IO テスト PE 標識抗体 HLA
Contents
1. 7 8 2 8 C 12 IO PE HLA DR IOT2a IM0464U 100 2mL2. 1 20 wL 1 PBS PBS 6603369 1 500mL pH 7 2 0 2 BSA 0 5 0 1 2 PBS VersaLyse PBS 1mL IOTest 3 A07800 12 5L 0 19 PBS VersaLyse PBS 1mL IOTest 3 A07800 67 5 L 0 59 PBS 3
3. 5 6 7 Ficoll Paque 8 9 No reo
4. A09777 VersaLyse IM1400 OptiLyse B A11895 OptiLyse C A07799 IOTest 3 4 IOTest Mouse IgG2a PE A09142 100 2mL 5 Ficoll Paque Phamacia 17 0840 03 2 1 100 L 5x103 mm3 10 x 103 mm PBS 3x103 mm 1 25C 500 x g 5 2
5. EPICS XL vs PE 4 5 Leukocytes Lymphocytes 68 Count 28 48 SS 299 389 688 199 1989 1 1 18 PE 189 1988 1 1 18 PE Fc lOTest CD45 FITC CD14 PE A07738 IOTest T
6. 3 4 DxH800 5 PBS 5 10 x 103 mm3 1 12mm x 75mm 2 100L 3 20L IOTest Mouse lgG2a PE 20L 4 15 5 6 PBS 2mL 7 400 x9g 3
7. 24 FS 90 SS PE Log 29 29
8. T B8 12 2 lIgG2b HLA A6 CTL PE Phycoerythrin 486 575nm 560 590nm HLA DR HLA DR PE 1 IO 2 EDTA 3 6 4
9. o CD8 HPB ALL 1 HPB MLT 2 90 CD8 CCRF CEM 3 19 1 HPB ALL 2 HPB MLT 3 CCRF CEM PBS 4 CD8 HPB ALL HPB MLT 3 10 3 5 5 1 0 196
10. IM0464U Seconn COULTER I B IO PE HLA DR IOT2a 1 2 3 4 IO PE HLA DR IOT2a PE IgG 2012 10 1 20200EZY00111000 HLA DR 29 34kD B8 12 2 MHC TI HLA DR T B T HLA DR B
11. 8 6 7 9 0 5 1mL 0 19 0 59 PBS 10 24 3 Ficoll Paque 1 3 4mL PBS 2 Ficoll Paque 4mL 1 3 2 8 400 x g 30 4 Ficoll Paque PBS 2 8 C 400 x g 8 PBS 2 8C 400 x g 4
12. 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC CO IO O IM0464U_1210
13. PBS 2 lt 8 400 xg 3 PBS 5x10 mm 5 x 10 mL 11 90 1 12mm x 75mm 2 Ficoll Paque 5 x 10 5 x10 mL 100 L 3 20L IOTest lgG2a PE 20 L 2 8C 30 PBS 2mL 2 8 C 400 xg 5 6 5 7 0 5 1mL 0 59 PBS 8
14. B NK 1O
15. 1 Jenning C D Foon K A Recent advances in flow cytometry Application to the diagnosis of hematologic malignancy 1997 Blood 8 90 2863 2892 2 Krensky A M The HLA system antigen processing and presentation 1997 Kidney International suppl 58 51 2 7 3 Lee J Dupont B O The HLA system An introduction 1990 The HLA system A new approach Springer Verlad 1 26 4 Wade W F Davoust J Salamero J Andre P Watts T H Cambier J C Structural compartmentalization of MHC class signaling function 1993 Immunol Today 11 14 539 546 5 Szollosi J Horejsi V Bene L Angelisova P Damjanovich S Supramolecular complexes of MHC class MHC class Il CD20 and tetraspan molecules CD53 CD81 and CD82 at the surface of a B cell line JY 1996 J Immunol 157 2939 2946 6 Uckun F M Regulation of human B cell ontogeny 1990 Blood 10 76 1908 1923 7 Kontny E Ryzewska A Surface markers on human activated T lymphocytes IV Comparison of high affinity E rosette receptor expression with the expression of other activation markers receptor for Interleukin 2 MHC class antigens 1990 Archivum Immunologiae et Ther Experimentalis 38 421 431 8 Huang S Terstappen L W M M Lymphoid and myeloid differentiation of single human CD34 HLA DR CD38 hematopoietic stem cells 1994 Blood 6 83 1515 1526
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