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Contents
1. 100 10 O 2014 04 09 O 2014 04 09 000000 00000 E E 00000 000 00000 0000000000 1000 0 GSC UI UI 0000 0 000 0000000 100 00 0000000 0000 0 000000 100 00 00 00 1000 0 1000 100000G5100000000000000 V shi ng Buffer 10x DO 00 00 00 DD pH 6 7 6 9 000000 00000 000000000000 00000 100 1000 0 10 000Q0000 00000G6010000000000 0000000 0000 0 utu 10000 000000 00000 1000 10000 1000 100000G5100000000000000 Substrate Conponent A DO DD U 00 O 00 pH 9 0 10 34 00000000 Cell Proliferation ELISA BrdU Cheml unnnescence 100 10 O 2014 04 09 O 2014 04 09 000000 00000 E E 00000 0002. 100 10 O 2014 04 09 IN 0 2014 04 09 1000000000 10000000 0000000000000 00000 00000000000 00000 0000000 10000 00000 10000 0000000 ubt 000000 10000 000000000 00 0 Substrate Conponent B 0000 EL 100000000 000000060 0 00000000000 00000 00000000000 00000 Im 000000000 0000 000 1 6000000000000 000000000 275 ny 0000 48h 0000000000000 EG0 0000000 0000 33 22 nyi uuu 1000 48h 1 000000000 00000000 00000000000000000 10000000000 10000 00000000 000 0 000000000000 00 625 ny 0000000000000 EG0 0000000 0000 120 nyi U B D IU 0000 72h 24 34 00000000 Cell Proliferation ELISA BrdU Cheml unnnescence 100 10 10 0 2014 04 09 JOO 2014 04 09 100000000 100000000000 0 00000000000000 IL 0000000000000 NNNNA E I I EE 00 00000000
3. 0200000 31 34 00000000 Cell Proliferation ELISA BrdU Cheml unnnescence IOO 10 O 2014 04 09 O 2014 04 09 100 0000000000000 100 0000000 100 000000 100 00000 1000000000000 000 1000000000000 0000000000000000 1000 0 Substrate Conponent A 0000 000 000 000 I 0000000 000000000000 000 HUHUHUDHUULHURU 0 000000000000 ANO D0O00dg0 00d 0D 0000000000000 UI OOOOOZUOUOUOOOOOOOUOOUOCU 100 I HHHHHEHHHHHUEUURD u 000 iugugggpupuuiuluuub 0 00 100000000000 000 000000 000 100000000000 UI 32 34 00000000 Cell Proliferati on ELISA BrdU Cheml unnnescence
4. 100 10 2014 04 09 100 2014 04 09 1000000000 000 1000000000 1000 000 IOD 000000000 100 0000000000000 000 0000000 000 000000 000 00000 0 0 0 0 000000 0 000 000 00000000000 0000000000000000 10000 Substrate Conponent B 0000 000 IUD 000 Odd Do gd 0 DO Dd 0000000 0000000000000 0000000 100000000000 000 0 000000000000 000 100000000000 000 000 IUUUUUDUDOUUUDUUDUDUUDUUUDUDU 000 0 000000000000000 000 100000000000 000 33 34 00000000 Cell Proliferati on ELISA BrdU Cheml unnnescence 100 10 OO 2014 0409 2014 04 09 EJ EJ E 100 000 00000000 0 0000000 00000000000 r 0 0 0 U 20000 r3 rp ETE DO DO r3 r3 DD 00000000000 000000000000 DO L3 r3 1e 100000 EJ L3 r3
5. 10 57102 0000009 100000 00 9 60 70 00 61 JO 1000 000 000000000000000 0000000000 000000000000 000 1000000000 000 0000000000 00000 000000000 EJ ET EJ EJ EJ T e a E EJ 7 a EJ B a c a r3 ES _ HE r3 r3 r3 aa r3 um 7 oog r3 r3 Do r3 r3 r3 m rd DO r3 r3 r3 ON EE EJ EJ EJ Do o OO OOO QE Hm oo moo mo OOO DD EEC oo EC mo EEE QE moo moo mo mo Bottle 3 Anti BRDU POD 28 34 10000000 Cell Proliferation ELI SA BrdU Cheml unnnescence IRR 10 D 2014 04 09 1 2014 04 09 0000 000 000 000 1000000000000000000U0000000000 EJ EJ EJ 100 000 EE E ET E EJ 020
6. Ol 000000000 00 0 0005 10 000000 DO DD DOLO 0000000000000 10000 00000000 10000 0000 10000 0000 10000 000000000 0 0000 IR Sucr ose 100000 0000000000000000000 0000 00000000 000000000000000 100000 0000000000000000000 0000 00000000 000000000 0 0 0000 00 Sucr ose I00000 00000000000000000000M00090 00000000 000000000000000 100000 0000000000000000000 0000 00000000 000000000 00 Sucr ose 10000 000000000000000 10000 0000 15 34 00000000 Cell Proliferation ELISA BrdU Cheml unnnescence 100 10 OO 2014 0409 2014 04 09 10 Sucr ose 10 00000000000000000 BOBO O O DO DO DO DD OD DOLO Anti body DI uti on Sol uti on 100 000000000 O 0000000000000 UL 0000000000 JU 00000 UL U 0 0 0 0 0000000000 0000000000 00000 Vishi ng Buffer 10x U U U U U 0 0 g 0 0 T 000 0000000 0000000000000 00000000 0000 0 CH 16 34 10000000 Cell Proliferation ELI SA BrdU Cheml unnnescence IRR 10 OO 2014 0409
7. es rz 10 A 12 34 00000000 Cell Proliferati on ELISA BrdU Cheml unnnescence 100 10 1 0 2014 0409 10 O 2014 04 09 10000 Fix Denat 0000 00 00000 100000 LEBO OO 7 000 ny kg LEBO I DI 3 450 ny kg 100000 LGGOO OO 20000 ppm 1000 10h 50 00 39 gna 0000 4 000000000000 on TU 20000 0000000000 00000000000000000 H 10 0000000000 EJ 0000000000000 10000 00000000 0 00000000 in vitro 10000 DOR Aws 00 0000 10000 0000 00 TEE KOO ZEE OO se Lr DD 0000000000000 13 34 10000000 Cell Proliferation ELI SA BrdU Cheml unnnescence IDO 10 2014 04 09 2014 04 09 P r3 r3 DO DO DD DO EJ EJ EJ ETE DO DO DD 0000000000000 P DO O DO DD DO
8. 2014 04 09 000000000 0000000000 00000 Substrate Conponent A EEN Z OEI 0 0 0 O 0 ID 0000000 0000000000000 000000000 0000 0000000000 0000000000 00000 Substrate Conponent B 0000 on 0 O 0 U U U O U U U U 0 00 00 00 IL 00 IL 00 IL 00 00 00 IL 00 00 00 IL 00 D 17 34 00000000 Cell Proliferati on ELISA BrdU Cheml unnnescence 100 10 O 2014 04 09 2014 04 09 100000 0000000 gt 2 000 ny kg 00 0000000 00 000000000 0000 000000 L 0 D 1 200 ny kg L 0 1 060 ny kg 10000000 100000 1260 0 5 400 ng kg LO50 O O gt 6 730 ny kg LEBO I 0 0 gt 7 000 ny kg 000000000000 10 00000000 0 00000000000000 00000000000000000 10 000000000 1 0 0000000 EE L3 r3 aa 00000 JUD UD 00000000 IL IL
9. EJ EJ Ki E EJ EJ EJ N 5 L3 C3 r3 OOD EJ EJ EJ EJ 3 N L3 LL Ld ES Ld 1113 DI Ld EJ EJ EJ EJ EJ EJ P264 0 P2701 0 EJ DO n P2710 00 P2800 0 0000 P303 P361 P3330 00000 00000000 0000000 0000 P304 P3400 000000000000 00000000 00000 0000000000 P308 P31311 0 00 000 P370 P378 0000000 00 0 00000000000000 1 0 000 0000 00000000000 IODD IDDU 060 00000000000000 10000 2134 00000000 Cell Proliferation
10. 0000000000000 00 00000000 EEE 00000 0000000000 000 ID 000 100 000 ID 000 r Z m 18 34 10000000 Cell Proliferation ELI SA BrdU Cheml unnnescence IDO 10 10 O 2014 04 09 0 O 2014 04 09 010 000000000 0000 1000000000000000000 0000000000000 100000 00000000 000000 0000000000000000000 0000000000000 00000000000000 00 000000000 0000 1000000000000000000 0000000000000 1000000000000000000 0000000000000 HONOL LI 00000000 100000 000000000 0 000000000 0000 00000000 00000 2 100000 BraU Label ing Reagent 1000x 0000 JOB 0000 00000000000 10000 1000000000 UUTOTOUL 10000000000 HH gt 000 2 00000000 V
11. 7134 10000000 Cell Proliferation ELI SA BrdU Cheml unnnescence IDO 10 10 O 2014 04 09 0 O 2014 04 09 000 1000000 UI EHH 89768 EG100000000 100000000000000000000000000 100000000000000000000000 000 100000000000000000000000000 100000000000000000000000000 1000000000000000000d0U 100000000000 100000000000000000000000000 10000 100000000000000000000000000 1000 0000 10000 qu qu 0 0000000000 00000 000000 0000000000 9 01000000000 BraU Label ng Reagent 1000x 00 10000 10000 10000 100000000 10000 10000 00 UU UL 7 4 00000 00000 00000006 00000000000000 IL 100000 00000000000 1000000 0000000 000 100000 0000 0000 8 34 000
12. 0 000 r3 rpm 0000 0000 000 H D 000000000 1000 0 0000 10 000000 Bottle 3 Anti BRDU POD 0000 10 Sucr ose 000000 0000 1000 000000000000 HH 000000000000000 EJ 29 700 ny kg 14 000 my kg 230 ny JO IL 55 001 ny kg 00000000000 IL EJ 3 613 ny kg 14 34 00000000 Cell Proliferation ELISA BrdU Cheml unnnescence 100 10 0 2014 04 09 2014 04 09 10 0000000000000 00000000000000000
13. 0 0 0 000000000000 10000000000 00000 02000000 on 00000000 0000 10 00000000000000000000 00000 LL 20000000 n000000000000 log Pow 172 0000000 00000 000000 10000 000000000 000 13 000000 0000 JUUDD UDDUUUDOUDUDUDDUUUDUDUDUDDUUUDUDUI 100000000000000000 10000000 0000000808 100000000000000 0 00000000000 ER RE 14 7100000 0000 IATA 0000 1170 25 34 10000000 Cell Proliferation ELI SA BrdU Cheml unnnescence JID 10 2014 04 09 2014 04 09 1000 00000 008140 3 1000 II RENE 3 EEE DA 364 100000000 364 i ugaumualuulutlt Y341 10000 000 I MG 1000 1170 10000 00000 1000 3 1000 dH 000 3 F En FE S En SD 100000 000000 100 MARPCL 73 78 10110018000000000000000000000000000 O 10000 00000 00 15 10000 15 0000 BrdU Label i ng Reagent 1000x 0000 000 000 000 100000000000000000000000000000 2000000 000000000000 O 0000000
14. EJ EJ ZE EJ L4 e L3 DEL 0111 0 0000 00 L3 t L3 C3 C3 34 34
15. 00000 0000000000 1000 0 GSC UI UI 0000 0 000 0000000 100 00 0000000 0000 0 000000 100 00 00 00 1000 0 1000 100000G5100000000000000 Substrate Conponent B 00 00 00 U D D U 0 10000 Q0000Q00000d 10000 000 Uu EE 0000000000 000 0 0 08 60 0 0 0000 0200 1000000 00000 1000000 00000 000000 00000 0000 00000 10 10000000 000 zo BAD BP ETE BEER T EE EH EECHELEN 000000 000 000000 00000000 000000000000 OO BOBO DB ODO ODO 0000000 000000 00000000000 0000000 1000000 00000000 Cell Proliferation ELISA BrdU Cheml unnnescence 100 10 IR 2014 04 09 10 O 2014 04 09 1000000 100000 1000 10000000 00 100000 000000 000 00 1L 0000 BraU Label ng Reagent 1000x 0000 nn 5000 2 00000000 000 000 LEBQ 0 000 8 400 my kg 0600 0 000 9 100 ny kg 000000000000 0000 0 00000000000000000 1000 0 0000 0000 0000 000000000 0000 HH 5000 2 00000000 in vitro 1000 DU in vitro 0000 HU 5000 2 0000 0 EJ EJ EJ P E El El El 0000000000 0000000000 00000
16. 10 000 2014 04 09 10 O 2014 04 09 10000000000 DONDOO 00000000000 OD 0000000000 0000000000 0 00000000000000 100000000000000000000000000 0000000 1000000000000 000000 NNNNA 1 1 1000000000 10000009 10000 100000 100000000000000000000000000 00000 100000000000000000000000000 100000000000000000000000000 JOB 000 DD 0 DO 00000 0000000000 1000000000000000000 EN 000 10000000000 00000000 1000000 100000000 6 100000 1000000000 000000000 00000000000 JO GOOOODOOUOOODOOODOD 10000000000 10000000000000000000U0U DONDOO 0000000000000 00000000 000000000000000 0 00 00 000000000
17. 0 20000000000 000 OD D OO 000000000090 10000 20 34 00000000 Cell Proliferation ELISA BrdU Cheml unnnescence 100 10 2014 04 09 1 2014 04 09 00000000 000 L 000 00 000 0 0 0 023 45 4 nyi 1000 96h LG0 01000000000 000000000 0 7 nay 1000000000000 EG0 1000000 0000 40 38 nyl 100000 1000 48h UUUUDULUUUU 000 0 0 0 0000 0000 0000 0000 0 DD DD DOLO UL 1000000000000 000 0 0 00 0 0 0000 UO 000 0 0 0 00 000 0 0 00 000 0000 000 0 0 0 0 0 ooo 0000 0 0000000 10000 Uu gu 000090 UHUHLHHLH 10000 000000 10000 000000000 100 Bottle 3 Anti BRDU POD 000 0 10 Sucrose Hut LC50 2100 ny 1000 96h 000 0000 gt 1 nyi 0000000000 000 0 0 0 0000 0000 ULHHLHHLHHLHLLULLU o O 0000 000 000 0000 BOBO DO DO DD DDD DOLO UL 21 34 0000
18. 00 00000000 QU 000000 00000000000 000000000 100 Anti body DI uti on Sol uti on 0000 00 22 34 10000000 Cell Proliferation ELI SA BrdU Cheml unnnescence 100 10 J OO 2014 0409 2014 04 09 1000000000 10000000 00000000000 EJ 208000000 00000 0000 1000 0000 1000 0000 0000 0000 1000 000000000 000 V shi ng Buffer 10x 0000 EJ EJ EJ 0000 1000 0000 1000 0000 0000 0000 1000 000000000 O Substrate Conponent A 0000 23 34 00000000 Cell Proliferati on ELISA BrdU Cheml unnnescence
19. 0000 000000 0000000 00000000000 00 13000 0 00000000 1000000000000 EA EET 0000000000000000U0 000000000 0000000000000000 0000000000 000000000000 00 5 34 00000000 Cell Proliferation ELISA BrdU Cheml unnnescence 100 10 OO 2014 04 09 2014 04 09 7 00000000000 000 00000000 100000000000000 10 0000000000 100000000000000 10000000000 8000000 100000000000000000O 1000000000000 UD 1000000000000000 Oda aaa LULU ET E I I 7 100000000 10000000000000000000000O 100000000000000000000000000 10200108 LU Lu 1000 1000 ER 100000000000 1000000 1000000000000 00 1000000 100 10000000000000000000000 100000000000000000000000000 100000000 1000000000000 100000000000000000000000000 10000 8
20. 0000 Cell Proliferation ELISA BrdU Cheml unnnescence 100 10 I 2014 04 09 10 2014 04 09 000 0 0 0 00 BOBO BO DO DD DDD DO DDO 000000000090 00000 000000000000000 000 50 000 00 0 0 0 0 0 0 0 0000 19 nyi UE 96h 1000000000000 50 0 00 00 0 0 0 0 0 023232 nyi 100000 1000 96h 10000000 1600000000000000000 00 0 2 nyi Pu EH 96 4 00 00 0 00 0000 0000 000 000 0000 BOBO DO DO DD DD DD DD DOLO 000 0 0 0 0 0 BEBE BO BO DD DDD DO OD DO 000000000090 000090 0000000 00 000000000000000 But DO dd BO DDD DDD DO 00000 42 Sucrose 000000000000 log Pow 3 67 0000000 10000 0000040 10000 0 000000000000000 10000000000 POB 000000000000 000000000000 dOdg dd dog gon 0000
21. 00000 00000000000 g 20000 T 0000 DO EJ OE DO 29 34 00000000 Cell Proliferation ELISA BrdU Cheml unnnescence 100 10 OO 2014 0409 2014 04 09 000 100 00000000000 00 IL DO DD 00000000000 Anti body DI uti on Sol uti on 0000 000 30 34 00000000 Cell Proliferation ELISA BrdU Cheml unnnescence 100 10 D 2014 04 09 2014 04 09 000000 IL DD O DD DD O O L3 Lr DD 00000000000 O O 00 UL Lr 000000000000 V shi ng Buffer 10x 00 00 00 0 NEEE ET RAR 1000 1000 10000
22. 00000 Cell Proliferation ELISA BrdU Cheml unnnescence IOO 10 O 2014 04 09 JO 0 2014 04 09 Fi x Denat L 00 IL 100000 pH 13 7 100000 00000 100000000000 00000 000 18 C 1000000 10000 1000000 10000 100000 10000 1000 00000 0000 100000G610000000000000 Bottle 3 Anti BRDU POD 100000000000 U UD IL pH 7 3 7500000000 000 1000 1000000000 00000000 10 0 000 1 U D LU UU 10 00 00 0 0 00 0 8 0 0 0 0 0 0 0 0 0 0 100 6010 Anti body DI uti on Sol uti on UD UL 00 L U pH 7 0 7 4 9 34 00000000 Cell Proliferation ELISA BrdU Cheml unnnescence
23. 000000 100 000000000000 100 000000000U00000000U0000 100 26 34 00000000 Cell Proliferati on ELISA BrdU Cheml unnnescence 100 10 10 0 2014 04 09 100 2014 04 09 DL ELE BEL E 0 0 EE E B EE EE EF 7E B 7E 7E 7U EE 7I U C 100 I HHHHHHHHHHULLULLU 000 1000 E 0 0 EE EE EE EE EE EE 7T EE EI L 000 100000000000 000 1000000 00 000 0 0 00 0000 OO 100 100000000 100 1000000000 1000 0090 000 0000000090 000 0000000000000 000 0000000 000 000000 000 0000 0 10000000000 000 000 10000000000000 0000000000000000 10000 Fix Denat 0000 000 000 000000 400000 000 00000000000000000000000U00U0U000u0 0000000 100000000000 27 34 00000000 Cell Proliferation ELI SA BrdU Cheml unnnescence 000 10 2014 04 09 OOO 2014 04 09 100000000000000000000 10000000000000 0 000000000000000 00000000000 000000000
24. 00000000 Cell Proliferation ELI SA BrdU Cheml unnnescence 000 10 OOO 2014 04 09 000 2014 04 09 1 000000000 000000 0000 00000 00000000 3 or 3 or L3 L3 L3 or 3 L3 L3 E3 E 000000000000 000000 Cell Proliferation ELI SA BrdU Cheml unnnescence 11669915001 000000261 1050014 03 5443 5128 03 5443 5299 000000 261 00000000000 0354437081 IL 0120 868 555 TD 03 5443 5287 AS 0000 0 0000000000001 2 00000000 GBI O 00000 002 00000000 00018 0000 BETA 00000000 0000 00300000 0000 00000000 0000 00100 00000000 0000 00200000 061 00 0 000000000 10000 uu 1 34 00000000 Cell Proliferati on ELISA BrdU Cheml unnnescence 100 10 D 2014 04 09 O 2014 04 09 0000000 2500000 1335 1336 1000000 100 1000000 0000000000 000 cb EJ OOO 000 DO
25. 10000000000 BraU Label i ng Reagent 1000x 00000000000 0000 I HHHHHHHHEHEHHHHULHU Fix Denat 00000000000 0000 0 1 900 mg m3 TVA 1 000ppm OSHA Z 1 6 34 00000000 Cell Proliferation ELI SA BrdU Cheml unnnescence 100 10 100 2014 04 09 2014 04 09 1 900 mg m3 5 as 1 900 mg m3 ama oma c Ced o eror 10 CC 2mgm3 JACGH O 2mg m3 NIOSHREL TWA 2mgm3 OSHAZ 1 C 2mg m3 OSHAPO Bottle 3 Anti BRDU POD 00000000000 0000 0 EL ERE sucrose 57 50 1 a 15 mg m3 ACGIH TWA 5 mg m3 NIOSH REL Respirable TWA total NIOSH REL TWA total 15 mg m3 OSHA Z 1 dust 5 mg m3 OSHA Z 1 respirable fraction kJ Total 15 k 5 7 7 PO 7 dust fraction Anti body D uti on Sol uti on 00000000000 0000 10000000 0 00 00 0220 V shi ng Buffer 10x 00000000000 0000 10000000 0 00 0 0000 Substrate Conponent A 00000000000 0000 0O0QADo0 0 000000000000 Substrate Conponent B 00000000000 0000 0 00 0 0 000000000000
26. ELI SA BrdU Cheml unnnescence DOD 10 DOO 2014 04 09 2014 04 09 3 00000000 BrdU Label i ng Reagent 1000x 10000000000 BUD 000000 5000 2 00000000 59 14 3 2 0 1 1 CASI EJ BGB AEA P HER LER E TAA 7e Bottle 3 Anti BRDU PCD 10000000000 000 000000 000 0000000 YA z aa pa Anti body DI uti on Sol uti on 10000000000 000 000000 00000000000000000000000096000011000000000 96000000000000000000000000000000000 20057 100 10000000000 000 000000 3 34 00000000 Cell Proliferation ELI SA BrdU Cheml unnnescence 000 10 2014 04 09 OOO 2014 04 09 0000000000000000000000000G6000000000000000 c5000000000000000000000000000000000 Substr
27. ERE Brot HEP EE EE U 1000000000000000 000000000000000 00000000000 0000000000000 00000000 19 34 10000000 Cell Proliferation ELI SA BrdU Cheml unnnescence 100 10 10 H 2014 04 09 10 O 2014 04 09 000000000090 10000 0000000 00 00 0 00000 00 00 0 0000000 10000 000000 10000 000000000 000 Fix Denat 0000 OD 00 0 0 0 00 000 10000000 DL ELE B EE E B EE EE B DE 7E DB 7E 7I U EC 000000000090 10000 00 00000 000 1 60 00000000000 07000000000 8 000 ng 1000 48h LS0 000000000000 0 0000 7 100 ny 10 2 0 0000 00 0 203 0000 0 0 0 0 ooo EC50 0 B E D D DD 2 5 400 ny 100000 1000 48h 10000000 0 0 00 0 00 0 00 0 0 0 0 0 0 0 5 000 nyl lH Ed 10000000000 E Pseudononas putida 0000000 00 6 500 ng 1000 16 000 0 0 0000 1000 0 0
28. ate Conponent A 10000000000 IL 000000 0000000000000000000000000G6000000000000000 c5000000000000000000000000000000000 Substrate Conponent B 00000000000 000 000000 000 El EJ El Se e 4 0000 00000000000 0000000 10000000000000d00 IRA 10000000000 100000000000000000O 10000000000 10 0000000000000 0000000o0UU 100000000000000000O 100000000000000000000000000 0 0000000000000 10000000000000000 100000000000000 100000000000000000000000000 1000000000000000 In 10000000 000000000000000 000000 000000000 0000000 1000000 4 34 00000000 Cell Proliferation ELI SA BrdU Cheml unnnescence 100
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