PCR 実験の手引き
Contents
1. 2014 10 20 TaKaRa TEL 07 543 6116 FAX 07 543 197 TEL 03 32 1 8553 FAX 03 32 1 262 TEL 07 565 6969 FAX 07 565 6995 14102. Tr MT IT 6 iT ma ET tf y 3 1XxXTAE DNA
3. BO CO OE
4. NCBI NCBI NCBI web page NCBI http www ncbi nlm nih qov N RI Search An Databases National Center for ology Informati human gapdh Clear NCBI Home Welcome to NCBI Popular ResourceS Site Map A Z The National Centerfor Biotechnology Information advances Science and BLAST RSDOIES5 health by providing access to biomedical and g
5. 3 PCR PCR PCR 1 3 5 LC smosse MM IIUEI DI PCR 1
6. 1 DNA Hot Start lahaha PCR 5 1 PCR DNA DNA DNA DNA PCR DNA
7. PCR Uracil N glycosylasetUNG TaKaRa PCR Carryover Prevention Kit code 6088 PCR FCR DNA A U C A G U A T C A G T T A UNG 125 lt 10 IT N EE LL LL 5 1 1 1 1 1 EEE IE EE Ri 1 a TT A GG E TAG TE A
8. IMC Web Page http www takara bio co jp research htm TakaRa Enslih 0 D Bp DIR EE a We http www insilicobiology co jp Primer 3 http frodo wi mit edu DNA PCR NCBI Primer BLAST http www ncbi nlm nih gov tools primer blast index cgi7LINK_LOC BlastHome Primer3 BLAST Primer
9. 3 3 9 7 3 CAGTmmmmmmm 3 mm CA GT mm gt lt ea se gt CAGT 5 3 3 T 3 T 0 GTCA 9 Eee CA mm TGAC mm gt CAGT 3 GC GC 40 60 G A T CO GCC AT
10. BLAST BLAST Basic Local Alignment Search Tool DNA 18 Tahaha Re 3 DNase EDTA DNase TE buffer TE buffer 10mM Tris HC1 pH8 0 0 1mM EDTA
11. 1 1 5 ml 11 PCR PCR 1 11 33 5 wl 368 5 1 10X PCR 5 0 1 55 0 1 dNTP Mixture 2 5mM 4 0 wl 44 0 1 F Primer 1 0 wl 11 0 1 R Primer 1 0 wl 11 0 1 PCR 0 5 wl 5 5 wl Total 45 0 wl 495 wl PCR PCR 0 2ml
12. PCR BPB XC bp 0 5096 196 296 396 496 590 15 000 5 000 2 600 500 250 200 0 5090 196 296 396 496 590 1 800 650 200 80 40 25 DNA bpy 10 100 1000 10000 100000 EE 20bpDMA Ladder ES S0bpDNA Ladder 1 0 bEPNMA Ladder g 3 bh PMMA Ladder lil 35 bp DNA Ladder FREE 500 bpHNA Ladder PR IkbDMNA Ladder FREE z5kpDMNA Ladder FE 100bp DNA Ladder DL2 000 DNA Marker 50 10 000 bp DL OOO DNA Marker IE bp bp DLs 000 DNA Marker K174 Hinclldige 174 Hae Il dige EE 4 HY Marker HI IEE IE Bstp I digest a A EcoT14 digest
13. PCR om PCR 0 2m1l 45p 1 5 1 3 3 5 1 4 PCR PCR PCR PCR PCR Tahaha Re 3 DNA UV DNA 1
14. 20 60 1 3 70 0 5 g ml Um 20 Tahaha Re 5 mm
15. Q 0 0 0 0 4 A T G C DNA Deoxyribonucleic acid A G T C 4 A T G C DNA DNA 1 5 DNA DNA DNA DNA 3 DNA
16. buffer dNTP deoxyribonucleotide 5 triphosphate DNA dATP dGTP dCTP dTTP 4 Mg Tahaha Re 4 1 DNA PCR DNA DNA DNA
17. PCR A 15 LC smosse gt 1 PCR 1 2 3 1 PCR GenBank DDB
18. DNA 95 1 3 1 30 LC smosse EL Tm 5C Tm Tm DNA 50 DNA DNA
19. DNA lt CS IafdR4 PCR MM IIUEI 9 Ne UV DNA Eo 1XTAE 0 5 g ml XTAE SYBR Green I 15 UV DNA
20. DNA 2 DNA DNA A T G C dNTP 1 DNA DNA 5 9 DNA PCR DNA 1 DNA DNA DNAK 2 DNA DNA 3 RNA 4 DNA Eee DNA 3 5 DNA dNTP 6
21. 1 5 ml 0 2 ml 8 8 1 5ml 0 2 ml PCR OO 1 5 ml 0 2 ml IafdR4 PCR 1 HHHHUHLH PCR 2
22. gt UV 2 LC smosse MM IE 9 5 PCR PCR PCR X 8 PCR X X X 1 2 A gt
23. DNA 5 3 3 57 94 gt 98 DNA 5 3 2 DNA 50 65C DNA DDNA DNA DNA A T G C 5 3 3 5 5 3 5 50 65 C A T G C cy DNA p p 5 5 DNA DNA 3 DNA DNA
24. 5 3 DNA DNA DNA 5 3 5 CH2 DNA DNA QO lt DNA 37 DNA 7 Ho P 0 TIT U gt 3 sy Cb a DNA QO QO 4 Tahaha Re DNA DNA PCR DNA DNA DNA DNA DNA MP DNA RNA
25. DNase EDTA DNase 2 PCR PCR PCR PCR PCR 1 DNA A PCR 12 IdfaR4 RE A 20 0 2ml 8 Di LL
26. DNA DNA ES DNA DNA TAE buffer 2 PCR PCR PCR UVW DNA
27. 20 IafdR4 PCR EE EE 1 2 1 160 ml SS 1XxTAE 160 ml 9
28. DNA DNA RNA DNA DNA PCR DNA DNA DNA DNA PCR DNA RNA PCR DNA DNA DNA DNA PCR DNA DNA DNA PCR 1 DNA DNA DNA
29. Tm 1m 55 60C Tm Tm Tm melting temperature DNA DNA DNA PCR lahaha PCR EL Tm nearest neighbor Tm Tm Tm CC 60 8 0 41X GC 500 n Tm CC 4X G C 2 X A T 35 2n GCo GC A G T C
30. 2 1XTAE buffer Tris Acetate EDTA buffer 20 50 gt Tris hydroxymethy1 aminomethane 242 g 57 1 ml 0 5M EDTA 100 ml 1L 50 X 50 1 XTAE 10mg ml Ethidium bromide EtBr 10 mg ml DNA DNA SYBR DNA UV
31. DNA DNA DNA 2 PCR 6 DNA PCR DN per tete 3O Tahaha ee
32. LE LA Tahaha Re PCR PCR Polymerase Chain Reaction DNA DNA 100 PCR Polymerase Chain Reaction DNA 100 PCR PCR DNA PCR
33. EE TP100 528 000 PCR TaKaRa 96 well PCR Hi Plate 12 40 700 upid 2plus EDIEETTTEETTT CE EC Agarose L03 TTAKARA 1 000 bp DNA MA 5003B y78 000 200 bp 20 000 bp h _ DNA PrimeGel Agarose LE 1 20K 5800A 100g 24 000 TAE Buffer 39 300 1 200 bp TBE PrimeGel Agarose PCR Sieve Buffer 39 5810A 100g 48 000 100 800 bp DNA PCR TaKaRa PCR Carryover Prevention Kit 200 46 000 L18 Gradient Thermocycler This product Model TP600 is licensed under U S Patent Nos 5 525 300 5 779 981 6 054 263 6 962 821 and corresponding Toreign counterparts thereto
34. 20 200 1000 DNA PCR DNase RNase PCR DNase RNase PCR 8 DNase RNase 0 2 ml Hi Iube Dome Cap NJ200 0 2 ml Hi Iube Flat Cap NJ202 0 2 ml Hi 8 Tube NJ300 Tahaha Re 0 2 ml Hi 8 Dome Cap NJ301 0 2 ml Hi 8 Flat Cap NJ302 Vortex
35. 1 2 1 3 2 3 4 4 1 3 4 1 lt 8 1 DNA
36. 2 DNA DNA DNA 3 gt 4029RNMO 4 DNA 4 DNA DNA DNA DNA 5 DNA 3 dNTP DNA lahaha PCR EE EE 2 PCR 1 DNA DNA DNA 94C 98C
37. 1kbp 1 25 40 DNA dNTP DNA 2 PCR DNA DNA 2 DNA pH Mg DNA
38. X lt PCR PCR 40 50 60 70 80 90 ITCTaCG CG ATG ATTTaaiGCATG TGCTG AAAGTTGGCGGTGCCGG AG TGCGC TCACCGC ll ILM DNA 3 Agarose LO3 5003 Prime Agaros
39. 5 Tahaha Re DNA DNA 4 57 cs 3 laa 5 3 5 68 72 DNA DNA 3 sy DNA 7 7 5 3 5 a 3 3 3 n 2 n DNA 100 57 3 n 2n NYA EE 100 SU 3 PCR 1 PCR DNA DNA DNA DNA
40. 2 gw DNA 5 3 DNA Tks GflexTM 3 5 exonuclease co c DNA 3 5 exonuclease Po1 1 PCR e 0 DNA DNA 5 3 5 GATCTG 3 CTAGATCGGAT 5 DNA G 3 5 exonuclease 3 5 exonuclease q earcT 4 DN SS A 5 GATCT gt l
41. A EcoT14 MBEI II dige EE readp trruse A Hind II digest pHY Marker 28 Tahaha Re 4 PCR 1 PCR PCR PCR M 1 3 4 0 1 PCR 2 PCR 3 PCR 4 PCR RO wr 2
42. 22 Tahaha Re
43. 500bp 1kb 2kb 3kb 4kb 5kb skb Skb 9kb 10kb 15kb 20kb 1XTAE 1X TBE PrimeGel Agarose GOLD 3 40K BOObP 1kb 2 kb 3 kb 4kb BEB gkB kb kb 10kb 15 kB 2 kb mi PrimeGel Agarose LMT 1 20K LMT 1 20K GAT 500 bp 2 kb 3 kb 4kb gkB kb kb 10kb 15 kB ih kb ZI 1 kb TEkb ms LT 2 Okb TO TN bp 1 000 15 000 bp gu0 TOO BB 500 10 000 bp 300 8 000 bp 250 7 nOO bp 200 6 000 bp OD 5 OD bE 100 3 500 bp 100 3 000 bp 0 OD bp 50 2 000 bp 40kb O00 40000 bp 2 000 20 000 bp 2 000 22 000 bp 1 500 12J000 bp 1 000 15 000 bp 1 0009000 bp A ODD bp 500 6 000 bp 0 5M0O0D bp 0 3M00D bp 40kb 500 20000bp 500 12 000 bp 300 16 000 bp 300 BOO bp 250 10 000 bp D0 4 OD bE 200 5 000 bp 150 3 000 bp 1 2 500 bp 100 2 000 bp 50 1 500 bp 5 15000 bp Tahaha ee 1 kb PrimeGel Agarose PCR Sieve 25bp 50bp 100bp 15Obp 200bp 250bp 300bp 400bp 500bp 750bp 1000 bp 1 250bp 1500bp 500 1 500 bp 400 1 200 bp 3 1 200 bp Prime AEarose go Pi Re 150 600 bp 50 500 bp 30 250 bp 20 250 bp A00 1 200 bp 100 1 200 bp 150 BOD bp Primetiel Agarose a gl EE EE EE 20 500 bp 20 200 bp 10 300 bp 100 bp 5n0 1 500 bp 00 1 000 bp 0 700 bp FTimmelG
44. PCR DNA mRNA miRNA PCR DNA A lt gt 6 DNA 6 PCR gt gt 6 PCR RE 6 qPCR 6 gt mRNA miRNA LC smosse gt 1 DNA DNA DNA Deoxyribonucleic acid DNA DNA DNA
45. Tahaha PCR DO DNA DNA PCR PCR PCR EGE 3 gt 1 DNA 0 4 2 PCR 6 3 PCR 7 4 9 5 PCR 1 4 gt 1 POR LL 16 2 PCR LL 19 3 3 2 4 4 PCR 28 5 29 as 32 lahaha PCR
46. dU plus dNTP Mixture FCR PCN PCR dTTP dUTP PCR 0 UNG PCR DNA 3 LC smosse 9 oR 32 Tahaha Re _ PGCR A4 PCR TP350 96 PCR IP600 858 000
47. e LE 1 20K 5800A Prime Agarose PCR Sieve 5810A Tahaha Re ee DNA PrimeGel PrimeGel PrimeGel PrimeGel PrimeGel PrimeGel PrimeGel PrimeGel Agarose Agarose Agarose Agarose LMT Agarose Agarose LE Agarose Agarose GOLD LMT 1 20K PCR Sie PCR Sieve LE 1 20K 1 20K GAT LMT 1 20K PCR Sieve 3 40K GAT ve HRS GAT 0 3 0 75 0 75 0 6 296 2 590 2 090 1 896 1 596 1 596 200 bp 500 bp 200 bp 200 bp 20 bp 10 1 500 20 kb 40 kb 20 kb 20 kb 1 500 bp 1 200 bp bp GAT pA O ol o 2ms O O Oo ol GAT Genetic Tested LC smosse EL LUI UEI HI HH DNA PCR 1 kb PrimeGel Agarose LE 1 20K LE 1 20K GAT
48. el Agaross 1m0 500 bp LT FPCR ET TO i000 bp 10 150 bp 10 50 bp 30 bp 13 LC smosse MMN II DI 5 PCR 1 Pol 1 o PCR DNA 2 PolI 5 3 exonuclease PCR 3 dA Taq EmeraldAmp a 3 5 exonuclease PCR PrimeSTAR Tks Gflex 3 5 exonuclease 5 3 exonuclease 5 3 ga 3 5 exonuclease X O 5 3 exonuclease X 3 dA Taq PrimeSTAR
49. enomic information Bookshelf Gene Chemica ls amp Bioassa Aboutthe NCBI Mission Organization Research RSS Feeds Genome Data amp Softy Nucleotid DNA amp RNA OMIM A A Get Started Protein D amp Struct PubChem Se 1 PubMed Genes amp Expression Tools Analyze data using NCBI software SR Downloads Get NCBI data or software PubMed Central How To s Learn how to accomplish specific tasks at NCBI SNP Genomes amp Maps Submissions Submit data to GenBank or other NCBI databases NCBI News Hamalnav IE Tahaha Re 2 PCR DNA DNA 20mer mer dNTP 20 PCR
50. t 3 CTAGATCGGAT 5 DNA DNA 3 57 exonuclease Pol I 5 GATCTA 3 CTAGATCGGAT 5 DNA 4 Tahaha Re 3 Hot Start PCR Hot Start PCR DNA DNA DNA PCR PCR DNA PCR lt DNA DNA
Download Pdf Manuals
Related Search