Realtime PCR Master Mix
Contents
1. LightCycler Idaho Technology Inc Roche Molecular Systems Inc TaqMan Roche Molecular Systems Inc ABI PRISM The Perkin Elmer Corporation EEL TL E Li O mm n u aa nm 1 j j LI Zi HH 1 Jl l H1 DZ n n 1 D 1 n l Hl i Hl D D DH DZ ERE ET N DILE BI LEN E El LI oo DD BD o oo SSeS EDE JE Ez En n dn 1 D1 O o o Seeley TE CI Dl Z2 HHD Dn DI DI Dn J l Hl H1 oo oO n J Hi DI j oo Co ono Os nm A Gi EJ Zo 00 o0 o o pgpo o0 ono DI ee opoSblo 25 DI D DI e 0O gn oo Hm EH HED 8 mn oOo oo DD sE SEFHE Do Bee a mn YOR gH HH o DH A a n D o n 5 2 oO n Ho H DI nn o oo oH Ey Ej ea Seo HH o ETO n ELE n 2 ER pol0025 3 Hm g n c H Si Udo o Does Oo n dn o pa SN Am ooo o Se SS E TEVA NEB foe ho YOO goo oo a SS nm st O a DI CD n D i 0 n z bo ogo D n Dn D D n j D oo Oooo D 1 soo Oo EVET LETE ae Gem ELE nm L Jl i J j BD ao m DD b ID DR on p2999 HBD DD DD DD ooon n DD u n 1 1 Hl Hl 1l cio o GRE N HEN B N VER HE BR NT E Realtime PCR Master Mix OOOO0PCROO O P2. DOO TaqMan Q OOO0O0000000000000000 UOUUOUUODUU 1 li li ll j jl ReverTra Ace Code TRT 10 1 Q0000000000000o a nm Pr ooo mn 1000 UUUU UUUU 1 a O0 O RNase Inhibitor Code SIN 101 102 0 5U u LO 0 5U U LOCO i i j j ll Bi li i i i ji i fi i Ll li i j 00000MU4 L j LJ Ii i Ii RevertTra n HRH 1 OUOOUUODUUWHO Ace 1000000 ReverTra Aces 0 0 O GRNase Inhibitor 0 0 ReverTra Ace g 0 0 oon U U Ll B B J j ji li ji SU H LO OOO W OOORTOO0O RT Ooo Ooo U U U Oo Ooo Ooo Ooo UUU OUU UUU ILIHLEHEH HH HDH j 0000 DNALI Hi ji ji l1 l DNALH i Ji TO l1 B 1 1 1 J I1 Bi IJ RT J I1 li Ii 0 DO0000000000000 000000000000000 RTO 0 0 0 J ReverTra Ace 3U u L 0 O 0 3U L RTO O O O RNase Inhibitor 5U H L 32 uL ReverTra Ace 100U u L 1 HL UOUU 33 WL PCRO O O 0 TaqMan 0 000 j j li 9 UL Realtime PCR Master Mix 25 UL 00000 1 10u M 2 uL 00000 2 10u M 2 uL TaqMans OO 0 5u M 2 i L RTO O O ReverTra Ace 3U y L 5 uL DUUUUDO SUL UOUU 50 WL O Realtime PCR Master Mixz 0000012000000000000000000 D0000000000000000000 000000 0000000000 DUOUUDO000D00000UUUO D0000000000000000000000000000000000000 DOU000000000000000000000000000000UUU0O UUUUUUUUUO0OO0O0OO0OOO0OUUUOUUUU000D0 0 U N LD TOR cu nln gog Ooo Hong Li mn DU CO oo a DU Co oo mn DU Co oo n i i amp Go n
3. i i amp Go n Bl H n n Er Em E E Pi E TE D c Hi n D Hi n HH KI EN mm z jl l j j RD CC O oO nm i j HT RD nu i i il Hl Hl H1 N UJ U Dj D DI CD lt OF Co ABI PRISM 7700 42 C 20 95 C 50 nnnn 60 C 60 data collection 95 C 15 HOHUHUOUUUOURUOY N ELL N HL LEL NL TI M L DI DI DI Oy nm Dn CI DH g DI Oo ID Du DI Cl jC Du DI C4 Co hm ao m Si o Dn Cpu n am m asa Ez C4 Co CI n H DI C4 Co D n n H n olo m Dn lt D ml mn ml n EA I D ologo ooo n Ic lalo glao ooo om ol fo ama ooo r fo malo ooo n lo lalo glao Serco n ln lalo agao ooo E ol lo ml nln olo ooo mh nl n nl n cla ooo clolo olo ooo mn D D Jo Ez ooo m malo oO oO UH mac lo Clo H o mn mn o mn H mado al H oO Hm ol Ney HI In nl H n oO o jo HI o 4 mado al H oO nm jo HI In H mala al H oO HI H n ninlmn al H n Cla fa HI H mado al H mn Cl nl lo HI n og ola nl H O go DI D nm nl fo oO D gojo si oO o o ST Oo rm Sala amp 0000000 oo TT Hi Code ReverTra Ace a 9 1000 0 FSK 101 ReverTra Ace 10 000Ux 10 50 000Ux 1 TRT 101 TRT 102 RNase Inhibitor 2 500Ux 1 2 500Ux 50 SIN 101 SIN 102
4. As nz i l c u 1 a n PZP oo n 1 1 j 1 1 DI p2900_Daq8380 nm H 003 mn n Zl c i 1 1 n 5 D DD DI po Zn2 z DOLO DI 5 H HH 3 i ZI 0 nm Hi 1 H ons mn z2 Li DJ h i DH Dj o n i l H a mn REN EE E 7 ooo nm i 1 H Hl i H1 DI DU Tl 6 ll l foo n ll 1l j 1 i 1 o ET en ED EDET EE n amp j n o0 oO po ET ET rn es n H D 2 Q Di i i i 1 Jl 1 H1 en El es E Oo n n l Hl J j j o D 1 sooo O HH z lt nongoa o Io Vv oc DI PCRL 000 0 0 00000000000 60 95 C 600 x400 000 60 C 60 data collection 95 C 150 5 6 HL uL 0 6 uL 0 6 uL 0 8 uL 0 4 uL uL uL 10 2 20 UUUU UU 0 D Roche LightCycler O OOOOOO00000000000000 W000000 PGCRL 0 0 0 0O0000 0 3hMUUOU0OU0 0 2 04uMQOO000 20H L Realtime PCR Master Mix UUU UUUU DI DI DI DI cos ge n mn Do of SAT DI LD oo Dn DI DI Ea o H Ses CI Oo D D mn DI Dj DI DI O a m DI CD oo g oao fe Co O a CI DI DR DU D g oaoa DI DI DI Co O a H e Dn SS oO oOo OO g oao Saro a Cha oO DI 290900 nnn O05 n lt ZH Dn Sel n lt ZH D n Hoo CI n 290900 Doo oo spec mi DI Ooo ue cis Pe Eel n DA Do DI DI DI Fi O n Cc DI D
5. CI mn n r Z D DI D oo ogan DI n r Z Dn DI Dn pg DO O00 DI Dogon n DO TE m EL oo Dogon DI lt Da O00 eae 2 HH 00 HD n HD n 9 gga D a Oo 00 HD n n D n soe 2O n o0 DD DD 5O O n o0 DD DD n i i gg Goo mn DI oo ogion n 1 n Goo Bono oft n d d q un BE DED O n n c Goo nm Hi 1 oo mn Lis n D ae DI n 1l amp r Goo m i 1 oo DI nDCnnrngz nnn nm i 1 joo mn nu il NI ll l i DEE DI DLT Ed ID ET A ZI ETI We nu i ll i EZ n j Hl Jl1 1 l 1 H o i e EI ER Dj ETO Oo noooo 420 mn n i Hl ll j Il I j o KERDA a Bar xaw ura xw GER xar asi nm n l i oe moe A D 1 H oo n ll l a l 1l ll D DH DH U lj Sipe Hl H a boo i Hl 1 HI H ff n ll j I j 1 nm j 1 Hl j Hl j Hl ao Seon Sie eee nm l 2 El ll 1 DD 2A SS Sl ey oao a DI Hm E SL GI Ez En 72 0 x40U00D0 data collection PCR0 0 00 0 RO000000000 60 0 0 95 C 600 1 95 C 150 60 C 150 72 C 300 Q00000000010000 RT PCR IE n DH mn mn H cDNAG 0 0 DNAD DNAD DI D00000000000RNAO OO PCROOQOO HOUUU 10000 RT PCRO DOD UUUU UUUU W
6. CR UUU BI PRISM 77000 0000000000000 UUUUUUDO B H li OO00 LineGeneg UUDUDUUDOUUUDO UUUUU UUUUUD UUUUU0UU0UDNA 000PCRO0000000000000000000 cDNA dNTP MgClz Taq DNAD IJL LIHHH HHH DD Di O 1st strand cDNAL I i B R NE R N E EH E B N OOO DNAD D 000 DNALI O Li li 00 l1 li OT OOOOODODNA OU UUUU O g OOO PCERE Li Li l L j jJ J 10 10ng 14 uL 25 ul 2 NL 2 HL 2 NL 5 UL 50 uL PCROOOM MOOO00 044 MI TOO 024 MI DODO 50y L 0 O ABI PRISM 77000 0 0 O TaqMan g 0 0 0 000000 Realtime PCR Master Mix B RE EE B H ENE UUU UUUU ff n l ll i Il Il Tl ee en eee En Hm o Wo Oo Oo mn EDE NEL YEK a Do Soo Fo Oo co o ao Do Goo Fo Oo HD oF DO En Ooo g On 80 o ca BC of5o En Ho 60 o ao o gt opi Oo D g o d D DH Co o H DH Hi o g o Di m DH Co o H DH c H Bo HD ogoogo o H O50000 Co HD mn H O20000 Dn oo ogomo mn H O50000 Og Da n DD oH Ossona Oo 250 2n DD DE DD DD Dog 500 n DD oF oFoo0es Og 20 DD mn H 050004 Og 20 mo Coop Og 20 mo Coop DI Bi oo agpo o A ogoo2g H HD oon DED D 9 gD DI Le sie Css r D 2025 mn p9 U27 n D j og O E oo Ga
7. I o H o He n n o0 g HH mh n J 2 HK uu o0 g Ooo eee gog ooo E ll 5 El J D n n ooo F 1 J eo n 2 ooo n n c J 1 n Z n n nh DZ Dog2909 g00 p20 Hm U Oo cnn RT c nn fal es nooo Ooo Oooo Oo DI DI n E oao DZ HH n l g H giano zo nooo oot II j 1 1 D nooo n l H 20 35H Sooo se ee j DI DI a n coo o ao n D i i Too n U OOo i a E ELE r22 E D i n 1 i Too ele HH D D D DH 1 1 Il j 1 Ooo oand d DH i ll ll 1 j se od oo nooo Oooo ET mi nm Em nm Dl ooog x HH Hi 1l Hl j HI coeso Soe n ll j 1 1l DD H o o No o mn 75 C 300 x 40 000 data collection PCROOOO MIO000 50 C 150000 95 C 300 1 95 C 50 50 C 150 75 C 300 1 8 uL 5 uL 0 4 uL 0 4 uL 0 4 uL 10 uL PCRO 0 0 0 00000 0 4k MQO000 0 2u MI ji l1 1 10u L Realtime PCR Master Mix 0001200000000 0 BioFlux LineGene 0 0 0 TaqMans DOO A000000 Realtime PCR Master Mix UUU UUUU oO o 00 HS Gea 2 co o eo Di Sao D HH D Je DH EH oF Feo 2 So 20 D El rat n ce i GA DD opo 2 CI CO Co nm n H O z H CI DP Co CM Co oo DH n Hi pe ogo20 ie Desa DI DI DO ofn0o0 DI O ggo DI DI DO dg 000 O ggo i o gE 00 25 Sei DO DH bp Lil D DI an j E REW rex D DI
8. Realtime PCR Master MIX Code No QPK 101 QPK 101T HOUUU TOYOBO CO LTD Life Science Department OSAKA JAPAN Ho ag 000000000000000000000000000a 42 0000000000000000000000000000 2 0000000000000000000000000000 3 0000000000000000000000000000 3 0000000000000000000000000a0a 4 0 O0 ABI PRISM 77000 000 TagManT g GO 000000 4 0 O Roche LightCycier g Ii 0000000000000 0000000 00000000000000g0o 9 OO BioFlux LineGeneg DO 0 TagMan g i 000000 6 00000000000010000 RT PCROO00000 7 000000000000000000000000000a 9 0000000000000000000000000000 10 Hood Og H H E A1 Ll VER B U NEN E H li NLN L SE EI BR EN UA TEO TEA HI H SJ H li D0000000000000000000000000000000 H B E HER ERN KEN EU aNER EN ji F Hoffmann La Roche Ltd Roche Molecular Systems Inc The Perkin Elmer Corporation Purchase of this product is accompanied by a limited license to use it in the Polymerase Chain Reaction PCR process for The Research Field in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up front license fee either by payment to Perkin Elmer or as purchased i e an authorized thermal cycler TM
9. SYBR Green Realtime PCR Master Mix 1mLx 1 1mLx SO QPK 201T QPK 201 TI j HH 1 li ji DO0OD000000 00000000000000 HOUUUUUUUUUU DUO 530 8230 GO 0000000002080 TEL 06 6348 3786 FAX 06 6348 3833 E mail order_lifescience bio toyobo co jp HOUUUUUUUUUU DUO 0 103 8530 GO 0000000000 170 90 TEL 03 3660 4819 FAX 03 3660 4951 E mail order_lifescience bio toyobo co jp 00000000000000000 O00O0000O TEL 06 6348 3888 FAX 06 6348 3833 0000 9 00 12 00 13 00 17 00 p 00 00 00 00 E mail techosk bio toyobo co jp DURLD http Awww toyobo co jp seihin xr lifescience html
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