Reporter Assay Kit -SEAP
Contents
1. SEAP 4 5 1 1 5 96 22. SEAP AP 37 C 30 96 37 C 15 30 A Reporter Assay Kit SEAP WA 4 X 96 1 5ml 96 Step 1 SEAP 50 500 1 5ml Step 2 4C 15 000rpm 5
3. AP PBS AP AP SEAP AP AP 65C 30 37C HeLa
4. 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order lifescience toyoboJp 141 8633 10 2 TEL 03 6422 4819 FAX 03 6422 4951 E mail order lifescience toyoboJp TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail tech_osaka toyoboJp URL http www toyobo cojp bio
5. Step 3 20 96 Step 4 20 AP Step 5 37C 30 Step 6 1 Step 7 160 1 Lumi PhosSPlus Step 8 37C 15 lt 30 SEAP 15 lt 30 Step 9 5 lt 10 1 5
6. SEAP AP J Berger J Hauber R Hauber R Geiger andB R Cullen 1988 Gene 66 1 10 J Alam and J L Cook 1990 Analytical Biochemistry 188 245 254 B R Cullen and M H Malim 1992 Methods In Enzymology 216 362 368 HH Akhavan Tafti A Schaap Z Arghava
7. 1 2u 1 10 1 20 1 SEAP 5 AP SEAP AP DNA AP SEAP
8. ldeas amp Chemistry Reporter Assay Kit SEAP Code No SAK 101 TOYOBO CO LTD Life Science Department OSAKA JAPAN A3686K 1 2 3 4 5 RRRWNNR WS 1 Reporter Assay Kit SF4P SEAP SEAP
9. ni R DeSilva R Eickholt R Handley B Schoenfelner K Sugioka and Y Sugioka 1994 T Biolumin Chemilumin 9 155 164 1997 CURRENT PROTOCOLS TI 288 298 3 Lumi PhosePlus 100 ml AP 15 ml 0 1 ml Lumi Phos Lumigen Inc HIV HBV AP
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