TaKaRa 腸管系病原細菌遺伝子検出キット
Contents
1. 3 1 2 32. PCR PCR PCR PCR Manual PCR dH2O PCR VT Positive Control ipaH Positive Control invA Pos
3. 121 20 http wwwr takara bio coJp 3 RR139A V 1 VI Reaction Mix Fast 100 X SYBR Green
4. 200 20 10 ul 95 5 PCR Thermal Cycler Dice Real Time System Ill TP950 TP970 TP980 Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 CFX96 Touch Real Time PCR Detection System BIO RAD StepOnePlus Rea Time PCR System Life Technologies IV 1 2 3
5. Tm PCR Tm Tm Tm 7295 sis 828 848 646 6e 721 74 1 Thermal Cycler Dice Real Time System PCR PCR Tm gt 12000 10000 1 s 8000 2 eooo 3 1 VT Positive Control 8 a000 2 invA Positive Control 2000 3 ipaH Positive Control 0 80 82 Bs amp 3 90 70 72 4 7 3 Tercersmure 78 88 73 gt 32000 10000 3 6000 4000 gt 3 2000 gt 2000
6. 3 3 Thermal Cycler Dice Real Time System I Speed Fast Normal Fast lt gt 94 30 lt PCR 45 gt 90 1 64 9 SYBR GreenD 68 90 gt Thermal Cycler Dice Real Time System ite Speed Fast lt gt 94 30 lt PCR 45 gt 90 1 64C 1 68 90 gt 64 1 1 CFX96 Touch Real Time PCR Detection System StepOnePlus Real Time PCR System lt gt 94 30 lt PCR 45 gt 90 1 64 C 10 SYBR Green 68 90 gt http www takara bio co jp 5 RR139A 3 Tm
7. gt 1 mF VT 7V4 a IC IC IC PCR DNA 77 UIC VT VT invA ijpaH Tm Tm http wwwr takara bio coJp 7 RR139A VII VIlI Thermal Cycler Dice Real Time System Ill TP950 TP970 TP980 Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 TP950 0 1 ml 8 strip tube individual Flat Caps NJ902 0 1 ml 8 strip tube amp cap Set NJ903 TP900 TP700 0 2 ml 8 strip tube individual Flat Caps NJ600 0 2 m
8. 0 2 1 7 ra n9 82 71 0 Teroeratrw 73 78 88 http www takara bio coJp 6 RR139A gt PCR VT Positive Control invA Positive Control ipaH Positive Control PCR VT MM pg gt EHEC VT MM pg IC lt
9. 828 948 pa 84 6 86 6 PCR 72 1 74 1C Thermal Cycler Dice Real Time System TP900 TP960 PCR PCR Tm 200 TaKaRa PCR RR138A 1 1 Reaction Mix Fast 2 2 1 25 ml Xx 2 2 100 xX SYBR Green 3 50 ul 3 Primer Mix VT ipaH invA 5 X 1 ml 4 dH20 1 ml X 2 TaKaRa Positive Control 6993A 1 6 ositive Contro 1 VTPositive Control 4 50 ul ipaH Positive Contro 2 ipaH Positive C 4 50 wl 3 invAPositive Control 4 50 ul 1 2 dNTP Mixture Mg2 3 4 ll 20 http www takara bio coJp 2 RR139A
10. itive Control 3 2 1 5 10 1 5 ml 100 2 95 5 3 8 000 15 000 rpm 5 PCR 2 PCR 2 95 5 http wwwr takara bio coJp 4 RR139A 2 PCR 1 1 g PCR 23 ul 4 3 dH2O 1 Reaction Mix Fast 12 5 ul 100 x SYBR Green 0 25 ul Primer Mix VT ipaH invA 5 ul dH20 5 25 ul Total 23 ul 2 Positive Control dH20 2 ul
11. l Hi 8 Tube NJ300 0 2 ml Hi 8 Flat Cap NJ302 Thermal Cycler Dice SYBR Molecurar Probes Inc TIafd4R4 Tel 077 565 6999 Fax 077 565 6995 http www takara bio coJp v201510Da
12. ma RR139A TahaRa TaKaRa vV201510Da PCR EtHEC VT EHEC 4 pg PCR PCR PCR PCR PCR 1 EHEC 3 PCR 1 PCR SYBR Green Tm Tm EHEC VT1 2 79 5 81 5
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