Virus Test Kit (HIV, HTLV,HCV, HBV, ParvoB19)
Contents
1. IV Thermal Cycler Dice Real Time System 2 Thermal Cycler Dice Real Time System Auto Thermal Cycler Dice Real Time System Manual http www takara bio coJp 4 RR271A V V 12. NNA DNA NucleoSpin RNA 740955 10 50 250 Proteinase K 740506 DNA RNA DNase DNA RNA HBV Proteinase K DNA Proteinase K 1 1 Xx 106 2 350 Buffer RA1 1 Triton X 100 1 M DTT 3 5 ul 3 Proteinase K 1 590 ul RNase free water 10 ul Proteinase K 2 10 55 10 3 10 000 3 950 ul 4 0 5 Xx 475 ul gt 96
3. 5 4 NucleoSpin RNA Column 11 000 X g 30 11 000 X g 30 Collection Tube 2 ml 6 1 200 ul Buffer RAW2 11 000 X g 30 Collection Tube 2 ml 2 700 Buffer RA3 2 11 000 X g 30 Collection Tube 3 250 Buffer RA3 11 000 xg 2 Collection Tube 1 5 ml 7 50 ul RNase free 20 11 000 X g 1 11 000 Xg 1 50 ul 20 70 1 Proteinase K 50 mg vial Proteinase Buffer 2 5 ml
4. RR 1A IdRaRa Virus Test Kit HIV HTLV HCV HBV ParvoB19 v201508 6 HIV1 HIV2 HBV HCV HTLV1 amp 2 parvovirus B19 Thermal Cycler Dice Real Time System RT PCR Primer Probe PCR 6 RT PCR TaqMan TaqMan Cy i 3 TAMRA eo 7ag DNA 5
5. 2 BufferRA3 Wash Buffer RA3 Concentrate 4 gt 96 http www takara bio coJp 5 RR271A V 2 PCR V 1 PCR Primer Probe Strip 7 1 1 8 7 1 dH2O Positive Control Mix 7 8 1 8 2 x One Step RT PCR Buffer ll 125 100 ul Takafia Ex Tag HS 0 5 ul 4 ul PrimeScript RT enzyme Mix 1l 0 5 ul 4 ul or Positive Control Mix 7 or dH2O 5 40 wl dH2O 6 5 ul 52 ul Total 25 200 ul 2 Primer Probe Strip 7 1 Primer Probe Strip 7 2 1 7 25 ul 3 Flat Cap 4
6. TP700 TP760 NucleoSpin RNA 740955 10 50 250 Proteinase K 740506 VIIl TakaRa Ex 7g9 Thermal Cycler Dice TaqMan Roche Diagnostics GmbH PrimeScript sm Idf4R4 Tel 077 565 6999 Fax 07 565 6995
7. http www takara bio coJjp v201508
8. 3 exonuclease TaqMan 1 2 1 1 3 ac http www takara bio co jp 2 RR271A 12 Package 1 1 2 Xx One Step RT PCR Buffer Ill 625 u X 2 2 72 2 2 Ex Tag HS 5 U ul 50 ul 3 PrimeScript RT enzyme Mix 1I 50 4 dH20 1 ml 5 Positive Control Mix 7 100 ul Package 2 Primer Probe Strip 7 12 Flat Cap 12 8 blank Primer Probe Primer Probe Strip 7 Primer Probe
9. 1 HIV1 5 HTLV1 amp 2 2 HIV2 6 parvovirus B19 3 HBV 7 Human B actin Internal Control 4 HCV 8 blank 1 7 Primer Probe Primer Probe http www takara bio coJp 3 RR271A Package 1 20 Package 2 4 lI NucleoSpin RNA 740955 10 50 250 Proteinase K 740506 PCR Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Single TP850 TP870 Thermal Cycler Dice Real Time System Lite TP700 TP760 Thermal Cycler Dice Real Time System TP900
10. Primer Probe Primer Probe Je lt LN 5 Thermal Cycler Dice Real Time System V 3 PCR PCR Thermal Cycler Dice Real Time System RT qPCR Cycle 1 42 5 95 C 30 2 step PCR Cycle 45 95 C 5 56 30 FAM http www takara bio coJp 6 RR271A VI NIBSC 4 PCR HTLV PCR DNA NIBSCCode 1 HI
11. V1 1st1 S for HIV 1 RNA 97 656 500 1U HIV2 HIV 2 RNA 1st1 5 08 150 HBV 2nd WHO I S for HBV DNA 97 50 250 1U HCV 1st 1 S for HCV RNA 96 90 250 1U HTLV Plasmid 250copies Darvovirus B19 pB19 DNA NAT assays 2nd 1 5 99 802 2500 1IU HIV1 Fluore sones Frirary urywl 14 16 18 0 24 3 40 4 i 1 30 Cree 1 1 1 1 Cyeles http www takara bio co jp 7 RR271A HCV ryel n Flunrwwcwncm Frimamry Cu ht manmnmn nm a in 14 16 18 a 22 Cyeles 0 4 10 1 parvovirus B19 _ 5 E 1 G 35 30 Ln a tm i mn Fluone seanes Fimarny Lm D1 1 18 20 2 4 Cyeles http www takara bio co jp 8 410 1 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42 44 46 Celes 2 4 eb 10 pir 3 RR271A VII Virus Test Kit EBV CMV WNV RR272A Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Single TP850 TP870 Thermal Cycler Dice Real Time System Lite
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