Home

T6-RD1

1. 2 3 4 5 6 pk 2 8 C 2 20 25 C
2. gt PBS PBS 2 8 C NBS 29 BSA 0 196 0 19 gt b 3 x 10 mm 1 5 L 5L 195L PBS 1 25C 500 xg 1 700rpm 5 2 ER 3
3. T T Harvard Medical School Dr S F Schlossman Coulter Immunology T B 3
4. 10 11 T6 RD1 12 13 CD1a T6 RD1 14 15 16
5. 6 7 Fc 10 15 1mg mL 8 9
6. 85 1 1 100 L 3 10 x 103 mm 10x10 mm 3x10 mm PBS 2025 C 6 gt PBS a gt 10 x 10 mm PBS PBS 2 8 C NBS 2 10 20 x103 2 BSA 0 19 20 30 x103 3 0 19 30 40 x10 5
7. T6 CD1a CD1a 80 T non T CD1a B CD20 85 100 T 3 9 CV 59 1 0 196
8. 6 2 90 3 859 4 Ficol Paque Ficoll Paque 5 5 Ficoll Paque
9. PBS 25 1mL 30 2 250 gL 7 PBS 2mL 8 400 lt 450 xg 5 9 7 8 10 0 5 1mL PBS EPICS 11 2 Ficol Paque 1 12mm x75mm 2 Ficol Paque 1 x 10 1x10 mm 1mL 3 2 8 C 400 450 x g 4
10. 859 4 Propidium lodide Calbiochem 53705 0 01mg mL 0 05mg mL 5 Acridine orange Baker A366 3 0 005mg mL 6 Ficoll Paque Pharmacia 17 0840 03 b 12 25 000 1x 10 L 25 L 13 Propidium lodide 0 01mgimL 10L 14 30 Acridine orange 0 005mg mL 10L EDTA 3 15 16 100
11. T B Stage II CD1a T6 CD1a T CD1a T CD1a T T CD1a SCID T CD1a Mycosis fungoides
12. 4 1 5 200wL MSlgG1 RD1 200L 6 2 lt 8C 30 7 2 8 C 400 450 x g 5 8 3 4 9 PBS 200 L 2 8 C 400 lt 450 x g 5 10 3 4 11 PBS 200L EPICS 1 3 4 2
13. 6603185 2009 11 2007 2 20200EZY00023000 877429 T6 RD1 4 RD1 Phycoerythrin PE 1 0 5 2 5 g IO w T6 CD1a 49kD CD1a 80 T non T CD1a
14. Stage II CD1a T CD1a T T non T CD1a T6 SFCl19Thy1A8 CD1a BALB cJ NS 1 AG4 lg IgG1H zL T6 RD1 RD1 Phycoerythrin PE RD1 0 5 1 5 486 580nm 568 lt 590nm 1 0 5mL 0 296 0 01M 0 15M
15. Ficoll Paque 3 RD1 PE Log 1 CD1a CYTO TROL 6604248 Fc Fc MSlgG1 RD1 1 29 2
16. 0 196 T T6 CD1a 1 4 CD1a 488nm RD1 4 FS 90 SS 1 EDTA
17. 4 LH 700 1 PBS PBS 6603369 1 500mL pH 7 2 0 2 0 196 2 1 2 1 6603152 300 PBS 24mL 1mL 9 25 2 1L 8 26g 1 0g EDTA4Na 2Na 37mg pH 7 2 7 4 1 1 2
18. PBS PBS 0 1 40 60 x103 6 2 8 C 60 100 x103 10 100 200 x 103 20 lt gt 1 1 2 4 2 100L 3 200L MSlgG1 RD1 200L 4 45 5 PBS 2 3mL 400 450 x g 5 6 a b a 2mL 5 15 b
19. 6 PBS 7 2 lt 8 400 lt 450 x g 4 8 PBS 9 2 lt 8 400 lt 450 x g 3 10 PBS 1x10 mm 1 x 10 mL 11 a b 90 a 12 1x10 1x103 mm 1mL 13 2 8 PBS 2 8 C 400 450 x g 4 14 0 05mg mL Propidium lodide 3 1 15 2 8C PBS 2 8 400 lt 450 x9 4 16 15 17
20. 3 T6 RD1 6603185 100 0 5mL 1 Reinherz EL Kung PC Goldstein G Levey R and Schlossman SF 1980 Discrete stages of human intrathymic differentiation Analysis of normal thymocyies and leukemfc lymphoblasts of T lineage Proc Natl Acad Sci USA 77 1588 2 Reinherz EL and Schlossman SF 1980 The differentiation and function of human T Ilymphocytes Cell 821 3 Reinherz EL and Schlossman SF 1981 Derivation of human T cell leukemias Cancer res 41 4767 4 Bharn AK Reinherz EL Poppema S McCluskey R and Schfossman SF 1980 Location of T cell and MHC antigens in the human thymus J Exp Med 1 52 772 5 Boumsell L Bernard A Reinherz EL Nadlar LM Ritz J Coppin H Richard H Dubertret L Dagos J Lermil J Flandrin G Dausset J and Schlossman SF 1981 Surface antigens on malignant Sezary and T CLL cells corresponds to those mature T cells Blood 57 52 6 Reinherz EL Schlossman SF and Rose Fs 1980 Human immunodeficfency states resulting from disorders of T cell maturation and regulation
21. 30 2 10 15 20 30 3 MslgG1 RD1 6602884 100 0 5mL 5 10x10 mm 1 100L 2 Ficoll Paque 1 4mL PBS 2 Ficol Paque 4mL 1 3 2 lt 8 400 450 xg 30 4 Ficol Paque 5 2 8C PBS 2 8 C 400 450 Xx g 8
22. 4 200 L MSlgG1 RD1 200 L 5 2 8 C 30 6 PBS 1mL 2 8 C 400 450 x g 4 7 5 2 8 0 5 1mL PBS 9 EPICS 3 Ficoll Paque 1 1 x 10 200L Ficoll Paque U 96 200pL 2 2 8 C 400 450 x g 5 3
23. AC Hussey RE Fitzgerald KA Daley JF Levine H Rose RS and Schlossman SF 1982 Reconstitution after transplantation with T lymphocyte depleted HLA haplotype mismatched bone marrow for severe combined immunodeficiency Proc Natl Acad Sci USA 79 6047 14 Umiel T Daley JF Bhan AK Levey R Schlossman SF and Reinherz EL 1980 Acquisition of immune competence by a subset of human cortical thymocyies expressing mature T cell antigens J Immunol 129 1054 15 Koepke JA and Landay AL 1989 Precision and accuracy of absolute lymphocyte counts Clin Immunol Immunopathol 52 19 27 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC Ss COULTER 6603185_0911
24. In Primary Immunodeficiencies Seligmann M and Hitzig WH eds ElsevierNorth Holland Biomedical Press Amsterdam Dp 109 7 Bernard A Boumsell L Reinherz EL Nadlar LM Ritz J Coppin H Richard H Valensi F Dausset J Flandrin G Lemerle J and Schlossman SF 1 981 Cell surface characterization of malignant T cells from lymphoblastic lymphoma using monoclonal antibpodies Evidence for phenotypic differences between malignant T cells from patients with acute lymphoblastic leukemia and lyrnphoblastic lyrnphoma Blood 57 1105 8 VTerhorst C an Agthoven A LeClair K Snow P Reiherz EL and Schlossman SF 1981 Biochemical studies of the human thymic differentiation antigens T6 T9 and 11 Cell 23 771 9 Nadler LM Ritz J Griffin JD Todd RF Reinherz EL and Schlossman SF 1981 Diagnosis and treatment of human leukemias and Ilymphomas utilizing monoclonal antibodies In Progress In Hematology Brown EB ed vol Xll pp 187 10 McMichael AJ Pilch J Galfey G Mason DY Fabre JW and Milstein C 1979 A human thymocyte antigen defined by hybrid myeloma monoclonal antibodies Eur J Immunol 9 205 4 4 11 Reinherz EL Cooper MD Schlossman SF and Rosen FS 1979 Abnormalities of T cell maturation and regulation in human beings with immunodeficiency J Clin Invest 68 699 12 Reinherz EL and Rosen FS 1 981 New concepts of immunodeficiency Am J Med 71 511 13 Reinherz EL Geha R Rappeport JM Wilson M Penta

T6-RD1

Contents

Download Pdf Manuals

Related Search

Related Contents