Blend Taq
Contents
1. 1 2 2 3 MTA Material Transfer Agreement http www asterand com2. Lane M 1kD DNA Blend Taq Lane 1 RI Ja Lane 2 DNA 5ng Taq NA PCR Taq went NRN Ne PCR TA MO ES Blend Taq Taq 3 4 4 High Fidelity KOD KOD Plus KOD Plus Ver 2 Blend Taq PCR http www toyobo CO jD bio Barn
3. PCH KOD Dash 50UX 1 o0C LDP 101 o5 000 TArget Clone 10 MagExtractor PCR amp Gel Clean up 00 Mag ical Trapper 1 TAK 101 12 000 NPK 60 1 5 000 MGS 101 38 000 AL Asierand 40 10 2004 HE RNA FFPE RNA DNA
4. PCR Blend Taq Blend Taq Plus 23 0kbD 1 Blend Taq Blend TaqW Plus ge M 1 3 4 5 6 7 8 M Blend Taq Blend Taq Plus A Lane M 1kD DNA Lane 1 8B globin T 3kb Lane 5 Myosin h c 6 2kb Lane 2 8B globin kD Lane 6 gloDin 8 5kb a _ Lane 3 B globin 3 6kb Lane 7 8 globin 17 5kb 1 Lane 4 Myosin h c 5 2kb Lane 8 globin 23 0kb DNA _ BlendTad BlendTaqY Pluse A Human 8 globin 3 6kb II Blend TaqW Blend Taq Plus DNA 5ng
5. KOD Dash PCR Blend Taq KOD Dash PCR PCR PCR Blend Tag KOD Dash Taq DNA 3 5 Exonuclease DNA DNA 3 5 Exonuclease KOD _ DNA KOD DNA DNA KDD DNA
6. 10xBuffer for Blend Taq 2mM dNTPs 1 21 9 Forward primer 10pmole gl Reverse primer 10pmole gl Blend Tage Total KOD Dash D W 1 23 10 xKOD Dash buffer 3 2mM dNTPs 3 Forward primer 10pmole gl 0 35 Reverse primer 10pmole 0 35 KOD Dashe 0 3 Total 30 ul Blend Taq 55C 4 5min KOD DashS 60C 8 5min x 30 cycles 94C 94 C A C 1min 1kb Tm 5 10 C x 30cycles 94C 94 C 72 C SeC B sec A C 30sec 1 kb Tm Tm 5 4min 30sec 30 1 1 PCR 15 PCR MagExtractor PCR amp Gel Clean up kit Blend Taq KOD Dash M1234M12 3 4 lt 4 5kb Lane M 1kD Lane 1 4 3 PCH 0 4 3
7. Template DNA 5 200ng 4 Blend Tag 2 5units ul 0 5 Total 5Ogl PCR G6kb 3 6kb 2 10kDb dNTP 0 3mM Target Number Segment Of 1 kb 6kb Cycles 94C min Denaturation oC Primer Tm 5C 55 60C 3 Annealing 30 30sec 58 72C 72C Tmin kb PCR target 4 Extension 1 min 1mn kb PCR target 1 98C 5 10 6kD Tm 70C 1 Human globin human myosin heavy chain DNA100ng 00ng
8. 4 ek 1 ek4 CodeNo Blend Tad 50Units X BTQ 101T 5 000 50Units X 1 BTQ 101 19 000 3 en O a 1 000Units x 1 BTQ 102 6a2000 1 000Units x 3 BTQ 103 170 000 Blend Taq Plus 50Units x 1 BTQ 201T 6 000 Hot Start Version 50Units X 1 BTQ 201 1 000 Taqn PCH glBlend Taaq PCR PCR Pol I Taq Pol I DNA PCH Pol1I 3 5 Exonuclease Proofreading KOD Pfu o 5 Ex
9. Taq DNA 19 000 550U Blend Taq Plus 30sec 1kb PCHRH PCR 1 TA TArget CloneW pTA Vector 4 5kb PCH PCR JM 1098 Blend TaqW KOD Dash 5 196 PCR 1 Primer F primer TCGAGGTCGACGGTATCGAT 20mer GC 5596 R primer CGCTCTAGAACTAGTGGATC 20mer GC 5096 Blend Tag D W
10. o Exo PCR Pol I Pol I C Blend Taq Blend Taq Plus Taq DNA Blend Taqg Plus Taq PCR 13 PCR mixture D W de io WI 10XBuffer for Blend Taq BS mM dNTPs 5 Primer F 10uM Primer R 10 M
11. s W M Proc Natl Acad Sci USA 91 2216 2220 1994 Blend Tag PC1 RT PCR PCR M 1 2 3 4 5 6 7 8 1 000bp 500bp Crassostres gigas A 1 4 Crassostres gigas B 5 6 TO0bp Blend Tag 0 050U 10 gl M 1 3 Lane M 100Dp Lane 1 Blend Taq Lane 2 Blend Tag Plus Lane 3 A ES RT PCR M M S 6 A 0 125U 10l 7 A 0 050U 10 l 8 6 3 A 0 025U 1 0 1 3 Lane 1 Blend TaqW Plus Lane 2 Blend Tag Lane 3 A F4 ek 1 ek4 4Blend Taq
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