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Cycleave®PCR Bacillus cereus (CRS gene) Detection Kit

1. RNase H RNA B188 COBH CRS FAM ROX CRS Qu gt om gt gt
2. PCR l http www takara bio co jp 4 CY221 V 1 2 1 PCR 1 NGKG 500 ullcES S3 A 2 O CECS AA BETA 3 5 ul PCR 2 PCR 1 SCD 2 10 35 2 1 3 ml 1 000 rpm 100 x g 1 1 0 1 2 ml
3. PCR PCR Hot Start PCR 7aKaRa Ex Taq HS RNA DNA RNase H OB
4. 2 DNA OUT Q gt PCR QO ROX FAM Primer Probe Mix DNA 3 ND Q http
5. Epoch Biosciences Inc Epoch Biosciences Inc A B C SAVE Tel 077 543 6116 Fax 077 543 1977 NANOGEN http www takara bio co jp NOTICE TO PURCHASER LIMITED LICENSE L4 Quencher This product is for measurement of amplification detection for research use only in life sciences research industrial and envir
6. Threshold Baseline Manual http www takara bio coJp 10 CY221 S Dice ii ma ma B WIESE I CRS Target FAM IC ROX 10 View Well Table B ens 305 anr EE inven cma 30588 ont 3 Man onp at LES EM 35436 EI ic Wa nan Pare Cate Pod i agam n pore ac prc ie mm Tn Eg hii StepOnePlus Real Time PCR System Life Technologies ROX target ROX IC FAM ROX target http www takara bio co jp 11 CY221 VI 1 2 ROX as FAM
7. 3 1 2 3 PCR PCR Auto PCR Manual PCR
8. 2 CRS 1 Q 2 x Cycleave Reaction Mixture 12 5 ul 1x CRS Primer Probe Mix 5 X conc 5 ul 1X CRS Positive Control 5 yl 1 a C dHo 2 5 ul Total 25 ul k1 20 ul 3 2 3 2 CRS Positive Control 1 0 2 ml PCR http www takara bio coJp 5 CY221 V 3
9. DNA PCR PCR DNA Koz CRS 7 dH20 J amp ERZK C S CRS Positive Control CRS DNA ll 20 C
10. 2 PCR fei li 44 a 3 Siep PCR Hold e 0 2 3 Cycle 1 d 95 C 1015 1 3 step PCR fo Cycle 45 m 95 5 55 C 1015 i 72 203535 a5 5 53 0 72 nns ppg nn 3 r F 3 BIA FO Fe Hz Ils http www takara bio coJp 6 CY221 4 Omit woe BEC DXX FE ERES VERE VUES r E B DDD gt 4 UHKH re K ROX 2 5 l 2 FAM IC BHD ROX
11. 12 000 rom 13 000 X g 3 3 500ulTE buffer 10 mM Tris HCI 0 1 mM EDTA pH8 0 95 5 4 5 Ul PCR PCR PCR 1 NGKG x 2 SCD V 2 1 CRS CRS 1 1 og 20 ul 1 ul
12. gt http www takara bio coJp 8 CY221 6 gt lt N gt P gt OK OUT gt Posi Nega ND PCR Error B 1 OUT gt
13. Bif amp ld Auto http www takara bio co jp 7 CY221 2 NC PC NC lt N gt FAM ROX FAM ROX IC m a n taj H T der j t Jit 6 2 4 WOM 7h52 4 18 18 20 22 4 24 2806 12 14 YO YAN YO i de EE 5 ET MON 870 12 M 18 708 20 272114 2400 22 340 a 40 42 a YO ia 4 i le PC P gt FAM ROX FAM ROX IC Se 2 de gs B 4 n 21 pi 5 gi zi 5 4 B8 5324 18 18 20 22 M 34 28 i 12 a a a a a ARTI SEPET j4 hi le 3
14. CY220 Cycleave PCR Sa monella Detection Kit Ver 2 0 CY205 Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Tlme System Lite TP700 TP760 96 well Hi Plate for Real Time NJ400 Sealing Film for Real Time NJ500 Plate Sealing Pads 9090 48 well snap plate NJ700 Flat cap for snap plate NJ720 0 2 ml Hi 8 Tube NJ300 0 2 ml Hi 8 Flat Cap NJ302 0 2 ml 8 strip tube individual Flat Caps NJ600 Cyclea
15. PCR 3 PCR 3 ne do Sik Thermal Cycler Dice Real Time System 5 E Applied Biosystems 7500 Fast Real Time PCR System Life Technologies tt Thermal Cycler Dice Real Time System 1 CycleavePCR Kit gt SHARUBIISE BEA C E EEG HT CycleavePCR Kit SNPs X NE CycleavePCR Kit Thermal Cycler Dice Real Time System Software PM M Plus Minus Assay gt IMhermal Cycler Dice de a System
16. CRS CRS CR CRS 2 2 CRS Positive Control NENNEN ROX a FAM CRS CRS CRS CRS 4 3 3 a ROX dl FAM CRS CRS CRS CRS 2 3 1 CRS x2 DC Ce
17. 3 PCR 4 CRS CRS CRS Positive Control X5 http www takara bio coJp 12 CY221 VIL VIII IX 1 Yabutani M et al 2009 Letters in Applied Microbiology 48 698 704 2 Agata N 2002 nternational Journal Of Food Microbiology 73 23 27 3 Agata N et al 1994 FEMS Microbiol Lett 121 31 34 4 Schraft H et a 1995 Applied and Enviromental Microbiology 61 98 102 Bacillus cereus CRS gene PCR Detection Kit RR132A Cycleave PCR O 157 VT1 VT2 Typing Kit CY222 Cycleave PCR O 157 VT gene Screening Kit Ver 2 0 CY217A B Cycleave PCR Vibrio tdh gene Detection Kit
18. II PCR Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Software Applied Biosystems 7500 Fast Real Time PCR System Life Technologies 95 4 200 ul 20ul 10ul http www takara bio co jp 3 CY221 IV 1 6 V PCR
19. www takara bio co jp 9 CY221 Applied Biosystems 7500 Fast Real Time PCR System Life Technologies 1 Advanced Setup New Experiment 2 Experiment Properties Quantification Standard Curve TaqMan Re agents Other Other Include Melt Curve 3 Plate Setup Define Target Target Name CRS Reporter FAM Quencher none 4 Plate any Define Target Target Name IC Reporter ROX Quencher none 5 Define Samples NC PC 6 3 4 6 Plate Layout Passive Reference none 7 Instrument E m ali Siaga Cyeling Stags 9 Cycle 95 3 step PCR Cycle 45 95 C 5 55 C 10 72 C 25 ipn 1 8 Start 9 Analysis HHA Amplification Plot Target
20. V EZ RNase Hlc amp 5 YE es Bll http www takara bio coJp 2 CY221 L 25 ul 50 Q1 2 x Cycleave Reaction Mixture 2 X conc 625 ul Q2 CRSPrimer Probe Mix FAM ROX 5 X conc 250 ul 93 dH20 1 ml 4 CRS Positive Control 150 ul 30 1 2 X Cycleave Reaction Mixture PCR Buffer dNTP Mixture CRS Primer Probe Mix FAM ROX
21. A the sale lease license or other transfer of this product or any material derived or produced from it B the sale lease license or other grant of rights to use this product or any material derived or produced from it and C the sale lease license or other transfer of kits which include this product W NANOGEN L34 B cereus This product is covered by the claims of U S Patent No 7 264 959 and its foreign counterpart patent claims M40 Thermostable RNase H This product is covered by the claims of U S Patent No 7 422 888 and its foreign counterpart patent claims M46 PCR Cycleave This product is covered by the claims of Japanese Patent No 4022522 http www takara bio co jp 14 CY221 http www takara bio coJp 15 CY221 Takaka 7 Tel 077 543 6116 Fax 077 543 1977 http www takara bio co jp STII 7A ARS 4 v201403Da
22. onmental testing including food industry but excluding bio terrorism and bio warfare non human animal diagnostic testing forensic testing and providing services to third parties who do not use the services for the purpose of a providing patient management or care and in which the results of such services are not included in patient records and b providing bio terrorism or bio warfare testing and specifically excluding without limitation I any human clinical therapeutic or diagnostic uses and animal clinical and therapeutic uses including any use of the results of any testing performed with any product for patient management or care or the use of the results of the services for patient management or care and Il any research or services where the results of any test or assay are used for patient management care or otherwise in making therapeutic or treatment decisions for a patient A portion of this product is subject to proprietary rights of Epoch Biosciences Inc and are made and sold under license from Epoch Biosciences Inc under the patents and patent applications US20020155484 WO0142505 WO02099141 US10 113 445 US09 876 830 and corresponding patents issued in other countries There is no implied license for commercial use with respect to this product A license must be obtained directly from Epoch Biosciences Inc with respect to any proposed commercial use of this product and commercial use includes but is not limited to
23. ve TaKaRa Ex Taq Thermal Cycler Dice http www takara bio coJp 13 CY221 Epoch Biosciences Inc m lk Epoch Biosciences Inc US20020155484 WO0142505 WO02099141 US10 113 445 US09 876 830
24. waa CY221 Tahaha Cycleave PCR Bacillus cereus CRS gene Detection Kit v201403Da Bacillus cereus 2 LC MS CRS

Cycleave®PCR Bacillus cereus (CRS gene) Detection Kit

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Cycleave&reg;PCR Bacillus cereus (CRS gene) Detection Kit

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Cycleave®PCR Bacillus cereus (CRS gene) Detection Kit