pBAsi-hU6 Pur DNA
Contents
1. v201007Da 077 543 6116 PE Raabe TaKaRa Fe ss2. 6170 pBAsi pBAsi hU6 Pur DNA Cal EcoRV EcoRI promoter human U6 BamH l 2 21 EHL seer Hind Il pBAsi hU6 Pur 3 868 bp SV40 promoter 10 mM Tris HCl pH8 0 1 mM EDTA HR 3868bp OnE 1 dideoxy 2 z77H EcoRI IET 1 APA Cla EcoRV 2 0 1 Brummelkamp etal 2002 Science 296 550 553 2 Leeetal 2002 Nat Biotech 20 500 505 3 Paddison et a 2002 Genes and Dev 16 948 958 4 Paul et al 2002 Nat Biotech 20 505 508 5 Sui et al 2002 Proc Natl Acad Sci USA 99 5515 5520 6 Kawasaki et al 2003 Nucleic Acids Res 31 700 707 7 Tuschl T etal 2003 Nat Biotech 20 446 44 8 Shen C et al 2003 FEBS Lett 537 111 114
3. 20 C 2 Lot No pBAsi hU6 Pur DNA RNA polymerase Ill pol III human U6 SIRNA pol lll RNA DNA siRNA RNAi siRNA RNA Adenovirus Expression Vector Kit Dual Version Ver 2
4. 40 promoter Form 10mM Tris HCl pH8 0 1mM EDTA Preparation Purified by ion exchange column treatment Chain length 3 868 bp Purity 1 Confirmed to maintain promoter and cloning site by dideoxy sequencing method 2 Confirmed to be cleaved at a single site by restriction enzymes BamH and EcoRI and at two sites by restriction enzymes Cla and EcoRV Reference 1 Brummelkamp etal 2002 Science 296 550 553 2 Lee et al 2002 Nat Biotech 20 500 505 3 Paddison et a 2002 Genes and Dev 16 948 958 4 Paul et al 2002 Nat Biotech 20 505 508 5 Sui et al 2002 Proc Natl Acad Sci USA 99 5515 5520 6 Kawasaki er al 2003 Nucleic Acids Res 31 700 707 7 Tuschl T etal 2003 Nat Biotech 20 446 44 8 Shen C et al 2003 FEBS Lett 537 111 114 Note This product is for research use only It is not intended for use in therapeutic or diagnostic procedures for humans or animals Also do not use this product as food cosmetic or household item etc Takara products may not be resold or transferred modified for resale or transfer or used to manufacture commercial products without written approval from TAKARA BIO INC If you require licenses for other use please contact us by phone at 81 77 543 7247 or from our website at www takara bio com Produced by TAKARA BIOTECHNOLOGY DALIAN CO LTD v201007Da pBAsi hU6 PurDNA Code No 3224 Size 20 ug Shipping at 20 C Store at
5. pBAsi hU6 Pur DNA Code No 3224 Size 20 ug Shipping at 20 C Store at 20 C 2 years from date of receipt under proper storage conditions Lot No Concentration g l Volume ul Regarding protocol please refer to the product manual supplied with this vector Description pBAsi hU6 Pur DNA is a plasmid vector containing a RNA polymerase III pol III promoter human U6 for expression of siRNA and a puromycin resistant gene By inserting synthesized oligo DNA sequence for hairpin RNA into the downstream of the pol lll promoter siRNA expression vector is constructed The obtained plasmid is transfected into cells to perform RNAi experiment By selecting puromycin resistant cells siRNA stable expression cells can be established Moreover the region composed of the promoter and hairpin RNA sequence can be excised and be easily put on adenovirus vector plasmid for the construction of siRNA expression recombinant adenovirus Adenovirus Expression Vector Kit Dual Version Ver 2 Cat 6170 can be used for this purpose Several kinds of plasmid vector are available in pBAsi vector series Each carries each defferent promoter and antibiotic resistant gene A customer can choose an appropriate vector depending ona kind of target cell and an experimental purpose Restriction enzyme map cal EcoRV EcoRI promoter human U6 BamHl bal E Sesser HindIII R Pur pBAsi hU6 Pur 3 868 bp SV
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