NH IMMUNOCHROMATO O26
Contents
1.2. 3 026 2 6 1 X104 10 CFU mL 1 NH 026 D i 1 MM ve penne nee
3. 121 20 0 1 1 1 o 1 RTA 2 4 2 8 12 NH O26 20 TARA 1 168 179 2004 2 0157 026 18 11 2 1102004 5
4. 2 3 O26 026
5. 1 026
6. 026 026 4 ae SPs S285 3 y 3 15
7. D RN rel peels ae MM 3 Miek 25g mEC 225mL 1 1 42 18 24 1 mEC mEC OO 4mg mL mEC 1 000mL 5mL 20mg L 2 mEC TSB
8. 300 2646 3 3 029 847 7825 FAX 029 847 7824 URL http www rdc nipponham co jp E Gat 999400000 20 1 1 NH 026 5 Vero toxin VT Hemolytic Uremic Syndrome HUS CS svelte eae
9. O26 O157 18 11 0157 026 1102004 O26 gt 1 1 2 3 1 2 1 AS FARI peter ies 5 TARX4 026 ee 0 25 ug B PRHE eee 1 026
10. TSB mTSB TSB CTV mTSB VCC 7 m 1 026 1X105CFU mL 4 ECD ITHHEL CCE EU O 5mL 121 20 ii 2 026 3 1 2
11. 0 17g 0 25 ug 1 026 1 026 1 f a e at 6 30mm 38mm m mo 2 O26 2 O26 1 O26 3
12. 30mm 38mm D Rta ME Dpt S A Ji BSH EA O ITIL 2 4 Bay vy HY gt Yury Paes p eat 200g ER 300 500m O OE JES 0 2 0 3mm 25g
13. 100zL 4 150 L PF RR D ni ia ID 15 Esheri chia coli Q57 ATG 25922 ATCC11775 ATCC700728 R MD5091061 Esheri chia coli Q11 RI MDD509829 R MD5091865 Esheri chia coli OL ATGC11775 Esheri chi a coli 5 R MDD509829 100L G trobacter Freund i ATCC8090 ER Enterobacter aerogenes ATCC13048 PAT PEE SOUL EAE Frterobacter cloacae ATCC13047 ATCC49141 Enterobacter sakazaki i ATCS1329 Esheri chia hernami i J M473 K ebsi el la pneunoni ae ATC4352 1 es7 ella
14. gt COSMO BIO CO LTD Inspiration for Life Science Reagent for food testing January 2008 1 edition NH IMMUNOCHROMATO 026 Please read this manual carefully before using the kit Instruction Manual Product number 999400000 Introduction Food poisoning by Diarrheagenic Escherichia coli is an infectious food poisoning that is caused by the growth of E coli taken into the intestinal tract though the ingestion of food These Diarrheagenic E coli can be broadly classified into five types Among these Enterohemorrhagic E coli EHEC causes hemorrhagic colitis through the action of the verotoxin that it produces it can also cause hemolytic uremic syndrome and encephalopathy which can be fatal Many cases of mass food poisoning by EHEC have occurred so EHEC is viewed as the most dangerous among Diarrheagenic E coli from the standpoint of food hygiene The recent increase in the serotype O26 isolated from patients with diarrheagenic coli infection has made it as the second commonest serotype after O157 The Ministry of Health Labour and Welfare of Japan therefore issued a notification of the Test method of diarrheagenic E coli 0157 and 026 Notification No 1102004 issued by Inspection and Safety Division in November 2006 This product is a kit for detecting E coli O26 in foods by immunochromatography Tests can be conducted rapidly and simply by means of the kit Product Features 1 The simple one step ope
15. manufacturers or distributors that any reagents including culture media required for preparation of test solutions and any instruments that are used are suitable for the purpose This instruction manual is intended as a guideline for those in charge of testing Verify your own operating procedures for the kit and the appropriateness of its use for each particular food Product specifications may be changed without notice 2 Precautions Regarding Risk Prevention a 2 3 4 Even minute amounts of E coli 026 which the kit is designed to detect could cause infection For this reason and because there is a possibility of infection by microorganisms than other than coli O26 exercise full precautions in conducting tests by wearing protective gloves and safety glasses Tests should be performed only where appropriate equipment and facilities are available Follow standard microorganism testing procedures under the guidance of responsible supervisors If you accidentally get any sample solution in your eyes or mouth adopt emergency measures such as immediately washing away the solution with tap water and then seek medical attention If you feel unwell after performing a test with the kit obtain immediate treatment from a physician 3 Precautions Regarding Disposal of Waste Materials a 2 Note that surplus test solutions and used test plates culture media and test samples could carry contagious microorganisms The
16. 6 1 These complexes move to the expanded section by capillary attraction D X and are trapped by the anti E coli O26 antibody 3 that is fixed in the test line appearance position This results in the appearance of a reddish purple line of gold colloid This reddish purple line can be detected by visual inspection and 2 3 4 used to judge the presence or absence of E coli 026 in the test solution The excess gold labeled antibodies regardless of the presence or absence of E coli 026 in the test solution travel further through the expanded section and are trapped by the anti goat immunoglobulin rabbit antibody 4 fixed at the control line appearance position where they form a second reddish purple line The presence of this line indicates that the test solution has reached the expanded section COSMO BIO CO LTD Inspiration for Life Science Preparation of the sample Solution The method for preparing a sample solution is described in accordance with the Japanese official testing method 1 Required Equipment and Instruments Stomacher bag preferably with a filter stomacher incubator autoclave culture medium etc 2 Preparation of Test Samples 1 Take a test sample of more than 200g of the food under test In cases where surface contamination is suspected the sample is taken by scraping off 300 500cm of the surface to a thickness of 0 2 0 3mm 2 Chop and mix the whole sample collected Weigh 25g of the sample into the stomach
17. at PE p 8 E 2 Negative the test line appearance position It is likely that there is something abnormal in the development of the sample solution in such cases LA I TL Notel Ensure that confirmatory tests in accordance with the official test method or other methods are performed on specimens that are tested positive by the kit The proliferated and cultivated samples 3 Retest used for testing with the kit can be used in confirmatory tests by the official or other test method 1 Sensitivity Test The results of tests conducted in accordance with instructions for the preparation of the sample solution and the operating procedures for testing Performance described in this manual will be positive when the concentration of E coli 026 organisms is more than 1 x 10 CFU mL 2 Repeatability Tests When positive and negative test solutions of E coli O26 were simultaneously tested three times each all positive test solutions exhibited positive results and all negative test solutions showed negative results 3 Minimum Detection Sensitivity The results of testing standard three strains of E coli O26 and four strains of isolated bacteria confirmed that the minimum detection sensitivity is between 1 x 104 and 1 x 10 CFU mL Notel The minimum detection sensitivity of this kit could vary depending on the effects of the components of the test solution 4 Cross reactivity 1 Cross reactivity with the following bacte
18. er bag and use this as the test specimen 3 4 3 Sample enrichment 1 Add 225 mL of mEC broth with novobiocin to the 25g specimen in tomacher bag and homogenize with a stomacher for 1 minute 2 Incubate the specimen in the stomacher bag at 42 C for 18 24 hours Notel When commercial mEC culture medium that does not contain novobiocin is used sterilize the medium by autoclaving and cool it Then sterilize a 4 mg mL solution of novobiocin by filtration and add 5 mL of the solution to 1 L of mEC culture medium to give a final concentration of 20 mg L Note2 Instead of the mEC broth with novobiocin it is possible to use tryptosoya broth TSB or growth and proliferation retardant added TSB mTSB TSB CTV or mTSB VCC selective enrichment broth 4 Sterilization 1 Remove the stomacher bag from the incubator after 18 24 hours Gently mix the contents of the stomacher bag using a side to side motion taking care not to splash it 2 Transfer about 5 mL of the culture solution into a glass test tube by using a sterilized pipette and sterilize the solution in an autoclave for 20 minutes at 121 C 3 Remove the tube from autoclave and allow the tube to cool to room temperature to prepare the sample solution Notel The kit can also detect viable E coli O26 but tests with a sterilized culture solution are recommended to ensure the safety of the operator Note2 Because the remainder of the culture solution might be required for use in c
19. luminum pouch in a refrigerator Note3 Be careful not to scratch the test sample drop section or expanded section and do not touch them with your hands When handling the test plate make sure that you hold the absorbent pad Note4 Make sure that you use a sterilized pipette or chip to drop or dispense the sample solution Change the pipette or chip for every test solution NoteS Make sure that the 150 uL portion of test solution does not overflow the test plate when dropping it If necessary drop the solution in two or more portions Note6 To prevent infection of the operator it is recommended that testing be performed with the test plate A wrap should be placed under the plate when dropping the test solution COSMO BIO CoO LTD Inspiration for Life Science 2 Judgment of the Test Results Test line appearance Control line appearance 1 The test results is judged as positive when a reddish purple line is position 30 mm from position 38 mm from observed at the test line appearance position and at the control line the test sample drop the test sample drop appearance position 15 minutes after the start of the test section section 2 Judge the test results as negative when no reddish purple line is 1 Positive N observed at the test line appearance position but a line is observed at 3 Retest in cases where no reddish purple line is observed at the control appearance position regardless of the presence or absence of a line
20. onfirmatory tests following those conducted with the kit do not sterilize it and retain it until all the tests have been completed Operating Procedures for Testing 1 NH Immunochromato O26 Test Procedures 1 Bring the test plates contained in the aluminum pouch to room temperature and remove from the pouch as necessary immediately before use 2 With an oil based marker pen write the name of the test sample or the number of the subject under test on the absorbent pad of the test plate removed from the bag 3 Place the test plate carefully on the flat stand and drop a 100 nL portion of the test solution onto the test sample drop section see the figure on the left Otherwise dispense a 150 uL portion of the test solution into a test tube and attach the test plate to the test tube so that the test sample drop section of the test plate is immersed in the test solution see the figure on the right 4 Allow the test plate to stand undisturbed for 15 minutes and then visually judge the presence or absence of E coli O26 in the solution Drop a 100 uL portion of the test solution Absorbent pad Dispense a 150 uL portion of the test solution Notel Do not remove the test plate from the aluminum pouch until it has returned to room temperature otherwise incorrect test results may be obtained as a result of moisture absorption Note2 Keep test plates that are not in use in a vinyl pouch containing desiccants and store this in an a
21. oxytoca ATCC8724 m 2 Psudonanas aeruginosa ATCC9027 a 3 Proteus vulgaris ATCC6380 oe l i l Serratia li qufad as ATC27592 4 i ae OE ATG8100 5 100 xL 2 0 0 6 2 3
22. ration of the kit 2 The test gives rapid results 3 There is no need for special test equipment Kit contents 1 Components 2 Ingredients and Quantity A Test plate 5 test x 4 packs A Anti E coli 026 polyclonal antibody rabbit 0 25 ug B Instruction manual 1 sheet B Anti E coli 026 polyclonal antibody labeled with colloidal gold rabbit 0 17 pg C Anti rabbit immunoglobulin polyclonal antibody goat 0 25 ug Application 1 Detection of E coli 026 in Foods Note 1 This kit is intended to specifically detect E coli O26 and therefore cannot detect the presence or absence of verotoxin prodution Illustration of Test plate and the Principle of assay 1 Illustration of Test plate a Sample solution drop section e f g Be careful not to touch this sectoin with your finger b Reagent containing section c Detecting section Be careful not to scratch this section and touch this section with your finger d Absorbent pad e Measurement items listing position f Test line appearance position a b c d Approx 30mm from the _ sample solution drop section g Control line appearance position Approx 38mm from the sample solution drop section 2 Principle of assay When a sample solution is dropped onto the test sample drop section of the test plate the gold colloid labeled anti E coli O26 polycolonal antibody 2 in 1 amp the test sample containing section dissolves and forms complexes with E coli J 02
23. refore make sure that waste materials are subject to appropriate sterilization for example by autoclave treatment for 20 minutes at 121 C or immersion of the materials in a sodium chlorite solution for more than 1 hour Discard the kits test samples and surplus test solutions in strict compliance with your local waste disposal regulations and with full consideration of environmental sanitation Storage Method and Use By Date 1 Storage method Refrigerate at 2 8 C and shade from the light Avoid freezing 2 Use by date 12 months from the date of manufacture Packaging Unit NH Immunochromato 026 20 tests 5 test x 4 References 1 Ministry of Health Labor and Welfare Microorganism Section of Guide to Food Hygiene the Japan Food Hygiene Association pp 2 168 179 2004 Ministry of Health Labour and Welfare Test Method for Diarrheagenic E coli 0157 and 026 Notification No 1102004 November 2 2006 issued by Inspection and Safety Division Manufacturer R amp D Center Nippon Meat Packers Inc 3 3 Midorigahara Tsukuba Ibaraki 300 2646 Japan Phone 81 29 847 7825 Fax 81 29 847 7824 URL http www rdc nipponham co jp Distributor gt COSMO BIO CO LTD Inspiration for Life Science TOYO 2CHOME KOTO KU TOKYO 135 0016 JAPAN http www cosmobio co jp e mail export cosmobio co jp Phone 81 3 5632 9617 FAX 81 3 5632 9618
24. rial strains has not been observed Strains ID of standard strains Results Esherichia coli 0157 ATCC25922 ATCC11775 ATCC700728 RIMD05091061 Esherichia coli O111 RIMD0509829 RIMD05091865 Esherichia coli O1 ATCC11775 Esherichia coli 025 RIMD0509301 Citrobacter freundii ATCC8090 Enterobacter aerogenes ATCC13048 Enterobacter cloacae ATCC13047 ATCC49141 Enterobacter sakazakii ATCCS1329 Esherichia hermannii JCM1473 Klebsiella pneumoniae ATCC4352 Klebsiella oxytoca ATCC8724 Psudomonas aeruginosa ATCC9027 Proteus vulgaris ATCC6380 Serratia liquefaciens ATCC27592 Serratia marcescens ATCC8100 Serratia ororifera ATCC33077 gt COSMO BIO Co LTD Inspiration for Life Science Precautions in Using the Kit 1 Precautions in Handling the Kit a 2 3 4 5 6 7 Read the instruction manual carefully before use Use the kit in accordance with the test method described in this manual Do not use a kit whose use by date has passed The expiry date is indicated on the label on the aluminum pouch The kit is a reagent designed to detect E coli 026 in food It is not to be used for clinical diagnosis Tests may give false positive results as a result the effects of the ingredients present in the specimen Positive test results from the kit should be confirmed by using the official test method or other procedures Confirm with their
Download Pdf Manuals
Related Search