PrimeSTAR® Max DNA Polymerase
Contents
1. BF e v201312Da TaKaRa fe 07734319772. PrimeSTAR HS DNA Polymerase PCR PCR DNA Polymerase 3 5 exonuclease PrimeSTAR Max Premix 2x 625 ulIX4 Mg2 2 mM 2X dNTP Mixture 0 4 mM 2X Polymerase Chain Reaction PCR DNA Supercoiled pBR322 DNA Hind III digest
3. ADNA nicking exonuclease endonuclease ORF 20 C PCR PrimeSTAR Max DNA Polymerase PCR PCR PCR 1 Plasmid DNA PCR 4 kb 2 DNA PCR 0 5 1 2 3 4kb
4. NA as a template amplified fragment 4 kb Good amplification and performance were confirmed by PCR using hu man genomic DNA as a template amplified fragment 0 5 1 2 3 4 kb NOTICE TO PURCHASER LIMITED LICENSE P1 PCR Notice Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US 5 789 224 5 618 711 6 127 155 and claims outside the US corresponding to expired US Patent No 5 079 352 The purchase of this product includes a limited non transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser s own internal research No right under any other patent claim no right to perform any patented method and no right to perform commercial services of any kind including without limitation reporting the results of purchaser s activities for a fee or other commercial consideration is conveyed expressly by implication or by estoppel This product is for research use only Diagnostic uses under Roche patents require a separate license from Roche Further information on purchasing licenses may be ob tained by contacting the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA L15 Hot Start PCR Licensed under U S Patent No 5 338 671 and 5 587 287 and correspond ing patents in other countries M54 PrimeSTAR HS DNA Polymerase This product is co
5. PrimeSTAR Max DNA Polymerase Code No R045A Size 625 ulx 4 for 100 PCR reactions Shipping directly on dry ice Store at 20 C Storage Avoid repeated freeze thaw cycles Lot No Expiration Date For a detailed protocol refer to the product user manual Description PrimeSTAR Max DNA Polymerase offers extremely fast extension speed high fidelity high sensitivity and high specificity for PCR amplification The high priming efficiency of this enzyme and the addition of an extension factor shorten annealing time and extension time dramatically allowing high speed PCR In addition the antibody mediated hot start formulation prevents false initiation events i e mispriming during reaction assembly allowing reaction mixtures to be prepared at room temperature Content PrimeSTAR Max Premix 2X 625 ulx4 k Mg conc 2X 2 mM dNTP Mixture 2X 0 4 mM each Applications DNA amplification by PCR Purity Nicking endonuclease activity and exonuclease activity were not detected by purity testing with supercoiled pBR322 A DNA or A Hind Ill digested DNA PCR product A significant percentage of PCR products obtained using PrimeSTAR Max DNA Polymerase will have blunt ends Thus the obtained PCR products can be directly cloned into blunt end vectors If necessary phosphorylate PCR products before cloning PCR test Good amplification and sensitivity were confirmed by PCR using plas mid D
6. vered by the claims of U S Patent No 7 704 713 and its foreign counterparts PrimeSTAR is a registered trademark of TAKARA BIO INC Note This product is for research use only It is not intended for use in therapeutic or diagnostic procedures for humans or animals Also do not use this product as food cosmetic or household item etc Takara products may not be resold or transferred modified for resale or transfer or used to manufacture commercial products without written approval from TAKARA BIO INC If you require licenses for other use please contact us by phone at 81 77 543 7247 or from our website at www takara bio com Your use of this product is also subject to compliance with any applicable licensing requirements described on the product web page It is your responsibility to review understand and adhere to any restrictions imposed by such statements All trademarks are the property of their respective owners Certain trademarks may not be registered in all jurisdictions v201312Da PrimeSTAR Max DNA Polymerase Code No R045A Size 625 ul X 4 for 100 PCR reactions Shipping directly on dry ice Store at 20 C Lot No PrimeSTAR Max DNA Polymerase
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