MY4-RD1
Contents
1.2. CD14 T B CD2 CD3 CD5 CD19 CD20 MY4 MY4 CD14 GPI GPI PNH MY4 Ficol paque 7 Fc y
3. 1 2 3 2 8 20 25 1 MY4 RD1 6603262 100 Bernard A Boumsell L Dausset J Milstein C Schlossman SF Leukocyte Typing 1984 Berlin Springer Verlag 108 407 McMichael AJ Leukocyte Typing nl White Cell Differentiation Antigens 1 987 Oxford Oxford University Press 586 589 Griffin JD Ritz J Nadler LM Schlossman SF 1981 Expression of myeloid differentiation antigens on normal and malignant myeloid cells J Clin Invest 68 932 941 Nadler LM Rit
4. FAB TCR lgH T non T MY4 RD1 CD14 CD14 55kDa LPS LPB MY4 RD1 CD14 FAB
5. 30 2 10 15 20 30 2 PBS 8 0g L 2 12 2 9gL 1 0 2g L 0 29 L pH7 2 7 4 BSA 0 19 0 1 3 MslgG2b RD1 6603038 100 4 Propidium lodide Calbiochem 53705 0 01mg mL 0 05mg mL 5 Acridine orange Baker A366 3 0 005mg mL 6 Ficoll paque Pharmacia 17 0840 03
6. 3 4 LH 700 5 10x10 L 1 100L Ficol paque 1 4mL PBS 2 Ficolpaque 4mL 1 3 400 xg 30 4 Ficoll paque 5 PBS 4C 400 xg 8 6 PBS 4 C 400 xg 4 7 PBS 4C 400 xg 3 8 PBS
7. 5 Ficoll paque
8. 859 CD14 Mo2 322A 1 MY4 BALB cJ P3 NS1 1 AG4 1 lg lgG2b H z L MY4 RD1 Phycoerythrin PE PE Protein 0 5 1 5 468 lt 575nm 568 590nm 1 0 5mL 0 2 0 01M 0 15M 0 196 CD14 488nm
9. 11 25 000 1 x 10 L 25L 12 Propidium bdide 0 01mg mL 10L 13 30 Acridine orange 0 005mg mL 10L 3 14 15 100 859 6 1 1 12mm x 75mm 2 100wL 3
10. 2 Ficoll paque 1 12mm x 75mm 2 Ficolpaque 1 x10 1x10 L 1mL 3 4C 400 xg 4 4 200L IgG MslgG2Db RD1 5 4 C 30 6 PBS mL 4 C 400 x g 4 7 6 2 8 PBS EPICS Cytomics 3 Ficoll paque
11. CD14 x100 Fc 1 29
12. 7 BSA NBS PBS CD14 10 000 L PBS 10 000 MY4 RD1 gt 1 5L 1
13. 6603262 2009 11 2007 2 20900AMY00143000 877429 MY4 RD1 IN ww 1 CD14 55KD CD14
14. 1 1 x 10 200L Ficoll paque 200L U 96 2 4 C 400xg 5 3 4 5 200L IgG MslgG2b RD1 6 4 C 30 7 4 C 400 xg 5 8 3 4 9 PBS200L 4 C 400 xg 5 3 4 PBS20
15. 200uL PBS BSA 0 19 NBS29 0 19 BSA NBS 1 1 2 1 6603152 300 PBS 24mL 1mL 2 1L 8 26g 1 0g EDTA4 2 37mg pH7 2 lt 7 4 1 1 2
16. IgG MslgG2b RD1 4 45 5 a b a 2mL 5 15 b PBS 400 xg 5 1mL 30 2 250L 6 PBS 7 4 C 400 xg 5 8 9 PBS 10 EPICSCytomics
17. MY4 RD1 CD14 MY4 RD1 y 1 08X 1 50 r 0 93 n 56 1
18. 1 EDTA 3 10 x 10 L 10 x103 L 3x10 L PBS 20 25 6 2 4 NO a gt 10 x 10 L 10 20 x10 2 20 30 x10 23 30 40 x10 5 40 60 x10 6 60 100 x10 10 100 200 x10 20 37 PBS b lt 3 x 10 L 1 25C 500 xg 1 700rpm 5 2
19. RD1 FS 90 SS 1 4 1 69 20 EDTA 6 90 85 Ficoll paque Ficoll paque
20. 1 x10 wL 1 x 10 mL 9 Fc 10 15 1mg mL 10 a b 90 a 11 1 x 10 1x10 L 1mL 12 PBS 4 C 400 x g 4 13 0 05mglmL Propidium lodide 3 1 14 PBS 4C 400 xg 4 15 14 16 859 b
21. AML M4 M5 56 1009 MY4 AML M1 M2 33 MY4 AML M3 M6 ALL MY4 AML CD14 95 5 MY4 CD14
22. 0L EPICSCytomics 10 3 4 1 1 2 29 NN 1 MY4 RD1 Ficoll paque 200L PBS 109 20L 2 1 3
23. 2 271 5 2721 Kielian TL Ross CR McVey DS Chapes SK Blecha F 1 995 Lipopolysaccharide modulation of a CDI 4 iike molecule on porcine alveolar macrophages J Leukoc Biol 57 581 586 Qing G Rajaraman K Bortolussi R 1995 Diminished priming of neonatal polymorphonuclear leukocytes by lipopolysaccharide is associated with reduced CD14 expression Infect Immun 63 248 252 Yasui K Komoyama A Molski TF Sha afi RI 1994 Pentoxifylline and CD14 antibody additively inhibit priming of poiymorphonuclear leukocytes for enhanced release of superoxide by Iipopolysaccharide possible mechanism of these actions Infect Immun 62 922 927 Yang Z Khemlani LS Dean DF Carter CD Slauson DO Bochsler PN 8 1994 Serum components enhance bacterial lipopolysaccharide induced tissue factor expression and tumor necrosis factor alpha secretion by bovine alveolar macrophages in vitro J Leukoc Biol 55 483 488 Shapira L Takashiba S Amar S Van Dyke TE 1994 Porphyromonas gingivalis lipopolysaccharide stimulation of human monocytes dependence on serum and CD14 receptor Oral Microbiol Immunol 9 1 12 1 17 Parker TS Levine DM Chang JC Laxer J C offin CC Rubin AL 1995 Reconstituted high density lipoprotein neutralizes gram negative bacterial lipopolysaccharides in human whole blood Infect Immun 63 253 258 Pedron T Girard R Turco SJ Chaby R 1994 Phosphatidy linositol anchored molecuies and inducible lipopolysacchaide b
24. e myeloid leukemias Leukemia Res 10 279 290 Griffin JD Davis R Nelson DA Davey FR Mayer RJ Schiffer C MclIntyre OR Bloomfield CD i986 Use of surface marker analysis to predict outcome of adult acute myeloblastic leukemia Biood 68 1 232 1 241 Medeiros LJ Herrington RD Gonzalez CL Jaffe ES Cossman J 1991 My4 antibody staining of non Hodgkin s lymphomas Am J Clin Pathol 95 363 368 4 4 20 21 22 23 24 25 26 27 28 Warzynski MJ Otto RN Steingart RH White DF Rosen MH Hetzel PC Flatow FA Podgurski AE Johnson ML 1991 MY4 expression on B lymphocyte malignancies may be associated with a more adverse prognosis Leuk Res 15 357 365 15 Tasies D Montserrat E Reverter JC Villamor N Rovira M Rozman C 1995 Myelomonocytic antigens in B cell chronic Ilymphocytic leukemia Leuk Res 1 9 841 848 Ziegler Heitbrock HW Pechumer H Petersmann Durieux JJ Vita N Labeta MO Strbel M 1994 CD14 is expressed and functional in human B cells Eur J Immunol 24 1 937 1 940 Pedron T Girard R Chaby R 1995 Variation of LPS binding capacity epitope expression and shedding of membrane bound CD14 during differentiation of human monocytes Immunol 155 i460 1471 Heumann D Barras C Severin A Glauser MP Tomasz A i994 Gram positive cell walls stimulate synthesis of tumor necrosis fector alpha and interleukin 6 by human monocytes Iniect Immun 6
25. indin sites of human and mouse bone marrow cells J Biol ChemL69 2426 2432 Kawakami Z Ninomiya H Tomiyama J Abe i990 Deficiency of glycosyLphosphatidylinocitol anchored proteins on paroxysmal nocturnal haemoglobinuria PNH neutrophils and monocytes heterogeneous deficiency of decay accelerating factor DAF and CD16 on PNH neutrophils Brit J Haematol 74 508 513 Ziegler Heitbrock HW Appl B K lt ferlein E Lbfler T 1994 The antibody MY4 recognizes CD14 on porcine monocytes and macrophages Scand J Immunol 40 509 51 4 Ziegler Heitbrock HW Schraut W Wendelgass P Str6el M 1994 Distinct patterns of differentiation induced in the monocytic cell line Mono Mac 6 J Leukoc Biol 55 73 80 135 0063 5 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC oo COULTER 6603262_0911
26. z J Griffin JD Todd RF Reinherz EL Schlossmsn SF 1981 Diagnosis and treatment of human leukemia and lymphomas utilizing monoclonal antibodies In Progress Hematology Vn E B Brown New York Grune and Stratton 1 87 225 Grifin JD Mayer RJ Weinstein HJ Rosenthal DS Coral FS Beveridge RP Schlossman SF 1983 Surface marker analysis of acute myeloblastic leukemia Ildentification Of differentiation associated phenotypes Blood 62 557 563 Merle Beral H Cong Duc LN Leblond V Boucheix C Michel A Chastang C Debre P 1989 Diagnostic and prognostic significance of myelomonocytic cell surface antigens in acute myeloid leukemia Brit J Haematol 73 323 330 Foon KA Gale RP Todd RF 1986 Recent advances in the immunologic classification of leukemia Sem Hematol 23 257 283 8 Foon KA Todd RF 1986 Immunologic classification of leukemia and lymphoma Blood 68 1 31 Hanson CA GajLPeczalska KJ Parkin JL Brunning RD 1987 Immunophenotyping of acute myeloid leukemia using monoclonal antibodies and the alkaline phosphatase antialkaline phosphatase technique Blood 70 83 89 Drexler HG Menon M Gaedicke G Minowada J 1986 Reactivity patterns of monoclonal antipod lt lt s positive on myelomonocytic leukemia cells as defined by esterase isoenzyme analysis Cancer Immunol Immunother 21 188 1 92 Drexler HG Minowada J 1986 The use of monoclonal antibodies for the identification and classificatton of acut
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