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Contents
1. 11 1 2 DNase RNase DNase RNase DNase RNase2. DNA RNA RT RNA cDNA L LAMP Loopamp 3 Ss cDNA DNA RT Dynabeads GC Combo
3. D AB C D 12 2 LAMP
4. 80C 37 5 A 60C 30 8 2 75C 10 95C 5 1 x Proteinase K 50uL LAMP RT Loopamp RT PrimeScript RT Master Mix Master Mix 2uL RNase free dH O 5uL 37C 15 85 C 5 LAMP Inomata A et al Development and evaluation of a reverse transcription loop mediated isothermal amplification assay for rapid and high sensitive detection of Cryptosporidium in water samples Water Sci Technol Vol 60 2167 2172 2009 1
5. DNase RNase 3 1
6. P 6 2 1 2 80 C 1 37 C 1 5 1 x Proteinase K 50uL 60 30 2 75C 10 LAMP 95C 5
7. 13 1 Loopamp 1test 10test 2 x Reaction Mix RM 12 5uL 125uL Primer Mix Cry PM Cry 2 5uL 25uL ps7 DNA Polymerase 1 0uL 10uL Distilled Water DW 7 0uL 70HL Total 23 0uL 230uL 23uL lt cs 2uL Loopamp 1test gt 10test 2x Reaction Mix RM 12 5uL 125uL Primer Mix Gia PM Gia 2 5uL 25uL Bst DNA Polymerase 1 0uL 10uL Distilled Water DW 8 0uL 80uL Total 24 0uL 240uL 24uL 1uL
8. 1 5mL 1 5mL 1 5mL 1 5mL 9 OD OO OT 80 C 2mL 0 2mL DODD 0 2mL8
9. LAMP Cyyp ospo7gju77 Giardia DNA DNA 1 1 5 Cryptosporidium Giardia LAMP 1
10. 2 3 CO MN 4 5 Loopamp 20 L RT 5 L Distilled Water DW Positive Control Cry PC Cry 5 L Positive Control Gia PC Gia 5uL LAMP
11. O 1 Dynabeads GC Combo SC DB73002 Dynabeads GC Combo 1 kit 10tests 2 8 1 PET PET 100 PET 1L 10 20g 800mL EDTASNa 30g 1N HCI 1N NaOH pH7 4 1L Tween80 10g 2 1xProteinase K 20 20 C 1 20 1 test 600mAU mL Proteinase K 1 5mAU mL 0 125uL QIAGEN 1M NaCI 20mM 1 00uL 10 Triton X 100 0 1 0 50uL 1M DTT 2mM 0 10uL TE Tris EDTA pH8 0 48 275uL
12. RT 14 50uL 0 1M HCI 0 01 Tween80 10 i 10 10 50uL 0 1M HCI 0 01 Tween80 10 10 10uL 1M NaOH 2 xProteinase K 1 test 600mAU mL Proteinase K 3mAU mL 0 25uL 1M NaCI 40mM 2 00uL 10 Triton X 100 0 2 1 00uL 10 000 x 0 3 1M DTT 4mM 0 20HL TE Tris EDTA pH8 0 46 55uL 50uL LAMP 260uL Y 50HL 50uL LAMP 80C 37 5 50uL LAMP 15 2012 8
13. LAMP Loopamp 10 LAMP 4 O 1 2 o
14. PC 5uL LAMP LAMP DNA 20C 1 5mL 1 x 3
15. Dynabeads GC Combo LAMP LAMP 1 1 1 2 2 3 19 PET 2mL 10mL PET 2 2mL PET 2 10mL PET 1
16. 1 RT PrimeScript RT Master Mix Master Mix 2uL RNase free dH O 5uL 2 3uL 3 37 C 15 4 85C 5 LAMP 1 XProteinase K CD CO l Oo OO 2 LAMP Loopamp Loopamp
17. 3 RT RT PrimeScript RT Master Mix 4 LAMP LAMP Loopamp Loopamp 9 Dynabeads GC Combo 10 x A 1mL 10 x B 1mL 1 Dynabeads anti Cryptosporidium 100uL Dynabeads anti Giardia 100uL 1 2 PET 5 10mL x 2 2mL x 2 10mL x 1 1 x 10 1mL 3 1 rr 3 180 1 1
18. 1 2 x Reaction Mix RM 1 2 x Reaction Mix RM 2 Primer Mix Cry PM Cry 2 Primer Mix Gia PM Gia 3 Distilled Water DW 3 Distilled Water DW LAMP 4 Bst DNA Polymerase 4 Bst DNA Polymerase DNA 5 Positive Control Cry PC Cry 5 Positive Control Gia PC Gia 2x Reaction Mix RM Primer Mix Cry PM Cry Primer Mix Gia PM Gia Bst DNA Polymerase Distilled Water DW i Total 1test gt 10test 12 5uL 125uL 2 5uL 25uL 1 0uL 10uL 4 0uL 40uL 20 0uL 200uL 20uL 5uL RT DW
19. Loopamp 2012 8 24 3 2 0302 2 4 19 3 30 0330006 LAMP pp P 3 pp P4 it P5 P6 7 Pg LAMP P9 LAMP P 10 11 0 P 12 13 P 14 15 1 eewrt P 14 2 P15 Cryptosporidium Giardia 18S rRNA
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