PDFファイル - 医薬品医療機器総合機構
Contents
1. 6 TaqMan 48 7 PCR DNA DNA 1 3 5 12. DNA H 6 H
3. 1 4 3 TaqMan 48 DNA DNase DNA DNA 5 000 ppm 0 5
4. TaqMan AV PC Pos avium MAV 47 intracellulare TaqMan IN PC Pos M intracellulare MIN 47 avium 1 DNA 2 DNA DNA PC_ INVALID
5. 70 5 000 ppm 0 5 2 2 1 3 5 lt 1 13
6. 1 2 8 2 2 24 Exp TaqMan MAI 96 1 Longo M C et al Use of uracil DNA glycosylase to control carry over contamination in polymerase chain reactions Gene 1990 93 p 125 128 2 TaqMan MTB 2007 58 p 331 337 3 TaqMan MTB MTB DNA
7. 47 avium DNA 520 nm 485 nm MM intracellulare DNA 575 nm 540 nm 715 nm 680 nm 1 1 2 TaqMan 48 Result 1 TaqMan 48
8. Mycobacterium avium DNA Mycobacterium intracellulare DNA Mycobacterium avium Mycobacterium intracellulare g mL NH Isonicotinic acid hydrazide 5 7 PCR TaqMan 48 EB Ethambutol dihydrochloride 1 7 Polymerase Chain Reaction PCR RFP Rifampicin 7 99 PCR M avium DNA M ntracellulare DNA RA Pyrazinecarboxamiide OND 16S rRNA M Kanamycin monosulfate 2 0 a N 2 ER CE Maviom BR 2 SM S
9. MAV MIN MAV MIN Invalid 2 PC_ INVALID 2 1 fi DNA IALC DNA
10. 5 6 74 8 0 5 1 3 5 000 ppm 0 5 2 1 E 121 20 i 5 000 ppm
11. Mcobacterium avium Mycobacterium intracellulare 3 80C 2 8 C FOOO 2 1 1 My
12. 4 NALC 2 TaqMan MAI 1 TaqMan MAI MAI MMX 8X0 46 mL KY436BUBZBIO KY703BIOBUBZ 500 L 100zL DNA KY1223FBH4 12 500 g 10 DNA KY1351HXBQ8 100 L 2 5 dATP 60 45
13. 5 3 TaqMan MAI N UNG DNA DNA 1 dUTP PCR DNA EDTA PCR
14. 2 5 A 5 000 ppm 2 DNA 6 5 000 ppm 0 59 7 TaqMan MAI TaqMan MYCO 0 09 w v TaqMan MYCO TaqMan MAV TaqMan MIN 0 05w v 4
15. NSR zip es 0 1 mol L Na citrate 50 mL 4 NaOH 50 mL 0 5 g N Acety L Cysteine 24 2014 11 7 2014 8 6 22100AMX00675000 15 mL 1 5 3 000 g 20 4 C 2 2 NALC NaOH 15 5 25
16. GE Healthcare Bio Sciences Corp Carnegie Mellon University URL Web http www roche diagnostics us patents 4 4 0 7225512 001 D 05031516001 07
17. 1 M avium DNA 140 10 2 734 98 6 874 886 99 1 874 882 2 M intracellulare DNA 86 1 3 2 788 99 7 874 877 88 1 A 1 791 99 8 879 881 1 3 F o
18. 30 15 25C 1 1 TaqMan MAI MAI MMX 1 TaqMan MYCO MYCO Mg 200zL TaqMan MYCO MYCO IC 50 7 L 10 15 12 2 8 2 MAV 2 TaqMan MAV MAV C 50zL 200L 100 7L 60 C 45 100 L 2 8 8 MIN 2 TaqMan MIN MIN C 50L 200L
19. 7 8 9 10 4 2 2 8 E 4 5 25C 11 D 2 A L 5 000 ppm 0 59
20. 2 5 dCTP 100 L 2 8 4 70C 2 5 dGTP 6 2 5 dUTP 2 Z05 DNA N UNG 3 2 TaqMan MYCO MYCO Mg 8X0 2 mL 3 TaqMan MYCO MYCO 1IC 8X0 1 mL 5 2 mg mL 10 4 TaqMan MAV MAV C 1X0 75 mL MM gz 47 intracellulare NALC 5 TaqMan MIN MIN C 1X0 75 mL 47 avium M intracellulare NM f EN 3
21. 13 4 E Nase DNA 3 1 2 3 DNA 5 000 ppm 0 59 DNA 4 DNA 5 000 ppm 0 5 DNA
22. 1 1 1 5 2 CDC NALC 2
23. PBS pH6 8 5 TaqMan MAI 3 000 g 20 4 PBS pH6 8 1 mL 500 g 1 lt 100xL 20 1 2 2 Me 1 BAL ss a 3 2 NALC
24. 100gL 60 45 100 L 2 8 8 2 2 1 1 K K K 24 2 K K 3 K K 4 K 5 50L 200L 7 8 2 9 II
25. K 50zL K K K 3 K 90 3 TaqMan 48 ID
26. 2 gt 3 4 2 3 6 RW 2X25 mL RL 3X6 mL RN 2X5 mL 0 1 5 mL 1 50L 100 L 200L 500gL 12 13 K K 14 15 16 8 60 3 TaqMan 48 9 PCR TaqMan 48 20 v3 3 21 TaqMan MAI Test Defnition 22 K 23 K
27. 2007 34 5 p 443 44 105 0014 2 6 1 0120 600 152 105 0014 2 6 1 0120 600 152 2 NSK PCR GE Healthcare Bio Sciences Corp Carnegie Mellon University
28. 24 25 v3 3 1 TaqMan TaqMan 48 CD File 2 4 3 2 TaqMan 48 1 2 3 17 CT13 CT13R 18 DTU 2B K 1 24 1 1 1 1 K K K
29. K 24 K 1 2 5 20 23 24 6 K K K K K K 50 L 2 2 8 2 2 2
30. TaqMan 48 TaqMan 48 K K ID 2 3 gt CTM MAI K K K
31. cobacterium avium DNA 1 Mycobacterium avium DNA 5 PCR Hit Rate X 100 95 TaqMan MIN b TaqMan MAV 2 Mcobacterium avium DNA 10 PCR TaqMan MIN 4 b TaqMan MAV 4 2 Mycobacterium intracellulare DNA 3 Mycobacterium intracellulare DNA 5 PCR Hit Rate X100 95 TaqMa
32. n MAV b TaqMan MIN 4 Mycobacterium intracellulare DNA 10 PCR TaqMan MAV 4 b TaqMan MIN 4 3 47 avium DNA 1 5 47 intracellulare DNA 1 5 3 4 2 899 784 BAL 8 27 30 50
33. treptomycin sulfate salt 25 30 N UNG DNA CAM Clarithromycin 0 483 0 801 DNA 4 RN a 0 A 4 477percg oss M bovis M microti M africanum SE A ORE DERE LN EN 33 1
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