JP 2008-538284 A 2008.10.23 10 (57)【要約】 【課題】エストロゲン受容
Contents
1. LI LI I LI II II II II II II II II II II II LI LI LI II II LI LI LI II II II II II II II II II II II I LI LI II II II II II II II II II 0 LI LI LI II II II II II II II II II II II LI LI LI LI I LI LI II II II LI I LI LI LI II II II II II II II II II II II II LI2. Affymetri x Enzo Aff ynetrix I LI II II II II II II II L II LI II II II II II II II 0 00u II II II II II II II II I 2000000000000 T7 based Luzzi 0 000 Affynetrix Genechi pp D D D D 00020000000 Luzzi Pol II LI LI II II II 00 T7 based II LI II II II LI II II II II II II II II II II II II
3. LI I LI II II II II II II II II II II II LI II II II II II LI II II II II 900 nmn 0000 0 I LI LI LI II II II II II II II II II LI II II II II II II II I LI LI LI II II II II II II II II II II II II 00000000 0 0 I LI LI LI II II II II II II II II II II II II nnn I LI LI II II II II II II II II II II II II 00 II LI II II II II II II 000000 LI II II II II II I LI LI LI II II II II II II II
4. el 0 0 0 0 Moggs ER e associ ated binding sitel Vest Gr uvber ger 00 Sotiriou Enmert Buck II II II II II II II II II II 1000000000 MM 1 0 II II II II I I II II 00 s vYinmitt L Nakamura 000 Seth 1000 Ni shi date bulk tissue tumor sanple But probe sets psi ds L II II
5. L1 L3 LI L I E J P 2008 538284 A 2008 10 23 10 20
6. 20 30 40 50 23 2008 538284 2008 10 23 u ated protein 1 000 0 2 24 2008 538284 2008 10 23 ER ER L CM 1D 200670 at 1 03E 07 200747_s_at 3 24E 07 200811_at 7 35E 10 201030 x at 5 11E 07 201596 x at 1 92E 07 201795 at 1 12E 07 202035 s at 3 13E 06 202342 s at 1 14 06 x 202345 s at 2 83E 06 202554 s at 1 67E 06 202908_at 9 59E 07
7. 47 J P 2008 538284 A 2008 10 23 2 000 iu HHHHHHHHHHHHHHHHHHHHHHHHHHH 00 72 LI LI LI iu HHHHHHHHHHHHHHHHHHHHHHHHH 0000 2000 00000 HHHHHHHHHHHHHHHHHHHHHHHHHH 000 JUDU 48024 AAO1 2 CAO4 CAO9 HNI2 48029 07 BB20 FA15 48063 R32 55 616 525 54 002 40085 AA32 AA38 1186
8. 10 20 30 40 50 42 J P 2008 538284 A 2008 10 23 40
9. 2008 538284 A 2008 10 23 10 20 30 LI II II II II II LI II II II II II
10. 520000 10000000 0 00000000000 0 00000000000 000000000000000000000000 00000000000 LI II II II LI II II II II II II II II II II II II II II II I I I LI LI LI I LI LI LI I LI II LI I LI II LI I LI LI LI 000001011 III II III II III II II III II II II II II II II II IIII II II IIII II IIII II I II 10000000 I LI II II II II II II II II II II 100000000 I LI LI LI 0000 I LI II LI 0000 II LI II II II II II II II II II II II II II II II II II II II II II II II II II II II II II II II II II II II I II III L II II II II POE II II II II II II I 100000000 HHHHHHHH 000 LI I LI LI LI I LI II LI 10 2 J P 2008 538284 A 2008 10 23
11. 17 J P 2008 538284 A 2008 10 23 gner Software II II II II LI II II II II II II II II II II II II II II II II 0 Wagner Associates Mean Vari ance Opti zati on Appl i cati on s Mean Vari ance Opti m zati Library Markowitz sense 0000000 stock return a ement s 000
12. 50 14 J P 2008 538284 A 2008 10 23 drug di scovery mere presence 0000000
13. J P 2008 538284 A 2008 10 23 9 N m st in 3 H u 2x DI H H H gt z gt L I oO D O ni
14. I LI LI 20 30 40 50 J P 2008 538284 A 2008 10 23 12 10 I LI LI 2
15. S Plus software II II II II LI II II II II II II IIII 0 Insightful Corporation Wi ghted Voti Golub LI II II II II II II II II II II L Support Vector Machi nes Sup 0000000000600 Ranaswany K nearest Nei ghborsH Y 0 O O L3 L I maswany H LI II 0 0 0 0 0 II II II 0 J P 2008 538284 A 2008 10 23 19 10 I LI LI I LI LI mass n 4 c gt a
16. 50 43 2008 538284 2008 10 23 20 Gene Spring software 44 2008 538284 2008 10 23 0000 0000 MM 01278 100 100 MM 01455 100 037 E
17. Fox II II II II II II II Fernan Akhurst B LI II II II Metsuda II II II II II 0 II II W C I L B B II II II II ert Colas II 0 0 0 0 E nn ANS 8 si J P 2008 538284 A 2008 10 23 31 10 I LI LI I I LI II Khoo Mari nkovi ch 20 LI LI I LI LI I LI LI Tam m J P 2008 538284 A 2008 10 23 32 Hop III 10 20 30 40 A 888200 NN 1S ddJVd 19 5 268902 08 8LALEL 22000 SLLMM 3 X 966102 6c EMAX L 919
18. 37 04 2006 01 33 574 200601 37 00 2006 01 0 L3 L3 E EH Ez 46 35 00 37 04 33 574 37 00 000 J P 2008 538284 2008 10 23 AP BWG1 KE LS NA SD SL SZ TZ UG ZW EX AZ BY KG KZ M RUT TM EP AT BG CH CY CZ IK EE ES FI FR HJ LE S LT LT LU LV MG PL PT FQ SE SI SK TR OX EF B F G3 MG GN GQ GM NE SN TD AE AG AL AM AT AU AZ EB BG BR BBY 87 CH CN CR QJ CZ EK DM DZ EG E EG ES FI G H R HJ ID IL IN S J P KG KM KN KR KZ LC LK L R LS LT LU LV LY M MG M MY MM NA NG N NZ CM PG PH PL PT RQ RU SG SD SE SG SK SL SM SY TJ TMTN TR TT TZ UN UG US Uz VG W YU ZA ZM ZW 308021338 n gt oana LI L I 21349
19. LI II LI II II II II II II II II II II II I LI LI I LI LI II II II II II II II II II 0 LI II LI II LI II II II II II II II II II II II LI II LI II II I LI LI I LI II II II II II II II II II II II I LI LI I LI II II II II II II II II II II II um I LI LI II II I LI LI I LI II II II II II II II II II II II I LI LI I LI LI II II LI II LI II II II II II II II II II II II LI II LI II II II II II II II II II II II 000 LI II I LI II II II II II II II II II II LI II I LI LI I LI II II II II II II II II II II II LI II 10 20 30 40 50 J P 2008 538284 A 2008 10 23 I LI II II II II II II II II II II II 10 20
20. a a S A T amp EG E 4 1 YAH 0 006N 908712 261 v EUBx a crcvAeAlaA 24473Y 3 LSAN 192vv 1n v PLSAN 9 961 2 2 CA ACT LLPEIO INN ye 966507 961 E 09 4v08V ye X 8 9012 v6l LAELAE 689200 NN 19 067 002 26 1 66 gt ILIQddl ve 91202 cel 0 Z Z C C cege z LL OSPd VU 94 5 869910 INN LV6edAO 19 S 260221 161 SAKS 9 TEX 19v1 100682908 9SSV1 18 5 27212 061 2 44 4 lt 4 1 641041 6 2 6 161000 WN Ye 24 707 681 VI 5 98100029 VII 32789012 881 C F 0 0 ZL EIG 698ZZ Y ye 8Z0ZIZ 781 09 MV X n z C 4 SSO ANIVA 9911 910 NN 9dd 19 5 62902 981 v 901f14 HAERA 691810 901714 ye 198612 981 C 12 VY L 9 1 C 4 L OGAE 991020 NN ye vel UX 9 KOTL HAL ZYVNAI 3 X 68 902 281 IV AX 004000 19 210102 281 L LEMA 2 4 LdVON ye 8052121 181 YSKAOLYJYLOI L 702291 47 uv 325011115 081 BH lt FAT 7 LAKITE ye Sp 602 621 99W 0506LVIV 12 S 021602 821 ELMI EZELEE 260
21. ZO 92 200719 at 7 48 07 66 200804_at 2 13E 08 65 201037_at 1 46E 08 _ 93 201754_at 8 40E 07 100 202089_s_at 1 32E 06 67 202897_at 2 52E 08 62 202982 s at 2 58E 09 111 203287_at 2 95E 06 110 203773 x at 2 83E 06 83 204284_at 4 04E 07 73 204540_at 1 60E 07 97 204567_s_at 1 01E 06 90 204667_at 6 42E 07 64 204822_at 1 03E 08 105 205081 at 1 95E 06 63 205186_at 6 07E 09 112 206249_at 2 99E 06 88 207571 x at 6 14E 07 80 208788 at 3 37E 07 4 77 208873_s_at 2 82E 07 72 209114_at 1 54E 07 69 209122_at 2 87E 08 103 209324 s at 1 88E 06 96 209870 s at 9 47E 07 98 210397 at 1 23E 06 75 210845 s at 2 22E 07 85 211063 s at 5 32E 07 74 211967 at 2 12E 07 70 212276 at 4 99E 08 81 212501 at 3 47E 07 76 213634 s at 2 33E 07 4 94 213651 at 8 46E 07 101 214431_at 1 37E 06 71 215304 at 8 52 08 107 215329_s_at 2 27 06 89 216988_s_at 6 25E 07 4 108 217979 at 2 28E 06 95 218104_at 8 55E 07 87 218195 at 5 72 07 106 218239_s_at 2 22 06 104 218532 s at 1 92E 06 78 218534 s at 3 10E 07 91 218854_at 6 51E 07 109 218966 at 2 40E 06 10 20 30 40 000 00 99 84 79 86 68 82 102 219615 s at 219918 s at 220425 x at 221834 at 221934 s at 51158 at 60471 at 27 1 25E 06 4 21 07 3 22E 07 5 37E 07 2 72E 08 3 56E 07 1 46E 06 2008 538284
22. I LI LI 0 20 30 I LI LI formatted assayl Affymetri x Proset 10 40 50 Partek Di scover J P 2008 538284 A 2008 10 23 20 10 o ELO E E D EJ IE Ed m om
23. J P 2008 538284 A 2008 10 23 8 10 I LI LI I LI LI I LI LI LI I LI LI I LI LI I LI LI I LI LI I LI LI 20 30 I LI LI I LI LI 40 I LI LI I I I II II II I LI LI I LI LI I LI LI I LI LI I LI LI LI I LI LI bulk tomor data set 50
24. 30 40 50 J P 2008 538284 A 2008 10 23 5 I LI LI I LI LI LI I LI LI LI LI L LI 10 I LI LI I LI LI 20 30 I LI II I LI LI I LI LI I LI LI I LI LI 40 I LI LI LI LI LI LI JO a I LI LI LI
25. 10 20 allelic va LI LI I LI LI I LI LI I LI LI I LI LI n 30 CO 40 50 I LI LI I LI LI ri ati on nsti tuti vel y J P 2008 538284 A 2008 10 23 11 I LI LI 10
26. I LI II II II II II fold c I LI II II II II I graphical Dendogra Agilent Technol ogi es GeneSpri ng Part ek Partek Discover Partek InferH LI LI II II II II II II II LI LI LI LI LI II II II II II II II II LI LI LI LI II II II II II II II II II 0 I LI LI II 0000 II II J P 2008 538284 A 2008 10 23 16 10 20 30 di seased 40 LI I LI LI 50 I LI LI I LI LI I LI LI II
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28. 50 000 6 J P 2008 538284 A 2008 10 23 LI II
29. LI I LI LI II II II LI LI LI II II II II II II II II II II II I LI LI II II II II II II II II II 0 LI LI LI II II II II II II 0 II II II II n LI LI LI LI LI II II 00 II II II II II II II nnnm LI LI I LI LI II II LI LI I LI II II II II II II II II II II II I LI II II II II II II II II II II II I LI LI II II LI LI LI II I I II II II II 0 II II II II LI LI LI II II LI LI LI II II II II II II II II II II II 0000 10 20 30 40 50 41 J P 2008 538284 A 2008 10 23 I LI LI LI LI II II II II II II II II II II II II I II II II II II 1000000 III III II II II II L L L 00000 0
30. 203256_at 3 03E 06 203263 s at 3 49E 07 203453 at 1 17E 06 203712 at 3 09E 08 203909 at 3 51E 07 203928 x at 2 45E 10 204304_s_at 9 30E 09 _ 204508_s_at 4 40E 09 t 204623 at 1 11E 09 t 204872 at 7 34E 07 204915 s at 2 58E 06 205009 at 4 34 08 205044_at 7 53E 08 205225 at 1 99E 09 t 205376 at 3 00E 06 205548 s at 1 85 07 205862_at 1 24E 06 206392 s at 2 02E 07 206755 at 4 71E 08 206838_at 2 79E 06 207828 s at 3 22E 07 208682 5 5 33E 11 209191_at 4 94E 07 209460 9 44E 09 209602 s at 9 40E 08 t 209696 at 5 00E 08 209791_at 1 51E 07 210347 s at 4 60E 09 211712 s at 5 15E 07 t 212441 at 4 65E 07 212494 at 4 31E 1 1 212496 s at 2 66E 06 t 212638 s at 8 19E 08 212692 s at 6 49E 08 212744_at 4 72E 07 212956_at 1 46E 07 t 10 20 30 40 000 00 47 42 10 16 20 52 45 56 31 28 50 36 44 212985 at 214053 at 214440 at 214745 at 216092 s at 218211 s at 219197 s at 220016 at 220230 s at 220540 at 221203 s at 221920 s at 222125 s at 25 2008 538284 2008 10 23 1 12 06 5 43E 07 7 33E 09 4 94E 08 8 03E 08 2 94E 10 1 30E 06 7 64E 07 2 76E 06 2 98E 07 1 85E 07 1 20E 06 3 63E 07 7 43 07 10 26 2008 538284 2008 10 23 3 E R ER
31. 908900 WN 518 19 ObSOZZ 82 6 VELT 8956220 NN SD4NOM 1 5 805502 22 BI CALA ZE ML 69967071Y ye 6 602 92 2880V VIM 28807 VIH ye 956212 Sc ATE 962200 WN 887 1e S6 102 2 080900 INN Ld8x ye 079002 c 39 VIVO 69196 V tVvivo 5 209604 22 1 fi y 6 856 KA 16416148 1e S 869212 12 AM MAY GIVGIOMY 5V VI2 02 PREV vavo S Z 2 LLZLLO AN 19 61 QEZ2 t 2 ZO fSP 3A SIT 98909 vgall x s 269212 81 La 4ZcvYxYa 91 Y 4244 C 750920 ye 969602 LI ALILZYALYIMNMLAL NAZLU 14 1A_CICYAEYAMNL34 4 N Z99000 WN ye 0 L2 91 94 Cw xcg nzZcc ga Z NMELL cOSPd VE 247 292000 WN 982dAO ye 957902 91 601962 522600 NN 600502 vi 0Z00VV 948910 INN 0200VVIM 214602 CL KARGE Z LV 184 24v lvav 16 097602 e 1 210900 INN LNOdd xe 5 vOEPOZ LL G CUT ELE EHE ME dJ9 26122 vagya esop 0L BHRE 4AVLGAG VII EN 110 BEBE g 912080 IV 3 S V950L2 6 IX HANGA 21010028 ZIVO 19 8 80902 8 LHEECLGOA YT 5621000 WN 1853 622502 4 gt 41 922600 INN ie 629702 9 HESS YNY HELF 082100 NN ye 18002 S 8 MAX TEYAK 1 6 L
32. AKT 29035 12200 INN 29035 ye 16106 291 HESZ S Z 64 8bpLE04VY dIHONAS 16 S 92060 191 070V9 V 35VVII 4 81092099 ye 9 910Z 091 COL AENEA 616600 WN 19 889 61 MES 6 1020 INN Ye S 908612 8 2 HEIE A core 110408 8424NZ 16 S 6 60 Fl HHE 9V70VVIXI 68Z810 V 9p 20V VIM 32 97 aZe 109810 INN AZEMLS 19 989612 Srl HE C 0V2299 I LI aes 110222 6C IML 182 A fet 2608VV VIEIHO 3e 5 966604 LX 5 102202 cl 14 881020 INN ZUNYV 88922 90X0 2 CLN EL UNA Oll 025100 WN 108319 ye 026202 Sp An SOSZO WN W 841022 621 HB HAB 9080 6 01S14V Ye S 228212 821 TAFALT MAETVE 1 GL XI LL 880800 INN LNOSH 1 5 v9SIOZ El 1O 415 AVG 0685V Inv LOXOd ye 092512 981 2 Z oo 4 ZY G 2 gt 9 3 231 2 185 179122 Sel VI HHE 50401 44 E 662910 INN IVdSH W 212612 vel 0E Z9 116000 WN 5 O0 LOZ 221 XL 766910 INN 18 S 828212 cel J P 2008 538284 A 2008 10 23 37 000 00
33. 20 30 40 29 2008 538284 2008 10 23 LI LI LI 0 194 210648 x at 2 43E 06 188 210687 at 2 23E 06 168 210886 x at 1 54E 06 4 154 210942 s at 1 07E 06 180 211110 s at 2 03E 06 146 212314_at 8 36E 07 190 212442 s at 2 28E 06 196 212462_at 2 65E 06 10 181 212508_at 2 06E 06 174 212759_s_at 1 88E 06 156 212780 at 1 17E 06 161 212846 at 1 35E 06 136 213260 at 3 42E 07 162 213419 at 1 37E 06 197 214806_at 2 87E 06 173 215723 s at 1 85E 06 187 217028 at 2 22E 06 138 217823 s at 4 37E 07 20 132 217838 s at 2 60E 07 131 217929_s_at 2 55E 07 118 218236 s at 5 30E 08 184 218440 at 2 13E 06 119 218483 s at 1 17E 07 172 218489 s at 1 83E 06 165 218618_s_at 1 44E 06 124 218931 1 83E 07 157 219010_at 1 20E 06 129 219100 at 2 40E 07 E 30 134 219212 at 2 87E 07 177 219562 at 1 92E 06 145 219686 at 8 16E 07 148 219806_s_at 9 21E 07 185 219861 at 2 19E 06 4 153 219889 at 1 06E 06 139 220173_at 4 62E 07 191 220432_s_at 2 34E 06 166 220533 at 1 51E 06 141 220658 s at 5 50E 07 40 171 221562 s at 1 67E 06 4 135 221641 s at 3 40E 07 144 222011 s at 7 79E 07 116 52940 at 2 90E 08 L LI II II II II LI LI II II LI II II II II II II II II II II II II II 00000000 II II II II II II II II II II II II II L LI II LI II II LI II II II II II II II II II II II II II II II II II II II II
34. 2008 10 23 10 28 J P 2008 538284 A 2008 10 23 III I III 4 ER ER L CM VEND p f 193 200790_at 2 38E 06 113 200824_at 1 08E 09 182 201012 at 2 06E 06 176 201215 at 1 92E 06 133 201300 s_at 2 77E 07 164 201407 s at 1 39E 06 137 201564 s at 3 84E 07 150 201636 at 9 98E 07 128 201833 at 2 36E 07 152 201915_at 1 02E 06 120 201980 s at 1 29 07 158 202121 s at 1 22 06 192 202146_at 2 36E 06 142 202207_at 6 93E 07 140 202320 at 5 21E 07 189 202772_at 2 26E 06 t 117 203384 s at 3 26E 08 T 121 203682 s at 1 30E 07 115 203702 s at 2 17E 08 149 204688 at 9 46E 07 125 204751 x at 1 97 07 183 204785 x at 2 12 06 126 204881 s at 2 07E 07 159 205109 s at 1 27E 06 127 205300 s at 2 16E 07 122 205363_at 1 31E 07 186 205429 s at 2 21E 06 123 205471_s_at 1 65E 07 195 205996 s at 2 53E 06 167 206364_at 1 53E 06 170 206565 x at 1 61 06 155 208103 s at 1 15E 06 114 208358 5 1 05E 08 151 209025 s at 1 01 06 55 178 209170 s at 1 97E 06 175 209173_at 1 90E 06 143 209396 s at 6 97E 07 147 209494 s at 8 55E 07 130 209531 at 2 54 07 160 209631 s at 1 30E 06 n 179 209745_at 2 00E 06 169 210319 x at 1 59E 06 163 210466 s at 1 38E 06 10
35. ALIHM 9 Z C 6 4 64059 41 916700 INN 1558v 1e 5 199702 16 z X V 09 S Z 94 617V109V 2V V 19 5 0 8602 96 01 1209 9v2210 OIX31 38 01812 56 G 6 MAGO z Z CM 02756619 V 99I 1C 69612 v6 4 2 ZA XEB MOVYVUZA t 8r00 29X0 ye G 10Z 6 v61d vi HEISST Ea l S V0V9639 VidMS 15 612007 26 1 288910 WN ZLHVS 16 758812 16 IVYGAM NANG EC 960700 NN ye 199707 06 Z XM Z X ig VA 64 LOL 22789X 2VV I 3e S 886912 68 SE 12 878700 BEHOLO 15 X 19202 88 L LC 9 9 0 WN 11232090 ye 561812 18 ZELOOZAY Ye 8 98 HEISE 64 090028 LYON 3 5 690112 98 mg dse 621810 AdSV 19 S 816612 v8 DEAGTTLA EH La 4zcYNd C LIIC S0092N JEYLddd Te 7821707 28 LU E 61809009 7 955 04 ELGLOSIV 165007201 ye 89119 28 d99 9 6 X VX 1 22 89r9g IV 83832 1e 105212 18 oI3 vans IN34 9 13 lt C 14013 66695 L IV GTAO 16 881802 08 XC A Gy 829210 LNdOd X 90022 67 OSDA 970810 NN DSDA S bEG8LZ 8 8l S s c 200008 81 062
36. I II II II II II II II II II II II 0 I mcrotubul cytoskeleton organi zati on 50 30 J P 2008 538284 A 2008 10 23 I LI LI II II II II II II LI II II II II II II II II II 0 reti noi c aci
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38. 98 15149 15154 Seth et al 2003 Anticancer Res 23 2043 51 Simstein et al 2003 Exp Biol Med 228 995 1003 Sotiriou et al 2003 Proc Natl Acad Sci USA 100 10393 8 Spillantini et al 1998 Trends Neurosci 21 428 33 Su et al 2001 Molecular classification of human carcinomas by use of gene expression signatures Cancer Res 61 7388 7393 Sun et al 2005 Exp Cell Res 302 96 107 Tamimi et al 2004 Invest Ophthalmol Vis Sci 45 3904 13 van t Veer et al 2002 Gene expression profiling predicts clinical outcome of breast cancer Nature 415 530 536 West et al 2001 Proc Natl Acad Sci USA 98 11462 7 Wu et al 2003 J Biol Chem 278 15192 200 Wu et al 2003 J Biol Chem 278 51673 84 Yim et al 2003 Toxicol Pathol 31 295 303 00000 000000 I LI II II I II II I II II II II II II II II II II II II II II II II II II I II II II LI II II II II II II II II II II II II II II II II II II I II II II LI II II II II II II II II II II II II II II II II II II II II II II II II II II II II II II II I II LI
39. II II II I0 III II II II II II II II II ALT 10 II 1 II II II II I L L L E E EE E b B 0000000 II II II II II II II II II II I 20 L L L L I 1 000000000000 00000000000 nn m m 30 I II II II I I11 1 II 1 II 0000000 Sequence Li sti ng 40 s nna T i An c gt EE ELE gt si nmn T E A Lu e ERE gt nmn F 11 10 11 breast carci noma Parl Hn HHEH H 50 7 J P 2008 538284 A 2008 10 23
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41. II II II II II II II II II II II II II II I 00000000 LL I II II I II II II II II II II II II II II II II II II II II II II II II I 000 II II II II II II II II II II II II II II II II II II EE II II II II II II II II II II II II II II I LI II II LI LI II II II II II II II II II II II II 0 00000 0000 II LI LI II LI II II II II II II II II II II II II II II II II II II II II II II II II II II II II II I II LI I II LI LI II II II II II II II II I I LI II II MI II II I II II II II II II II II II II II II II II II II II II I II II II LI II II II II II II II II II II II II II II II II II II II II II II II II II I II II LI II II II II II II II II II II II II II II II II II II II II II II II II II II II II II II II I II LI II II II II II II II II II II II II II II I 00000000 00000000000000000000 0000000 LI II II II II II II II II II II II II II II II II II II II I LI MI II LI LI II II II II II II II II II II II II 0 00000 0000 I II II II II II II II II II II II II II II II II II II II II II II II II II II II II II I II 40 JP 2008 538284 A 2008 10 23
42. Z Z C Z 688 LVES9ETW 8 15 19 5 260912 r Gs HSE ENLA 0 8 ldVW 39 X 826602 SURE 81921 V 2035VVI ie s 289802 2 amp 4CYNEEAFXd10gm cccs 8668709V LONIL 19 6 212 L Hare HE pisd fgg S x J P 2008 538284 A 2008 10 23 33 a a CALL 818800 ALL 228702 v9 Z9F800 NN 19 981502 9 2 cYIE4Vvoo AC 4 128900 WN 8214 7 Ye 5 286202 29 ES 86 104 14445 3 S 9860202 19 GEHE e d EL 46N 861100 INN eHQO ye 952602 09 COMSOL CA 4 3 998600 WN ye 92502 69 LIOO WN Sdgv4d 3 S SpEZOZ 85 Y Z X L 671900 WN 61 91 16 868902 219 S6EGODM MAERA 090770 WN GS6ESODW ye 910022 99 gz REA XY fuouunr ZLLLEZMV ae 5 96 212 SG LL YGAM A ERETTE AYS 1 98208 LIXOS 28 5 916702 9 M N S X E lt amp 1 05 29 IV ENLSD S 799202 96 lt lt 10170 WN 5 110810 25 MEX 19345 899710 INN 18345 ye 298502 16 YA SLVIA 620810 INN 251 18 5 602122 08 ACE AX v lt NAL 880100 INN VINNOS 16 6V 02 ZBZ 3 38 1
43. 0 30 LI LI 40 50 J P 2008 538284 A 2008 10 23 13 10 20 30 40
44. 10 L MIBN ALY GRE 265700 INN 5 L pSOZ 221 CIGVO L ASAMITERKCANSGULIER 996800 NN 15 696904 cel XE duod v 7 rca 522200 NN s 289602 121 sey WN 11684 s 086102 Oel 1281815 91080 WN 16 S 68812 611 dA C ZEH 818900 W S 967812 811 220200 NN Iv51059 S 211 U BB 41 1181 ALGA 9 G80VV daIDIS ye 06291 911 nz cdHa un czcurz cnc cse 126 01 S 202202 SIL EJAN GEHA ERA GEL dAN 8 096600 AN SLD S 892802 UNA LICYALY SAKL L 6914 258000 WN 14159 Ye 28004 EL ALFA AFA LF MBE VR ICAA 122000 NN E LMEdVIN Ye 6bZ90Z LA AL 899900 WN LV I ye 82202 LLL AVA 217000 WN VaAT 3 X ELLEOZ 01 S9AZXZES 824810 NN OSOAN 996812 601 ELMAX M3L L M Y 66 viO NN Ye 6 6 12 801 22 lt 9 Z Z Z i X HEC 787150 IV 23656216 e S 622912 401 196010 WN dddl ie 5 622812 901 HY 1 116100 WN ye 80504 SOL 25104714 WA EEA 000610 WN 261027
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47. 42810 xe 5 206202 nn 62251138 LINDO ye 986212 Lv Z Z 900900 INN IS4M ye 806202 9r 2 4 403 X X 16020 NN 238025 3 S 761612 _ S 2 3xnd3a rA4 n Fi C 08900029 18 S 621222 rr 493 amp 6ZXvV ZX TAATELE 500 00 Ye ZL8v0z v MZ LOA x lt SVY SESS00MV 19 826612 6V 068850028 5 21212 006200 WN ye X 060102 8 N L 79920009 8OFOSN 18 161602 6 V RIAN LAM ZLLEL8IV vsag Ye Z1Z 86 GRAY CECOVVIM 586980 C COVVIM 19 212 16 2 GEJ 194 1939 dOSW 16 S 026122 98 B N IL 6 656900 INN 976216 606502 S 6 439 X X LG cv 6 4S29Iv 6339H3V 19 S 29202 I 98 1900 WN LYWNN 19 5 2400 554 2007 098 3 MERC X 34 12 961900 NN 5 828 02 c 2 992 660959V IC 622910 INN 2458 2 Ye S 062022 LE E J P 2008 538284 A 2008 10 23 34 000 00 10 20 30 40 19 Sr6 4n 19330 Ye 62012 86 I xX ZXx
48. 500 NN ye 299612 221 M LOA Z LEYEH SYA ozgvH ESEPLO NN ye 912102 9 1 a r amp x uncae PRE 2 Tc C 6 X Z 1988804V 221602 85 1 LG 5 1 282 LOEOZIV 16 5 6877212 CA 4 CY Ia 8 0 2942rv 16 5 202912 221 GUL CACNLCAS3L 21600029 5 687812 HAEE 2 EMS LTL 8018804 ye S Z9GIZZ 1 1 EVINS 084900 WN ye x 6596908 0 1 TV GEZGEA z x usu 11 68q 19 X 612012 691 L gd SY LO08V LdVESdI 19 X 988012 891 vi vAx nz4cAeckx 9 8710 INN v9 902 291 S8 1114 MEHREN 8 WN S8 1f 14 19 6650044 991 L3 sm D 38 J P 2008 538284 A 2008 10 23 REFERENCES US Patent Nos 5 445 934 5 532 128 5 556 752 5 242 974 5 384 261 5 405 783 5 412 087 5 424 186 5 429 807 5 436 327 5 472 672 5 527 681 5 529 756 5 545 531 5 554 501 5 561 071 5 571 639 5 593 839 5 599 695 5 624 711 5 658 734 5 700 637 6 004 755 6 136 182 6 218 114 6 218 122 and 6 271 002 US patent publication 20030194734 Akhurst et al 2001 Trends Cell Biol 11 544 51 Ammanamanchi et al 2004 J Biol Chem 279 32620 5 Binder et al 1985 J Cell Biol 101 1371 8 Chen et al 2005 J Biol Chem 280 4632 4638 Eckert et al 2004 Cancer Res 64 4585 92 Emmert Buck et al 1996 Science 274 998 1001 Erlander et al 2004 WO 2004 07014 Fer
49. II II II II II II II II II II II II O Trizol method 0 0 I trogen CA I LI II II II II II II II II II L L LI LI II II 0 GeneChip Hul Affynetrix CA I LI II II II II II II II II 0 O ZEI SS Axiovert 135 L II LI II II II II II II II 0 P A L M Mcrolaser Technol ogi es Germany 0 Cryocut 1800 Rei chert J ung cryotone Cambri Instruments Germany D CH LI II LI II II II II II II II II II II II II II II II II II II II II II II II II I 000 M r Technol ogi es Germany LI II LI II II II II II II II II 00000000000 I LI LI LI LI II II II II II II II II II II II II II 0 Si is VI LI LI II II II II LI II II II II II II II EosinY Sigma St Louis HE BT OG SE III d 1 III I II II II II II II II II II II II II II II II II II II 0 LI LI II II II II II II II II II II II II 0 0 0 0 RNeasy Qi agen Germany DOO LI 0 II 0 Zym spi n col um Z
50. JP 2008 538284 A 2008 10 23 19 JP A 11 2008 538284 P2008 538284A UJARE 2 10 23 2008 10 23 51 Int Cl F1 3 C12N 15 09 2006 01 C12N 15 00 ZNAA 48024 C12Q 188 2006 01 C12Q 1 68 4BO29 C12M 1 0 2006 01 C12N 15 00 4BO63 AG1K 39 39 2006 01 C12M 1 00 A 4C085 A61P 35 0 2006 01 AG61K 39 39 BARR RAR 8478 21 2008 505567 P2008 505567 71 505060347 86 22 18 4 3 2006 4 3 85 19 12 3 2007 12 3 Veridex LLC 86 PCT US2006 013004 O07059 87 w02006 108135 87 18 10 12 2006 10 12 47 33 31 60 668 005 33 Technology Drive 32 17 4 4 2005 4 4 Warren NJ 07059 U 33 05 S A 54
51. T E fi CI ca E I g g Hra 0616 LI BA Y iE E BN pa N 30 5 55 ST li lt IL YA mH gt m IM X BB W ER d E NH ih lt H 35 06 E z gt 40 50 D LI II II II II II II II II II II II II I II I LI LI II II II II II II II II II II II II II I II I LI I II LI II II II II II II II II I LI LI LI LI 10000 LI LI 00000600 LI LI L LI LI II LI LI II II II II II II II II II II II II II I LI II II II II II II II II II II II I
52. VSA 101 098 A MM 008923 m AKO22697 CARAS 100 104 MM 003564 100 035 _ MM 04280 MALES 100 035 NM 000194 APRITE 100 nes _ _ n 018213 100 108 Emm NM 055 1 00 085 AL136935 100 084 gt MM 000190 P esr 100 1 16 Amps JEDE 0 35 EEE 31 AF112207 050 121 AK022531 0 58 130 E AI075450 0 144 seni 017704 008 075 00642 APPIE 005 am MM 079 sai 008605 ETEF om 128 E MM 00 24 PPPG 002 078 809 1 D 2000 4000 6000 ER LE 0000 64 EX L L CM m nL CM 45 J P 2008 538284 A 2008 10 23 LI LI LI II 2008538284000001 app 5I nt d 0000 0000 0000 81 71 71 71 71 74 74 74 72
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