ノロウイルスGⅠ検出試薬キット ノロウイルスGⅡ検出試薬キット
Contents
1. Jt UL L DU 3i muuautututututuu t jDNasq 00 0000 0000 ET DE ET OU O O BL EL ELE unngugnuunuunnugununnp Uu i uiu utu t VU D 00 0000 UD Zo DG Lum nm 1 IEE S mS Sm L 1 L lie He L3 L3 r3 r3 3 L3 r3 rt Ej g l rH L3 mal E usa sd O 1 L3 cH Ten 2 r3 32 21 H L3 r1 1 Lj 11 x 3 rr a L4 L1 1 1 3 L3 3 Er o00 5 E 1 LJ g 2 1 rc 2 ron 3 H lt 1 ES go E 1 oa aqa L1 L3 2 O0 pg m oH 1 m a LH um 3 uu OZO oga 11105 m Le E z EraSs aas E Lr HgEdegesblagsupmuoiuma go 52 rrj Og 1 L Dm 1 609 L1 ag E33 ma Hg L I pi Og Ld 1 SEE C Es T ps Ed E2. CDNALT ET EJ JII EF EC D B DE BB I 0 EL RNATT E O0 T O dul HELD bNATL TG IB UU ELO UO UO HELLO 000 uuum 00000000 1601 000 00000 000000001 UL s Hau s I Hl L EL L Viral RNA Mini Kit JOiagen ngngnaunguunnununmmoeuunu 55006100 1000 0000 200 100 00000 0000 00000000000 000000 H 0 63 160 D s 00 0000000000 00000 0 100000000 iuuauuuduatllutliliullii LAMPO O O 8 C 10LAMPO rry rj LT r rir LL rrr rrr rr 4 eill ulilililiililiiuliiitiiuililil liil ll III UI II lI L 00000 LAMPII 0000000 000000000 UJU L O 010 0 00 Viral RNA Mini Kit Qiagen L4 LAMPTI Loopamperi nnn gd 000000000 300 N T i 2008000000 Viral RNA Mini Kit Qiagen 2x Reaction Mix NV RM 1 Primer NVGI
3. i gt P mum oog Lp m 1 r3 L3 L3 r3 o oG AO O C3 E o EJ r3 L3 r3 C3 E3 C r3 r4 rn Lm o o r3 L3 o H 3 L3 a a o0 ma m 1 C3 r3 O mr LE L1 09 t p Ga Ern E 3 goong L3 tL L 2 1 LJ L1 1 LJ a o pz H 1 A r4 1 m p m ggg QO aa n g r3 50 O0 mn wi C r3 L1 C m H r3 L5 3 E 1 p4 1 C LL 10 LIL C 1 p e L3 L3 1521 gva m 1 1 H 1 A p Ln L1 L1 L3 C c O L 1 L1 O IL L1 H L1 L3 Ld 1 E c uu o E Er rm o E EJ 2 9 Hog L3 r3 E Li pH O og L3 p E L1 1 1 o d g a aA E oaa r4 Ed O L1 r4 m 3 i C30 r3 L3 rJ C3 r3 r3 Ooa a GO GOAK 3 r3 3 C r3 L1 Ep oO 2 1 00 1 p L3 E E 0 C aB EJ DIEI LE EM Hoga Han Opogona HW 2 E rc B9 oooopooogoa OS poen aaa oong oHm gog amp OGO Q L1 3 lt Arn
4. o 2525255255525055050525050555550550550 0 0 0 0 0 050 E oer eA 9000000009000 10 00440 0 4 0 0 0 0 0 0 0 4 0 0 4 0 0 4 0 0 0 0 010 OC o POOO DOR COGO LJ eo L 552225452155 L L 5 e 25 e 3 L3 r3 aaa rp E r3 LL E E BE B 8 ht X X X x O0 1900 000100000 Loopamp 22 000000 20061101 000 Loopa 00 00 001 0000000 00 00 000 0000000 Fj r pal gsm een e 007 lt lt D 3 0O Wi 3 m 1 1 Bst DNA Pol yner ase TTTITITI Enzyne M x Posi ti ve Control 2x Reaction Bst DNA Pol yner ase D stilled M Mx DNA Posi ti ve Control I LU D CEEEEEEETTETTTTTU L3 L3 L3 L3 L3 L3 L3 L1 A 3 rm L A uuaguuliliiili uli uttutbt tlt Ld 1 sam o mpm On ODofo LIC OCG O r3 a c oa E E rac gor 0 L3 L3 L1 o8 L3 HA L3 a 3
5. ib uium 090000 000000000 DU D DNALTRNAL 00000000 s DDDID 00 9000000 00000000000 00000000000 BD O II 000 00 000 000 III OUUU UI L s bNADRNAL UD DU DU D U OOOO gut bmumuuuumuiuubiiuvuuuiiliiuiu s DpIDnDDBImiiduiiubiuipubiu uiu ut s s DDODBDDUDDBIBDUDUDLUUUDDLDULLDUULUDLuUmLL 000 Jt ti eiillilliliiL l O OO O Bp ubl ubi uupiidabuui pii 000000000 UUBDBBID dug DB d Hl V 00000000D JOUDU L 0D00000000000 0000 00 000 0000000 00000000000000 00000 0000 00000000 s DDIDBDSOIDBUEBULDOUDULDBBBIDmbuLbiLm iL s pDpaggigilial Bp p BOB UOLUUO U0 Lg i ul s U G OOO 00O UGE dg s popali gll lt DU OO DOUO OOO CUOCO 00000 0000000 e uil iuiuLululutl s BUB D UD L D D IU UDNALTET D UO UO OBL O D EO D BEL OE II L PO 00 00 0000000 DU ggpiiudiiuiuiiii biitut 10 000000 JULI II l D D UD 1 DI 000000 00
6. p Om Uu Bm iBBlbm BEI BL BTE ET D D RR RUPEE IE LET EI ET E 3 3 3 CJ 3 C3 ELTE r3 r3 3 3 E J J J UU D Buffer Buffer AVL Carrier BL E EE E E D D H EE E D ET LEE EI 00000 CAMP O EE D 00600 ELE ETE UU U H O O O O0 00000000D o me a HI T Looparp 0 000000 00000000 00010 100 L e 1 10000 000 054 LI 00000000D J nHHHBnonmgg p mmn ng n BEBO 2041 O O 00000000 006200 20001 5E 00000 0 000000 0002000 1000 1 O 0 0 O Oo agaung gmnuun mnnnungunnnnmuna nunnngnnmmnnn nnn nnpnnnnnn O 151000000 Oo o EE on O Leemn nunungnng nnunnuun D 5 E 0000000 O O ngunmmunnmununmumuunnnnn nngmnnnmnnnn O o 3 000000000000000 0000114000 EE n E pHgunnngmnnnnpngmunnmnnpnnn 0000 elllli i iii II 1 0000000000 000D 0000000 19 0 Ug guit Lt s 601 1 0004 000
7. 02 55 57 uguali mulu t t OOOD 000000000 9 553000 g D lt oH O D D DNA RNA O OOOOO O O O OU 0 O 0 00 0 0000000 00 JULI IU do d PTPP EH EJ EH ES C Lig E A a g Notonh T et al Nxleic Acids Research 28 12 31 2000 Nagamine K et al din Chem 47 9 1742 1743 2001 0 ugu u uaiumnauauuuuuliuulutltluttltltLtuttL 2000 Mri Y et al Biochem Biophys Res 289 1 150 154 2001 00 00 O 0261 0000000000000000000000 2003 Katayana K et al V rol 299 225 239 2002 uu 00 0 2004 OOO0OOOOOOOOOOOOOOCOCOOOOOCOCOOD 54 N 3 667 7150 1999 Asl anzadeh J Annals of Cinical Laboratory Sci ence 34 4 389 398 2004 Prince AM Et al Bi otechni ques 12 No 358 360 1992 0000 AE 98 103 Ugaguu ul uit LUUD DBLL 2x Reaction Mix NV Primer Mix N Enzyrre Du dguuuauiuluilliuni xsiuuuini utrum utu
8. EM Lb nm J 1 Eira ra m la m m g la m m m g 11 L He uuuiluiiltitiliutLuLtL LL l D UUDBUdiIIHDU LLL a r3caca r3pQ 2 HEgEPHBurapmmp amm c c c c c c c c c c c c c L c c L c c c c A L D UD duum ase RNase 1D OOO O 00000000 DNase RNase 1E 0 OUO asd RNase 0 000 0 0000000000 E j 0o00 1 00000 LAMPO D S5666660606060006660606000666660606060666060606066606060060666606006666060000666600006606000 UO OUO BEIDE ETE DEBT D C 501540501440 50440 04040 4 50 4 0440594 0 0 4 0
9. 5 igopome p mediated Isothermal memo method i TUTTI TRE i E 22 i Ms 1 t3 E opamp JU eut opamp gU ddutMt OODD UL AMPO 2000000 A L cue LERF Lzugr medistesd Inattiemrat methizst Z EJ gis D HHBEUHHHM P 2 s P 3 i P 4 II 00000000000 5 V 8 V ree P 11 EIl P 12 E DHHJHHDHHHEHBg 5 P 13 I EU eee P 14 II P 14 2 15 V HOOI eee P 16 M P 17 P 19 20 00000000000000 Hui eem n mem eim gun LLAMPE ETT CEU EE ULUEET LLLI CONTENTS LU LI LI g L LERF Lin medie Inaftiearat Sangl ficatiaa mgthinst i 4 ug rr I 00000 LI EE EI LE T e 20 3000000000000000 000000 0000000 000000000000000 20047 x 0000000000000
10. 1 325 o0 g2 mn r3 oad 107 L3 amp ooo AHA Cr E oog E33 Ood F h L3 gH L ooga p 15 LJ H 1895 O m a 5 oog an0 4 Eh ym EE c c q oog 05 250010 0 5 mE a goo a gt gjan aii Lo moog Tuer e e e r4 O L3 3 3 L1 LC O L3 L Ir L 1 1 r3 3 H E O O O 3 H rca ocg i m EH L3 L pL oaao H rir eed p4 L0 r3 LE gaong mium pac Hoo dua r i aua 251001 EH 0 01 m e oogmpgadssooB m C lt 12 000000 br bip O ESE 5 ganl AAAI d Zu 11111 a af vais qut uuu built OUO 00000000 BUB i D DULL O LEID CD DEAMPT 00000 ii 7 LA 3
11. 3 2000 mr o E E H ET D gt p rur ES O J FJ 71 1 LJ c1 x H L3 Lr 3c c 230 Hp H HS 20 p 1 gt 1022 ri Oo mBa n 2062 22 Hdg N 3 EJ A 1 c r3 zi g2 BE x X 1 r3 g g 1 L3 d a a gY Lc gt Lr LL E LN 3 3 H tL H LJ ogo L3 r3 Ln OcA 12 5 L1 p H O oga A r3 Ld I1 r3 Z 1 L1 ELS L I Ogag LL LIL 1 L1 5 Cg o o RS EGEH go GED ri E oA g CI L 8m rc ozgan 5805 gu g do czESECO gt gt LL pL mp L3 5 uu g Ogopa par ct Cp Aon Og Z fe L3 3 L3 COE c zL Omm Lc 2g Ocna 609 2 1 r3 pig r r3 2105 505 1 6 x cu te L3 x B4 23 m HS 1 s Hg BHg gBH L3 rp cca L3 pH lt CJ eaa ngo 20g m i cp E d 2 8 SEES C o0 rn P E uri EE EJ EE goo ri o 53 r3 02 1 amp L3 E34 r4 ang 2 02 05 WO
12. oDnDLubiuliul inu luu s 00000000000 e JI 0000000 s D HB s s 000000080 000000000 OOO L s s 8100000 00000000000000 D UBL D Ungu iut s UD IU LLL l III I III Il P 19 wis EE o ili nunnn nunnunun mumumummuuginnuuuuunuunnni nunnnunnn I DO LDLE DEO ELO LEE U LI 8000 0 l SU LAMPO 000000 1 HL ELE CURL HL I D EL BLU FOE ELE D UI UI E 1224 em p E H E e LJ m EE og o mj a a r3 CJA M gt c jin 2l co NA p C 2 E E C E mapu c 1 L1 C 1 L3 r103 Li L1 L1 L1 1 1 c4 Lr L1 mm Lm EMT T g gog L3 agp zem E o L2 03 n3 E Ei Dm gg 1 5 L4 oo m C L1 gH 1 L3 r3 L3 H 5 o 3 5 3 L3 LJ 1 g Aa g m 3 r3 L3 r3 L3 L tL 3 PE ES L3 Lo CL mn r1 LH rm ETE L3 r3 oN r3 L3 pc ESI 3 r3 05 O0 o0 BB a a um 02 1 E BS cm 2 5 Dm C3 oog
13. 1 0000020000000000 00 000000000 pied D0000 000000000000000000000000000000000000000000000 0000 e 00000 0800000061 00000000 Genogroup GI genome RNADOOOCCO 0800000 061 D D D 0 0000 Genogroupll 00061 0000000 UU 00 O L s JUDI O genore RNAO O 00 000 0000 D BOCbNATDE EL O D ORTO O BECDNAT BD EE EE U 110 0000 0000000 RT LAMPO D D BUD U 00000 Genogoupi GI s H0000 00 0 0000000000 0006 met mnmduuuuuttuiuuiull uuu gggguuuu I III 0000 000000 NID III 0000000000 000000 s RT LAMP Reverse Transcription Loop mediated Isotherrral Arrplification 0 LAMP 00 0 000
14. D UB p E D 00000000000 1000000 IU L nnnnunnng 1 V BL ELE UE D ET DU l JULI I LIL L Lj 800 00000000 Distilled Water Positi Control daad aad ETE ETE LL G LL nr rr nnnna Lo mumnnnnngo Iunnnnnnnmnnitsssspaspnagn _ IV LELELE EE ELE ET DE LI D Jt uuiiiulbulL tli TRUE a AEAT ATRE ERE E EE HM E 0 550 s JULI LII D s Ip dbi b BUMI II t di L s 0000000000 uidbibogilgbi gH BUD dil l O 000000000 000000 000000 000000000 s DD D DNACRNAD 0 0 DD D 0 000000 Mire JULI III 000 000 0000 s UVOO00O00O00000000000000000 0000 0 s DO0000000uv000000000000000 TEE 000 0 000000 eilullulll s 00000000000 0000000 000000000000 0009
15. EE DO Buffer AVE L 0000000 6 000x giliu O0 00000 O LL L1 ume y pmi eenn my fer jennnut Enzyre Mx EM 2x Reaction Mx N 12 5u L RM N Pri Mx 2 5uL i stil ed 4 Ou L DW Enzyre Mx EM r E Ez m s n gmmgdu 2x Reaction Mix NV amp Gl amp Gll sk Primer Mix GII Distilled Water Gl GII 000000 95 5 0000000 000500 1 Enzyme GI 01 Loopamp T 00000 d nono n m uude ttutliibilL Y III L UI U I 00000000 5uL Positive Control NVG1 PC NVG1 H NVG2 PC NVG2 i 00000000 5u L Distilled Water 0 4 00000000 LAMPOOO 2501 con S Loopamp LARP Lanp medisted da meti i TALL E Li E 2 J L1 L1 L1 L1 1 Ex L1 L1 L1 L1 L1 L1 L1 L1 L1 L1 ps q ELE UL UUD UU UDU GOA L3 Dm Ll c H O F4 17 3 E I
16. PM NVGII T 1 Ol2mL x 1tube I3 Distilled Water DWT 1 10 x ltube Enzyme Mix 112 60u L x ltube Positive Control NVG1 PC 1 x 4810 00 2x Reaction NV RM 1 Primer NVG2 JPM NVG2 1 0 12rrL x ltube 3 Distilled Water DW 1 10 x ltube Enzyme Mix EMT 112 60u L x 30LAMPO Positive Control NVG2OPC 1 20000000000 0000000000 2 0 5 00001 0000000 2165 DNA Polyrrerasd AMV reverse transcriptase 0000 00 euim 3 EB IILI LI E E 2x Reaction Mix NV Primer Mix NVG1 NVG2 Distilled Water Enzyrre Mix 0000000 Distilled DW Positive Control NVG2 LO m Uu NLD l SUN XXX rrr rr unnnn IW rn pr BLU EL BLU D CODI EI E uuu L e UUUUUuuututiurm9Aamp Vira RNA Mini Kit TT UU DID OUBUdDdubDtiuiuuiuLbubilittiutuuL tul
17. lt L 3 3 L3 r3 p O 5 3 L4 EE E eO nmm pcr prr zi LE pj 2000000 r3 LL L e p 515 Ql aga A ag A a Al a A caa A 1179 amp ojm H am
18. og c3 ogn oH 5 2000p o e L1 a 2 iL 252214 oop c ouggoaumagdogdggaa aug 1 gs 2 LJ L 525205 5 i C31 Cm L3 c3 0 mH 5g Ph 222 p H5 aaa g p 2 HA e HmpnHm D uu C cm 109 E gt Far mpm m r3 amp mro gga C L C303 E E83 03 tte ao 0am O Ga A Og L3 aA L epp ura 0269 ai aaa L 1 a 2102 050 05010 g g Heo e eoo 144 He e He e e zu IV LAMPO D DLE DD ungngnang LAMPO O gggggggggggnn VUUUH i U r E 1 E O lt b L1 B SEES n 8 ES BS go O r3 3 o O To 2 um nuu H p 0 pu ES E pa r3 o got r3 aA OA guum O no pp Ono 2 0 Dm O O EE L3 L3 cH t g L3 prb ag 1 o gadd L L1 A L3 r3 Lg L3 E A ss B Lc 2 a P s 2 270 Ege SENS EE Lar i i ratna nagd 220 a ii 170214 L3 Lr 1 ELE
19. 0000 000000 000000 000000 Loopamp LERF Lexugr yxediztes baena metha j 1 1 pk a K RTA ESTETISTE T IR E r r1 T T T nnnuonnnnnnung I AusrT F T T T 3a 0000000000000 91 Viral Mini LE EL E BL E BUDE EE BLEE UL EI 1 L L1 t 1 5m 00 0 200 p m Baa 00 000 O Buffer AVL Carrier 560u LO E 000000000 O 140p LODO O 00060 Buffer AVL Carrier RNA 0 H 0 0000000600 uguuguulutgudatuauggduduttiuttuttu 000 00000000 101 00000 0 000000 000000 BUB ugpBatugduultutubbti LEE D 198 1001 T 560g LO O 0000 151 000 0 00000 0 00000 L U L UJ H 630u p 2 00000 00 OOO OAU DE DLE HE ELT 0000000000000 0000 000000 0 06 000 111000000000 OO0OO0ODOOODO OO OOOO OUO OKEE I Bi E uU genres LI 0 e0000 uu Buffer AW1 5004 LODO 0 0000 000000 0 06 000 411000000000 up 0000 00000 00000000 oU Uggggilubiidgibbiil Lt HO OO UU U Buffer 560g LO O0 0000 0000 00 0 020 000 4431 000000000 ub 0000 00000 00000000 O 0 0 00A OOU ODO 020 000
20. 00000000 201 2000 L o 200 LOO0u L tut utu aka 1 0000 0000 000 0000000 0000000000 MSrhii 20 0000000000 8 O5 15 Jp Bm am ut uu 0000 Hi UB D DULL 00000000 O00000 O OUL 00852100000 Lu m D UL LU rj A s et 00000 000000000 C s 000000000 L LAMPO I 0 00120 000 O DD UD L3 r4 L1 c3 9 Der rl 0 00 0000000 s 0000000000 0000 DU UOOaii iul Lt IH 0 50 1 5m0 o ngu s 000000000 III UU L s ILHUOBUBOBUDLULDLUL LU e 0 00000000L s 0000000000000000 utut 000000000 00000000 s DU DI s amp 00000000000000000000 09000010 s III III CTELD L 0 s 000000 DDULL s 1 000000000 0OOD UJU 19 00000000000 s J LAMPII 000000000 amp rmimbltut Lm s D DOUODBUU UI U U D UU UC MW s 000000000 OOOO tL oDiiBiut ii ibi O A
21. 20C 14 nml i 0 il V 0000 FA oogoHgagaHooga ES DAbs III 0 14 L 320 UL BUB i pi DUO O DL UO UD U CEI UI CTI o0 r3 L4 L1 LH oa g so 49 Oo gO lis 11 H 3 L1 r3 H A 3 oa m o5 um oO Ha m Ei rip 2g O r3 L3 r3 Og L3 a Z E A r3 L3 L3 r3 H 0g 4 L3 a A ag H o a L3 d p EJ A E pue 5 j 15 DELI LU LI D 160 L AL D 1 IUIN YNY IEA rr 7098009202298 rrr PAKETTIEN rr r r 00 BIBOU BLUE DI 0 15m0 0000000 BufferAVL Carrier RNA 560 LO D C 00m0 00000140 L 0000000 pnnnnnn 0000 n 960 1000 0560 L 2 0 000000000000000 esour O OOOO 630p L oj 6 000x 9010 0 12 LI 000 00000000 000000 L L L L L L L L L L L Buffer AW1 500 L 6 000 gf D LBLBILBE BIBLE BELEE EE EE EE BILE Buffer AW2 50091 20 000 90300 EE DICE IU L 2 20 000 90100 L DLOUBOUBEBB UU DELOL ELE BL
22. 9 9 0 5 4 0 0 0 0 0 4 0 0 gocce 555555555 25555555 oC 2 coeno poo 55665550 5555 995455555050 ILU x pO 009 55 555555555555 050545255555 255555505096 SS eS eS eS Seo Seo S OO Oeo I oe eoe oe 9000009 NS eoo VEEE posee 295626 p RR RI II RR UID 55555909792 Se So pe 90 90909090905 5555555 SESA 55555555 525625055050 555550452555 566450552505 5 550465555555 aaO 555555 255555045555 S Coo eso pore 606455556556 5555555 41 NA o i t tI 909 00 oo md m 670107006 767676 52152524 rec o 506 877474 Mes soo EPUM ia i e L1 55225 E 5955 d OCS 2524 5555525 9292929294 eS EE 5555 5555 k E J 52545 OAE E LE 505050505059 SiS REESE COE E Koc dece 1 dp Ep E dooodo been qiio KOETA OAOA
23. O0 g OQ I 1 O O ra m lt 5 500 55 0000000 00000000000 Unum papi ui 1 1 Ez 1 1 1 1 1 E L 1 1 1 1 1 1 Ez 1 1 1 g a aao ad Ad g A 1001 400 3150 V B 0 280 315nmj UV C 100 260nm D 00 00 000 UvO UV CL I 000 D 249 280nni 0 Dial L1 1 1 3 L L L L3 mr m Ho ma SS O CJ C L r4 L E L1 Oa rE m o gt 1 g2 ma 3n 1 O n 3 EE g E 1 L3 Dm 1 1 C 1 0 15 L3 r3 r3 L3 O o 3 a c L m Ei O o 43 p oO z0 n C O c 51 51 c oa 3 EJ Og m LO c 1 UH LU HOD DELBL ELE UICE D LU UBL BDB UU LIU LU LH 0 tutt UgpuiiluiutuLtiiut tuli ES ES adig EET E
24. RO sd ooo Xe X E224 T 6 L 56855 226 52664 oco EEA LJ LLLI pL L 2555 E 2 555555242 152525505554 242 22225 E oa 155525556555 1 L 12221 1 tat d L1 L r 4T1 L gt E L II Jl JGJ 55554 o l rr 255552950755 Soo Seo o9 9990909 90905 1 IESIRE eroi NONU AT 9 90 4 L l mena CJ 1 800050 06 0000740 L L j EE 22452 eee 4952 0 X 90 24 1555 Ll li 555552550555 550955 Mt Ten iate to totae att ato LL B LI BoUDUBODBBBOHBHBPBEBEOULBHLELLLL rF 1 r 4 d L Robe 4555 52 E E 557 301 4767645 09 95 25 29 9 Qi ra esti io e e md e ee ool L 225555555645 EJ 9 06 ot t t t t A NNA epi LE EJ OOODOODOIDOXOXOIDOIXOOCKO 4 os coL E L L h M A L 3 L L TTL IL di Ww Ww Ww E E 1 L L L klo 5 SOS A L L OO 25525552555 2555265555055055550550 50 0 0 0 00 5 0
25. e 0g E Cj 2 r3 D13rQ lt O O O o 3 NAZA z L3 E L3 a ELI m Ea 1 L3 s gt 409 Cm m3 L3 3 L lt Lr rcg 3 r3 C ogo r3 lt oo 2021 05 21011 3 2 0005 r3 ooo Ooo r3 L3 Onga mm LP d OSE m 26 L3 r3 H reum L3 a r3 CI Lr Lr LLL L1 lt Oo E i EE E Ln L3 15 400 O aHa 5 L2 pm r3 cpm H g Oog EE C3 nmm J 11 n LJ O00 Sij L3 rcc od ES Cii a a rc E L rc LL 1 014 mm um n Fm E r3 302 DICE 5 2 d 3 C38 2 gcc Bragg us 0210298 um magno or o720 Hug I 1 ES iii i mo O pu unusm8 ui xx 2505051 29 Oooo gt o Hn HE L3c3 gt 00 42545 5 aW 509051 az a Z E c3gcacicacsHesgainmemeadaaoaomaeoBaaom9mm os p000 rc rc o2 aQ 2 a r aIa z z BE g LrJrs 8
26. g 010 000 0000D L O 0000000000 00 15 BOT Buffer AVE 0 6 000x g O10 0 0000000 l D um ggaudibliubiuitulutiiublili iu 000 RNAD D DO D D 208 00 O 70 9 0 O O UD LU 1 0000000 En ngmunungunl unn uuunununnnni wW l 2 Reacti on 12 5u L 12 5u Lx 69e 9 gt 0151 Enzyne M x EM 1 Ou L 1 0u Lx 901 50 Enzyne M x 1001 1001 inu L Pri Mx NG 2 5u L 25uL x PM i 00 Li still ed 4 Ou L 4 0u Lx un 1 2 Reacti M x 12 5uL 12 5u Lx RM d spur Pri Mx 2 5u L 25uL x 00 amane M Aut nb L3 3 ogo r4 L3 o0 Lr E m 6 cin pz r3 gt L3 um gog g rcr po og g HA O mjm comma oA Fr E ESO SEIEN T Lo a IZo L3 9 e OO o amu m9 mu p 0 lt ODV Soo g EE BH pg 09 2 pum um n m t Lc o o L3 oo 3 L3
27. rn 7 Oone ED IILI L s s iul s DDDHU ODUBIBUBIBIBBBUUUUUOUDUUUUULLDLUL LU eilluiiiiuii tluiiiut s DDDHIBDUOUDULDDULDDLUEDDIDLULOLUL BODL U enu 1 s DDDHUBDUOUDULD DU DU C B DELE O EB EE DC BEL O DCECELCE L UL s DDODDBODEOLDDUDBIUL LU a U I s e ull ti DUULDLUOOOLUO LB DOO DEO UO D DEO CEU OCGEO E OE IU E Ul s DU B D ELE CDD EE OE CO DEO CELL DT euliulllli s Jt 0 s pDpmpopiupiDudgudiutuubibii utbblt ubtuiupiiututul 0 OUO O 0000000 l s LL LIT 000 O 00000A uuu 0O00
28. t LU pO BOOBUDB D ELELO D 000 L1 DH 0 0 IEnzyme LAMPO 0000000 000000000 00000 000000000000 020060 O 00 LI EI 000000 00000 2006 00 000 4 3 L1 1 8 5 o L3 En G ET ET m L3 ee L1 O o n m m Z o O Ia g L3 r3 1 d mi o 8 o H g v O o B g A wi E C 1 1 1 1 1 LL LH EJ gt i m 9 Oo m r3 O r3 Hog Oo Ogag o o g L3 3 m EL 3 dr E Sh L1 o O o O 3 m x E EP Ej A L3 L3 2o00 O g rr LL p H E O00 o nm Sc EN o7 nun r3 a 15 o m k a s 21 3 co L g r3 oR B H5 Ug t H r3 L3 Oo 52 O ri L5 L3 rir r3 L3 L3 11 o0 A 3 rH g L3 oA OO 2950 E E L3 r4 I HOOOHOO Ono a Hga c L1 LJ ug CO i l o 0205 ia H comb mg Oog H r i B cn p an _ um r3 L3 m LIE Do m 0 m fa D n a Lm L3 C a c H Og
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