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1. ML START 15 36 4 10 3 DNA DNA 4 10 2 PCR 6 1 2 3 4 5 4 11 125ng ul OD 260 280 1 75 1 85 1ug PCR dsrB 400bp amoA 270bp 16s rRNA 600bp 500bp PCR 1 DNA DNA
2. DNA DNA 2 DNA 3 DNA DNA DNA
3. DNA 4 4 1 DNA 3 DNA 4 4 1 ISBN4 87962 148 X 2 ISBN4 87962 149 8 ISBN 9784897069203 4 2 1 DNA DNA
4. DNA DNA gt 2 2011 2 17 2009 02 23 1 2010 2 4 ISO 2 2011 2 17 DNA DNA DNA gt
5. 3 5 cm 20 cm X20 cm 2 4 5 6 ss OS Ver 1 0 DNA 4 1 4 2 4 3 4 4 4 5 4 5 1 4 5 2 4 5 3 4 5 4 O 4 5 5 DNA E 2010 1 29 1 DNA PCR DGGE
6. DNA Q1 A1 Q2 A1 A2 DNA DNA DNA DNA 1 2 Q3 A1 A2 DNA DNA DNA DNA DNA 48 6
7. PCR PCR PCR 12 DNA dsrB 16s rRNA 96 PCR 4 amoA 6 1 5m 1 PCR 1 PCR 1 3 PCR 5 PCR 1 5m 1 5m 96 PCR 100ul 100ul dsrB 16s rRNA
8. 2 55
9. 3 4 1000ml 121 20 5 1000ml 500 40 1 50 6 1000ul Amp 500ul 1000ul 500 3 100ug ml 0 0049 6 7 5mm 8 1 9 4
10. 15 50 C 16 20 4 14 DNA DNA 50ml 2x SSC 0 1 SDS 40ml 20x SSC 4ml 10 SDS 0 4ml 35 6ml DNA 1 40ml 42 1 2 3 4 5 6 7 8 9 1000m 2x SSC 0 2 SDS 300ml 500m 20x SSC 30ml 10 SDS 6ml 264ml 1000ml 0 2x SSC 0 2 SDS 900ml 1000m 300ml
11. 11 100ul 40ul DNA DNA 12 DNA 2mm DNA 13 10ul 10ul 20ul DNA 14 50
12. 001 49 001 2 E mail 50 002 2 1 C m C mg L g mg g dry 9 6 mg D mg g
13. 1 2 3 4 5 6 350C 1000m 800ml 1000ml 1000ml 300ml 1000m 900ml 3 300ml DNA DNA
14. 4g 250ml 1 Methy 2 pyrrolidinone 250ml 1000 5 10 10ml 1M pH 8 0 27 5ml 50m 1000 50m 1M pH 8 0 27 5ml 1 37 7 DNA
15. 100 6 102 7 160 07 1 7 160 10 1 MEISTER 2 8028 21 1 1 4615 02 DNA BM THC 1 5 064 08 1 10
16. RNA PCR DNA DNA DNA 1 DNA 1 DNA DNA DNA 1 3 23 DNA DNA 2 2 1 DNA DNA dsrB amoA 16s rRNA RNA DNA PCR DNA DNA DNA
17. URL TA TA http www promega coJp jp jp_tech jp_manuals pgem t pdf PCR 4 10 DNA 4 10 2 PCR PCR DNA DNA 96 1 5ml 37 42C 20 SOC medilum TA
18. 50C 50 C 94 65C 65C 4 3 DNA 4 10 2 PCR 20 HEAT LOW 41 5 20 1 4 12 1 DNA DNA 20ul 5ul 1
19. DNA A DNA 1 A 1 5m 96 PCR 2 DNA 1 100ul 50ul 3 4 DNA 1 C PCR DNA
20. 22 22 DNA Agilent Scan Control URL http www chem agilent com Library usermanuals Public G2505 90021_ScannerC_User pdf DNA DNA DNA DNA 3 Agilent Scan Control DNA
21. 20 11 10 12 DNA 1000ml 3 13 DNA 5 14 DNA 15 DNA 96 21 90g 2 DNA
22. 300ml AGC 71 008 014 1 1000ml AGC 71 008 018 1 500ml AGC 71 023 012 1 TPX 100ml 6 219 01 1 TPX 300ml 6 219 03 1 TPX 500ml 6 219 04 1 10ml 1 4908 05 1 AGC 82 064 004 1 BIO BIK 6 8626 03 2 5057 01 1 6 489 01 L 350 L 350
23. PCR 1 5ml 15ml dsrB 16s rRNA 1 5ml 2 amoA 15ml 1 1 5ml 2 DNA DNA URL http www Ims cojp commodity img si nd ND 1000ver37 pdf PCR 1 59 4 10 1 PCR 4 10 1 PCR 1 PCR 1 3 Buffer PB PCR 5 1 5 dsrB 16s rRNA
24. 1 5m 96 PCR PCR 4 30 20 Amp 100ml 2 121 C 20 5ml 3ml 15ml Amp 1 5ul 10 100ug ml 24 1 2 3 4
25. 14 ug p mol ug ug DNA ng ul x 20 DNA 1000 p mol ug p mol ul x 1000 DNA ng ul 4 12 3 DNA DNA 4 12 2 DNA 2 1 DNA 50 2 20p mol ug 4 13 1 5ml
26. mg g dry mg g 51 003 1 DNA DNA 1 ki 3 DNA DNA DNA DNA DNA DNA DNA DNA DNA DNA 52 004 DNA 2 DNA 1 DNA DNA DNA DNA g DNA DNA DNA DNA DNA ug ul DNA ug OD 260 280 1 DNA DNA 53 00
27. 16 a 96 4C 1 4 9 1 DNA 4 9 DNA DNA URL QIAprepR Miniprep QIAprep Miniprep Handbook http www qiagen com products plasmid qiaprepminiprepsystem qiaprepspinminiprepkit aspx Tabs t2 4 10 2 PCR 28 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Amp
28. FM 120F MHE 132AJ TOMY SX 300 Direct Q UV Direct Q UV 1 9284 03 2 2 6863 01 TOMY PMC 060 TOMY MX 301 TOMY TMA 300 AR015 24 Gold 96 well GeneAmp PCR System 9700 9700G Mupid exU ADVANCE EXU 1 BioDoc It System 8 inch Monitor 100V with LMS 20 Transilluminator with Digital Printer 97 0170 03K NanoDrop 1000 LMS ND 1000 TOMY CC 105 TOMY TU 1000 GCD 051X TCA 72 B
29. DNA DNA 96 PCR DNA PCR PCR PCR PCR PCR PCR PCR PCR PCR PCR DNA DNA DNA DNA 2 2 1 MSDS 2
30. DNA PCR Q1 A1 A2 Q2 A1 A2 Q3 A1 A2 PCR PCR Buffer dNTP DNA DNA PCR PCR PCR Q1 A1 Q2 A1 A2 DNA DNA
31. 5 2ul 1 5ul 6 Blank 7 8 4 12 1 DNA 22 DNA 1 5ul 40 9 Measure 10 DNA 11 12 12 Print Screen DNA Print Report 13 DNA
32. 2 4 9 DNA 3DNA 001 002 003 DNA 004 DNA 005 006 1 2 0 NN oa OO ND 10 10 10 10 11 12 12 12 12 13 14 15 16 17 0M N N
33. 8 Close 9 I Tools Laser Auto ON OFF Setting II Turn Laser OFF Now 10 File Exit Agilent Scan Control 11 I DNA IT DNA 6 4 16 24 2 4 DNA
34. 30 96 PCR 3 amoA 5 5 DNA PCR 12 PCR 100ul 100ul 96 PCR 1 6 96 PCR PCR 7 DNA 96 PCR PCR 13 PCR DNA PCR DNA 4C 1 1620 8 PCR PCR 1 5 14 9 PCR 96 PCR DNA 10
35. 1 9178 02 KGT 4056HC FM 120F 11 96 96 1 6607 11 1 4603 11 TOMY LX140 TOMY TS 35LB A138 96 S1596 04 1 9284 03 FM 120F 357590 Direct Q UV Direct Q UV
36. 2 5674 01 1 5 235 03 1 TPX 1000ml 6 236 08 1 TPX 2000ml 6 236 09 1 250ml IWAKI 72 086 011 1 16 10ml 5ml 2 DNA 1000ml 1000ml 300ml 5 001 16 1 8mm x 51mm 1 5407 06 3 KER30 10
37. 1 12 1 5m QIAprep 100u 45 10 4 10 2 PCR 29 24 25 26 DNA PCR 4 10 DNA PCR 500bp 2 4 9 DNA 15 1 DNA 4 DNA DNA DNA 23
38. 2 3 IE 1 2 IE 10 cm 4 cm
39. 1 9284 03 FM 120F 357590 Direct Q UV 12 DNA DNA 4 5 Direct Q UV 2 2 6863 01 TOMY PMC 060 TOMY MX 301 TOMY TMA 300 AR015 24 NanoDrop 1000 LMS ND 1000 TOMY CC 105 1 DNA DNA PCR 96 PCR PCR 96 96 PCR 8 96 PCR
40. DNA 2x SSC 0 2 SDS 500 15 DNA 0 2x SSC 0 2 SDS 500 15 DNA 0 2x SSC 0 2 SDS 500 15 7 DNA 0 2x SSC 20 10 DNA
41. 1 500m 500ml 500ml 2 LB 500ml 5 3 500ml 20 4 500ml 4 5 500ml 121 20 6 5 7 1 2 30
42. Biding Matrix 600 hl SPIN Filter 14 000Xg 1 SPIN Filter Biding Matrix lt SPIN Filter 300 pl SEWS M SEWS M J 14 000Xg 1 0 SPIN Filter ee Matrix SEWS M 14 000Xg SPIN Filter Catch Tube SPIN Filter 3 50 hl DES 14 000Xg 1 SPIN Filter DNA 20 E
43. 0 2x SSC 20 11 DNA 0 2x SSC 12 DNA 96 90g 2 DNA 0 2x SSC 4 11 DNA 13 96 DNA 5 14 DNA 43 4 15 DNA DNA
44. 3 1 5m 10 1000u 1ml 4 1 5m 4 4 1 DNA DNA Gold 96 well GeneAmp PCR System 9700 9700G DTU Mini B 1120 A MIR 162 BR 21FP MR TOMY MX 301 TOMY TMA 300 AR015 24 2
45. 9 6ml 1 50ml 39 6ml 3 1000u 1M pH 8 0 400ul 4 50ml 20 10mM 6 1 1 5ml 10 100ul 50ul 2 1 5m 20 30 20 30 C 6 3 1 4 4C 3 1 Amp 100mg ml 1 4 Amp 15
46. 260 2 20 200 200 1000 cn 2 ml RA r SAT 4 5 4 O Lysing Matrix E Tube 4 0 ml Iwaki Glass FastDNA SPIN Kit for Soil a 0 0 5 g Lysing Matrix E Tube DNA 8 C 4 5 5 DNA 1 Lysing Matrix E Tube 978 pl Sodium Phosphate Buffer 122 MT Buffer Sodium Phosphate Buffe
47. 20pg ul 600ul 10 20pg ul DNA 200ul 1 5ml 200ul 4 4 10 DNA DNA DNA DNA dsrB amoA 16s rRNA PCR PCR DNA 4 10 1 PCR 1 2 3 4 DNA 96 PCR 1 5m 96 PCR PCR
48. 9 16 DNA DNA 16 19 1 1 59 DNA 2 1 59 17 PCR 1 PCR PCR dsrB 400bp amoA 270bp 16s rRNA 600bp 500bp 2 PCR 1 18
49. DNA2 DNA1 5 TACGTGTCAGTAGCATGATCGTG 3 DNA2 5 CGATGGCCCACTACGTGAACCATCA 3 DNA1 5 ACGTGTGCACTAGCTCTAOCTAGG 3 DNA2 5 CGATGGCCCACTACGTGAACCATCA 3 5 DNA 0 2mIPCR PCR PCR 6 PCR 0 2mIPCR DNA 4C 1 7 1 5ml 10ul PCR 10ul 2ul 1 5 8 5 9 500bp PCR 4 10 1 PCR 18 500bp PCR 4 10 6 PCR 2 4
50. 200ul 1000 1 5m 2ml 0 2mIPCR 1 5m 15ml PIPETMAN Ultra U200 F21025 1 PIPETMAN Ultra U1000 F21026 1 10ul 124 10S 20ul 124 20S 100ul 124 100S 200ul 1252 703CS 1000ul 124 1000S 1 7 ml SnapLock Microtube Sterilized Maxy
51. 003 DNA 004 DNA 005 006 7 001 2 MSDS 3 3 DNA PCR 3 DNA DNA 4 DNA DNA DNA DNA DNA
52. 2 10ml 15ml 5ml 3 1g 15m 4 1 10 5 6 15m 7 4 8 15ml 9 5ml 1ml 1 5m Amp 1 5ml 10
53. 2 7915 01 2 6122 01 1 Hn 7 gn amp 4 7
54. 24mm x 60mm C024601 7 5mm x 25mm 7 216 03 4 8mm x 51mm 1 5407 06 19 1000ml 300ml 2ul 10ul 20ul 100ul 200ul 1000ul 10ul 20ul 100ul 200ul 1000 1 5m 3 TPX 1000ml 6 236 08 1 TPX 300ml 6 236 06 1 PIPETMAN Ultra U2 F21021
55. 5ml 3ml 15m Amp 1 5ul 10 100ug ml 37 7 4 9 DNA 16 96 4 9 DNA 15 10ul 10ul 15m 15ml 37 16 20 2m 2ml 2ml 6800g 3 1000ul
56. PP 8 7479 01 15 1 5ml DNA DNA 1000ml 2000ml 250ml 2000ml 7 164 04 1 1 IO 1 1 BM DGP 350 S 1 1 8063 01 1 9 5618 04 2 2 Bind ET System Complete lt Ethidium Removal System gt 2350 1 1 4562 03 10 1 7432 01 1
57. Project Summary Report 46 4 17 DNA PCR PCR PCR OK PCR OK DNA DNA DNA DNA 47 5 2 DNA
58. Vortex Mixer MT Buffer 2 FastPrep 24 Instrument Lysing Matrix E Tube 5 5 30 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 29 Lysing Matrix E Tube 14 000Xg 30 2 ml 250 PPS 10 14 000Xg 3 4 0 ml Biding Matrix Suspension 1 ml Biding Matrix Suspension NN 2 3 300 Biding Matrix SPIN Filter
59. DNA 16 96 DNA 5 17 DNA 1 1 96 96 96 DNA 38
60. 2 3 4 5 4 6 3 002
61. PCR PCR 96 PCR 11 200u PCR 1 1 5m dsrB 16s rRNA 2m amoA 15m 12 PCR 1 3 13 PCR 5 14 ei 10ul PCR 10ul 1 5m 15 em PCR PCR 4 4 C 1 16 1 10ul 2ul
62. DNA 500 15 30 DNA DNA 8 DNA DNA 9 DNA 20 10 DNA
63. IT 42C 50 1 5m 2 TV 1000u SOC medium 890ul 3 V 37 C 1 30 I 10 II 100ul 100ul TV 37C 16 lt 20 PGR 200ul
64. 1500ul amoA 2500ul 3M dsrB 16s rRNA 10ul amoA 20ul 1 5 QIAquick PCR 650 17900g 1 PCR 4 5 6 QIAquick PBuffre PE 750ul 5 6 10 QIAquick 5 35 11 QIAquick 1 5ml 60ul 12 3 13 5 14 QIAquick 1 5ml 15 DNA PCR A 125ng ul DNA
65. 2 1 1 2 3 5 1 20 cm 3 4 mm 50 mm 4 1 6 775 03 6 7 8 2 8 cm 40 cm 1 cm 1
66. 2 4 8 DNA DNA DNA DNA DNA DNA DNA 22 DNA DNA 4C A DNA 5 6 5 DNA 6 4 10 2 PCR B DNA DNA 20ul 4 DNA 20u 2 4
67. DNA DNA DNA 1 DNA DNA 1 PCR 2 PCR 3 DNA DNA DNA 1 DNA 2 DNA 3 DNA DNA DNA 001 002 003 DNA 004 DNA 005 006 1 DNA DNA DNA
68. 3 20x SSC 9ml 10 SDS 18ml 873ml 1000m 10ml 0 2x SSC 600ml 1000m 300m 2 20x SSC 6ml 594ml DNA 2x SSC 0 1 SDS 50ml DNA 10 2x SSC 0 1 SDS DNA
69. 1 6071 01 4 DNA DNA DNA 96 96 DTU Mini B 1120 A AccuBlock Mini Cmpact Digital Dry Bath Incubator BM D0100 MIR 162 DNA G2565CA 1 4603 11 TOMY LX140 TOMY TS 35LB A138 96 S1596 04
70. 2 6863 01 TOMY PMC 060 TOMY MX 301 TOMY TMA 300 AR015 24 Mupid exU ADVANCE EXU 1 BioDoc It System 8 inch Monitor 100V with LMS 20 Transilluminator with Digital Printer 97 0170 03K NanoDrop 1000 LMS ND 1000 1 9178 02 KGT 4056HC MDF U482 10 2 PCR DNA DNA DNA DNA 3 DNA
71. 1 5 17 5 18 S5ul 12ul 8 1 5 20ul 14 19 DNA 15 16 20 PCR 17 1 12 PCR A dsrB 16s rRNA 1 4 DNA 10ng ul 0 5 ul 5ng 1 7 ul 2x KOD Buffer 50 ul 200 ul 50 ul 31 2mM dNTPs 20 ul 80 ul 20 ul DNA 1 100uM 0 3 ul 1 2 ul 0 3 ul DNA 2 100uM 0 3 ul 1 2 ul 0 3 ul DNA KOD 2 ul 8 ul 2 ul 27 ul 108 ul 29 ul Total volume 100 ul 400 ul 100ul B amoA 1 6 DNA 40ng ul 0 5 ul 20ng 3 ul 2x KOD Buffer 50 ul 300 ul 50 ul 2mM dNTPs 20 ul 120 ul 20 ul DNA
72. 16s rRNA 94C 2 98 C 10 58 C 30 x 30 68 C 1 4 C dsrB 94 C 2 98 C 10 58 C 30 x 33 68C 1 4 C amoA 94 C 2 98 C 10 58C 30 x37 68C 1 4 C 14 1 5 0 4 4 18 4 1 1 1 596 4 2 2 3 135V 30 33 3 5cm 15
73. Geometry Information Manual Ft File Save grid grid csv feat csv 2 X 3 Project Project Extraction Set Configuration Grid Name Browse Grid File 10 grid csv Project Extraction Set Configuration Protocol Name Project Project Start Extracting
74. DNA DNA DNA DNA EX Ex Taq Hot Start Version TaKaRa RR006A Distilled Water Deionized Sterile 5 TA Amp 1M pH 8 0 7 7 el DNA 2 PCR DNA DNA KOD PCR 316 90101 pGEMR T Easy Vector with DH5 w TOYDH5 QIAprep Spin Miniprep Kit QIAGEN 27104 LB 393 00881 LB 396 00871 016 10373
75. PCR 20 125ng ul B B PCR 500ng 4 9 1 PCR PCR 16 15 PCR 100ng 1 5m 1 100ng PCR 10mM PCR 10mM pH pH 10mM PCR 10ul 10mM pH pH 18 2ul
76. 85 C 30 8 I 2ml 2ml DNA I 20400g 1 DNA 20800g 20800g 20400g 2ml 39 IV 1000ul 300ul V 9 1 5ml 5 10 1 5m 1 5ml 2 11 20400g
77. DNA DNA 7 0 2mIPCR DNA1 10uM 25ul DNA2 10uM 25ul Total volume 50ul 7 DNA DNA1 5 AGACGATCATGCTACTGACACGTA 3 DNA2 5 ATACGTGTCAGTAGCATGATCGTC 3 DNA1 5 ACCTAGTAGAGCTAGTGCACACGT 3 DNA2 5 AACGTGTGCACTAGCTCTACTAGG 3 1 DNA DNA 95 C 5 55C 10 4 C 0 2mIPCR 96 2 DNA TA 0 2mIPCR 2 2x Rapid Ligation Buffer T4 DNA Ligase 5ul pGEM T Easy Vector 1ul 2ul T4 DNA Ligase 1ul 1ul Total volume 10ul P
78. 1 PIPETMAN Ultra U10 F21022 1 PIPETMAN Ultra U20 F21023 1 PIPETMAN Ultra U100 F21024 1 PIPETMAN Ultra U200 F21025 1 PIPETMAN Ultra U1000 F21026 1 10ul 124 10S 20ul 124 20S 100ul 124 100S 200ul 1252 703CS 1000ul 124 1000S 1 7 ml SnapLock Microtube Sterilized MaxyClear 20 1 5m
79. 4C DNA DNA DNA DNA 23 6 4 9 DNA DNA DNA 2 24 2 2 TA PCR TA PCR DNA DNA
80. Amp 20 C 2 1 20 X Gal 15 2 10ml 15ml NIN 5ml 8 3 X Gal 100mg 15m NN 4 1 10 5 5ml X Gal 1m 1 5ml 6 20 1 500ml 500ml 1000ml 2 LB 500ml 5 3 1000ml 4 750 3
81. 10ul 20ul 100ul PCR 35801 Silicone Expansion Mat for 96 well PCR Plate AxyMat CM 96 EXP 20 2 IO 2 1 BM PCR 8C PCR 2512 922B 1 PIPETMAN Ultra U10 F21022 1 PIPETMAN Ultra U20 F21023 1 PIPETMAN Ultra U100 F21024 1 13 200ul 1000ul 10ul 20ul 100ul
82. 4 4 1 DNA DNA 4 2 Y FastDNA SPIN Kit for Soil MP Miomedicals LLC 4 3 v FastDNA SPIN Kit for Soil SEWS M 100 ml FastPrep 24 Instrument 1 5 2 0 ml 14 000Xg x 4 5 5 4 1 4 5 2 4 5 3 vv 100 ml 1 5 ml
83. DNA dsrB amoA 16s rRNA DNA 4 1 5ml 3 1 DNA dsrB amoA 16s rRNA DNA DNA 500ng 16ul DNA 2ug 16ul 2 1 5m 1 3 1 DNA 4 2ul 10x labeling solution 2ul 5 2ul Cy3 ULS Cy5 ULS 2ul 6 20u 15ul 20 7 1 5m
84. Feature Extraction URL http www chem agilent com Library usermanuals Public G4460_90034 FeatureExtraction_User pdf DNA DNA Feature Extraction gal 25 DNA 26 25 gal DNA DNA DNA gal 45 1 2 3 4 5 6 7 8 9 10 11 12 13 14
85. IV Scan Region Agilent HD 61x21 6mm V Resolution 5um VI TIFF 20 bit 2 3 4 44 TR PMT G PMT 10096 Output Path XDR No XDR 5 Scanner ready Scan Slot m n 23 23 Scan Slot m n m n 4 1 Scan Progress 7
86. 1 5 19 PCR 5 20 5ul PCR 1 5 20ul 4 10 1 PCR 21 DNA 22 4 9 3 DNA 17 20 DNA I 10 85 TROTOR COMP TAIR HEAT HIGH 15
87. DNA ExpressHyb Hybridization Solution 636831 Distilled Water Deionized Sterile 316 90101 arrayCGH Labeling Kit Cy3 Cy5 ULS EA 005 20x SSC 15557 044 10 SDS 24730 020 3 1 2000m 1960ml 2000ml 2 50ml 50X TAE 40ml 7 3 2000m 20 1 10mM 1 25ml 30ml 50ml 25ml 30ml 2 10ml
88. 1000ul Buffer P1 250ul 1000ul Buffer P2 250ul 4 6 1000ul Buffer N3 350ul 4 6 2ml 17900g 10 1000ul QIAprep QIAprep 17900g 1 1000ul 1000ul Buffer PB 500ul QIAprep 12 13 1000u Buffer PE 750ul QIAprep 12 13 12 QIAprep 1 5ml 60ul QIAprep QIAprep
89. 200ul 96 A 10 PCR 10 96 PCR A 11 1 PCR 11 5 10 PCR 1 11 5 10x EX Taq Buffer 5ul 57 5ul 10ul 2 5mM dNTP mix 4ul 46ul 8ul DNA1 10uM 11 0 5ul 5 75ul 1ul DNA2 10uM 11 0 5ul 5 75ul 1ul 27 DNA EX 0 2ul 2 3ul 0 5ul 39 8ul 457 7ul 79 5ul Total volume 50ul 50ul 11 DNA2 DNA1 5 TACGTGTCAGTAGCATGATCGTC 3 DNA2 5 CGATGGCCCACTACGTGAACCATCA 3 DNA1 5 ACGTGTGCACTAGCTCTACTAGG 3 DNA2 5 CGATGGCCCACTACGTGAACOCATOA 37 I 96 PCR PCR 96 I EE
90. X Gal TaKaRa 9031 NN 045 29192 1M Tris HCI pH 8 0 100ml 312 90061 Agarose S 312 01193 6x Loading Buffer TaKaRa 9156 50X TAE 313 90035 100bp DNA BRG 100 02 EtBr Solution 315 90051 99 5 Wako 057 00456 DNA DNA DNA KOD FX TOYOBO KFX 101 Distilled Water Deionized Sterile 316 90101 QIAquick PCR Purification Kit QIAGEN 28104 3M Sodium Acetate 316 90081 6 1M pH 8 0 el
91. 10ml 25ml 50ml 1 5m 1 5m MCT 175 C S ECO 96 1521 902W 1 5 2 0ml 50 2572 502N 10ml BD Falcon 357551 25ml BD Falcon 357525 50ml BD Falcon 357550 BM DGP 350 S 1 1 8063 0
92. 3 2 5 3 DNA DNA LOW 5 4 20ul DNA 20ul 20ul 3 5 DNA 94 3 6 DNA 5 7 DNA 5 8 20400g 1 9 DNA 20ul 20ul 65 C 20ul 15 2 10 DNA
93. 4 12 DNA DNA Cy3 Cy5 DNA URL DNA DNA http www_funakoshi cojp data datasheet KRA EA 005 pdf 4 12 1 DNA DNA 2 3 DNA DNA 85 1 5m
94. 15 16 NS DNA DNA 26 26 DNA 0120 477 111 Feature Extraction File New Standard Project Project Project Project Explorer Project Explorer tiff Edit Grid Mode Create Edit Grid Gene List Type Gene List Protocol for Grid Mode OK gal
95. 6 6685 01 5 235 03 IWAKI 11 039 008 KER30 10 1 2 7915 01 7 5mm x 25mm 7 216 03 1 1 2 6122 01 3 DNA DNA TPX 500ml 500ml 18 6 219 04 1000ml 1 TPX 1000ml 6 219 05 2
96. DNA Figure DNA DNA I DNA Scan slot IT Slot ID DNA 3 Channels R Red Cy5 G Green Cy3 DNA Cy3 G Cy5 R G
97. 1 10uM 0 3 ul 1 8 ul 3 ul DNA 2 10uM 0 3 ul 1 8 ul 3 ul DNA KOD 2 ul 12 ul 2 ul 27 ul 162 ul 29 ul Total volume 100 ul 600 ul 100ul C 1 4 DNA 20pg ul 0 5 ul 10pg 1 7 ul 2x KOD Buffer 50 ul 200 ul 50 ul 2mM dNTPs 20 ul 80 ul 20 ul DNA 1 100uM 0 3 ul 1 2 ul 0 3 ul DNA 2 100uM 0 3 ul 1 2 ul 0 3 ul DNA KOD 2 ul 8 ul 2 ul 27 ul 108 ul 29 ul Total volume 100 ul 400 ul 100ul D DNA dsrB DNA 1 5 CAACATCGTYCAYACCCAGGG 3 DNA 2 5 TTCTCGTGTTCCGTTTGTACTCTAAGGTGGAGTGTAGCAGTTACCGCA 3 16s rRNA DNA 1 5 GCCCTACGGGAGGCAGCAG 3 DNA 2 5 TTCTCGTGTTCCGTTTGTACTCTAAGGTGGACCGTCAATTCCTTTRAGTTT 3 amoA 32 DNA 1 5 TTCTTCTTTGTTGCCCAGTA 3 DNA 2 5 TTCTCGTGTTCCGTTTGTACTCTAAGGTGGACTGAYTGGGCYTGGACAT 3 DNA 1 5 TACGTGTCAGTAGCATGATCGTC 3 DNA 2 5 CGATGGCCCACTACGTGAACCATOCA 3 DNA 1 5 ACGTGTGCACTAGCTCTACTAGG 3 DNA 2 5 CGATGGCCCACTACGTGAACCATOA 3 13 PCR
98. 10 2 PCR 11 TA 0 2mIPCR 2 TA 2x Rapid Ligation Buffer T4 DNA Ligase 5ul 26 12 13 14 pGEM T Easy Vector 1ul 2 2ul T4 DNA Ligase 1ul 1ul Total volume 10ul TA 10ul DH5 w 100ul SOC medium 890ul Total volume 1000ul I 10ul 10ul 100ul 1 5m 20 1 5ml
99. 2 7915 01 10ml BD Falcon 357551 5ml BD Falcon 357543 ei 2 6122 01 1 3 i 1000ml IWAKI 71 001 018 1 100 5 101 1 102 2 1 7 160 07 1 1 4615 02 DNA
100. TPX 1000ml 6 236 08 1 TPX 300ml 17 250ml 25ml 50ml 1 6 236 06 250ml IWAKI 72 086 011 1 25ml BD Falcon 357525 BM 1 1 1 10 5 1 50ml DGP 350 S 1 8063 01 6 713 07 1 4562 03
101. 1 12 1 5ml 13 8 20400g 1 14 1 5m 1 5ml 15 12 16 20400g 1 17 1 5m 18 20ul DNA 19 23ul 20 20u 20ul 20 21 20ul DNA 20ul 1 5m 22 21 D
102. 1 1 6 713 07 1 1 IO 1 ECO 96 1521 902W 1 7 227 01 1 1 4562 01 10 6 6685 01 5 21 50ml 4 6 6 6689 01 10 5 235 03 1 2 5674 01 1 88 2 1493 01 1 50ml IWAKI 11 039 008 KER30 10
103. 5 2 1 DNA 2 DNA DNA 1 T E mail 006 1 DNA DNA
104. A dsrB 16s rRNA 10ng ul amoA 40ng ul 2 DNA 4 10 1 PCR DNA 1 DNA DNA 2 1 5m 96 PCR 3 4 3 DNA 5 20 DNA 2 3 2 3 6
105. CR I 10 0 2mIPCR 20ul 18ul 3 2u 1 5 5 IT I PCR 8 0 2mlPCR PCR 9 PCR 500bp PCR 25 PCR 100ul 90ul 15 PCR 8 PCR 1 1ul 10x EX Taq Buffer 10ul 10ul 2 5mM dNTP mix 8ul 8ul DNA1 10uM 9 1ul 1ul DNA2 10uM 9 1ul 1ul EX Taq 0 5ul 0 5ul 78 5ul 79 5ul Total volume 100ul 100ul 9
106. Clear MCT 175 C S 2ml 95170 0030 120 094 0 2 ml PCR Tube with Dome Cap Thin Wall Non Sterile MaxyClear PCR 02D C ECO 96 1521 902W 1 5 2 0ml 50 2572 502N 15ml IWAKI 14 11 039 004 300ml 1000ml 500ml 100ml 300ml 500ml
107. DNA 3 DNA 1M pH 8 0 1 Methy 2 pyrrolidinone anhydrous DNA 20x SSC 10 SDS 1M Tris HCI pH 8 0 100ml 312 90061 Agarose S 312 01193 6x Loading Buffer TaKaRa 9156 50X TAE 313 90035 100bp DNA BRG 100 02 EtBr Solution 315 90051 99 5 Wako 057 00456 Wako 194 04352 1M pH 8 0 1M pH 8 0 1 Methy 2 pyrrolidinone anhydrous 99 5 SIGMA ALDRICH 328634 1L 4 DNA
108. DNA DNA DNA DNA 2 DNA 1 DNA 400ml 2 DNA 20ul DNA 20ul 3 DNA L DNA DNA 1 4 DNA 1 59 DNA 1 59 5 DNA 20 DNA 6 1000m 500ml 7 OD 1 5 DNA 8 15
109. I T 10 96 PCR IV 96 a 37 C 16 20 V 96 PCR DNA PCR PCR 15 PCR 4 10 1 PCR I PCR 96 PCR DNA IL 10u PCR 10ul 96 PCR 10ul 2ul 10ul 10ul 10 IV 1 59 V 500bp PCR 2 96 PCR 1 12 500bp PCR
110. NA 19 23ul 21 20ul 3ul 4 12 2 DNA DNA DNA DNA 4 10 2 PCR DNA 1 10ul 3ul 2 MicroArray 3 OK 4
111. URL http www mupid com 19 URL http www funakoshi cojp 34 4 10 2 PCR f PCR URL PCR PCR QIAquickR Spin QIAquick Spin Handbook http www qiagen com products dnacleanup gelpcrsicleanupsystems qiaquickpcrpurificationkit aspx Tabs t2 1 2 3 4 5 6 7 8 9 1 5ml 4 10 1 PCR PCR 4C 1 5m QIAquick PCR
112. V KK IE Inside Ekman grab 1 50 mm 44 mm 1 23 cm 4 2

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