全ページダウンロードはこちら - 東洋紡ライフサイエンス事業部
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1. A eo 1 1ul 1 3KD 2 1 10 1 3 1ul 4 1 10 1ul M 1kb DNA Ladder KOD FX Neo 6 PCR PCR K OD FX2. PCH KOD DNA polymerase Taq DNA polymerase KOD SYBR qPCR Mix Taq CCCGCCCGAGAGAGTGACTCTCACGAGAGCCGC GAGAGTCAGCTTGGCCAATCCGTGCGGTCGGCG i GCCGCTCCCTTTATAAGCCGACTCGCCCGGCAG CGCACCGGGTTGCGGAGGGTGGGCCTGGGAGG 104 GGTGGTGGCCATTTTTTGTCTAACCCTAACTGAG AAGGGCGTAGGCGCCGTGCTTTTGCTCCCCGCG CGCTGTTTTTCTCGCTGACTT W GC 64 219 bp Homo sapiens telomerase RNA TR gene promoter and complete squence DNA ChIP RNA TR
3. PCHR PCR 15sec kb DNA PCR 30 60sec kb 3 PCR DNA 1 kb 1 10kDb PCR PCR 50I KOD Multi amp Epi KOD Multi amp Epi DNA
4. 1 1 http Ilifescience toyobo jp 2015 9 25 2015 11 30 http lifescience tOyobo cO jD Dage dUuiZ MS 1 10kb Chrome9 1kb MSHG 1 8kb BRCA2 2 3kb WT 1 2 5kb FANCE 3kb RAD51D 4kb KRAS 5kb BRCA1 7kb DDB2 10kb 1 1 9 Multiplex OO ON OO OT p 1 4 LILITs LIALls UPLOAD 5 2 000 ldeas amp Chemistry HPCK 530 8230 8 TEL 06 6348 3786 FAX 06 63848 38383 E mail order
5. 1 KOD SYBR qPCR Mix RNAi mRNA PCRH mRHINA 1 cDNA FU RNAi 35 45 49 mRNA 6 mer cDNA PCR KOD SYBR qPCR Mix H 0 1 6 gl KOD SYBR qPCR Mix 10 ul 50 x ROX 0 4
6. NE Epi genetics p dU AT A 1 in Re Multi amp Epi DNA Metagenomics gt m0 NR 2 kb 1 0kb PCR GC DNA
7. PCR Tkb DNA DNA 50ng 2 5 I Md I M 100bp DNA Ladder 1 KOD Multi amp Epi 2 B Multiplex PC 3 A Taq PCR 4 A Long PCR 1kbp lkb 500pbp 500pbp 1kb DDB2 200 bp FANCG 250 bp HBg 300 bp CDH1 400 bp chrome9 500 bp ERCC4 550 bp 100pbp HRAS 600 bp PRF1 700 bp BRCA1 800 bp 100pbp CDK4 1 000 bp PCRH PCRH 94C 2 min 94C min 98C 10 sec 98C 10 sec 6O0C 1 S30 so 5 cycles 60C 30 seo 5 cycles 68C 15 sec 68C 60 sec 4 hold 4C hold PCR 1 10kb DNA DNA 50neg 2 5 I M 1 2 4 M 1 2 3 4 M 1kb DNA Ladder 10kbm 10kbp 1 KOD Multi amp Epi 5kb 5kbp 2 C Multiplex PCR 3 D PCR 4 A PCR 1 10kb lt gt Chrome9 1 kb MSH6 1 8 kb BRCA2 2 3 kb WT 1 2 5 kb FANCE 3 kb RAD51D 4 kb 1kb
8. ER sf a mp cuide Code No PE 5 siRNA a ec Santa Cruz Biotechnology CRISPR Cas9 1 acte ot cat Te omc ON System CRISPR Cas9 Cas9 93 gRNA 18 000
9. 01JKe7 14 2015 103 PCR DNA PC KOD Multi amp Epi PCR DNA 2015 9 7 28016 3 18 PCR KDD DNA Polymerase DNA s A OO sl 1CiGLIA mRNA LULIHL 4 CE lOUKBOO2 0 PCR Primer PCR PCR PCR FF TOTO CC HcLLILJeLHHcLLr A A FPPPPPPP PF 2 9 1
10. 3B Re 0 103 _Wildtype _ TF2 TF18 TF68_ 1 FU RNAi TF2 TF18 TF68 2 RNAi FUL2 ORF 744Dp 6 FU KOD SYBR qPCR Mix THUNDERBIRD qPCR Mix C AC TF18 35 45 35 45 35 45 49 35 45 49 3545 49 35 45 49 Wild type TF2 TF18 TF68 2 2 0 1 5 1 0 3 0 0 5 A THUNDERBIRD qPCR Mix B KOD SYBR qPCR Mix C RNA 35 45 49 35 45 49 35 45 49 35 45 49 35 45 49 Wild type TF2 TF18 TF68
11. AT DNA Jurkat DNA 900 1 500bp DNA KOD Multi amp Epi 1 5 kb 1 583Dbp TArget Clone Plus KOD A B Multi amp Epi DNA PCH DNA PCH 4 4 4 PR PR PR EpiTect Fast DNA Bisulfite Kit QIAGEN 1kbp
12. DNA gERNA GeCKO v2 CRHISPR Cas9 DNA HDR Homology Directed Hepair Cre Vector eos gANA Dinds t0 target locus n genomic DNA adjacent to he PAM ste Cas9 cleaves the 5 exons of the oene targeted Dy the three ORNA Dias mids at three specitic sites Target gene disrupted URL http www scbt com research crispr cas9 knockout plasmids html MKe7 10 2015 voL103 HOT PCR KOD FX Neo KOD DNA polymerase
13. KOD FX PCR KOD FX PCH 20 30 gt PCH KOD FX Neo KOD FX gt M Takagi et al App Environ Microbiol 63 4504 4510 1997 1 DNA 40kb 30sec kb 1min kb GC
14. PCR DNA KOD Multi amp Epi KODD DNA UKOD PCR KOD Multi amp Epi PCR DNA Taq 1 1 Multi plex PCR NGS
15. RNAi mRNA FU PCR RNA mRNA KOD SYBR qPCR Mix PCR mRNA RNAi siRNA
16. 40 lt gt 3 TDoL 1 Skb Primer 1 ATGTCACCACAAACAGAGACTAAAGC Primer 2 AAGCAGCAGCTAGTTCCGGGCTCCA 3 1 X M 1 2 3 M M 1 2 3 M M 1 2 3 M sr 1 5kbp 1 5kbp 1 5kbp 1 KOD FX 2 KOD FX Neo 3 X PCR M 1kb DNA Ladder PCR 5 196 KOD FX
17. PCH 1 X PCRH X PCR 1 1 1 Buffer A 500l NN Buffer A 500l Buffer A Buffer A Dilution Buffer 100l 100mM Tris HCI pH9 5 100mM Tris HCI pH9 5 3 1M KCI 1M KCI 10mM EDTA 2 10mM EDTA 2 95C 5 95C 5 PR gw Vorexc lt Vortexi
18. NN Taq DNA Polymerase 1 1 KOD FX Neo KOD FX KOD Plus Neo 6 Pfu DNA Polymerase A Long PCR Taq DNA Polymerase Mutation frequency 100 000 PCR KOD FX Neo DNA KOD FX Neo 40kb KOD FX Neo 30sec kb 1min kb A KOD FX Neo KODFX Long PCR M 1 2 3 M 1 2 3 M 1 2 3 KOD FX Neo KOD FX 94C min 94C min 98C 10sec 98C 10sec 74C 30sec kbD 9 cycles 74C 60sec kb ls cycles 98C 10sec gs i 98C 10sec 5 i 72C 30sec kb 72C 60sec kb 98C 10sec Be 98C 10sec 5 70C 30sec kb 70C 60sec kb 98C 10sec 98C 10sec
19. PCR KOD DNA Polymerase kbD THUNDERBIRD SYBR qPCR Mix Taq 104103102101100 1 KOD FX Neo qa 2 KOD FX aa 3 KOD Plus Ver 2 DNA EE 6 TGFZ 1 9 1 8DD 1 a a er 1 PCR ABI StepOnePlus KOD FX KOD Plus 2kb Taq DNA polymerase oOKkb 2 kb
20. qPCR mix 12 5g Primer F 0 5 Primer R AI Template 1 DDW 10 5 5 PCR lt KOD SYBR qPCR Mix gt A dPCRH gt 98C min 94C min 98C 10sec 60C 10sec 68C 30sec x40 94C 30sec 55C 30sec 72C 1min x45 1 60C to 95C 60C to 95C TaKaRa Thermal Cycler Dice Real Time System TP800 qPCR Mix LT 1 1 KOD SYBR qPCR Mix A dPCH qPCR Mix PCR KOD bash News 015 4 1 6 mem 3 TOYOBO LIFE SCIENCE Oo O O Fm URL http Iifescience tOyODO CO jD
21. E coli DNA I KOD Multi amp Epi KOD Plus ver 2 KOD Multi amp Epi K OD DNA PCR Template Ecolil DNA 100ng DNA I 2 8kb Primer F ATGGTICARATHCCICARAAY Primer R RTGIGCYTGRTCCCARTTYTC PCRH 94C min 98C 10 sec 60C 30 sec cycles M 1kb DNA Ladder 68C 45 sec 1 KOD Multi amp ED _ 4C 2 KOD Plus Ver 2 PCR DNA PCR KOD Multi amp Epi 200UX 1 KOD Multi amp Epi M O0U ul 200 0C KME 101 35 000 xPCR Buffer for KOD Multi amp Epi 50 xPCR Buffer for KOD Multi amp Epi W dNTPs dATP dGTP dCTP dTTP Mg
22. DNA TA TArget Clone Plus Code No TAK 20 1 A TA 01JKe7 12 2015 103 P CR TKOD FX PCR DNA PCH PCR PCR KOD FX
23. CpG RNA TR Taq DNA polymerase qPCR KOD SYBR qPCR Mix DNA MKe7 01 103 0 2 O ree gm Tail PCR cDNA ReverTra Ace qPCR RHT KOD SYBR qPCR Mix A PCH a rp aion Phe oe palin PH a dl KRT10 156bp GC 43 1 1
24. RHNA mRNA PU 7 FU FU HRHNAi
25. DNA 1 5kb PCR KOD Multi amp Epi EE Taq 1 1 KDD FX Neo KOD Multi amp Epi KOD FX Neo KOD Plus Neo Pfu DNA Polymerase A Long PCR PCR Taq DNA Polymerase DNA 8 globin 0 0 25 0 50 0 75 0 100 0 125 0 150 0 2 4kb PCR TA IE 6 96 SN gt a ODO A EP MKe7 2015 103 1 a PCR
26. 1 PCR 1 PCR N 4 Bio Technigues 19 394 1995 DNA BufferA Buffer A B C D E F BufferA a oo 100l 2 100 Tp ot sw 100u 901JKe7 2015 103 13 MD 3 PCR PCR grade water 10 ul 94C min XPCR Buffer 25 Ll 98C 10 sec 2mM dNTPs 10 gal 68C 1 min kb 40 cycles 10pmol ul Primer 1 T 5 ul 4C Hold 10pmol ul Primer 2 1 5 ul KOD FX or KOD FX Neo T al gt Tul gibberellin 20 oxidase 1 5kb Total reaction volume 50 gl Primer 1 TGCTTCCATCAACTGCTGCTGT X PCH PCR Primer 8 TCAGCACGGTAATGGTTCTGTGTG
27. 10 1 10 Wi 10 1 NTC mb IReverTra a i Ace qPCR RT Kit Code No FSQ 101 HeLa total RNA cDNA cDNA 10 5 no template control NTC 3 PCH ABI StepOnePlus PCR no template control NTC 4 We pj 3 FC i hh 2 CC Ch Th 1 4 CA 1 Fast Mode 50xROX reference dye ROX KOD SYBR qPCR Mix 1mlX1 40 QKD 201T 9 800 KOD SY
28. PC PCH KOD FX Neo KOD FX A B KOD FX Neo Neo APCH8 POH PCR mm 8 94C min 98C 10sec 68C 1min kb 4C Hold 40 cycles 4 1mm a IP EN PCR KOD FX Neo PCR PCR 50 20mg ml Proteinase K 10 humic acid PCR DNA
29. 2 0mM TA KOD TArget Clone Plus KOD Multi amp Epi DNA TA TArget Clone Plus Code No TAK 20 1 A TA Ke7 4 2015 103 HOT PCR KOD SYBR qPCR Mix GC KOD SYBR qPCR Mix KOD DNA polymerase KOD SYBR S Green 1 PCR 3 5 KOD exo DNA polymerase KOD
30. a 68C 30sec kb YS SgT 60sec kb le0 cyctes M XHind MII Marker Template DNA 200ng 50 gl 68C 7min 68C 7min 52 4C Hold 4C Hold 3 HBg 40kb 01JKe7 2015 103 11 DNA KOD FX Neo KOD FX M 1 2 M 1 lt KOD FX Neo KOD FX Neo 94C min 98C 10sec 8 5kbp EL FE 2 68C 4 5min YCes M 1kb DNA Ladder 4 Hold 21 001mg KOD FX 94C min Template DNA BR Target Human 8 globin 8 5kb 98C 10sec lt gt 30sec kb 60sec kb 68C 8min 4C Hold E PCR PCR j E PCR
31. DNA 55ng 500bp gt PCR 94C min 98C 10 sec 60C 830 sec 40 Cycles 68C 30 sec kb M 100bp DNA Ladder 4C hold 1 TGF8 917 bp AT 69 9 2 BRCA2 1 134 bp AT 63 9 3 APOE 1 487 bp AT 69 1 4 TGF 1 583 bp AT 61 8 AT MKe7 2015 103 3 TGF 917Dbp 5ng DNA KOD Multi amp Epi 1kb DNA M 100 50 25 10 5 1 0 EpiTect Fast DNA Bisulfite Kit QIAGEN 1kbw PCRH Ob 94C 2 min 98C 10 sec 60C 30 Seo 40 cycles 68C 30 sec 100bpp 4C hold M 100bp DNA Ladder
32. mRNA PCR KOD SYBR qPCR Mix PCH Shima Y Fujisawa M Kitagawa M Nakano T Kimbara J Nakamura N Shiina T Sugiyama J Nakamurga T Kasumi T and Ito Y 8014 Tomato FRUITFULL homologs regulate fruit riDening via ethylene DiosynthesiS Biosci Biotechnol Biochem 78 231 237 01JKe7 8 2015 103 USER S NOTE ChIP qPCR ChIP Chromatin Immunoprecipitation KOD SYBR qPCR Mix DNA QIAGEN QIAamp DNA Mini Kit DNA 202bp F 2 0 Tm 70 1 GC 65 R 0 Tm 77 3 GC 709 KOD SYBR qPCR Mix A PCH
33. 1kbp KRAS 5 kb BRCA1 7 kb DDB2 10 kb PCRH PCRH 94C min 94C 2 min 98C 10 sec 98C 10 sec 68C 5 mn 85 cycles 68C 10 min 25 oycles 4C hold 4C hold Ke7 2 2015 103 Earned Nes HE NGS 10kb 10 10kb 5 GC 70 300bp 6 PCR PCR Covaris Truseq nano LT kit illumina Miseq illumina C1 0 M 1 2 3 4 5 6 ERCC4 10kb 5kb ee M 1kb DNA Ladder 1 KOD Multi amp Epi CASP3 2 C Multiplex PCH 3 A Multiplex PCH 4 D PC WRN 1kb aw 5 A Long PCR 6 A PCR BRHAF XPA PCR DDB2 ER
34. BR qPCR Mix 1 67mlX3 x5 1 000 QKD 201X5 147 000 50xROX reference dye 50I KOD SYBR qPCR Mix SYBRHe Molecular Probes Inc PCR cDNA ReverTra Ace qPCR RT Kit FSQ 101 38 000 PCR cDNA FSsQ 201 38 000 ReverTra Ace qPCR RT Master Mix PCR cDNA DNA FSQ 30 1 40 000 ReverTra Ace qPCR RT Master Mix with gDNA Remover 10I 01JKe7 6 2015 103 KOD SYBR qPCR Mix PCR RNAi mRNA RNAi RNA interference RNA
35. CC4 WRN XPA XPC XPC 98C 10 sec 95 velee a a 9 GC 70 1 i 300bp C ho KOD Multi amp Epi Multiplex PCH A Long PCR 3 5 A NGS KOD Multi amp Epi W 10 0 6 1 0 E DNA 100bp C DNA U PCR M C OO S 6 a
36. PCR DNA PCR KUD FX Neo KOD FX Neo PCR 30 cycles 200 bp DNA Ladder Mouse TATA box binding protein TBP 0 5 kb Mouse transferrin receptor Tfr 1 5 kb Mouse membrane glycoprotein Thy 1 2 6 kb M 1 2 3 B KOD FX Neo KOD FX A Long PCH PR M 1 2 3 M 1 2 3 M 1 2 3 M 1 2 3 M 1kb DNA Ladder 1 0 94C min 98C I oo GO i 0 Target Human globin 3 6kb 4C Hold OD280 1 Ei 00Ux 1 200 20C KFX 0 1 35 000 KOD FX Neo 1 0U 200UX1 x5 1 000 KFX 20 1 X5 140 000 POR Buffer for KOD FX Neo mM dNTP 200Ux1 x10 2 000 KFX 201X10 260 000 50 Il TA KOD TArget Clone Plu 10 TAK201 yeooo KOD FX Neo
37. gl uM Forward Primer uM Reverse Primer 3 gl Sample cDNA 2 ul Total reaction volume 0 ul PCR 98C 2 min 98C 10 sec 58C 10 sec 40 cycles 68C 1 min Melting curve step RNAi 1 THUNDERBIRD qPCR Mix OU 78 DD mRNA 3A mRNA GC PCR KOD SYBR qPCR Mix 1 5 mRNA
38. ldeas amp Chemistry T OO Y OO B QO L FF EE C NE UU LU AD 2015 sepPtemier 40 rand Ne item 1 PCR DNA PCR KOD Multi amp Epi eg HOT ITEM 5 PCR KOD SYBR qPCR Mix TECHNICAL REVIEW 7 KOD SYBR qPCR Mix PCR RNAi mRNA USER S NOTE 9 KOD SYBR qPCR Mix ChIP FLASH NEWS 10 TOYOBO LIFE SCIENCE KOD Multi amp Epi p 1 4 10 Santa Cruz Biotechnology CRISPR Cas9 System HOT ITEM KOD FX Neo TECHNICAL REVIEW 13 PCR IKOD FX NN es tem PCRH DNA PCR eratt OO KOD Multi amp Epi New G015 9 B01 6 3 18
39. lifescience toyobo jp 104 8345 17 10 TEL 03 6887 8819 FAX 03 6887 895 1 E mail order lifescience toyobo jp TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail tech_osaka toyODO jp URL http lifescience tOyODO CO jp NC A5232K 2015 9 23 000 1
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