Profinia - Bio-Rad
Contents
1. PB Ready to Use 22. SC Bic Profinia Native IMAC A Toe ALE 0 30 min Native IMAC 2 30 2 tae VE Pa Veg Wey eN a Hier baske dd OC mare mig so ao eae Profinia PC AS UI a a eee Ce EDL AFA 0 5 4 hr
3. USB Run Data Life Science Group Veolia oe a el a at i ge 0 6 0 Ej CEE We ie 8 te A Step by Step 5 ds 10 Seg I EO 7 Ole a keeps A CE CEE Te Run Elapsed Time
4. lt RE 2R 05 D7 Va 5 91a p esd Om AS lt a A Lies TY icles Self Cleaning Self Cleaning 688O020 VCS Bi hae ee ale Ar es ee HE SUG aaa Sica Coins 2 Gl se BSI es Profinia USB ERT aes PCCIEER SS AC 2D eEs CS 8 Poonma CR ss al ND Profinia 5 Yi so eigen Bega
5. Run Run Poma 2 wr Mame a Ce Ce ees 2 eo ela AIERT Sie Olea fae Gig Prehinia A FALO Pee Profinia METHOD 1 ml Native IMAC Desalting Mi SELECT METHOD TYPE amp OPTIONS METHOD TYPE SAMPLE Native IMAC O 1Sample Native IMAC Desalting 2 Samples O Denaturing IMAC O GST Desalting O 1ml 10 mi Desalting O 5 mi 50 ml O Next Method selection screen METHOD 1 ml Native IMAC Desalting Mi RUN IN PROGRESS Elapsed Total Run Time 00 02 7 01 07 HRS Current Step Equilibrate C2CDS Step Time Remaining 238 SEC Sample 1 Expected to Elute at 11 48 AM REAL TIME DATA AT gt 11 14 10 AM Flow Rate 4 00 mlsmin C Collection W UV Cartridge 2 0 31 AU
6. 19 000 620 0225 Profinia Native IMAC 47 000 me NY IPH 1ml Profinia Native IMAC 10 1 1ml IMAC Profinia 2 10ml 2 620 0229 Profinia Native IMAC 50 000 620 0235 Profinia Native IMAC 90 000 Profinia Native IMAC 5ml Profinia Native IMAC IMAC 1ml 1 2 5mlIMAC 10ml 1 148 50m 1 E coli 1 620 0227 Profinia Denaturing IMAC 39 000 620 0230 Profinia GST 58 000 1ml Profinia Denaturing IMAC Profinia GST 1 1mlIMAC 1ml GST 2 2 10ml 2 E coli 620 0237 Profinia Denaturing IMAC 80 000 1 glutathione 1 5ml Profini
7. Bio Gel P 6 Bio Scale Mini DY 1 His tad GST tag ee 6 2 His tag GST Erofimla His tag GST See Om Uy ea Ye ae eee NCO eo joc Ome Ss eI Poa 7 e OA Cia 02Mm lt a b HEA 2 Profinia Protiniatg 2 4 A Plata J Y OW rss Oils Bio Scale Minia UY Ty vr 7 o 7 5 iS x S S a GST at eee ea Sa SU Profinia
8. 12 16 hr 920 hr es sexe Bp hr A Bis L F W1W2E EE SS _ Er TI MEE mg mg ml J20kKD Ecoli2 7 7 7 8 2 6 94 20kD E coli His tag A Profinia Denaturing IMAC Denaturing IMAC 30 Lf B SDS PAGE Plus 0 Was 1 W1 Wash 2 W2 Elute E EMAA ZAINI Proteins Purified Under Typical Run Conditions Profinia ra ol ME Pona lt IO EI ee ee F His tag GST ppl3029 a nD a ees 5 UV fe 2 Beg J UY eee S NAC Dive 7 Ss yee Fee EMG
9. ales A Sao ao FP elaBio Sedle Minis i NY Fe Oe ae 3 s lt T Profinia Bio Scale Mini IMAC IMAC e e e e IMAC e e e IMAC e e e e IMAC e e IMAC e e GST GSAS os e e e e e GST e e e e GST e e e A Re oO 7s ZEVEIMAC Y kicik AED k U y VE lca EMIS SEN CORA Life Science Group K iN lv P D Profinia
10. 140 0002 2 2 24 TEL 03 6361 7000 FAX 03 5463 8480 47532 0025 1 14 11 TEL 06 6308 6568 FAX06 6308 3064 7812 0013 2 18 30 TEL092 475 4856 FAX 092 475 4858 TEL 03 6404 0331 FAX 03 6404 0334 C2123 0705A
11. Conductivity 0 03 mS Method selection screen Run Native IMAC desalting His tag Run His tag UV 138 7A Ft on Profinia His tag GS 3 IMAC Profinity GST Profinity
12. Affinity Purification Profinia Protein Purification system Purify your samples Simplify your life 4 4 BBY ATL Purify your sample Simplify your life Prolinia Profinia ie
13. Profinia GST 3 4 95 MIr ER 8 Tee 0 2 20ml min 45psi 3 4bar A 30 C 2 999ml T USB USB PC UV 280nm FOC Windows 2000 XP 0 500ms cm pH pH 1 14 57X36X69cm WXDXH 8 4 X2 15ml 50ml 57X38X77cm WXDXH 24kg Ordering Information Profinia 620 0238 Profinia 55 000 620 1005JA Profinia Native IMAC 2 400 000 50ml Profinia Pro
14. a Denaturing IMAC Profinia 2 5ml IMAC 620 0220 Profinia Bacterial Lysis Extraction 1 35 000 1 620 0203 Profinia His 1 18 000 620 0226 Profinia GST 55 000 620 0204 Profinia GST 1 18 000 1ml Profinia GST 620 0233 Profinia 1 12 000 WW ae 1 1ml GST 620 0200 Profinia Urea 45g 2 3 000 2 10m 2 620 0201 Profinia Urea 90g 2 5 000 620 0236 Profinia GST 100 000 620 0202 Profinia Glutathione 1 23g 1 2 500 5ml Profinia GST 2 5ml GST 1 50ml 1 620 0228 Profinia 28 000 10ml Profinia Arts 1 10ml 2 BIO RAD http discoverbio rad co jp
15. eve Gees 2 Elesige wie 7 A dan 2 oo UWS AO DE pe ea ae ae PUY IS 3 ew endo OS ae a eE uel YD ve Ss AR Gia eS Vem ie ZI iS UP eee re de mg His tag 1ml 75kD Escherichia coli High Profinia Native IMAC 15 98 37kD Bacilus subtilis Putative asparaginyl hydroxylase High Profinia Denaturing IMAC 5 0 90 yxbC product 32kD Saccharomyces cerevisae NGG1p interacting factor nif3 High Profinia Native IMAC 6 0 90 28kD Aequorea victoria Green Fluorescent Protein GFP High Profinia Denaturing IMAC 10 1 95 17kD Agrobacterium tumefaciens High Profinia Native IMAC 55 98 10kD Saccharomyces cerevisae RNA processing protein Sme1p Medium Profinia Native IMAC 1 4 95 GST ml 101kD Escherichia coli High Profinia GST 10 0 88 76kD Escherichia coli High Profinia GST 37 82 44kD Homo sapiens Endiribonuclease inhibits translation Low Profinia GST 55 82 26kD Schistosoma japonicum Glutathione S transferase GST Medium
16. finia Profinia 2 50ml Profinia Native IMAC F PU HE 620 1006JA Profinia GST 2 400 000 Profinia Profinia Profinia 620 0221 Profinia Native IMAC 30 000 Profinia GoOT Profinia 10 620 0010 Profinia amp USB 150 000 620 0222 Profinia Denaturing IMAC 35 000 620 0410 Profinia 75 000 1 4 Urea 10 50ml 2 620 0223 Profinia GST 30 000 15ml 2 1 2L 2 glutathione 10 Profinia 620 0224 Profinia
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