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        ALR-102
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1.                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     Q UTP                                                                                                                               Q UTP                                                                                                                                                    RNA polymerase                                                            T7 RNA polymerase  881767 Roche      T3 RNA polymerase  1031163 Roche      SP6 RNA polymerase  810274 Roche    10 x Buffer RNA polymerase            Buffer   RNa
2.                                                                                                                                                                       Q UTP             DNA  In vitro    PE     RNA         Q UTP                 C s     RNA                                                          UTP   NTP              ALR 102                                                                    RNA             TGase            RNA                                                                                                                              ALR 103     TGase                                                            TTP                                                                                                                                                                                                 5                                                                       ATP 20 mM 20 pL  CTP 20 mM 20 pL  GTP 20 mM 20 pL  UTP 10 mM 40 pL  Q UTP 10 mM 16 LL                       1                     RNA polymerase                                                                                 2  UTP   Q UTP                10 mM                                                     20                                                                                                                                                                                                                                                                             
3.                                                                                                      Q UTP                                                                                                                                Q UTP                                                                            3                                                                                                                                                                                                                                                                             ALR 108                                                                  400 ng                                                     Q UTP                            in vitro                          Q UTP                                                                                                            RNA                                                                          PCR                                    UTP     Q UTP                              1 kbp                in vitro                                                                                                                                  Mi                             100 bp  1 UTP Q UTP   90   10  2 UTP Q UTP   80   20  3 UTP Q UTP   60   40  4 UTP Q UTP   40   60    1 4  T7 RNA polymerase                20101203  RN1 3762Rev0                                                                                            
4.  RNA         in witro                    in vitro                                                                                                                           G 50 RNA   Roche                                                                                                                                                                                                                                   40C   2                                                                                              ALR 108                                           Stop solution     4 LL           TGase                                                                      Hybridization Buffer                                                     42   70C   30                 Hybridization Buffer 9                                                                                                    10 ng mL   42    70C                                                          Wash 1 Buffer 2             42   70         10             2  1        Wash 2 Buffer                       5            2                            1  Hybridization Buffer     2 M Urea  20 mM phosphate buffer  pH8 0     0 5 M NaCl  1 mg mL torula yeast RNA    1x Denhardt s solution  1  Casein  0 1  Triton X 100   5  Dextran sulfate  MW  500 000     2  Wash 1 Buffer    50 mM phosphate buffer  pH8 0   150 mM NaCl   0 1  Triton XX 100    3  Wash 2 Buffer    100 mM NaC1l  100 mM Tris HC1  pH9 5    50 mM MgCls  0 1  Trito
5. ALOKA  Labelling ONE             20101203  RN1 3762Rev0    RNA                                       UTP   NTP                                 ALR 102                                                                         2                                                                                                                                                                       Q                        RNA                                                                                                                Q UTP                                                                   UTP              857                                 RNA polymerase                 RNA                                               Q UTP                          RNA                                                                 ALR 103                                                                                                                                                        20C    1                                                                                                                                                                                                                                                 20C                                                                                                    6                                          20C                                                                                                           
6. n X 100                                                                                                                                                                                                                                                                                                                                                                                                                                   181 8622                               6        22     1                                       0422 45 5181  http   www aloka co jp   e mail  bio aloka co p             
7. se inhibitor RNaseOUT  10777 019 invitrogen    Water                             PCR                                                                                                                                                                 G 50  RNA    1274015 Roche      mini Quick Spin RNA Columns                            G 50  1814427 Roche                                                                                                                                                                                                                                    1  in wtro                                PCR                                                                                                                                                                      PCR                                                                                                                         87         2                                                                                               10 x Buffer ET                        DNA 200 ng   10ng uL  RNase inhibitor  40 U L  1L 2U0 L  ATP  20 mM  1       1mM  CTP  20 mM  1L   1mM  GTP  20 mM  1      1mM  UTP  10 mM  12pL   06mM  Q UTP  10 mM  08pL 04mM  RNA polymerase 2p   40U           Water to 20 pL                         UTP     Q UTP                                               UTP  Q UTP   60 40                                            2                                                                   
    
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