InertSustain」へ進化
Contents
1. InertSustain C18 ODS A ODS B 2 2 Conditions Column 1 Eluent 4 Flow Rate Col TemDp Detection Sample 3 5 0 2 4 6 8 10 12 14 0 2 4 6 8 10 12 14 0 10 20 30 Time min Time min Time min ze InertSustain C18 ODS B ODS C 2 2 2 Conditions _ Column Eluent 3 Flow Rate 1 Col Temp Detection Sample 0 Time min Time min Time min InertSustain C18 ODS B 1 1 1 1 Conditions _ Column Eluent Es CE CE CE 0 2 4 6 8 10 12 14 0 Flow Rate Time min Time min Col Temp 1 Detection Sample 2 2 1 NSK 0 2 4 6 8 10 12 14 0 2 4 6 8 10 12 14 Time min Time min 3 um 150 x 2 1 mm D A CH3CN B 25 mM Kz2HPO4 pH 7 0 KH2PO4 AIB 30 70 v v 0 2 mL min 40 C UV 230 nm 1 Uracil 2 Pyridine 3 Phenol 4 Berberine chloride 5 Dextromethorphan 3 um 150 x 2 1 mm 1 D A CHsCN B 0 1 HsPO4 A B 25 75 v v 0 22. C8 3 um 5 um 350 m9g 8 100A 10nm USP EZ 0 85 mL g pH 1 10 InertSustain C18 InertSustain C8 2 Conditions _ 2 3 Column Size 5 um 150 x 4 6 mm ID Eluent A CHsOH B HzO 3 15 43 min 4 9 39 mn AIB 80 20 v v Flow Rate 1 0 mL min Col Temp 40 C Detection UV 254 nm Sample 1 Uracil 2 Toluene 3 Ethylbenzene 4 PropylIbenzene 5 7 ButyIbenzene 6 7 AmylIbenzene Time min Time min 11 1 1 pH 6 0 pH 9 5 M cera ans UE NE EE ES CE SE EE EE EE 10 20 0 10 20 Time min Time min Conditions Column InertSustain C8 5 um 250 x 4 6 mm I D Eluent A CH3CN B CH30OH C HsPO4 in HzO pH 6 0 Ammonia solution in H20 A B C 150 150 780 v v v or A CHsCN B CH OH C 0 1 Ammonia solution in HzO pH 9 5 HaPO A B C 225 225 550 v v Flow Rate 1 2 m
3. WAVE Se 1st Stage ES i 7 666 C a se ee Es me Ea nN sy Ri A a mm ua eam am ur ua Ro j A CT A a 4 mn a be gg ma PA CI Ld ws HPLC HPLC UHPLC InertSustain InertSustain C18 InertSustain C8 Sm InertSustain Pheny I InertSustain NH2
4. InertSustain Phenyl B C 2 3 1 0 10 20 30 40 0 10 20 30 40 0 10 20 30 40 Time min Time min Time min Conditions OH OH Column Size 5um 150 x 4 6 mm 1 D OH Eluent A CH OH H B H20 A A B 20 80 v vV Col Temp 40 CH CH Flow Rate 0 8 mL min 9 Detection UV 254 nm 1 o Cresol 2 m Cresol 3 p Cresol InertSustain NH2 ES E InertSustain NH2 _ 3um 5um 350m9g 0 100A 10nm USP
5. E 2 InertSustain InertSusatain C18 2 2 SUS 350 10 14 InertSustain C8 3 um 5 hm 350 10 8 7 InertSustain Phenyl jm 3 um 5 hm 350 10 10 L11 InertSustain NH2 2 3 um 5 hm 350 10 7 L8 ODS InertSustain C18 _ 2um 3um 5 um
6. 14 9 00e5 8 00e5 7 00e5 1 2 3 6 00e5 9 4 13 5 00e5 8 5 4 00e5 11 15 6 12 3 00e5 10 2 00e5 1 00e5 0 00 IrrrmmWmmmlmmmmeeemetmalrmopacanemraredemrmratprermmnumererri 0 10 20 30 40 50 Time min Time min Conditions Conditions SysStem LC800 HPLC system SysStem LC800 HPLC system 4000 Q TRAP Column InertSustain C18 5 um 250 x 4 6 mm 1 D Column InertSustain C18 3 um 150 x 2 1 mm 1 D Eluent A HzO HCOOH 90 10 Eluent A 0 1 HCOOH in CHaCN B HzO CHaCN CH OH HCOOH 40 22 5 22 5 10 B 0 1 HCOOH in HzO A B 9377 35 min 75 7 25 10 min 35 7 65 1 min 07 100 A B 2 98 0 5 min 40 7 60 5 5 min 40 7 60 v v 4 min 07100 v v Flow Rate 0 4 mL min Flow Rate 1 0 mL min Col Temp 40C Col Temp 30 Detection LC MS MS ESI Positive MRM Detection VIS 535 nm Injection Vol 2 pL Injection Vol 10 uL Sample 1 Sulpilide 9 Nortriptyline Sample Bilberry powder 1 25 mg mL 2 Milnacipran 10 Maprotiline 1 Delphinidin 3 O galactoside 9 Peonidin 3 O galactoside 3 Trazodone 11 Amitriptyline 2 Delphinidin 3 O glucoside 10 Petunidin 3 O arabinoside 4 Amoxapine 12 Trimmipramine 3 Cyanidin 3 O galactoside 11 Peonidin 3 O glucoside 5 Doxepin 13 Fluoxetine 4 Delphinidin 3 O arabinoside 12 Malvidin 3 O galactoside 6 Desipramine 14 Sertraline 5 Cyanidin 3 O glucoside 13 Peonidin 3 O arabinoside 7 Fluvoxamin
7. 5 um Cat No 6 0 Xx 50 5020 16075 33 000 5020 16076 44 000 10 x 50 5020 16077 66 000 14 Xx 50 5020 16078 83 000 5020 16079 100 000 5020 16070 190 000 5020 16073 220 000 5020 16074 300 000 InertSustain C8 EX mm 5020 16184 5020 16186 5020 16188 55 000 3 Hm 5020 16185 5020 16187 5020 16189 70 000 50 5020 16082 5020 16084 5020 16086 55 000 5 hm 150 5020 16083 5020 16085 5020 16087 70 000 EX Cat No 5020 01880 7 500 EX Nano 0 05 mm 0 075 mm 0 1 mm 02mm mm 58 000 i 73 000 78 000 58 000 5 hm 73 000 250 5020 16092 5020 16095 5020 16098 5020 16101 76 000 19 InertSustain PhenyI 55 000 55 000 pa 3 nm 60000 E50MPa 1o 60000 3 mm 150 65 000 250 5020 16522 5020 16528 5020 16534 75 000 1 16 LC NR_ UPLC UP
8. Time min Conditions Column Size 5 pm 150 x 4 6 mm 1I D Eluent A CHsCN B 0 1 HaPO4 A B 25 75 v v Flow Rate 1 0 mL min Col Temp 40 Detection UV 254 nm Sample 1 Brilliant Blue FCF 2 Phenol 3 Salicylic acid 2 um 10 0 MPa 4 6 Time min Time min Conditions Column Size 50 x 2 1 mm D Sample 1 Uracil Eluent A CHsOH 2 Pyridine B 25 mM phosphate buffer pH 7 0 3 Phenol A B 30 70 v v 4 Berberine chloride Flow Rate 0 2 mL min 5 Dextromethorphan Col Temp 40C Detection UV 230 nm 0 2 4 6 10 12 Time min Conditions Column Size 5um 150 x 4 6 mm D Eluent A CHsCN B 0 1 HsPO A B 40 60 v v Flow Rate 1 0 mL min Col Temp 40C Detection UV 254 nm Sample 1 Hinokitiol a ai 3 InertSustain C8 InertSustain C18 ES
9. E 1 9 4 Conditions Column InertSustain NH2 Sample 5 um 250 x 4 6 mm I D 1 2 Tocopherol Eluent A n Hexane B Ethyl acetate 2 Tocopherol A B 70 30 v v 3 y Tocopherol Flow Rate 1 0 mL min 4 2 Tocopherol Col Temp 30 each 25 mg L Detection UV 290 nm 0 2 4 6 8 10 Injection Vol 10 uL Time min 13 3 pH 1 2 Conditions Column InertSustain C18 5 um 150 x 4 6 mm 1I D Eluent A CH3CN B 20 mM Phosphate Borate Acetate Buffer AIB 30 70 v v Flow Rate 1 0 mL min Col Temp 40C Detection UV 254 nm Sample 1 2 6 Dimethylpyridine 2 Aniline 3 Benzoic acid 4 Acetophenone 5 3 5 Dimethylbenzoic acid 0 10 20 Time min 0 UP 1 7 0e5 7 0e5 6 0e5 FM 6 0e5 5 0e5 F 5 0e5 oS oS 4 0e5 0 gt 4 0e5 cS J 3 0e5 3 0e5 1 2 0e5 2 0e5 1 0e5 2 1 0e5 14 1 0 1 0 1 HCOOH in HzO pH 2 5 2 0 3 0 4 0 1 0 Time min 2 0 3 0 4 0 Time min 2 0 1 Ammonia solution in HzO pH 9 5 Condit
10. mm 60 75 2 mm CatNo CatNo 50 000 5020 07362 60 000 5020 14252 85 000 5020 14262 100 000 5020 14272 130 000 SHm 100 5020 07354 62 000 5020 07364 65 000 5020 14254 110 000 5020 14264 160 000 5020 14274 230 000 1 0 0 50 5020 07355 62 000 5020 07365 75 000 5020 14255 132 000 5020 14265 190 000 5020 14275 260 000 InertSustain C18 Ne 5020 07357 33 000 5 um 7 6 X 50 5020 07367 44 000 10 x 50 5020 14257 66 000 14 x 50 5020 14267 63 000 5020 14277 100 000 InertSustain C18 EX mm 5020 11539 5020 11639 5020 11739 55 000 3 Hm 5020 11589 5020 11689 5020 11789 70 000 50 5020 11538 5020 11638 5020 11738 55 000 5 hm 150 5020 11588 5020 11688 5020 11788 70 000 EX Cat No 5020 01880 7 500 gt Hl EX Nano 0 05 0 075 0 1 0 2 ID 0 05mm mm f mm f mm 58 000 nm 73 000 78 000 58 000 5 hm 73 000 2 50 5020 15137 5020 15287 5020 15437 5020 15587 78 000 EX Nano Cat No 5020 01881 8 000 17 InertSustain C8
11. 350m2g 14 100A 10nm USP 3 0 85 mL g pH 1 10 pH pHT 10 InertSustain C18 ODS InertSustain C18 FI OW gt CHsCN 100 10 InertSustain C18 InertSustain C18
12. 4 6 mm EE 2 mm mm 1 0 RS UP 30 5020 16468 5020 16474 51 000 51 000 60000 60000 159 65 000 75 000 3 um mm 2 1 3 0 4 0 4 6 30 5020 16432 5020 16439 44 000 44000 50 000 50000 150 55 000 65 000 mm 1 0 1 5 46 00 46000 55 000 55 000 159 58 000 66 000 5 um 40000 40000 45 000 45 000 150 5020 16306 5020 16313 5020 16320 5020 16327 50 000 250 5020 16307 5020 16314 60 000 1 2 1 16 InertSustain Phenyl mm mm 1 0 15 2 1 2 1 3 0 4 0 4 6 2 1 3 0 4 0 4 6 30 MPa 1h 20 E E2 1 9 a 34 000 34 000 3 0 5020 16505 5020 16404 14 000 5020 1
13. 50 MPa 00 5020 16220 5020 16226 5020 16232 60 000 3 mm 150 5020 16221 5020 16227 5020 16233 65 000 250 5020 16222 5020 16228 5020 16234 75 000 1 16 LC eS UPLC UP 4 6 mm Ej mh mm 2 1 3 0 5020 16217 5020 16223 5020 16229 55 000 ON 9 HP 5020 16218 5020 16224 5020 16230 55 000 3 um 5020 16219 5020 16225 5020 16231 60 000 2 mm wm 10 15 UP 51 000 51 000 60 000 100 5020 16171 5020 16177 60 000 150 5020 16172 5020 16178 65 000 250 75 000 3 hm 44 000 44 000 50 000 100 5020 16135 5020 16142 5020 16149 5020 16156 50 000 125 5020 16855 5020 16856 53 000 55 000 250 65 000 30 5020 16039 5020 16045 46 000 50 5020 16040 5020 16046 46 000 75 5020 16041 5020 16047 55 000 100 5020 16042 5020 16048 55 000 58 000 250 66 000 5 hm 30 5020 16002 5020 16009 5020 16016 5020 16023 40 000 50 5020 16003 5020 16010 40 000 45 000 45 000 125
14. InertSustain C18 14 13 12 11 10 MPa 5 KN gt F G ODS ODS ODS ODS ODS ODS ODS J K L Conditions Column Size 5 pm 250 x 4 6 mm 1I D Eluent Flow Rate Col TemDp A CH OH B HzO A B 807 20 v v 1 0 mL min 40 C InertSustain C18 Lot 1 Lot 3 0 2 4 6 8 10 Time min Conditions Column Size 5 pm 250 x 4 6 mm 1I D Eluent A CHsCN B 25 mM phosphate buffer pH 7 0 AIB 40 60 v v Flow Rate 1 0 mL min Col Temp 40C Detection UV 220 nm Sample 1 Dextromethorphan
15. L8 0 85 mL g pH 2 7 5 InertSustain NH2 DN 105 InertSustain NH2 NH2 A NH2 B 4 95 90 85 9 80 0 1000 2000 3000 4000 5000 6000 0 10 20 mL Time min Conditions Conditions Column Size 5um 250 x 4 6 mm 1 D Sample 1 Maltose Column Size 5 pm 250 x 4 6 mm 1I D Sample 1 Fructose Eluent A CHsCN Eluent A CH3CN 2 Glucose B H20 B Hz2O 3 Sucrose A B 75 25 v v A B 65 15 v v 4 Moltose Flow Rate 1 0 mL min Flow Rate 1 0 mL min Col Temp 40C Col Temp 40C Detection RI Detection RI 1 2 Conditions 3 Column InertSustain NH2 Sample 1 Maltose 5 um 250 x 4 6 mm 1 D 2 Maltotriose 4 5 Eluent A CH3CN 3 Maltotetraose 6 B H20 4 Maltopentaose AIB 65 35 v v 5
16. mm 1 0 1 0 5020 16807 5020 16706 18 000 5020 16808 5020 16707 34 000 5 2 8 1 5 5020 16809 5020 16708 18 000 5020 16810 5020 16709 34 000 10 2 1 3 0 3 0 5020 16805 5020 16704 14 000 5020 16806 5020 16705 30 000 4 0 4 6 4 0 5020 16803 5020 16702 14 000 5020 16804 5020 16703 30 000 2 1 3 0 5020 16813 5020 16712 19 800 5020 16814 5020 16713 39 000 3 0 4 0 5020 16811 5020 16710 19 600 5020 16812 5020 167 11 39 000 4 0 4 6 30 MPa 22 InertSustain NH2 5 hm mm 6 0 7 6 20 50 000 5020 16659 60 000 5020 16663 85 000 5020 16667 100 000 5020 16671 62 000 5020 16660 65 000 5020 16664 110 000 5020 16668 160 000 5020 16672 1 132 000 5020 16669 130 000 230 000 260 000 0 0 50 5020 16657 62 000 5020 16661 75 000 5020 16665 154 000 InertSustain NH2 5 um Cat No 6 0 x 50 5020 16675 33 000 5020 16676 44 000 10 x 50 5020 16677 66 000 14 Xx 50 5020 16678 63 000 5020 16679 100 000 5020 16670 190 000 5020 16673 220 000 5020 16674 300 000 InertSustain NH2 EX mm 5020 16784 5020 16786 5020 16788 55 000 3 Hm 5020 1
17. 0 oO 1 3 um C C 0 5020 16732 5020 16739 5020 16746 5020 16753 44 000 0 5020 16733 5020 16740 5020 16747 5020 16754 44 000 5 5020 16734 5020 16741 5020 16748 5020 16755 50 000 100 5020 16735 5020 16742 5020 16749 5020 16756 50 000 150 5020 16736 5020 16743 5020 16750 5020 16757 55 000 250 5020 16737 5020 16744 5020 16751 5020 16758 65 000 46 000 46 000 55 000 100 5020 16642 5020 16648 55 000 150 5020 16643 5020 16649 58 000 66 000 5 um mm 40 000 50 5020 16603 5020 16610 5020 16617 5020 16624 40 000 75 5020 16604 5020 16611 45 000 100 5020 16605 5020 16612 5020 16619 5020 16626 45 000 150 5020 16606 5020 16613 5020 16620 5020 16627 50 000 250 5020 16607 5020 16614 5020 16621 5020 16628 60 000 1 2 1 16 InertSustain NH2 mm mm E E2 1 E
18. HPLC LC MS InertSustain ne 2012 6 hl Ciences HPLC LC MS mm INP Nn TMTILCLLELEL Inertsil InertSustain Contents iDSUStain wm tt 2 InertSustain C1 8 eo 4 gt 4 InertSustain C8 EE 8 InertSustain Phenyl 95oo oooooo CE O InertSustain NH2 5207CeYSKcfe22S55M2 se 10 Eees ee 1 1 2 11 13 8 282SCG ee 14 15 16 929 In er Sustain 2 LC InertSustain Inert Sustain ODS SD DE 5 a a llStIStad ES 7
19. 0 Column InertSustain C8 3 um 150 x 2 1 mm ID Eluent 0 1 HCOOH 5 mM HCOONH4 in CH OH 2 5 Flow Rate 0 2 mL min Col Temp 40C 2 0 Detection LC MS MS ESI Negative MRM 1 5 lt Injectlon Vol 2 puL 4 Sample 1 Phosphatidyl L Seryne PS 10 hg mL 1 0 2 L o PhosphatidyI DL glycerol Distearoy PG 0 2 ug mL 3 L Phosphatidylcholine PC 0 05 ug mL 0 5 4 L Phosphatidylethanolamine Distearoyl PE 0 5 ug mL 0 0 3 0 0 1 0 2 0 3 0 4 0 5 0 6 0 7 0 Time min InertSustain Pheny 1 2 4 1 2 3 5 2 Prednisolone T T T T T T T T T T T T T T T T T T T T T T 1 T T T T T T T T T T T T T T T I T T T T T I T T T T T T T T T T T T 0 10 20 30 0 10 20 30 40 Time min Time min Conditions Conditions Column InertSustain Phenyl Sample Column InertSustain Pheny 5 um 150 x 4 6 mm I D 5 um 250 x 4 6 mm I D 1 Acesulfame potassium Eluent A CH OH Eluent A CH3CN 2 Saccharin B HzO B 0 2 HCOOH in HzO 3 Sorbic acid A B 407 60 v v A B 15 85 v vV 4 Benzoic acid Flow Rate 0 8 mL min Flow Rate 1 0 mL mn 5 Dehydroacetic acid Col Temp 40 C Col Temp 25 C each 10 mg L Detection UV 220 nm Detection UV 230 nm Injection Vol 5 puL Injection Vol 10 pL InertSustain NH2
20. oo gt 1 0e4 0 N 2 0 0 Time min 1 5e4 1 oO 1 0e4 gt Cc w 5 0e3 1 0 0 0 5 1 0 1 5 2 0 2 5 3 0 3 5 4 0 4 5 5 0 5 5 6 0 Time min Conditions System LC800 HPLC System Column ODS 100 x 2 1 mm I D Eluent A 2 mM CHaCOONH4 in 95 CH3CN B 2 mM CHsCOONH4 in 10 CHsCN A B 20 80 2 min 100 0 2 5 min 100 0 0 01 min 20 7 80 v v Mixed by a gradient mixer Flow Rate 0 3 mL min Col Temp 40C Detection LC MS MS 4000 Q TRAP ESI Positive MRM Injection Vol 10 pL Sample 1 Nitrofurazone 100 ug L 2 Lasalocid A 100 ug L 12 1 Conditions Column InertSustain C18 5 um 125 x 3 0 mm 1I D 2 Eluent A CHaCN B Hz2O C CHsCOOH A B C 500 500 7 1 v v v Flow Rate 0 4 mL min Col Temp 25C Detection UV 225 nm Injection Vol 10 pL Sample 1 Valsartan 50 mg L 2 Diclofenac sodium 60 mg L 0 2 6 8 10 12 14 Time min 0 5 1 0 1 5 2 0 2 5 3 0 3 5 4 0 4 5 5 0 5 5 6 0 InertSustain C8 x1 000 5 0 4 5 4 0 3 5 4 Conditions System Nexera LCMS 8030 3
21. 4 5020 07444 50 000 502007445 55 000 502007446 65 000 30 5020 14201 5020 14211 46 000 5020 14202 5020 14212 46 000 55 000 55 000 58 000 66 000 30 5020 07311 5020 07321 5020 07331 5020 07341 40 000 5020 07312 5020 07322 40 000 45 000 45 000 47 500 50 000 5020 07336 5020 07346 60 000 1 2 1 16 InertSustain C18 mm 1 0 2 2 30 4 0 4 6 2 1 3 0 4 0 4 6 Sc0 u E E2 1 mm 1 0 5020 19250 5020 19249 18 000 5020 19300 5020 19299 34 000 10 5 5020 19350 5020 19349 18 000 5020 19400 5020 19399 34 000 3 0 5020 19150 5020 19149 14 000 5020 19200 5020 19199 30 000 4 0 5020 19050 5020 19049 14 000 5020 19100 5020 19099 30 000 3 0 5020 19550 5020 19549 19 600 5020 19600 5020 19599 39 000 20 4 0 5020 19450 5020 19449 19 600 5020 19500 5020 19499 39 000 30 MPa 16 InertSustain C18
22. 48 000 50 000 250 60 000 1 2 1 16 InertSustain C8 E E E2 1 mm mm mm ps 1 0 5020 16207 5020 16106 18 000 5020 16208 5020 16107 34 000 10 1 0 1 5 241 34 000 2 1 3 0 3 0 5020 16205 5020 16104 14 000 5020 16206 5020 16105 30 000 4 0 4 6 4 0 5020 16203 5020 16204 5020 16103 30 000 39 000 39 000 2 1 3 0 4 0 4 6 30 MPa 18 InertSustain C8 5 hm mm mm CarNo 6 0 20 50 000 5020 16059 60 000 5020 16063 85 000 5020 16067 100 000 5020 16071 62 000 5020 16060 65 000 5020 16064 110 000 5020 16068 160 000 5020 16072 1 132 000 5020 16069 130 000 230 000 260 000 0 0 50 5020 16057 62 000 5020 16061 75 000 5020 16065 154 000 InertSustain C8
23. 6506 5020 16405 30 000 4 0 5020 16503 5020 16504 5020 16403 30 000 39 000 39 000 InertSustain PhenyI mm eo 76 2 FE 130 000 5 hm 230000 150 5020 16357 62 000 5020 16361 75 000 5020 16365 132 000 5020 16369 190 000 5020 16373 260 000 InertSustain Phenyl 6 0X50 _ 7 6 X 50 5 um Cat No 5020 16375 33 000 5020 16376 44 000 10 x 50 5020 16377 66 000 14 Xx 50 5020 16378 83 000 5020 16379 100 000 InertSustain Phenyl EX mm 5020 16484 5020 16486 5020 16488 55 000 3 Hm 5020 16485 5020 16487 5020 16489 70 000 5020 16382 5020 16384 5020 16386 55 000 5 hm 150 5020 16383 5020 16385 5020 16387 70 000 EX Cat No 5020 01880 7 500 EX Nano mm 58 000 5 nm 73000 78 000 58 000 5 um 73 000 78 000 EX Nano Cat No 5020 018681 6 000 21 InertSustain NH2 1 0 5020 16768 5020 16774 51 000 0 5020 16769 5020 16775 51 000 5 5020 16770 5020 16776 60 000 100 5020 16771 5020 16777 60 000 150 5020 16772 5020 16778 65 000 250 5020 16773 5020 16779 75 000 RE mm 21 mm
24. 6785 5020 16787 5020 16789 70 000 50 5020 16682 5020 16684 5020 16686 55 000 5 hm 150 5020 16683 5020 16685 5020 16687 70 000 EX Cat No 5020 01880 7 500 EX Nano 0 05 mm 0 075 mm 0 1mm 0 2 mm mm 5020 16791 5020 16794 5020 16797 5020 16800 58 000 3 um 5020 16792 5020 16795 5020 16798 5020 16801 73 000 5020 16793 5020 16796 5020 16799 5020 16802 78 000 5020 16690 5020 16693 5020 16696 5020 16699 58 000 5 um 5020 16691 5020 16694 5020 16697 5020 16700 73 000 250 5020 16692 5020 16695 5020 16698 5020 16701 78 000 EX Nano Cat No 5020 01881 6 000 23 HPLC http WwWww gls co jp hplc hntml OW TL HL ciences TEL TEL TEL TEL TEL 03 5323 6611 TEL TEL TEL TEL TEL TEL 052 TEL TEL 06 6357 5060 045 985 7900 024 534 2191 029 858 3700 048 667 1611 043 248 2441 931 1761 1101 5200 082 092 233 291 04 2934 2121 04 2934 1100 024 533 2244 FAX 03 5323 6622 FAX 06 6357 4580 FAX 045 985 7901 FA
25. 7 TE 7 A 0 EE 1st Stage Evolutionally Surfaced Silica ES ES 2nd Stage ES 3rd Stage C18 C8 ES ES
26. L min Col Temp 46 C pH 6 0 or 25 pH 9 5 Detection UV 210 nm Sample 1 Lincomycin 1 InertSustain Pheny C18 C8 Pheny 7 7 7 3um 5um 350m9g 10 100A 10nm USP 11 0 85 mL g pH 2 7 5 InertSustain C18 InertSustain Phenyl A 2 4 4 1 3 4 2 13 1 2 3 0 20 40 60 80 0 10 20 30 0 10 20 Time min Time min Time min Conditions Column Size 5 pm 150 x 4 6 mm 1I D Eluent A CHsOH B HzO AIB 70 30 v v OO Flow Rate 0 8 mL min J Col Temp 40 Detection UV254 nm 1 Butylpenzene 2 o Terphenyl 3 Amylbenzene 4 Triphenylene
27. Maltohexaose Flow Rate 1 0 mL min 6 Maltoheptaose Col Temp 30 C Detection RI WE EE Ee Ee CC CE CE EE EE 0 2 4 6 8 10 12 14 Time min pH InertSustain C18 InertSustain Inertsil 1 4 InertSustain C18 Inertsil ODS 3 Inertsil ODS 4 1 3 InertSustain C8 Inertsil C8 3 Inertsil C8 4 1 0 Terphenyl 4 InertSustain Phenyl pneny 4 Inertsil Ph 3 tf 1 A 7 3 Triphenylene 0 9 Conditions Column Size 5um 150 x 4 6 mm I D Sample Eluent A CH OH 1 o Terphenyl B Hz2O 2 n Amylbenzene 9 89 0 A B 70 30 v v 3 Triphenylene K Amylbenzene Flow Rate 0 8 mL min Col Temp 40 C Detection UV 254 nm ta UE Es LC MS MS
28. X 024 536 1518 FAX 029 858 3780 FAX 048 667 1656 FAX 043 248 2485 FAX 052 931 1814 FAX 082 233 1110 FAX 092 291 2552 FAX 04 2934 2128 FAX 04 2934 3361 FAX 024 534 2139 _ TM 163 1130 6 22 1 30F TEL 03 5323 6611 FAX 03 5323 6622 http www gls co jD E mail info gls co jp 20120608KH10T
29. apsaicin A B 5 95 5min 95 7 5 v v 3 Theophyline 8 Chlorpheniramine 13 Reserpine 18 Dihydrocapsaicin 40 C 4 Scopolamine 9 Propranolol 14 Isopropylantipyrine LC MS MS 5 Naltrexone 10 Diphenhydramine 15 Ketoprofen 4000Q TRAP ESI Positive MRM 9 InertSustain C18 pH ODS 0 5 pH 2 pH 10 35 mg 1 8 M Time min Time min conditions _ System PLC761 system Flow Rate 18 9 mL min J J Column InertSustain C18 5 um 150 x 20 mm 1I D Col Temp 35C Eluent A CH3CN Detection UV 465 nm g
30. e 15 Clomipramine 6 Petunidin 3 O galactoside 14 Malvidin 3 O glucoside 8 Imipramine each 100 ng mL 7 Cyanidin 3 O qrabinoside 15 Malvidin 3 O arabinoside 8 Petunidin 3 O glucoside 11 InertSustain C18 2 1 0 2 4 6 8 10 Time min Conditions _ Column InertSustain C18 5 um 150 x 4 6 mm I D Eluent A CH3CN B 2 1 CHaCOOH A B 40 60 v v Flow Rate _ 2mL min Col Temp 40C Detection UV 254 nm Injection Vol 20 uL Sample 1 Glycyrrhizic acid dipotassium salt 250 mg L 2 Propyl p hydroxybenzoate 50 mg L InertSustain C18 3 um 100 2 1 2 4 6 8 10 12 Time min Conditions _ Column InertSustain C18 Sample 5 um 250 x 4 6 mm 1I D 1 Oxalic acid Eluent 10 mM NH4HzPO pH 2 6 HsPO4 2 Tartaric acid Flow Rate 1 0 mL min 3 Glycolic acid Col Temp 40C 4 Malonic acid Detection UV 210 nm 5 Lactic acid Injection Vol 10 uL 6 Acetic acid 7 Fumalic acid 8 Succinic acid 9 Acrylic acid 14 100 mg L 1000 mg L 1000 mg L 1000 mg L 1000 mg L 1000 mg L 1000 mg L 100 mg L ODS M 2 um 1 5e4 1
31. ions System LC800 HPLC system Column InertSustain C18 3 um 75 x 2 1 mm 1 D Eluent 1 CHaCN 0 1 HCOOH in HzO pH 2 5 10 90 v v 2 CHaCN 0 1 Ammonia solution 28 in H2O pH 9 5 30 70 v v Flow Rate 0 3 mL min Col Temp 40 Detection LC MS MS Sample 4000 Q TRAP ESI Positive MRM 1 Ranitidine 2 Sulpiride 3 m HP HP 50 MPa 3 um 2 um 3 hm 2 um Intensity cps Intensity cps Conditions SysStem Column Eluent Col Temp Detection InertSustain C18 2 um 50 Xx 2 1 mm 1 D 12eg 500uL min 34 4 MPa 14 1 0e6 8 0e5 6 0e5 4 0e5 2 0e5 0 0 0 5 1 0 1 5 2 0 2 5 3 0 3 5 4 0 4 5 5 0 Time min InertSustain C18 HP 3 um 75 x 2 1 mm 1 D 750 uL min 20 3 MPa 14 1 2e6 1 0e6 8 0e5 6 0e5 4 0e5 2 0e5 0 0 0 5 1 0 1 5 2 0 2 5 3 0 3 5 4 0 4 5 5 0 Time min LC800 HPLC system InertSustain C18 or InertSustain C18 HP A CHaCN 1 Acetaminophen 6 Caffeine 11 Doxepin 16 Warfarin B 0 05 HCOOH in HzO 2 Ranitidine 7 Metoprolol 12 Amitriptyline 17 C
32. mL min 40 C UV 254 nm 1 Brilliant Blue FCF 2 Phenol 3 Salicylic acid 5 um 150 x 4 6 mm 1 D A CHsCN B 0 1 HsPO4 A B 40 60 v v 1 0 mL min 40 CO UV 254 nm 1 Hinokitiol ES HPLC pH InertSustain C18 ODS InertSustain C18 InertSustain C18 D E 100 0 50 100 150 h 200 250 300 Column Size Eluent Flow Rate Col Temp CO Eluent Flow Rate Col Temp Detection Sample 5pum 150 x 4 6 mm I D A 50 mM Triethylamine pH10 0 B CHsOH A B 70 30 v v 1 0 mL min 50 C A CH3CN B H20 AIB 65 35 v v 1 0 mL min 40 C UV 254 nm Naphthalene
33. t A B 0 5 Formic acid Sample Berberine or 25 mM Ammonium bicarbonate mi A B 5 95 15min 65 35 v v 15 InertSustain C18 mm 2 1 3 0 2 um 80 MPa HP 3 um 50 MPa 1 16 LC UHPLC UPLC 5020 14351 5020 14361 5020 14352 5020 14362 5020 14354 5020 14364 5020 14355 5020 14365 62 000 62 000 68 000 68 000 76 000 ee0 3 mm NN ed 2 mm UP 55 000 55 000 60 000 60 000 150 5020 14415 5020 14425 5020 14445 65 000 5020 14416 5020 14426 75 000 UP 4 6 mm 3 um 5 um mm 1 0 NS 51 000 51 000 60 000 100 5020 14304 5020 14314 60 000 5020 14305 5020 14315 65 000 75 000 44 000 50 5020 07412 5020 07422 5020 07432 5020 07442 44 000 75 5020 07413 5020 07423 5020 07433 5020 07443 50 000 100 5020 07414 5020 07424 5020 0743
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