PDF:9MB
Contents
1.2.
3.
4. N WwW NN NN NN NN N LL
5. 26 2 gt 50
6. Em lgE 2
7. oo
8. 1 RA 3 3 Fl rl ET A Te
9. 13 23 13 3 15 79 2 K Fujino H Funatsuki M Inada Y Shimono and Y Kikuta 2001 Expression Cloning and Immunological Analysis of BuCkwheat Fagopyrum esculentum Moench Seed Storage Proteins Journal of Agricultural and Food Chemistry 49 4 pp 1825 1829 3 K Tanaka K Matsumoto A Akasaka T Nakajima T Nagasu Y likura and H Saito 2002 Pepsin Resistant 16 kD Buckwheat Protein is Associated with Immediate Hypersensitivity Reaction in Patients with Buckwheat Allergy Internatio
10. a 7
11. 1 2S 2 7 8S 11S 2 Fagopyrum excentum
12. 6 7 JA lt 3
13. gt PP es ne ys 1 lgE 23 1
14. 3 201 1 62 45 30 23 20 16 20 19 7 7 8 9 8 9 5 6 71 66 14 _ _ ee Ee
15. L
16. 1 UL 2 1 4
17. i Si a rt Fs Pe ee We re 1 rir 100 2
18. 30 2 BN 2
19. L 4 1 ss mr 4
20. 12 16 22 28 32 1 4
21. lgE 2000 Park JW 2 9 2011 IgE Satoh S lgE 2 2 pH 4 1 4 7 5 4 6 2 7 4 OS 9 4 4 6 7 4 4 3 kDa 24kDa 9 lt 19kDa b 20 1 16kDa Fag e 1 4 Fag e 3 14 4 Fage 2 8kDa 10kDa 2S Fag e 2 homologue
22. http www kadomaru com UMW dD EE
23. 1 7 0 13 mg g
24. 9 2 24kDa Fag e 1 16kDa Fag e 2 FDEIA
25. Fag e 1 Fag e 2 Fag e 3 3 Fag e 1 13S 8 22 kDa Fag e 2 2S 16 kDa Fag e 3 19 kDa Fag e 2 20 40 65 90
26. http www5a biglobe ne jp lt minemoto 1 K b S 1 2 3 4 5
27. iC 0 2 100 g A EC 2 SC mew Se lt HN _ De
28. 7 20 25 6 28 2 CD B N AR IgE
29. uo a eee ee 0 22
30. 31 ID VY OD VW SN OQ O Xj ET hk 2 ab mw Ri i
31. lgE 3 Fag e 1 22 24kDa Fag e 2 16kDa lgE 37kDa 25kDa lgE 2 IgE
32. 1 g 10 ug g g 1 Mean SE 3 relative standard deviation RSD 7 8 RSD 10 1 n 3 Mean SE hg g
33. 18 1 O 10
34. 1 NJ9c 14 20 1
35. 1 1 1 1 2 1 3
36. lgE ELISA 4 lgE 1 37kDa 25kDa 2 3 IgE kDa 180 0 i 50 Ws i 37 25 ma 20 15 10 kDa 1 100 Fag e 1 a Fag e 2 2 100 WW Fag e 1 Fag e 2
37. 2 ELISA PCR Polymerase Chain Reaction 2 PCR ELISA ELISA 6 1 1
38. Fag e 16kDa kDa 16 000 http www allergome org 24 28
39. lt 1404 25 7 21 25 8 29 40 25 9 12
40. 2S Fagopyrum tataricum Fag e 1 Fag e 2 Fag t 1 Fag t 2 Fag t 1 13S 24kDa Fag t 2 16kDa 2S Fag t 10kDa
41. Feg e 3 19kDa e 29 26 Fag e 10kDa lgE 60 Fag e 10kDa BkDa 16kDa 18 kDa 2S Ara h 10 IgE
42. gt gt 39 A BB 1 1 1 17 1 0 g 19 mL 15 100 3 cm pH pH6 0 8 0 pH 23C 3000 g 20 min
43. 7 OOF SNR OTISNE RR ER 117 IGG 2 G0 EE SOLS SS 40 I _ 34 EA EE EE Oe i to 32 coll a 0 18 _ _ 2 13 11 11 9 10 4 5 2 0 1 mm 0 6 y 7 gt ES 3 3 036 260 5 4 775 297 2 4 234 265 3 132 290 9
44. lgE WHO IUIS Http www allergen org Fagopyrum exclentum 3 1 1 2 3 Fag e 1 Fag e 2
45. 400 2 3 FASPEK ELISA Enzyme linked immunosorbent assay 5 1 FASPEK 1 2 lk SS YY
46. 2 3 RSD 10 9 1 7 1 4 1 2 10 3 32 2 ug mL 1
47. ES SIcherer SH JACI 108 881 2001 7 DK 20 A i VO Us 2 lt 60 46 se 2 5 3 3 3 9 2
48. 2012 2013 2013 2012 2003 2013 10 2011 2011 2011 2011 2008 2003 2009 2008
49. 21 Research of allergy 1970 WHO FAO Codex
50. 100 uL 25C 1 300 uL 6 100 uL 25C 30 300 uL 6 100 uL 25C 10 100 30 415 nm 34 3
51. 20 2 IgE ECL X lgE ELISA PBS ELISA 50 2 HRP lgE TMB 1M ELISA 490nm 29 2
52. a A DD 0 YE RY Sh 1 2 A
53. 1 2004 Hygiene hypothesis 1 11 4 pp 455 460 1998 9 1998 9 47 1 pp 26 33 Imamura T Kanagawa Y EDbisawa M 2008 IO OO A Survey of patients with self reported Severe food allergies in Japan Pediatric Allersy ana Immunology 19 3 pp 270 274 Park JW Kang DB Kim CW koh SH Yum HY Kim KE Hong CS Lee KY 2000 Identification and characterization of the JI major allergens of buckwheat Allergy 55 11 pp 1035 1041 6 2011 lgE 7 18 2 pp 103 109 7 Yoshioka H Ohmoto T Urisu A Mine Y Adachi T 2004 Expression and epitope analysis of the major allergenic protein Fag e 1 from buckwheat Journal of Plant Physiology 161 7 pp 761 767 8 Satoh R Koyano S Takagi K Nakamura R Teshima R 2010 ldentification of an lgE Binding Epitope of a Major Buck
54. 2 1 1 e e 1 os Sa 1 4 v v 2 1 2 v v 30sec 3 APRO SDS 100C 5 SDS PAGE 200V 95 CBB PVDF
55. Es kl Li EE LE 1 Pr Eh 1 Ee jr nd E a re 1 11 FL EEhln IPSJEUIFE2ETM dn T 1
56. 1 se NT 1 1 1 2010 gt es Re ZS EE tig BIR 99 0 20 40 60 80 100 AtoZ 10 p gt 8 CC RCE OR i
57. OAS 2 2 lgE et 1
58. 2013 2012 2013 2013 Vol 31 No 17 2004 2010 9 2012 2010 2010 AtoZ 2009 2011 2012
59. gt Ny a lt Ee A RR D D 7 AtoZ Se 0 5 10 _ 80 90 es 10 20 a 1 5 309 5 30 p 8 25 ss
60. 1 2S 16 18kDa BWp16 Fag e 2 Ara h 2 9Fag e 3 Ana o 1 Jug 2 7S Mi lgE RAST Fag e 1
61. 1 1997 7 0 4 1 9 2000 1 3 0 22 3 9 2008 Imamura 3 4 11 319 7 20 3 1 40 34 31 30 ININNG NRRT1HHNNT RINSRITNNININIT IT NTNNRNS STRTI
62. yy Na I a A ek i
63. 26 2 25 26 2 2 25 J 3 1 1
64. e 4 lgE ELISA 1 2013 63 2 2005 0 400 106 4 0 300 pp 444 450 IE 3 2008 in vitro Wsug Dermatology 7 3 0 000 pp 320 327 0 600 0 500 0 400 0 300 0 200 0 100F 0 000
65. 1 1 1
66. 5 Fag e 1 lgE 1 13S 148 13S o 8 6 24kDa 8B Fag e 1 IUIS Fag e 1 Fag e 2
67. 54 25 9 26 68 400 ES 1 125 g 145 g 160 g 210 g 1 170 g 220 g 28 L
68. AE 7 2 AtoZ 0 SS CN tk W S ZS ca
69. AtoZ 6 OQ NN 1 50g M 12 2010 1 30 35g 1 17 18g HS 16 7
70. 28 L 0 90 g 0 52 g 0 66 g 0 29 g 0 72 0 36 0 41 0 14 3 3 RSD 6 1 RSD 2 n 3 mg g mg g ug mL Mean SE RSD Mean SE RSD Mean SE RSD 54 4 1 2 3 8 S28 4 4 322 1 5 9 2 76 0 0 7 1 6 505 02 Oy 18 4 0 2 1 5 91 7 06 1 2 7 2 15 3 8 2 EUCI 27 45 88 8
71. 2012 2011 2012 2012 NHK 26 2 21 101 0051 2 4 3 TEL 03 3264 6265 Web TYTT
72. RX ES UR 2010 4 i 1
73. 0 16 0 007 0 24 0 007 0 021 0 001 Do OSG 2 4 8 4 X10 613 1 3 2 3 7
74. 0 9 60 7 2 8 7 9 OS 06 10 9 40 oo 36 3 n 3 ug g ug g Mean SE RSD Mean SE RSD 0 24 0 02 18 5 0 63 0 002 0 68 9 2 OWNS 2 1 2 11 8 0 08 1 1 7 9 0 13 2 8 45 0 15 0 004 6 1 N D N D 50 0 047 0 008 30 2 40 N D Not Determined 7 9 12 0 ug g
75. HH er Te rt EE EMDL ET ROC OR ane a ea abs ri LT EL 7 O ce EE bm LE tatty a a mg ye 1HF 3 TS 16 1 10
76. nal Archives of Allersy and Immunology 129 1 pp 49 56 4 S Y Choi J H Sohn Y W Lee E K Lee C S Hong and J W Park 2007 Characterization of Buckwheat 19 KD Allergen and lts Application for Diagnosing Clinical Reactivity International Archives of Allergy and Immunology 144 4 pp 267 274 5 14 11 6 1106001 6 Q amp A http www Caa gO jD foods qa allergy01_qa html 38 2013 Derma 2013 6 No 2051 2011 2013 NPC 2011 RR 2011 2012 1 2012 2012
77. wheat Allergens BWp16 by SPOTs Assay and Mimotope Screening International Archives of Allersy and Immunology 153 2 pp 133 140 9 Choi S Y Sohn J H Lee Y W Lee E K Hong C S Park J W 2007 Characterization of Buckwheat 19 KD Allergen and lts Application for Diagnosing Clinical Reactivity International Archives of Allergy and Immunology 144 4 pp 267 274 10 Matsumoto R Fujino K Nagata Y Hashiguchi S lto Y Aihara Y Takahashi Y Maeda K Sugimura K 2004 Molecular characterization of a 10 kDa buckwheat molecule reactive to allergic patients lgE Allersgy 59 5 pp 533 538 11 Zhuanhua Wang Zheng Zhang Zhuohui Zhao Gunilla Wieslander Dan Norback Ivan Kreft 2004 Purification and Characterization of a 24kDa Protein from Tartary Buckwheat Seeds Bioscience Biotechnology and Biochemistry 66 DD 1409 1419 12 P Chen Y F Guo Q Yan Y H Li 2011 Molecular cloning and characterization of Fag 2 a 16 kDa major allergen from Tartary Duckwheat seeds Allersy 66 10 pp 1393 1395 13 Sammut D Dennison P Venter C Kurukulaaratchy RJ 2011 Buckwheat allergy a potential problem in 21st century Britain BMJ Case Reports 2011 doi 10 1136 bcr 09 2011 4882 e 2 of allergy 1 scee
Download Pdf Manuals
Related Search
PDF:9MB pdf 9mb pdf 9 mb to 3mb converter