F STD7 1STD1 7 12 1 12 2 12 3 13 1 13 2 13 3 G STD6 1QC 7 14 1 14 2 14 3 15 1 15 2 15 3 H 16 1 16 2 16 3 17 1 17 2 17 3 18 1 18 2 18 3 D 8 1 8 2 8 3 9 1 9 2 9 3 E STD8 7 STD2 6 1 6 2 6 3 7 1 7 2 7 3 10 1 10 2 10 3 111 1 11 2 11 3 3 1 8 15 B2 G2 B3 G3 B4 G4 B5 G5 QC NC 2 1 3
220. 1 2 1 1 30 3 xkxv WV 1 0 V m3N zz pg mL DMSO V vy mL Vg GmT mnL Cpz ng TEQ m3N 4 5ng TEQ m3N 0 17ng TEQ m3N 50mL 25mL
221. 101L XRE 3 pL1A1N 2 2 F 1 MEM L HepG2 65 P450 CYP1A1 2 FETAL BOVINE SERUM FBS 56 C 30 20 3 200 mM 0 4 100 mM 5 1 500 mL 2 25 mL 3 10 mL 4 5 mL 6 JIS KO0557 A4 A3
222. 5mL 25mL 10uL 40uL 50uL 250uL 1000uL 8 50mL 1 20x90mm 33mm 0 22 A 80 150 C 3 3 1 1 2 3 4 5 75cm22 1 0 5mL 1mL 10mL COs 5 37C
223. HRGC HRMS JIS K0311 JTS K0811 2 5 1 11 2 6 5 4 192 2 5 1 11 2 6 5
224. 0 05m 12 1pg ml 0 308 12 1 x 0 308x0 05 40 1 x 0 0044 Xx 1000 10 0 17 97 2 1 2 1 1 30 3 g zz og mL DMSO 12 mL mL VW mL Cpz
225. 40 mL DMSO 1 pgDRQ 1 DMSO 2nL 0 0494 TEQ DEQ 1 DMSO 2 4nL 2 nL x2 10 40 mL pr 1 pgDEQ Cpr 0 0033 ng TEQ m3N 0 1 30 0 0494 TEQ DEGQ Ve DMSO 40nL Vc 1 DMSO 2nL 23 50 mL Vs 25 mL _1x00494 1 2 AE 1000 0 0033 25 138 2 1
226. 2 2 4 3 8 ul 7 2 70 60 5 150C 2000psi 4 3 4 UL 4 192 2 H 208 HCI 2mol L 2 4 3 4 2 7 2
227. 8 6 9 3 4 90 C 10 90 3 4 DMSO 2 5 mL 2 5 mL min DMSO 0 9 mL 3 6 10 DMSO 90 gt DMSO 3 6 10 DMSO 147 5 1
228. 4 192 4 1 4 3 5 3 HRGC HRMS 94 6 n 25 n 20 HRGC HRMS HRGC HRMS
229. 1 2 8 7 8 TeCDD DMSO 0 DMSO 0 1 0 8 0 5 1 3 5 10 30 50 100 150 300pM well DMSO 110 2 4 1 5 1 12 5 RLU pM well CV 309 20 2 5 3 4 9 60 40 0 3pM well12 1pg ml 0 3 50pM well oO 20 12 1 2010pg ml
230. PBSC 100 pL 50 pL 10 5 was 69 5 5 1 1 STD RLU RLU fold induction 2 STD fold induction Y 3 2 8 140 120 100 2 PP 3 0 RR 6 SS 60 A 40 20 0 0 2 4 6 8 10 TeCDD ng m 83 2 8 5 2
231. 3 2 143 40 0 5mL 3 1 2 0 2 g 44 6 g 0 2 g 10 1 g 3g 8 6 5 3g 0 2g 0 2g 3 6 5 4 1 70 mL 1 2
232. 3 HBSS 5mL HBSS 4 3mL 5 3 6 7 10mL 8 10mL 9 8 COs 5 87 2 4 10 80 2 11 3 2 7 8
233. 1 8 JIS K8987 9 JIS K8180 10 JIS K8951 11 1 6 12 JIS K0811 6 2 13 2 JIS K0311 6 2 14 44 JIS K0311 6 2 15 10 JIS K0311 6 2 16 JIS K0811 6 2 17 JIS KO311 6 2 18 JIS KO1107 19 JIS K8855 20 JIS K8886 21
234. 1mL DMSO 0 011ng TEQ mL 0 018 10 017 4 8m3N EX 2 A 2 1 1 30 3 1 Ve W x x x Qpr Xv Vg 5 zz ng TEQ mL DMSO 161 vy mL Ve mL VE
235. 4 3 9 a 00 Tielil sw 0 0 Ne DTZ EEE EE ZT ET ETE ES 222 4 8 10 8 mN g 6 ml m ng m N ng g 7 10 20 1 0 05 13 3 0 35 100 10 1 00 10 20 0 07 20 _ 100 10 20 0 07 20 6 m3N g 7 ng m3N ng g 8 7
236. 71 10 ng mL 6 1 1 2 3 7 8 TeCDD DMSO DMSO 0 1 0 8 0 5 ng mL 2 1 1 n 5 2 1 RLU 1 3 2 4
237. E 4 GTD3 8TD6 DMSO 8 6 20 0s 20 CV 3 1 1 TCP 1 DMSO 4 ng m3N 30 H n 5 STD1 STD2 HH 20 TCP 4 TCP DMSO 4 1 5 4 1 5 STD1 STD2 STD3 STD4 STD5 STD6 ng7mL 125000 381250 7813 1953 488 122 0 2 ul 1 2 HI
238. 18 2 3 2 L 3 X2 12 pL 15 L 1 500 L 152 3 uL 15 000 pL 4 3 ELISA 1 4 1 3 E 1 9 200 nL HH
239. 2 1 JIS K0557 A4 A3 2 JIS K8891 3 JIS K8040 4 JISK8680 5 JIS K8117 6 JIS K8825 7 JIS K8361 8 9 JIS K8987 10 545 11 JIS K8180 12 JIS K8951 13 1 6 14
240. 3 1 H1L6 1c2 17 92 1 1 1 H1L6 1c2 83 1 1 83 1 1 2 1 Cell Line 2 DNA
241. 3 8 0 1 10 mL 2 3 4 4 1 118 4 2 1 JIS K0311 6 4 8 5 3 JIST HCL 2mol L 20mmol g 3 2 J 16 3 5 3
242. 1 1 2 4 5 2 1 6 1mL 2 DMSO 5 5 4 5 TCAP 1 0 ng7mL 40 pg mL 3 120 ng7mL 360 ng mL 5 1 100 ug mL 8 000 ng mL 6 3 000 ug mL 2 800 ng mL 190 3 4 mL 4 0 01 Triton
243. 1g 20 mmol HCI 2 mol L 16 4 2 4 187 4 3 4 2 5 DMSO 4 2 5 8 1 1 2 0 3 mL
244. CO 0 2 1 1 2 JIS K0311 5 3 1 3 JIS KO0311 1 JIS 1 5 2 JIS KO0311 IL TIII JIS K0811 5 2 1 4 JIS K0311 5 4 1 5 5 4 3
245. E RLU RLU 3 2 10 H 3 1 5 2 RLU Fold Induction 1 5 2 0 8 2 8 3 2 8 0 15 pg well 0 3 ng mL 0 25 pg well 0 5 ng mL 6 2
246. 2 90 10 3 90 3 DMSO 2 5mL 2 5mL min DMSO 1mL 3 5 9 4 DMSO 123 gt lt 90 DMSO 3 5 9 DMSO 5 1 DR EcoScreen
247. 4 1 2 iii a 4 1 2 162 2 1 JISK0557 A4 A3 3 JIS K8680 4 JIS K8117 2 JIS K8040 5 JIS K8825 6 DMSO JIS K9702 7 JIS K8987 8 JIS K8951 9
248. 450C 13 0 5 um 14 JIS K1107 2 1 DMSO 3 31 JIS R3503 JIS R3505 3 2 JIS R3503 3 3 KD 3 4 0 25 MPa
249. 2 8 3 gt ng TEQ mN gt pg TEQ D 3 1 2 3 5 6 7 2 8 3 A 4 5 3 1 1 3 2 2 2 1 2
250. 70 230nm 180C 48 1 32 30 1 15 38 3 14 100g 12 50 0g 16 XCARB XCARB 1 17 0 5nm 3 3 1 JIS R3503 JIS R3505
251. ECso pg TEQ nL 3 3 10 un 2o 90 1 0E 06 8 0E 05 6 0E 05 4 0E 05 2 0E 05 2 3 7 3 3 9 1 10 8 TeCDD pg U L 100 15 5 3 RLU 5 1 3 3 10 20 25 30 35 40
252. RE mi Wr O 2 DM wel ao ai as 1 y RLU x pM well ao RLU ai ECso a2 108 3 200000 160000 120000 RLU 80000 40000 0 1 1 10 100 2 3 7 8 TeCDD pM well 8 4 7 5 2 3pM well 1pM well 1 1000
253. 200 6X103 1 Y HRGC HRMS Y X Y X _ 26 3 x 103 x 0 5 20 1 200 6x103 10 0 17 0 77m3 2 1 182 2 1 1 30 3 X 1 1 Op k Vp Cp Ww M
254. 159 4 1 17 92 2 1 1 4 1 1 4 1 1 2 1
255. 3 60 8 1 24 60 8 PBS 50uL 8 4 6 96 6 60 8 PBS 50uL 50uL 3 4 6 5 6 30 5 5 1 1 3 DMSO g
256. 83 5 7 3 5 7 3 1 3 5 8 2 2 1 0g 5mL 3 60 10 122 4 60 85 mL 2 5 mL min 60C 3 5 8 4 1 3
257. E 18 000 pL 180 mL DMSO E 500nLX 18 7 5mLX4 30 000 pL pj 2 3 3 pj 200 uL E 98 18 3 x 200 E 1 500nL 18 000nL 28 500 pL 1 9 A 1 9 10 18 A 10 12 4 2 4 8 6 3 64 pg 32 pg 8 pg 4 pg 2 pg 1pg 1 200 nL C
258. 2 4 5 TCP I A 2 2 1 2 4 5 BS A 2 4 5 1 2 5 12
259. 6 1 CV 30 10 1 H HH 10 100pg mL 3 5 14 n 5 RLU RLU HH 3 5 2 DMSO RLU
260. 9 1 2 3 7 8 TeCDD 50ug mL Toluene 10 1 1 Conc TeCDD 5ug mL DMSO 9 DMSO 10 11 1 2 Gnternal TeCDD 250ng mL DMSO 10 1 1 DMSO 20 12 2 8 7 8 TeCDD DMSO 11 1 2 DMSO 8 1 1 3 su 39 13 2 PCDDs PCDFs 4111ng TEQ mL nonane 14 2 1 PCDDs PCDFs 180ng TREQ mL DMSO 13 DMSO DMSO 22 8 15 Quality Control PCDDs PCDFs QC 0 250ng TREQ mL DMSO 12 2 1 DMSO 720 3 1 2 16 17 Cell culture lysis reagent x5 solution Cell culture lysis reagent x5 solution 2mL 8mL 18 JIS KO0557 A4 A3 19 JIS K8825 20 COs gt 99 99 21
261. 96well Flat Bottom 25mm 0 2um 13mm 13x100mm 20uL 200uL 1000uL 8 2 4 1 170C 1 2 121 30 2 5 41 1 2
262. ng TEQ well uL well mL mL mL ng TEQ g 0 00085 0 0028 0 075 25 10 20 1 0 1 0 0 60 ng TEQ well uL we g mL mL mL ng TEQ g gt 0 00035 0 00056 0 075 25 50 0 10 20 1 0 1 0 0 12 7 1 1mL ng mL x Q a 08301 pV ng TEQ m3y 0 0669 x ss x 7 ng mL Ve mL VE mL vy mL
263. 2 2 70 60 5 150C 2000psi 119 2 4 192 3 5 4 HCI 2moII 20 mmol g 3 2 Y 16 3 5 4
264. 7 G418 GENETICIN G418 y 1 g 25 mL 40 mg mL 0 8 10000 units 10 mg mL 0 9 1xsolution 0 10 11 PBS 12 13 2 3 7 8 TeCDD 50 ng mL DMSO 14 COs99 5 15 16 1 2 DMSO 7 40 50 9 1 2 3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 100 mm 96
265. HH JIS 4 1 4 3 5 3 4 1 4 3 5 3 4 E 192 1 E yt 6 ug mL HRGC HRMS ng TEQ mL 4 4 16 n 35 20 jig TCPHA mYN 0 2 4 6 8 10 ng TEQ m N 4 4 16
266. 56 2 101L 17 92 1 2 1 101L 3 2 1 2 1 2 3 4 5 6 3 2 1 Ah Receptor Arylhydrocarbon Receptor
267. 21 i 17 92 2 3 j
268. 4 4 9 4 4 14 4 4 15 1 248 072 2 01es 0 168 0 1 0047 0031 0030 30031 0034 0029 0036 92lols9 181 4 4 9 n 6 SL lo 16 0 189 257 2373 3377 1204 1994 11933 2427 347 CV 0 000 0 001 4 4 14 001 0 1 uh g mL CV 0 0001 4 4 15 0 001 h g mL 0 01 0 1 1 0 0 0006ug mL B Bo 0 9599 B B 0 9 B B 0 9 0 0022ug mL
269. 1 20 15 3 2 ARNT extract 4 4 3 6 11 149 E AB1 AB2
270. 20 4 8 80C 9 4 4 1 1 2 3 4 5 6 7 150cm2 83 2 8 1 1 50mL 8 Q0mT 8 1 20mL 20uL 15uL X 100 1mL 18 7 5 105 cells mL
271. 13 CO gt 99 99 14 2 3 1 4100mm 2 96 3 10mL 25mL 4 0 22nm 5 200uL 1000uL 1200uL 85 6 7 8 96 9 10 8ch 12ch 11 12 37 5 COs 13 IB 14 15 16 17 80C
272. E 44 45 mL 189 4 2 6 E 0 3 0 01 g 10 mm HRGC HRMS
273. ng TRQ mL 4 3 4 a Pr oO 3 ng TEQ mL 218 4 ICso u 2c CV 20 4 8 4 4 3 13 5 1 77 2 1428 100 1i564 100 STD5 1 333 93 4 1425 91 1 STD4 1078 75 5 1235 79 0 STD3 0 668 46 8 102 0 61 0 834 STD2 0 254 17 8
274. 3 58 Op Vi 1 W X 1000 Vi C5 W pz pg mL DMSO 2 mL Ve QmL Vi mL Cpz ng TEQ g 4 3 ng TRQ g 0 1 ng TEQ g 10 mL 0 2 mL E
275. 4 8 2 4 3 12 4 3 3 x ng mL 4 0 B Bo B 1Cso C ICso ng mL 217 4 3 2 0 1 1 10 100 77 2 ng ml 4 8 12 4 3 3 4 4 0 B B 99 83 6 C ICso ng ml 0 740 D B B 4 702 5 2 1 ICso B Bo 50 4 4 8 2 STD3 B7B 50 2
276. 650nm 4 4 12 a a CX 0 B Bo a B Bo X ug TCPHA mL ICso ug TCPHA mL SR b Lg mL B B 1 0 0005 0 970 0 005 0 845 0 0095 0 745 08 0 02 0 603 0 05 0 411 a 0 2 0 178 1 0 052 0 4 4 a B Bo 0 997 02 b 0 878 c 1Cso 0 0326 0 0001 0 001 0 01 0 1 1 10 A ng ml 4 4 12 5 2 eG RE i pe nn u
277. 8 9 tt 3 1 2 3 75cm2 1 8 2 C 150cm2 25cm2 3 8 H 1 40 50 42 COs
278. 9 x Cs On ng m3N Os Cs ng m3N 4 20 0s 20 6 6 1 2 3 CV 30 20 2 3 3 3 10
279. E 2 1 4 6 2 2 4 3 1 5 1 2 3 4 5 6
280. 1 a 86 COs 5 37C EX 3 2 37 RR EDTA 1 2mL EDTA PBSC PBSC me 10 Bsd 10
281. 2 3 4 5 6 B B 0 100 7 IC 50 50 Inhibition Concentration 8 CV N gt H 160 3 1
282. 20mL 10mL 0 522 1mL 4 1 4 3 2 4 8 2 2 1 JISK0557 A4 A3 2 JIS K8891 3 JIS K8040 4 JISK8680
283. 80 3 5 2 4 4 1 1 3 2 9 200uL 2 2 3 4 4 2 1 4 4 3 DMSO 2 100 3 2 1 H STD 0 5
284. Ah ARNT Ah 2 8 7 8 TeCDD 1 2 a mm 2 1 AR NT
285. MS 3 3 KD 3 4 JIS K0811 4 192 H 1H 1 4 mm 1 pH pH 2 3 033 mm ASE 4 60 mL 5 10 mL
286. ng TEQ mL ng mL x QC ng TEQ mL 0 53 pg uL x0 642 0 35 QC 8 1 6 3 1 6 STD5 TeCDD 0 78ppt QC QC ng TEQ mL 0 53 g uL x0 642 0 35 6 3 STD5 TeCDD RLU 00 ur 0 78ppt gmL pg 400uL pg TEQ uL 7815 6 64 7 68 3 07 1 100 4 0 77 C RLU L 9 DD 400D 9 MD eTEQLL 5923 6 28 5 34 2 14 1 100 4 0 53 QC PCDDs PCDFs TeCDD 0 78ppt 0 50 1 00 3 3 E 3 E 0 75 i 5 20 0 10 T T T T 0 50 T T T T 0 10 20 30 40 50 0 10 20 30 40 50 8 1 11 QC 8 1 12 TeCDD 0 78ppt 5 3
287. pH pH pH 7 HCD pH 7 2 3 ASE 100 2 1 10 60 vol 60 150 2000 psi 142 38 mL 4 a ASE 5mm b c ASE
288. 1 36 6 4 100uL 3 60 5 6 1 2 3 4 5 4 2 1 DMSO DMSO 3 12 3 4 1 2 3 4 5 1 2 3 48 500u DMSO 8ul 3 4 2 16 24 60 100uL 38 7
289. 2 4 1 4 3 5 3 134 HT aE 6 n 31 n 20 HRGC HRMS HRGC HRMS 1 2 Y X X HRGC HRMS 0 2057 HRGC HRMS ng TEQ m N y 0 2
290. 20 30 Bsd BE 15 000 cells 100uL 96 fF DMSO TEGQ 1 10 100 1000 4 3 3 1 8 3 1 uL DMSO uL 1 0 10 10 90 100 10 10 90 1000 100 10 90 1 0 DMSO 0 1 0 2 0 4 0 6 0 8 1 0 2 0 3 0 4 0 5 0 10 20
291. C xC 0 On ng m3N Os 5 Cs ng m3N 5 20 Os 20 6 6 1 CV 30 20 2 n 6 1 1 100ng mL STD1 DMSO 4 STD2 2 STD2 DMSO 4 STD3 3 4 STD4 STD7 H 7 4 3 6
292. ul 220 5 1 A DMSO DMSO DMSO DMSO DMSO DMSO 1 C TD 0 STD STD STD 1 6 3 6 4 6 5 D 0 STD SID STD 1 5 3 5 4 5 5 E TD 1 STD STD STD 1 4 3 4 4 4 5 F T STD STD 1 3 3 3 4 C 1 9 STD STD 2 3 2 4 H STD STD STD STD 1 2 1 3 1 4 1 5 4 3 14 96 3 O i 1 oo go 3 1 4 3 7 DO eb Pl 2 ll EC 91eoelO1OImIOIO COWIOm 6 7 STD3 6 25 7 696 16 747 6 889 7 975 6 544 6 889 7 123 0 573 8 0 CV
293. 17 17 92 2 2 17 92 2 3 E F 92 2 4
294. 3 5 3 6 0 25MPa 141 4 4 1 4 2 1 JIS K0311 6 4 2 4 192 3 6 3 ASE pH 7 3 6 8 1
295. 4 3 1 20mL DMSO 20 1000pg TEQ mL Ve C Xx SKU000 WV mL 165 C DMSO 20 1000pg TEQ mL DMSO mL mL ng TRQ g ng TEQ m3N X g m3 N 1ng TEQ g 5g 250pg TEQ mL 1mL 20mL
296. D2 94 81 2 E F G H 1 3 B A 0 8 1 2 6 26 D C 63 3 6 15 157 6 6 1 CV 20 1 3 5 5 1 6 2 7
297. E a GE 9 150cm2 3 2 1 150cm2 100 3 1 3 8 2 3 10mL PBSC 5mL PBS 4 2mL 8 5
298. 1 2 4 5 TCP 1mL 2 DMSO 10mL 3 15mL 168 4 10 50mL 5 1 6 10mL 7 15mL 8 0 15mL 9 15mL 10 15mL 11 1 12 2 2 1 JISK0557 A4 A3 2 3 1 450 nm 2 3 4 5 10 100uL 6 100 1000uL 7 1 10mL
299. 20 Os 20 2 Cw X7 g 2 V s WW Cy ng g X ng mL 12 mL Vm mL mL g 6 6 1 CV 30 20 2 6 1 92 1 2 3 7 8 TeCDD
300. 3 22 O 2 2 4 g 1
301. 3 3 8 ul 2 3 3 4 81 Me 3 3 4 1 2g 2 10mL 10mL 10
302. 4 4 3 20 24 96 96 PBSC 80 100uL well 96 PBSC PBSC 80 100uL well PBSC 5 5 96
303. 5 7 5 8 NN 30 2 5 9 6 1
304. CV 30 20 2 1 3 1 8 3 3 1 8 STDO STD1 STD2 STD3 STD4 STD5 STD6 STD7 STD8 STD9 STD10 2 3 7 8 TeCDD 25 0 12 5 6 25 3 13 1 56 0 781 0 391 0 195 0 0997 0 0488 0 0244 2 mL n 5 CV
305. JIS K3800 FH 5 6 E
306. en 3 9 x 7 MSO 3 1 1 Qg mL 3 STDO STD1 STD2 STD3 8TD4 STD5 STD6 STD7 STD8 STD9 2 3 7 8 TeCDD 25 0 12 5 6 25 3 13 1 56 0 781 0 391 0 195 0 0997 0 0488 8 1 2 QC ng mL EE ts Go 2 3 7 8 TeCDD 12 8 7 PeCDD Son Sn lo 1 2 8 4 7 8 HxCDD 1 2 9 6 7 8 HxCDD 1 2 8 7 8 9 HxCDD 2008 88 0 _1 2 3 4 6 7 8 HpCDD A CC CR EY ial OCDD 5000 219 6 30 2 7 8 TeCDE 1 2 9 7 8 PeCDF 1000 44 0 061 3478PeCDR 1 2 9 4 7 8 HxCDE 1 2 9 6 7 8 HxCDF 1 2 9 7 8 9 HxCDF 2 3 4 6 7 8 HxCDF 2000 88 0 12 1 2 3 4 6 7 8 HpCDF 1 2 3 4 7 8 9 HpCDF 2 EE OCDE 5000 219 6 30 Total PCDD Fsng TBGmD 3893 171 0 237 2 3 40
307. ng TEQ g 4 3ng TEQ g 1 0 1ng TEQ g 0 05mL J _ 12 1 x 0 356 x 0 05 4 1 90mL 30mL 12 1pg m1 0 356 1000 a 0 0065 98 4 1 8 4 2
308. 3 7 6 2 2 Mi 200 gL 7 64 pg 32 pg 16 pg 8 pg 4 pg 2 pg 1 pg 0 pg DMSO 6 64 pg 32 pg 16 pg 8 pg 4 pg 2 pg Opg DMSO 3 CV 20 173 1 4pg 0 3 1pg 3 6 14 4 1 3 CV 9 154 Y miX m X mX b Y pgDEQ XX mOD mi ms ms b R Y pg 4 5866E 09 1 0961E 05 pgDEQ 70 60 50 40 1 a Le 500 1 000 1 500 2 000 2 500 OD x1000 405nm 8 6 14
309. 2 2 8 1 1 15 25C 1 2 8 7 2 1 1 2 DMSO DMSO 18 5C 20 25 C 15 25 1 15 25 30 3 15 25 30 4 1 1
310. 4 1 7 ng TEQ well ng TEQ mL 0 00028 0 011 0 00023 0 009 4 1 8 gt TBGweD GTEG mi 0 00043 0 055 0 017 2 2 0 00085 0 075 0 014 3 0 6 1 4 1 9 4 1 10 4 1 9 Qg TEQ wel uLwe GnsN mm TL GgTgem 0 00028 25 4 0 12 0 10 20 1 0 1 0 0 0055
311. B Bo 0 9 CV 20 B B 0 85 B B 0 85 CV 1 4 4 8 5 1 2 4 4 8 STD1 STD2 STD3 STD4 STDS STD6 STD7 STDs TCPHA pg m7 0 001 01 019 04 1 4 20 40 us m 0 0 0005 0 00510 0095 002 005 02 1 2 2 1 n 5 CV
312. 2 0 4 mL 3 2 1 2 8 g 44 10 4 g 0 2 g 20 3 6 g 1 5 g _ 3 6 7 145 1 5g 20 3 6 g 0 2g 44 10 4 g 2 3 g
313. 17 92 2 2 1 f 4 2 1 4 2 1 2 1 2204
314. 3 4 4 1 2 3 24 4 5 4 5 1 2 3
315. 2 1 1 80 3 Op Xk 1 Vc VE x x x 1000 Cp Ve Me Ww Y WW zz pgDRQ Cpr ng TEQ g Ve DMSO uL Ve 1 DMSO nL Ve GmL Ve ml 4 1 ng TEQ g
316. 24 1 5 25 125 625 pL DMSO pL 1 1 a 0 5 1 20 80 25 5 20 80 125 25 20 80 625 125 20 80 127 STD 100 STD 200 STD 300 STD 500 STD1000 7 7 7 B x625 B x125 B x25 3 5 10 96 7 PBS 0 1mL 4 3 1 HH
317. JIS R3503 76 3 5 50 38kHz 3 6 79 3 2 3 4 4 4 1 4 2 1 JIS KO0811 6 4 3 8 8 80 HCI 2mol 7 1g 20mmol 16 2 3 gn
318. gt 4 1 1 ELISA 1 4 2 1 8 2 2 7 6 2 3 6 11 30 C 2 AB1 30C 1 AB2 30 C 1 30 C 30 60 3 1 200uL 64pg 32pg 16pg 8pg 4pg 2pg 1 pg 0 pg 7 64pg 32pg 16pg 8pg 4pg 2pg Opg 6 3 3 4 ELISA 8 6 12 2 7 2 3
319. 12 8 4 3 5 13 25 14 a 3 3 1 3 2 lt 2 G 2 1000 rpm 5 3 2 0 X106 4 0X 106 cells mL 2 4 1 mL 2
320. 4 1 20 4 2 20 25C DMSO H 4 3 4 4 DMSO DMSO TEQ 192 1 8 9 27 81 5 4 2 1 4
321. Ah 2 3 7 8 TeCDD 2 1 4 E DR EcoScreen E XRE 7 1 pIND GCDR7 Hepa 1c1c7 P450 CYP1A1 1 8 Takeuchi et al In vitro screening for aryl hydrocarbon receptor agonistic activity in 200 pesticides using a highly sensitive reporter cell hne DR EcoScreen cells and in vivo mouse Iiver cytochrome P450 1A
322. LL 3 3 1 1 2 3 4 5 6 3 2 1 2 3 4 5 6 7 101L 12 mL 5 3 1 37 2 G418 120uL 100 units 0 1 mg mL 3 4 1
323. 2 3 1 50uL 3 1 5 1 1 40 50uL 1 400 5uL 1 4000 0 5uL 2 QC a b 9 183mm 3 NC a 138mm 2 DMSO 1 DMSO 4uL QC 4uL DMSO 4nL 0 5mL 1 40 1mL 22 f 2 3 4 5 QC NC E
324. Ah 8 C Ah 9 Ah 2 Hill Hill Michaelis Menten V Vnax X b K S SE Vmax V 1 2Vmax b Intercept parameter 8S LS r 3
325. 1 150g K0811 6 2
326. 1 6 3 1 6 DMSO DMSO 18 5 20 25C 13 mmew X 100 mm 300uL 4 4 2 7 1 6 1 3 6 18 3 1 8
327. 1000uL 2 4 4 3 1 2800uL DMSO 4 4 8 1 0 022m3N DMSO 200uL 3 1 2 DMSO 200 X pL 200 2X uL Xul 2XuL 2800uL 2800uL 1000uL 1000uL 4000uL 4000uL N 89mg 4 4 4 X 80pL H
328. 4 RLU 3 On ngm3N Os 4 Cs ng m3N 4 209 Os 20 130 6 6 1 2 8 20 2 3
329. 5 1 M S Denison et al Bioassay for detecting 2 3 7 8 tetrachlorodibenzo para dioxin and TCDD like compounds and novel recombinant cell line useful therefor US Patent 5 854 010 1998 2 Dalhp Han Scott R Nagy and Michael S Denison Comparison of recombinant cell bioassays for the detection of Ah receptor agonists BioFactors 20 2004 11 22 2 2 1 RPMI1640 FBS 8 1 2 500mL 3 5mL 44mL 2 RPMTI1640 with L Glutamine 3 5000units mL 5mg mL 0C 4 Tetal Bovine Serum FBS 56C 380 20 C 5 0 25 0 6 PBS 7 DMSO JIS K9702 7 8 34mL DMSO 6mL 0 2nm
330. DRE AR NT 1 1 Cytosol 7 5 mLx4 2 ARNT ARNT Extract 250uLx2 3 DREF 60uLx2 4 Activator 0 75 mLx2 5 AB1 lt 120 pL 6 AB 25 mLx2 7 ELISA 25 mL 8 83 3 mL 9 4 10 ELISA 1 11 v oNAP 200 nuL 0 5 mg mL DMSO 12 AB2 lt 120 pL 13 5 14 1 15 1 1 F C 2 80 4 2 2
331. NaN K9501 10 JIS K8101 11 1000mL 2 79g 7 60g 0 20g 0 20g 0 20g 1 0g 0 45hm 12 800mL 2 79g 7 60g 0 20g 0 20g 0 20g 1 0g DMSO 50mL 1000mL 0 45hm 13 DMSO JIS K9702 14 95mL 0 10g 5mL DMSO 2 3
332. 19 8 1 7 1 RLU Hill 6 1 un x Cd RLU b 2 pg mL LL 6 ek 100 X pg mL 3 pg 400pL 50 7 og 400uL X pg mL 4 og 4mL Cc pg 4mL 5 pg Cg og pg 4mL Vz CmD Vz mL 6 ng m3N ng g C 1 Cs x 1000 Cs ng m3N ng g V
333. 3 1 9 5 CO 5 37 20 24 3 1 9 B2 G2 B3 G8 B4 G4 B5 G5 QC NC 2 1 2 2 4 3 1 1 20 24 2 3 8 PBS 50uL 4 PBS 5 6
334. 7 DMSO JIS K9702 8 JIS K8987 9 JIS K8951 10 HH 140 3 6 2 8 6 1 P CB 3g 10 1g PCB 0 2g 44 6g P CB 0 2g 11
335. Y X 26 3 x 103x0 5 10 U 1755x103 10 0 1 9 183 4 1 4 2 2 2 1 JISK0557 A4 A3 2 JISK8040 3 JIS K8680 4 JISK8117 5 JIS K8825
336. 2 3 4 192 1 2 4 3 4 Qpi XVXk Vi 1 WwW 1090 x x CC WW g zz pg TEQ mL 12 mL Vg mL mL Czz ng TRQ g 3 4
337. 8 30 4 2 1 1 2 3 7 8 TeCDD DMSO 32 ng mL DMSO 1 3 12 pL 2 pL x 2 x3 12 pL 151 2 1000 mL 500 mL 3 AB1 AB1 AB 1 AB 2
338. a z i i 4 3 6 ng ml 100 25 6 25 1 56 0 39110 097710 0244 0 2 1 2 5 n 6 7 4 3 14 2 4 A D 3 CV 4 3 7 4 4 3 15 5 80 20 2 3 5
339. DMSO 4uL 2 DMSO 1 18mm 1mL 13mm 12 2 46 3 2 4 2 5 400uL 10 3 1 14 24 2 8 3 4 2 DMSO QC NC 190uL 2 8
340. m3N 2 1mL ng mL x Q 0 0 9605 v Ve ee mg TEQ g 0 0601 x mm ES Vz mL mL mL ng mL 178 2 2 8 6 1 HRGC HRMS HRGC HRMS
341. 0 5nhm 1 DMSO 3 3 1 JIS R3503 JTS R3505 3 2 JIS R3503 3 3 KD 3 4 8 2 JIS R3503 71
342. 2800uL 1000uL 4 4 1 2800uL 4 4 1 DMSO H DMSO 200uL 1000uL 4 4 1 1 Bo DMSO 400uL OuL 200 X uL TCPHA 8 200uL Xul
343. 3 4 14mm 8300mm 3 5 6mm 50mm 3 6 100 3 7 0 1 10 mL 2 4 4 1 4 2 1 JIS K0311 6 4 4 8 8 207 HCI 2mol L 20 mmol g 3 2
344. 4 4 11 Fr Rt Wr 3 1 4 2 1 4 2 2 4 2 4 2 4 4 11 4 1 4 2 1 4 2 2 4 2 5 2 4 4 11 5 1 4 2 1 4 2 2 4 2 6 2 4 4 11
345. CV CV 5 2 1 1 0 5 2 64 pg 1 0 20 60 2
346. DMSO mL 0 04 mL 40 uL vs 1 DMSO mL 0 002 mL 2 pL Vs mL Vs mL g W 3 4 0 8 1 2 0 8 1 2 8 6 15 MD 2 3 22 Bl 52139 86 606 0 8 1 2 C 51121 84332 13 701 2 2
347. NN 228 TCPHA A 1 x 2 4 o 1 2 1 1 30 3 Qp Xxnxv WV 1 Y DL 6 V m3N pz ug mL
348. 4 4 5 7 TCPHA 200uL 2800uL 1000uL TCPHA 20 TCPHA DMSO 4 4 5 STD2 4 0O 00005 0005 00095 002 005 02 1 3 TCPHA 4 a d 245 TCPHA
349. 7 1g 1 7g 8 1 6 b 50mL 3 2 a 3 6 0 5g 1 XCARB 0 8g 3 1 0g 3 1 7 b 5mL 20mL 10mL 2 HRGC HRMS 3 1 XCARB 3cm 3cm 3cm 0 5g XCARB K19 0 3g 3cm 1 0g amp 8 1 7
350. ODMSO 0 1 0 1 1 10 100 1000 STD pM well 3 4 9 6 2 7 TrT 3 4 10 HRGC HRMS y 0 808x y 0 356x ng g ngm3N
351. uL DMSO uL 1 1 0 5 1 20 80 25 5 20 80 125 25 20 80 625 125 20 80 4 3 1 2 3 4 5 6 mi E E 1 2 1 RLU 3 3 3 2 7 96 3
352. 3 1 2 3 gt 4 12 2 END ONE RN 400uL 10 QC NC 14 24 o 2 DMSO H 45 QC 1NC 5 37C COs 5 20 24 190uL 1 QC NC 190uL 2
353. 4 4 1 4 2 gt E 1 1 2 23 2 1 2 3 1 2 3 4 1 4 3 4 4 4 7
354. 4 2 10 4 2 3 1 2 ug TCAP mL 21 0 SNS On ng m3N Os 3 Cs ngm3N 8 20 Os 20 2 Cy 6 6 1 20 2 2 CV
355. 50mL 6 CD 1 7 238 2 2 HH 1 1 JIS K0557 A4 A3 2 JIS K8001 3 JIS K8001 4 JIS K8001 5 JIS K8001 6 PBS JIS KO0461 7 BSA JIS L1902 8 NaOH JIS K8576 9
356. 6 DMSO 7 JIS K8987 4 2 2 HH HD 8 9 JIS K8951 11 12 4 JIS K9702 10 JIS K0311 E HH 184 3 Bn WO E 1
357. 6 9mm 7 2 5 4 mm 8 50mL 9 KD 12 mmxL 92mm DMSO 60 4 4 1 4 2 1 JIS K0311 6 4 8 2 3 8 HCI 2mol 7 1 g 20 mmol 16 3 gn 3 2 8 61 2 2 4 192
358. A4 A3 2 DMSO JIS K9702 1 1 2 3 DMSO 1 2 3 4 5 6 7 8 9 10 11 12 10 100uL 100 1 000LL 13 mm X 100 mm 1 000 200 200 0 001 g 200 9 191 3 1 2 8 15 25
359. HeB5 17 92 1 3 76 1 76 SENNN SI NS 5 76 3 2 76 4 78 SS 85 6 NN Ni Sl 95 4 H4 TE Iuc 17 92 1 4 96 dhI 259 MSDOS02090SRR02UO2RMD0D02A083308 96 2 0 os 96 3 21222222 SS2 St 229 97 SGS SR2 0 99 S A 104 6 Nk SR ER 113 5 DR EcoScreen
360. ng TEQ m3N 4 20 mL lt 10 mL 0 08 mL _ 300 x 0 185 1000 300pg mL US 20 1 0 185 0 052 10 0 17 2 a 1 2 1 1 30
361. 150C 80 8 2 3 5 2 3 9 8 1 CO2 5 37 10 1 2 3 4 5 8 4 4 1 1 106 3 2 1 6 2 50mL 5 10 mL 3 PBS C HBSS 200HL
362. 17 92 2 3 1 4 3 1 Rue NR 4 3 1 2 1 B
363. 2 7 2 70 60 5 150C 2000psi 2 4 192 4 4 4 2 a 233 HCI 2moD 20 mmol g 4 4 4 4 3 2 3 3 2 7 2 70 60 5 150C 2000psi 4 4 5
364. 3 1 4 3 8 2 2 5mL 3 60 10 211 4 60 85 mL 2 5 mL min 60C 4 8 8 4 1 1 2 90 10 3 90
365. 5 6 5 B 5 5 1 TCP 1 2 4 5 TCP 2 4 1 2 TOCP DMSO 5 STD1 STD1 DMSO 4 STD2 4
366. 5 5 5 11 5 1 1 CV 80 20 3 4 6 1 2
367. 6 3 84 4 77 2 CO Et 2 2 1 6 3 3 4 4 1 96well 1 2 0 03759 Triton X 100 0 05 72N 7mL 3 3 5mL 2 4 7mL 2 5
368. HH ngmL 91 RLU RLU RLU 107G 9 1 Cc XxXnxvX 2 Vg VY Cs ng m3N X ng mL 2 mL Vz mL mL m3N 9 21 0 C On ng m3N Os 2 Cs ng m3N C x Cs 2
369. Vz GmT mL Czz ng TEQ m3N 4 0 30 5ng TEQ m3N 0 17ng TEQ m3N 50mL 10mL 4mL 0 977pg ml1 0 221 _ 0 977x0 4x0 221x4 50_ 1 1000 YO 2 1
370. mL Cpz ng TEQ g 4 3ng TEQ g 0 1ng TEQ g 20mL 10mL 1mL DMSO 0 009ng TEQ mL WwW 0 009 x 1 a 8 U x 907 4 1
371. 18 JIS K1107 1 19 JTS K8251 3 3 1 JIS R3503 JIS R3505 3 2 ASE 200 ASE 3 3 KD 3 4 14mm 300mm 3 5 6mm
372. 3 1 37 2 3 4 5 6 7 8 9 10 9 PCBs PCDDs PCDFs 2mL 2mL 1mL 10mL 10mL PCBs 10 10 80 15mL PCBs 20mL PCDDs PCDFs
373. 4 5 6 4 6 2o n g oc CV 20 1
374. 4 2 300 500 1000 pg mL 1 2 10 24 STD 3 5 10 E 6 4 4 8 3 5 1 1 2 8 7 8 TeCDD STD DMSO 10 20 30 50 100 200 10nL 3 1 STD 6
375. TCAP 2 2 1 2 4 5 TCAP 1 100 20
376. TCP 0 05ug mL 4 3 DMSO DMSO 8 n 2 1 8 H 1 TCP 80uL 1 4 8 2 DMSO 80uL 80uL 7 H DMSO 80uL 2 2 4 4 a TCP b 2 4 1 1 1 1 160 80uL 3 8 hh 80uL 8 L 80uL 3
377. 2 Y X 3 3 12 Y X 0 390 n 44 WHO TEF 2006 1000 Se 100 5 E Ra F 10 1 a 3 001 G 001 001 01 1 10 100 1000 0 01 0 1 1 10 100 1000 ng m3N ng g 3 3 12 H HH 95 4 H4TE Iuc 17 92 1 4 1 H4 IE 1luc 8 4 1
378. H E Lg mL Hg m HH m3N g 8 ugm3N ug g H H 3 4 4 12 aa WF Fr vz y E 0 SN or L mL ml m3N g 10 nug m3N pg g 250 7 4 4 16 HRGC HRMS y 1 488x y 1 221x y 1 267x ng m3N ng g
379. JIS K0311 6 2 12 44 JIS K0311 6 2 13 JIS KO311 6 2 14 JIS KO1107 DMSO nl 3 Bn ud 9 x 99 3 3 1 JTS R3503 JIS R3505 3 2 JIS R3503 3 3
380. 1 2 3 7 8 TeCDD 32 ng mL DMSO 2 148 2 2 3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 3 405 nm 80 C 4 C 30 96 200 400 pL 12 2 10 pL 1000 mL 1000 mLX3 5L 5L 2 nuL 1 200 uL 1 7 1 mL 2 2 2 1
381. 5 JIS K8117 6 JISK8825 7 8 DMSO JIS K9702 9 JIS K8180 10 JISK8951 11 JISK8550 12 JIS K8987 13 1 6 14 JIS K0311 6 2 15 44 JIS K0311 6 2 16 20 14 100 g 11 625 g L 40 mL 206
382. Hill MM ST Ci b VV RLU Vaax U0 S Kz V1 2 Vmax 48 Hills coefficient Hill Slope parameter b Intercept parameter 4 2 3 7 8 TeCDD RLU 10 RLU A Wu i 4 zagx 7 RLU 2 pg
383. 4 4 10 240 DXN 4 4 10 241 4 2 1 B DMSO 400uL 5600uL 2000pL 2 200uL 3 5
384. 1 H1L6 1c2 Ah 2 3 7 8 TeCDD 2 2 1 H1L6 1c2 HH DRE 4 P450 CYP1A1 pGudLuc6 1 Hepa 1c1c7 5
385. 15 44 JIS K0311 6 2 16 20 14 100 g 11 625 g L 40 mL 17 JIS K0311 6 2 18 JIS K1107 1 19 JIS K8251 20 JIS KO0311 6 2 1 DMSO 3
386. 2 1 1 30 3 Ox0 4xKxv Vi 1 MW 1000 Vs 6 pz og mL 6 1 2 mL Vg mT mL Cpz ng TEQ g 4
387. 2 2 4 4 4 4 4 4 4 3 6 12 ELISA 8 8 8 8 8 8 8 2 3 2 4 8 10 11 2 2 4 4 8 8 2 ELISA 2 4 8 12 13 2 2 4 4 4 8 8 8 8 3 6 13 ELISA 2 2 2 4 4 8 8 15 16 17 18 2 2 2 2 4 4 4 1 4 8
388. K0311 6 3 5mm 2mm 20g 100g g 50mL 100mL 105 110 2 30 Bg B Ax100 20g 100g
389. 1 RLU 15 STD8 0 977 8TD 31 2 pg mL 6 1 RLU 4 2 2 1 1 8 b 1 4 13mm DMSO 4uL 4mL 16 16 2 QC 8 3 a 9 NC H QC 4uL 13mm
390. 1 1mL ng mL x Q ogrTpQmsN 0x0 422 xx 0 ng mL Vz GmL Vg mL mL Gm3N 2 1mL ng mL x Q V mg TEQ g x0 595 xx iM ng m1 Ve mL Vz m1 vy mL 1mL ng mL x Q
391. 200uL 1000nL Hm 87C 4 59 1mL 0 5mL CO2 5 37C 3 1 E 105 6 1 3 3 3 2 1 HBSS 87C 2
392. 247 Vg m1 m3N g 5 0 0095 sg TCPHA ug mL ug mL Ss S Nk pia 9 5 O ng m3N Os 6 Cs ng m3N 6 20 O 20 6 6 1 CV 30 20 2 CV 30 B B 0 9
393. 70 60 5 150 C 2000psi 4 3 4 8 5 209 60 90 DMSO 4 3 5 im 1 1 1 0m3N 1 0g 1 2 0 4 mL
394. Ve 1 V s W Cw ug TCAP g ug TCAP mL 12 mL Ve mL Ve mL Ww 80 n 10 1 1 1 2 1 2 4 2 4 4 2 4 A SS IT 0 DS 3 5 6 ug mL 0 0 13 0 20 8 28 1 42 9 126 3 377 8 1 116 6 3 318 6 2 1 6 2 lt
395. 0 0009ug mL B B 0 945 B Bo 0 85 4 4 10 H ug mL B Bo 0 85 0 0038uug7mL 0 0022 249 TCPHA 6 1 6 2 TCPHA
396. 3 8 3 3 2 2 10 10 ng mL 3 2 2 Nt DMSO RLU 195 244 202 224 196 21 3 932 RLU 982xTeCDD R2 0 990 21 3 932x3 3 0 075 ng mL 21 3 932x10 0 23 ng mL 500 400 300 NM 0 ji Mb UM 4 a 200 100 0 0 0 0 1 0 2 0 3 0 4 0 5 TeCDD ng m 8 2 10 3 72 E
397. 3 DNA DNA 4 Exogenous Gene 5 Luciferase Gene 6 DRE Dioxin Responsive Element 7 Vector DNA DNA 29 8 Ligand 9 Ah Arylhydrocarbon Receptor
398. 92 1 3 nl 7 d 20 1 E HeB5 o E
399. COs 5 87 24 COs 5 37C 16 24 E 7 8 4 1 3 4 6 1 uL DMSO uL 50 0 2 25 25 6 2 15 30 20 2 10 90 60 6 10 90 3 4 2 pM well nM pg mL 0 3 0 0375 12 075 1 0 125 40 25 3 0 875 120 75 10 1 25 402 5 30 3 75 1207 5 100 12 5 4025 300 37 5 12075 107 4 3 1 PBS CaCl MgC1 PBSC CaC1 MgC1 8 1 1 8 8 PBS 2 2 3 4
400. 0 129 16 0 543 43 4 6 975 RR 1 10 16 100 4 3 10 4 2 DMSO 20 5 1 0 8 0 1ng mL 4 3 4 4 24mL 3 5mL 4 5 well 40L well 2 3 0 100 DMSO 10uL well 20 DMSO 4 50uL well 2 18 25 C 90 3 n 3 4 3 11
401. 200 nL 200nL 200nL 200nL E 200nL 200 pL CO 200 mL 4 5 O 4 3 1 G 200nL
402. 225 ns 20 0 5952x R 0 8869 ng TEQ m1 ng m1 4 8 17 ng mL HRGC HRMS ng TEQ m1 4 3 18 oO n 20 y 0 5221x RS 0 8836 ng TEQ ml HF ng ml 4 8 18 226 4 17 92 2 4 1
403. 5600uL 2800uL 2800uL 2000uL 1000uL 1000uL 8000uL 4000uL 4000uL X 0 022m3N 44mg 1m3NO 9mL 1g70 9mL 4 4 4 2 4 4 2 DMSO 2800uL DMSO X 20uL 40uL 200uL
404. EF ngmsN uL well mL mL mL 0 25 0 5 4 12 10 20 0 08 1 0 02 73 8 2 7 VOL oe well E 3 uL well mL mL mL 5 0 25 0 5 5 100 10 10 0 2 1 0 02 7 5 3 2 2 8 6 1 HRGC HRMS HRGC HRMS
405. 0 5 10 15 20 25 30 3 2 9 5 3 1 fold induction 2 fold induction 3 fold induction 4 5 fold induction rd 4 1 1
406. 1ogx RLU y y wi a B y 86 y Q 1 B EXP y LOG x 6 a 946789 21 B 15662918 vy 3 1685912 6 801 9887 x y 2 37E 0 8 8 8 5 2 3 3 9 50 RLU
407. B Bo TEO o B Bo TEQ Qg mL Qg TEQ mL Qg mL ng TEQ mL 1 929 100 1 810 100 STD7 1 787 92 6 1 796 99 2 STD6 1 587 82 3 1 584 87 5 STD5 1 138 59 0 196227 5 4 1 136 62 8 gt 197126 5 4 STD4 0 507 26 3 0 551 30 4 STD3 0 147 7 6 0 147 8 1 STD2 0 032 1 7 0 033 1 8 STD1 0 018 0 9 0 014 0 8 4 1 4 mg TEQ mL 5 49 9 72 ng TEQ mL o 0 3219 0 4837 C V 5 86 4 98 ng TEQ mL ip 2o 4 85 6 13 8 75 10 7 20 0 70 ng TEQ m1 ICs 4 1 9 5 3 B Bo 20 80
408. BE 3 FE 1 6 1 1 6 2 E
409. HRGC HRMS HRGC HRMS 5 10 HRGC HRMS 3 27
410. a H1L6 1c2 17 92 1 1 b H 101L 17 92 1 1 nl 7 c
411. 11 37 5 CO gt 12 13 100xg 14 15 16 17 18 612x75mm 19 20 21 22 80 23 125 3 3 1 SH F 1 Hepa1clc7 37 2 15mL
412. 2 1 15 mm 4 2 6 1 5 g 1 5 g 44 7 5 g 2 5 g 2 5 g 188 2 2 2 6 mm 10 mm 3 1 JIS KO0811 6 4 4 2 a b C 9 6 1 9 1 mL 1 mL 1 mL
413. 3 2 5 63 1 11 1 10 2 3 2 6 1 60 mL 44 10 0 1 g 1 8 0 1 g 5 5 5 g 2 12 mL 0 50 02 g 0 8 0 02 g 60 m lt 5 5 5 g 1 8 0 1 g 44 10 0 1
414. 4 AB2 AB2 AB 1 AB1 AB 2 5 4 15 AB2 2 1 ARNT DRE 2 DRE 50 mL 1 DRE DRE
415. 4 1 CV 20 gt o 3 E 1 173 4 i LL mg TEQ mL 2o 4 1 4 tt 4 1 3 TCP n 1 n 2 B Bo TEQ
416. Arylhydrocarbon Receptor 2 Luciferase Gene 3 XRE Xenobiotics Responsive Element 4 Subculture 5 6 m ST KN 5 3 CV 3 76 1
417. DMSO 8 CaCl 2H O 0 13g 13 100mL 9 MgCL 6H O 0 1g 13 100mL 10 1 DMSO 4 1 11 2 3 7 8 TeCDD 50ng mL 12 13 JIS K0557 A4 A3 14 15 104 2 3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 75cm2 1mL 5mL 1 15mL 50mL 96 ew 0mL a 48
418. 3 4 227 1 5 6 7
419. 4 2 5 ne ug mL sp 1 13 0 3 ns naa 9 8 7 10 72 8 2 208 189 6 98 369 3 2 sg ss 272 881 24 2 e 0 EHP7IETTIEY ma we al a 0 BESPHHP7EHRHBTEHHSOOTTPPGI 00 8 CINIIHEHHEFHGEHRERHECETJWY ND Not Detected ug mL CV DMSO 1 mL 1 mL DMSO 10 pL 40 pL 50 pL 50 pL 100 pL 1 10 pL 4 2 6 4 2 7 3 4 2 6
420. 8 42 DMSO 2 5mL 5mL min DMSO 1mL 4 3 9 4 DMSO 212 90 gt DMSO 4 3 9 DMSO 5 1
421. CV 4 1 11 6 l E CV 30 CV 20 2 1 2 3 4 5 6 7 8 9 10 11 12 DMSO DMSO DMSO DMSO DMSO STD STD STD STD STD 6 1 6 2 6 3 6 4 6 5 STD STD STD STD STD 5 1 5 2 5 3 5 4 5 5 STD STD STD STD STD 4 1 4 2 4 3 4 4 4 5 STD STD STD STD STD 3 1 3 2 3 3 3 4 3 5 STD STD STD STD STD 2 1 2 2 2 3 2 4 2 5 STD STD STD STD STD 1 1 1 2 1 3 1 4 1 5 4 1 10 96 4 1 6 Std ng TEQ mL 6 CV ng TEQ mL 1 2 3 4 5 Std 6 0 012 0 018 0 0071 0 011 0 014 0 016 0 012 0 00834 28 3 Std 5 0 087 0 087 0 034 0 035 0 087 0 036 0 0386 0 0015 4 2 Std 4 0 12 0 12 0 12 0 12 0 12 0 13 0 12 0 0034 2 8 Std 3 0 37 0 35 0 34 0 37 0 35 0 34 0 35 0 014 4 0 Std 2 1
422. DMSO 2 KD DMSO 60uL 180uL 3 5 1 Ah 2 3 7 8 TeCDD 2 2 1 101L
423. DMSO 0 5mL 3 DMSO 10 4 DMSO 5 DMSO DMSO lmL 6 DMSO 5 1
424. 1 1 3 n 10 3 SD CV 5 4 4 2 5 1 98 5 4 2 11 CV 4 50 26 CV 3 pg mL 1 50 3 ug mL ug mL 1000 10000 6 7 ug mL 4 2 11 199 30 20 2
425. 3 ARNT Arylhydrocarbon Receptor Nuclear Translocator Ah 4 DRE Dioxin Responsive ERlement 5 AB1 Ah 6 AB2 AB1 7 DEQ Dioxion Equivalent Quantity 2 3 7 8 TeCDD 137 3 1 E
426. 96 CYP1A1 6 Subculture 7 Confluent 8 Plasmid DNA 9 10 CV O 3 1 1 2 1 1 30 3
427. Total 1 ng mN ng TEQ mfN pg L pg TEQ L ng g ng TEQ g ND 2 1 4 0311 K0312 2 1 2 3
428. x 67 1 E cells mL 8 1 12 mL 1 24 mL 9 125 000 cells mL 1 100 G418 G418 0 4 mgmL 100 units 0 1 mg mL 2 10 G418 1 12 mL 1 200 uL 12 2 11 3 4
429. 1 3 3 2 3 3 2 2 1 JIS K0557 A4 A3 2 JIS K8891 78 3 JIS K8040 4 JIS K8680 5 JIS K8117 6 JIS K8825 7 DMSO JIS K9702
430. 11508 11115 11615 11985 12228 432 599 RLU 599x2 3 7 8 TeCDD R2 0 966 432 599x3 3 2 4 pg mL 432 599x10 7 2 pg7mL 131 RLU 25000 20000 15000 10000 5000 0 10 20 30 40 2 3 7 8 TeCDD pg ml 8 5 14 3 1 5 RLU
431. 2 JIS K8891 3 JIS K8040 3 4 JISK8680 3 5 JIS K8117 E 6 JIS K8825 3 7 3 or 3 S S HD Cr W 1 3 92 3 8 DMSO JIS K9702 1 9 JISK8180 10 JIS K8951 11 JIS K8550 12 JIS K8987 13 1 6 14 JIS K0311 6 2 H
432. 50mL 10 mL 50 mL 10 mL 200uL 1000uL _200uL 1000uL 520uL 20 200uL 200 1000uL 12 5 50uL 50 300uL II 387 5 CO2 1000 rpm 66 20 21 22 23 24 25 26 27 28 29 30 12x75 mm 2mL BICELL 80
433. 6 1 12 2 3 1 ng mN ng TEQ mN ng g ng TEQ g 2 2 1 4 2 ND E
434. JIS K8180 10 JISK8951 11 JISK8550 12 JIS K8987 13 1 6 14 JIS K0311 6 2 HD TF ll 3 HD 1 3 eas 3 231 15 44 JIS K0311 6 2 16 20 14 100 g 11 625 gL 40 mL 17 JIS K0311 6 2
435. WM 77 GmL Cpr ng TEQ g HH 4 H 3ng TEQ g 0 1ng TEQ g 50mL 0 07mL 100pg mL 0 390 0 027 _ 100 x 0 390 x 0 07 50 1 1000 50 0 1 4
436. 0 4 0 05 72N 24mL 6 6 50mL 7 0 05 0 01 0 5 pH 1 15mL 8 2 0 2M 0 1 15mL 213 9 1 10 1 2 2 1 JIS K0557 A4 A3 2 DMSO JIS K9702 3 6 3 3 4 4 77 2 1 DMSO 2 3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 450 nm
437. 192 3 4 4 101 HCI 2mol 16 3 3 4 4 R 102 4 3 3 4 5 3 4 5 1 2
438. Y X 83 2 11 Y X 1 5 4 n 41 n 20 HRGC HRMS HRGC HRMS 1 HRGC HRMS Y Y X 2 Y X 8 2 12 Y X 1 5 2 n 32 n 41 y 0 2256
439. Y X 0 356 n 54 n 41 100 100 z 10 1 b 8 01 91 MM 001 no1 1 0001 0001 o001 001 01 1 10 100 1000 0001 00 01 1 10 100 ng m N ng g 3 4 10 8 4 11 113 5 DR EcoScreen 17 92 1 5 1 DR EcoScreen 8 5 1
440. pg g pg m3N 9 3pM well 2 10 pgm3N pg g ng m3N ng g 9 XX 21 0 On ng m3N Os 11 Cs ng m3N 11 20 Os 20 C 0 6 6 1 CV 30 20 2 6 1
441. 1 1 2 3 4 5 GPS 6 7 8 2 9 10 11 2 1
442. 15mL 4 3 4 100xg 5 4 4 5 6 cells mL 7 100 000 cells mL 4 8 5mL 3 4 9 25 10 4
443. 17 EA E H4IIE 1luc CF 17 92 1 e DR EcoScreen 17 92 1 5
444. 18 19 20 3 3 1 1 MEM 1L NaHCO 2 2g 500mL 50mL x 1L FBS 10 3 80 SCBso Bsd Bsd 10 9 0 22mm
445. 2 AxB mL 43 80 3 1 4 3 1 3 8 G E 19 mL 8 FE n 1 A 1mL E 1 B 3 4 3 1 4 1 2 2 150 19 1 4 4 1 8 8 1 2 2 75 9 1 4 4 1 8 8
446. 3 DRE ARNT ARNT DRE DRE ARNT 4 DRE ARNT 5 2 4 3 4 1 12 200 pL DMSO DMSO DMSO 2 nL 1 1 2 nL x2 4 pL 5 nL 500 pL
447. 4 3 1 4 4 11 1 4 4 11 2 1 4 2 3 2 4 2 1 0 4mL 0 75mL min 3 0 75mL 0 75mL min 4
448. 2 100uL well 96 4 2 DMSO 10 E 2x106 1x107 cells mL 28 Nh 1000rpm 2 1 80C 87
449. 215 A 4 3 11 96we11 2 3 4 6 4 7 4 8 100uL well 18 25 60 4 9 6 1 5 300uL well 3
450. 3 50mL 2 4 20 5 50g 100mL 300mL 3 8 1 az
451. 50 10 k DMSO 10 100pg uL 2 3 7 8 TeCDD DMSO 2 8 7 8 TeCDD DMSO Bsd 87 Bsd 96 990uL well DMSO 10uL well 96 1 4 1 1 24 96 100uL well DMSO 1uL well DMSO 0 5 96 8 3 7 4
452. 5mL 3 100xg 3 10mL 3 4 5 5 5mL 3 2 3 6 3 3 2 1 EDTA 2mL 4 5 2 10mL
453. DMSO 0 DMSO 0 02 0 05 0 1 0 2 0 4 0 6 0 8 1 0 2 0 3 0 4 0 5 0 10 20 30 50 100pg uL DMSO 2 4 1 5 1 17 5 RLU pg TEQ uL CV 30 20 2 6 8 3 11 CV 30 30 Fs 40 CV 20 20 CV 0 01 0 1 1 10 100 pg TEQ p D 8 8 11 6 2
454. 0 PCBs PCDDs PCDFs 4 5 1 3 2 3 5 10 2 3 1 E 4mL 5 C 38 H PCDDs PCDFs PCBs 4mL 5
455. 2 1 ulL DMSO ul 1 0 8 100 200 9 8 100 200 27 9 100 200 81 27 100 200 4 5 DMSO 20 25C 20 DMSO 1 20 DMSO 50uL 50 pL 1 6 B 1 Bo B Bo y x Logit Log 1 2
456. 263 ng TCAP 26 3 ug TCAP mL 4 2 7 0 503 28 0 ug TCAP 50 3 2 800ug TCAP mL 6 2 1 1 3 1 1 0 m3N 10 mL 10 mL 0 5 mL 0 066 ng TEQ m3N 2 4 2 8 0 13 7 0 ng TEQ m3N DMSO 5 3 81 81 570ng TEQ m3N k 4 2 8
457. 3 7 8 TeCDD 50ug mL DMSO 10 CO 99 5 11 DMSO JIS K9702 2 12 EDTA 0 25 1mM EDTA 13 0 4 14 34mL DMSO 6mL 0 2um 2 DMSO 2 3 1 96 2 25cm 3 10mL 5mL 4 50mL 15mL 5 200uL 1000uL 6 200uL 7 20uL 200ul 1000uL 8 12 8 9 10 IIA
458. 4 1 2 STD1 STD7 TCP DMSO 4 1 2 STD1 STD2 STD3 STD4 STD5 STD6 STD7 ng well 5000 1250 313 78 1 19 5 4 88 1 22 0 ng mL 200000 50000 12500 3125 781 195 48 8 0 3 ll TCP 450nm TCP 172 AD 4 1 8 PC x ng mL 4 0 B C 1Cso 50 ng mL 0 1 987 100 STD7 48 8 1 825 95 3 STD6 195 1 694 87 4 STD5 781 1 212 64 3
459. STD4 3125 0 613 30 6 STD3 12500 0 164 8 9 STD2 50000 0 045 2 1 STD1 200000 0 017 0 8 TCP 4 80 20 100 1000 10000 TCP ng m 100000 1000000 4 1 8 5 2 1 ICso B Bo 50 4 1 8 STD5 B Bu 60 59 63 2 256 3 mg TEQ mL 5 4 5 4 2 5 4 4 ICs 5
460. g 12 m 0 3 0 02 g 0 5 0 02 g 3 2 6 3 1 20 mL 2 10 mL 3 4 70 mL 5 8 10 6 60 mL 1 8 50 mL 7 70 mL 8 0 5 mL
461. ul pg ul pg ul pg ul 0 0 2 0 4 0 B DMSO pg ul pg ul pg z pg ul 0 0 2 0 4 0 C SO pg ul pg ul pg z 0 0 2 0 4 0 D SO pg ul pg ul pg z 5 0 pg 7 pg ul 0 pg 0 pg 4 4 4 1 1 1 1 ul ul 7 5 E 5 5 F 5 5 pg Dg 3 G pg ul pg pg 7 z 7 pg 7 ul 3 4 5 0 5 H pg 7 pg ul pg ul pg ul pg ul pg ul BL a c b c d A n1 nl n1 n1 n1 n1 x1000 x1000 x100 x100 x100 x100 a c b c d B n2 n2 n2 n2 n2 n2 000 x1000 x100 x100 x100 x100 a c b c d C n3 n3 n3 n3 n3 n3 x1000 x1000 x100 x100 x100 x100 a c b c d D n4 n4 n4 n4 n4 n4 x1000 x1000 x100 x100 x100 x100 BL a c a b c d E n1 n1 n1 n1 n1 x10 x10 x1 X X a c d b c d F n2 n2 n2 n2 n2 x10 x10 x1 X a c b c d G n3 n3 n3 n3 n3 n3 x10 x10 x1 x1 x X a c a b c d H n4 n4 n4 n4 n4 n4 n4 x10 x10
462. wel pgm 25000 B C Mi190 1000 LNB 100 RN 20000 23 75 125 9 43 22880 11 9 62 5 8 74 19448 15000 5 94 31 3 8 05 16471 2 97 15 6 7 35 12357 G 10000 1 48 7 81 6 66 7517 0 742 3 91 5 97 4754 5000 0 371 1 95 5 27 2114 0 186 0 977 4 58 1233 0 0928 0 488 3 89 434 Va 28631 Ki 7 75 b 192 5 2 ut2o u g nu2o 1 c STD5 TeCDD 0 78ppt QC
463. 0 322 206 3 0113 7 9 0122 8 4 3 5 0 60 o iC V 0 56 0 64 E lb G 8 15 4 3 13 5 3 B Bo 20 80 ng7m3N ng g Vg vV 7 VV Cc X Xx 219 Cs ng m3N ng g X Qg mL Vz GmL Vg GnD mL m3N g
464. 0 5 2 2 3 3 1 Q150cm2 11 3 2 8 2 150cm22 19mL 8 3 50mL 1 4 10 5 3 8 6 5 COs 37 11 12 1 1 2nm 1 3 24
465. 000 92 65 Ss m3N zz pg mL DMSO Vv mL Ve mL Vz mL Czz ng TEQ m3N 4 5ng TEQ m3N 0 17ng TEQ m3N 10mL 5mL 1mL 10pg mL 0 2057 10x0 2057x1 10 1 0 024 2
466. 0523 TEQ DEQ Ve DMSO 40nL Ve 1 DMSO 2puL 23 50 mL Vg 25 mL _ 1x0 0523_ 1 30 50 1000 0033 2 23 0 063 g 139 4 1 3 6 2 2 1 JISK0557 A4 A3 2 JIS K8891 3 JISKS8040 4 JISK8680 5 JIS K8117 6 JIS K8825
467. 057x 12 R 0 8864 0 10 20 30 40 50 60 70 ng m N 3 5 15 E Y Y X F 3 5 15 Y X n 20 n 20 HRGC HRMS HRGC HRMS 1 X HRGC HRMS E Y Y X 2 Y X 3 5 16 Y X 0 2759
468. 0mL 10mL 1mL DMSO 0 018ng7mL 0 422 0 018 x0 422x1x x 0 09 4 3m3N 2 2 Q 1 80 3 1 zz ng mL DMSO Qp 204
469. 1 8 6 10mL 8 7 500rpm 10 8 PBSO 8 9 10 3 1 3 PBS 10mL cm 2 PBSC mL mL mL PBS mL 150 5 2 10 10 75 2 1 5 5 25 1
470. 1 2 3 4 5 6 7 6 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 5C 50C 5 CO 3000 4000 min A JIS T7322 JTS T7324 121C 196kPa 160 200C 10mL 10mL 50mL 25cm2 75cm2 150cm2 20uL 200uL 1000uL 10mL 96
471. 2 X HRGC HRMS Y 0 0494 3 3 6 17 41 X HRGC HRMS Y 0 0523 n 41 n 42 1000 1000 y 0 0447x 2 0 0406x R 0 9683 R 0 9941 100 0 0494 0 0523 8 a 10 no oh bn 1 3 Ma 1 0 1 0 1 0 01 0 01 0 1 1 10 100 1000 10000 0 1 1 10 100 1000 10000 ngDEQ m3N ngDEQ g 8 6 16 3 6 17
472. 2 1 1 1 1 1 1 1 2 1 1 1 1 0 064 5 7 Std 1 93 7 8 8 15 15 11 6 5 11 3 8 393 8 50 45 40 a CV 30 30 lt e gt CV 20 O 5 15 10 5 e 0 1 1 0 01 0 1 1 10 ng TEQ m7 176 4 1 11 1 160uL 25uL 6 2 1mL 4 1 8 ng TEQ mL DMSO 80uL 100uL 1 HH lt lt gt 4 1 7 DMSO mL 1 80uLL 1
473. 2 11 4 2 3 1 2 1 2 B Bo 63 9 46 6 M ug mL 183 8 357 5 SD ug m 7 85 16 43 CV 4 01 4 60 M 2SD 168 6 198 0 324 6 390 4 250 200 M 2SD M M 2SD 150 100 4 1 5 3 B Bo 50 DMSO 1 C 2 V s VV Cs ug TCAP m3N ug TCAP mL vV mL Vw mL Vg mL m3N 197
474. 20 Gu o C 1 5 1 0 0095ug mL 25C ng TCPHA mL 34 4 4 6 4 4 7 4 4 18 246 4 4 6
475. 50mm 3 6 100 3 7 0 1 10 mL 2 IE3 4 4 1 4 2 1 JIS K0311 6 4 4 4 3 JIST 232 20 mmol g 3 2 J 4 4 3 _
476. 6 2 2 5 2 885 0 2 70 nmo1l L s 5 2 1 4 4 2 10 2 ug mL 3 M SD 28D CV 20 196 4 2 3 1 2 10 4
477. 74 60000 50000 40000 30000 20000 10000 0 20 40 60 80 100 120 TCDD pg ml 8 5 12 2 3 7 8 TeCDD STD 5 2 2o pu o 1 129
478. 8 8 30 300uL 9 12 30 300uL 8 10 11 12 13 1 5mL 14 15 1 5mL 16 15mL 17 18 19 3 1 2 8C TCP 15 25 169
479. 8 5 8 8 5 8 0 01 pg well 10 pg7mL 0 02 pg well 20 pg mL 6 2 H 8 5 4 1well pg well m3N ng m3N LuL well mL 0 01 1 4 12 1 1 0 005 132
480. 99 2 97 15 6 7 35 9696 15 5 1 00 6 0 99 1 48 7 81 6 66 5195 7 82 0 338 4 33 1 00 0 742 3 91 5 97 2597 4 17 0 267 6 39 1 07 0 371 1 95 5 27 1328 2 30 0 303 7 1 18 0 186 0 977 4 58 637 0 97 0 260 26 8 0 99 0 0928 0 488 3 89 364 ND 0 0464 0 244 3 20 271 ND ls TeCDD n 5 70 60 50 40 a gt J 30 20 10 0 6 2 1 NN MM Wp 1000 V VV et 1000 V s V Cnr Cez Qpr Qar V Ve 4 0 5 0 6 0 7 0 8 0 9 0 10 0 11 0 TeCDD C LN B 100 E 3 1 14 6 1
481. C 5 6 20 1 1 4 6 1 2 1 2 DMSO DMSO 18 5C 20 25 18 mm 100 mm 800 pL 3 4 4 2 8 1 2 1
482. NS 252 1 2 000 1 H 1
483. RMS 1 1 2 3 4 1 1 JIS KO0095 JIS K0811 5 CF 11 67 2 1 1 1 1 2 1
484. V m3N g 19 1 n 2 DMSO 1 2 1 2 RLU n 2 On ng m3N Os 20 Cs Qg m3N 20 20 0s 20 8 1 7 tk RLU naNngg gmL pg 400uL m3N g 6570 6 41 6 08 2 43 100 20 6 0 405 5890 6 27 5 30 2 12 100 20 6 0 353 51 6 6 1
485. X 100 4 1 2 4mL 2 DMSO 5 5 2 4 5 TCAP 1 180 ng mL 2 360 ng mL 5 100 mL 4 1 100 mL 300 0 03 g 6 100 mL 4 1 100 mL 0 88 g 0 05 g 7 100 mL 2 1 4 26 mg 100 mL 2 2 2 100 mL 2 2 1 4 5 g 1 JIS K0557
486. g zz ng TCAP mL DMSO 12 mL Vs mL Vs mL Cpz ng TEQ g 4 3 ng TEQ g 0 1 ng TEQ g 10 mL 10 mL 0 5 mL 26 3X103 ng TCAP mL 175 5X103 1 Y HRGC HRMS Y X 2
487. induction by propanil diuron and hnuron Chemosphere 74 155 165 2008 2 2 1 coMEM Fetal Bovine Ser L 2 3 4 L um FBS 56 30 20 200mM 0 10000units 10mgmL 0 124 5 HygromycinB 50mg mL 6 1 500mL 2 25mL 3 10mL 42 5mL 5 1 5mL 7 1 500mL 2 25mL 3 10mL 4 5mL 8 9 2
488. n DMSO 1mL 4 4 9 4 DMSO 237 gt DMSO 4 4 9 DMSO 5 1 2 4 5 TCPHA
489. ut 2c kp o 1 155 3 5 1 8 pg uL pg uL 3 6 15 pg pL Co 3 6 15 5 3 1 Y
490. x0 9705 202 10 10 G E E 1 E 1 E EE fF 0 1 01 ul F 0 01 0 01 0 001 PITTTTTI i nn 0 1 1 10 100 1000 0 01 0 1 1 10 100 1000 10000 ng m3N ng g 3 2 11 3 2 12 75 3 HeB5 17 92 1 3 1 HeB5 8 8 1 3 3 1 2 1 Ah
491. x10 x1 x1 x1 x x pg ul 3 3 7 96 5 5 1 5 2 3 7 8 TeCDD 14 RLU 4 g 2 3 7 8 TeCDD RLU DMSO 0 RLU 89 RLU 0 s RLU 2 2 7 6 I 1 T cv B Y 6 8 3 8 3 8 7 y I X
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