T11-RD1
Contents
1. No Wash No Wash Q PREP TQ Prep Q PREP TQ Prep T DiGeorge T T 19 65 T112. 3 4 3 4 5 7 1 2 8 C
3. 1 2 100L 3 10xL MSlgG1 RD1 10L 4 KGL Bint 45 5 PBS 2 3mL 400 lt 450 x g 5 LET 6 7 PBS 25 1mL 30 2 8 250 kL MA HHLET 9 PBS 2mL 10 400 450 x g 5 11
4. 9 MslgG1 RD1 10 11 CD2 TT NK CD2 T CD3 12 1 10L 1 1
5. Pan T Pan B T B pan T CD T11 T
6. T CD2 488nm Dich FS 90 SS 1 2 3 EDTA
7. LFA 2 BH 95 T NK CD2 T CD2 B SFCI3Pt2H9 T11 T BALB c NS 1 AG4 lg lgG1 H k L T11 RD1 RD1 Phycoerythrin PE RD1 0 5 1 5 486 580nm 568590nm 1 0 5mL BSA 0 2 0 01M NaCl 0 15M NaN 0 1 E T
8. EDTA 1 100 1 1 2mL 3 10 x 10 A mm 10 x 10 mm 3x 10 mm Q PREP PBS 20 25 6 A gt 10 x 10 mm 10 20 x 10 2 20 30 x 10 3 30 40 x 10 5 40 60 x 10 6 60 100 x 10
9. 6 4 90 5 6 7
10. Q Prep 1 12mm x 75mm 2 100L 3 10uxL MSlgG1 RD1 10 L 4 10 5 TQ Prep Q Prep 0 5mL 6 2 4 2 2 2 lt 8 24 7 B
11. 6603849 2009 11 2007 2 20200EZY00028000 877449 T T11 RD1 2 4 lt _ wm RD1 Phycoerythrin PE 1 T11 RD1 0 1 0 6 ku g T11 CD2 50kD CD2 E T pan T
12. 2 3 T11 RD1 6603849 50 0 5mL Bernard A Boumsell L Dausset J Milstein C and Schlossman SF eds 1984 Leukocyte Typing New York Springer Verlag p 26 28 41 42 196 201 215 216 2 McMichael A J ed 1987 Leukocyte Typing Ill Oxford Oxford University Press p 38 40 42 106 107 116 199 302 306 308 315 475 3 Reinherz EL Haynes BF Nadler LM and Bernstein ID 1986 Leukocyte Typing Il New York Springer Verlag Vol 1 p 6 16 364 365 4 Reinherz EL and Schlossman SF 1980 The differentiation and function of human T lymphocytes Cell 821 827 5 Nadler LM Anderson KC Marti G Bates M Park E Daley JF and Scholossman SF 1983 B4 a human B lymphocyte associated antigen expressed on normal mitogen activated and malignant B lymphocytes J Immunol 131 244 250 6 Reinherz EL and Schlossman SF 1981 The characterization and function of human immunoregulatory T lymphocyte subsets Immunol Today 2 69 75 7 Koepke JA and Landay AL 1989 Precision and accuracy of absolute lymphocyte counts Clin Immunol Immunopathol 52 19 27 m o
13. 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC Wa COULTER 6603849_0911 4 4
14. x 10 6604248 6607077 Fc MSlgG1 RD1 1 lt 29 29 ERA MOU SERIE T B NK 3
15. 60 929 TH CD2 B CD20 85 100 95 T NK B 3 10 T11 9 CV T11 RD1 n SD CV 1 10 218 0 62 2 9 2 10 782 0 39 0 5 3 10 885 0 40 0 4 1 0 1
16. 7546999 300 TQ Prep Multi Q Prep 7546946 100 Q Prep 2 6603152 300 1mL PBS 24mL 9 259 3 2 PBS PBS 6603369 1 500mL pH 7 2402 3 MslgG1 RD1 T11 RD1 FA 6604112 50 0 5mL
17. 10 100 200 x 10 20 B 3 x 10 mm 1 25C 500 x g 5 2 COR FAT 3 4 LH700 5 3 10 x 10 mm 1 1004 L 1 TQ Prep Q Prep O A Q B C 2 A
18. 12 9 11 13 PBS 500L 14 1 2 FS SS 3 FS 3 RD1 PE EH Log 1 1 Q Prep CD2 Flow Count 7547053 mm nm x
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