『 レビス ® OVA‐IgG1 マウス 』取扱説明書
Contents
1. 1 2 0 1 1 1 ELISA 20 25 0 4m sec 302. 1 3 lt IgG 50u 1 3 10u 1 4 10u 1 5 CK 6 20 25 C 1 7 3
3. 100 ci 3 10041 TE 20 25 C 30 gt 8 TMB TMB 100k1 20 25 C 20 1M Hs804 1001 450nm 620nm 600 650nm 30 10
4. TMB 450nm 620nm OVA IgGi 5 ELISA
5. 160mg dL 160mg dL 2 100 100 10 000 PP PE 10 100 1 000 10 000 u 1 5 20 20 a 20 u 1 45 180 180 180 1 2 8C 35C 11
6. 3 300w1 5 25ml1 600 1 200rpm 10 3
7. Web Q amp A 20 25 C 2 10 mU ml 120 60 30 15 7 5 3 75 1 88 0 1 10 50 50 50 50 50 50 0 1 90 50 50 50 50 50 50 50 IgG 100 OVA 96 8 IgG 5041 101 20 25 C 1
8. 8 100u 1 9 20 25 C 30 10 3 lt 11 100u 1 CK 4 2 12 20 25 C 20 13 100u 1 14 450nm 620nm 600 650nm 7 3 75 mU7ml
9. 9 0 4m sec Web OVA IgGi OVA 6 B Anti OVA_HgG 1 200 mU ml D IgG E H 1M HzSO4 1 10x 8 1 7 5 10p 1
10. Web 10 OVA IgG 3 35 160mg dL
11. 2 8C B Anti OVA TgG1 1 200 mU m1 2 8 C C TIMB 2 8 C D IgG ED 2 8 C H
12. 4 10 100 Anti OVA lgG1 mU ml Tecan 13 OVA IgGi 1 88 120 mU ml ELISA IgG IgGi 5 2 C V 5 2 3 4 C V 5 2 2 IgGi OVA 92 5 103 2 3 R2 0 9994 0 9998 14 OVA IgGi
13. 2012 12 3 OVA IgGi Web Q amp Al 13 x 10cm 1 OVA IgGi 1 50 OVA IgG 0 96 2 2 8 C
14. Anti OVA TgGi 1 200 mU m1 C 120 30 120 60 30 0 5s0pl 0 1U ml Ka 6 9 x 107M 160ng ml D IgG 200u 1 C 100 E 200u 1 C 100 IgG 1 10 x 10 x 10 100ml 10 x 900ml 96 A OVA 96 OVA
15. 1 2 3 Q 1 A 1 Q 2 A 2 Q amp A 16 Web
16. 10 100u 1 100 500u 1 Eppendorf multipette plus 50u 1 100u 1 Vortex 600 1 200 rpm 96 Web 96 450 10 nm 620nm 600 650nm 8 20 25 C 2 6 B Anti OVA TgGi 1 200 mU m1l B
17. 6 3 IgG IgG 2 3 y 1 146 kDa IgG OVA ovalbumin OVA IgGi OVA IgGi anit OVA IgG1i 4 IgG OVA 1 OVA ITgGi 30
18. 27 2 U ml SD 1 92 U ml BALB c 8 3 OVA Alumina 20 mg ml OVA 50 ng ml v y OVA 0 1M Carbonate Buffer pH8 5 1 mg ml Saline 50 ng ml 0 2ml 1 2 3 15 Q amp A 1 2 3 4 5 6 7 1 88 mU m1 OD OD 3 4 6 CV
19. LM HzSO4 2 8 C 10 x 10 x 2 8 C 9 1 1 96 0 4m sec 30
20. OVA IgG AKRIE 040 Mouse OVA IgGi ELISA KIT AKRIE 040 8Shibayagi Gunma Japan 377 0007 1062 1 TEL 0279 25 0279 FAX 0279 23 0313 lt E mail gt syc info shibayagi co jp lt URL gt http www shibayagi co jp
21. G HH 0 8 16 24 32 40 12 1 X Log mU m1 Y Web ELISA mU m1 C 3 20 25C Abs 450 1620 nm 3
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