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Contents
1. NAmrvsN NmivsN EP Time2. SDS PAGE Native PAGE NATIVENeO PAGE SDS PAGE Native PAGE DNA etc Ot NATIVEN NmysN NuxrrvsN
3. 4mL 1 mL 4 NNAmrvsN A B 03 SDS PAGE gt 9 8 5 gt sy 01 05 11 2 0 cm 15 450pg Se ini EP Time 5min 16 9 se ee 14 7
4. TT NAA RAR NSSSSR RD NN SSSSSR NN SSSSSNN ST NATIVEN NavivgN 3 NATIVEN NmrvsN 5 4 5 03 01 PAGE PAGE kDa NAmvsN 3 1 2
5. CLuc NATIVEN EP Ti 5min WesternBlot 4 6 8 10 12 14 16 18 No No 2 4 6 8 10 12 14 Ref WB m 06 05 NaAmrysN 02 Mass Spectrometry MS Nariven SDS PAGE 578 9 NL Native PAGE 100 6578 o DAER
6. run time 1 99 collection time 1 255 fill time 1 255 max fraction 1 999 5 65 400 mm x 250 mm x 200 mm 2kg NmvpNe x 1 x 1 8 amp x 1 x 1 S S x 1 L S x 1 S x 1 L x 1 S x 1 L x 1 S x 1 L x 1 S x 1 L x 1 08 ATTO Preparative Electrophoresis System AC 5700P AE 6760 NArmveN AE 1650 PD Nx
7. Fill Collection Pause 4 m EP Time 29 90 20 ER 16 40 10 35 7 13 a 12 5 20 0 7 5 12 5 5 0 7 5 Reference 1 Separation and structural analysis of lipoprotein in a lipopolysaccharide preparation from Porphyromonas gingivalis Hashimoto M et al Int Immunol 2004 Oct 16 10 1431 7 DOI 10 1093 intimm dxh146 2 Saccharomyces cerevisiae Rot1p Is an ER Localized Membrane Protein That May Function with BiP Kar2p in Protein Folding Takeuchi M et al J Biochem 2006 Mar 139 3 597 605 DOI 10 1093 jb mvj063 3 Synthesis of glycopeptide dendrimer by a convergent method Ozawa C et al Tetrahedron 2007 Jul 63 39 9685 9690 DOI 10 1016 j tet 2007 07 023 02 NATIVEN NAmrvEN OS NemvexeO NaTIVEN
8. 3 EP Iime i 220 170 116 _ 100kDa 0 gt 95kDa 7 5 53 kDa o7 68 120500 Rin KPa es 20 14 kDa 5 YR kDa 04 A B NmysNe NerrvsN
9. 2 09 AE 6760 AB 1650 3521215 PP AB 1650 AC 5700P 1281500 AC 5750 1292101 AE 5700P AE 5750 WSE 1100M 2321640 R WSE 1100MW 2321641 R WSE 1100 AE 6530M 2321900 AE 6530MW 2321915 AE 6530M AE 6530MW 1mm AE 6401 2393010 2 2311165 500 2311145 AE 8155 WSE 3200 10 LL Catalog Ver 1 0 20130208 5F AIT TD DNA 111 0041 3 2 2 03 5827 4861 03 5827 6647 03 5827 4873
10. 03 5827 4874 110 0016 2 21 6 03 5818 7560 03 5818 7563 E gt 530 0044 2 8 1 06 6136 1421 06 6356 3625 URL http www atto co jp e mail info atto co jp
11. ATTO Preparative Electrophoresis System AE 6760 NATIVEN Since 1964 aL 50 RU SupportingLifeResearch AE 6760 NATIVEN AE 6760 NATivgN Bio S 2009 9 NATrvgN PAGE polyacrylamide gel electrophoresis PAGE AE 6760 NATIVEIN NATIVEN
12. 14 000 1 SDS MS etc 1 SDS lt SDS Sol A 2mL 1 Sol B 2mL 1 Sol A KCI Tris OK Sol B Tris 95 SDS Sol A 40mL SDS Sol B 10mL 1mL 1 04 Native PAGE Cypridina Luciferase CLuc Native PAGE CLuc
13. 10 7 uo 8 2 6 2 Y 2 5 kDa No 05 SDS AE 1390 DS e zzz777 SDS SDS No 05 50mM HEPES SuL well 1ug uL SuL well SDS No 05 SuL well SDS No 05 SuL well 6 a 60 80 100 units uL Micrococcus lysodeikticus A450 1unit 1 Micrococcus lysodeikticus A450 0 001 1 A450 unit SDS No 05 12 05 CR SDS AE 1390 SDS eliminant SDS SDS PAGE SDS SDS lt
14. Base Peak F FTMS p NSI SDS 80 Full ms 400 00 70 428 3 637 9 1600 00 MS attotest gt 60 600 3 a 8 612 3 50 40 I sse 3 1038 5 30 din gel digestion 673 3 5998 ST 1 920 3 10 553 8 413 2 Y i NI A h J 1 Mi MT 961 9 0 5 10 15 20 25 30 35 40 45 50 55 60 Time min 06 DNA X174 Hinc IT DNA NArrysN 6 0 cm 7 5 INATIVEN EP Time 3min NAmvsNS 07 gm 2 0m TO mm io xm 50ma 9 Le M NI
15. nD gl SS 6 0 169 33mm2 lt 200mm2 200mm lt 1000mm lt a 7 5mm 16mm 7 5 17mm 16 37mm 6 240mk 500ml WA SS Es 100 300 100 lt 300mL mL 1 40 mA 15 0 a MAKE 400ms LED 4 x 20 LED ED E9 8 S 4 1 40 mA 15 delay time 1 999
16. mvsN AC 5750 A T AEP 560 AEP 561 AEP 56 2 AEP 563 AB 1650 P I D 20 80 PID AC 5700P NATIVEN 48 e AC 5750 50mL 2 NrrvpN 48
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