Insert Check
Contents
1. 1 1 Insert Check Ready Solution PCR 50ul 2 lt 3 4 1 PCR 5 6 PCR PCR gt 94C 4min 94 C 30sec 60 C 5sec 30cycles 72 C Xsec 7 PCR 82. PCR Colony Direct PCR DNA Polymerase Buffer dNTPs Insert Check Ready Colony Direct PCR PCR PCR PCR XZAP T Insert Check Ready Primer dNTPs DNA Polymerase PCR 1x PCR Primer M13 Primer pBluescript pUC pGEM XZAP IL DN
3. 50 25 100 PCR 1mm 37 6 8 20sec 2kb 30sec 1min 2 5kb Co Cn oo
4. PCR 6kb 5 x 10 2 1 PCR 30 2 PCR 25 PCR 9 1 Takagi M Nishioka M Kakihara H Kitabayashi M Inoue H Kawakami B Oka M and Imanaka T 1997 App Environ Microbiol 63 4504 4510 2 Barns W M 1994 Proc Natl Acad Sci USA 91 2216 2220 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order_lifescience toyobo jp
5. 141 8633 10 2 TEL 03 6422 4819 FAX 03 6422 4951 E mail order_lifescience toyobo jp TEL 06 6348 3888 FAX 06 6348 3833 9 00 lt 12 00 13 00 17 00 E mail tech_osaka toyobo jp URL http www toyobo co jp bio
6. SM Buffer 1 200 SM Buffer 20 20sec 2 2 Insert Check Ready Solution PCR 50ul 3 1 1pl 2 PCR 4 5 PCR PCR gt 94 C 4min 94 C 30sec 30cycles 68C Xsec 6 PCR SM Buffer 1 NaCI 5 8g MgSO4 7HzO 2 0g 1M Tris HCI pH 7 5 50ml 2 Gelatin Solution 5ml 2 SM Buffer Mg Insert Check Ready Solution 50 1 PCR 3 20sec 2kb 30sec
7. 1min 2 5kb 7 PCR 1 15mM K Buffer H Buffer M Buffer 20 PCR L Buffer MagExtractor PCR amp Gel Clean up Code No NPK 601 PCR 2 MagExtractor PCR amp Gel Clean up Code No NPK 601 PCR 8 1 1 1mm 2 GO 98 C 20sec PCR 3 PCR
8. 4 1 UV PCR 2 DNA SM Buffer 5 PCR pBluescript TSK Primer 573 M13 Primer P7 5 CGCCAGGGTTTTCCCAGTCACGAC BssHI 5 TAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGAC 3 ATTGCGGTCCCAAAAGGGTCAGTGCTGCAACATTTTGCTGCCGGTCACTCGCGCGCATTATGCTG Kpm 1 657 Sac I 759 TCACTATAGGGCGAATTGGGTACC M GAGCTCCAGCTTTTGTTCCCTTTAGTGAGGG AGIGATATCCCGCTTAACCCATGG CS CTCGAGGTCGAAAACAAGGGAAATCACTCCC BssHI TTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCAC 3 AATTAACGCGCGAACCGCATTAGTACCAGTATCGACAAAGGACACACTTTAACAATAGGCGAGTG 5 3 CAAAGGACACACTTTAACAATAGGCGA 5 M13 PrimerP8 847 6 Insert Check Ready Solution
9. A Polymerase DNA KOD Dash Taq PCR Deletion Mutant PCR DNA MagExtractor PCR amp Gel Clean up Code No NPK 601 KOD Dash 50 1 _pBluescript Y XZAP Agilent 2 pGEM Promega 2 Insert Check Ready Solution 1mlx 5 Insert Check Ready Solution gt e 0 05U ul KOD Dash s 0 2mM dNTPs e 0 2uM M13 Primer P7 5 CGCCAGGGTTTTCCCAGTCACGAC 3 e 0 2uM M13 Primer P8 5 AGCGGATAACAATTTCACACAGGAAAC 3 1x Reaction Buffer 1 4mM MgCb 3 20 C
10. TOYOBO Ra ldeas amp Chemistry Premix Colony Direct PCR Kit Insert Check Ready Code No PIK 101 TOYOBO CO LTD Life Science Department OSAKA JAPAN A3273K 1 2 nn 3 4 5 PCR 6 7 PCR 8 9 1 DNA
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