pNC-HisF DNA
Contents
1. Amp NmR NOTICE TO PURCHASER LIMITED LICENSE L39 Brevibacillus Expression System 1 Brevibacillus Expression System related products were developed and manufactured by Higeta Shoyu Co Ltd and sold by TAKARA BIO INC 2 The use of this product is allowed for research use only 3 All rights of intellectual property related to this product are owned by Higeta Shoyu Co Ltd Note This product is for research use only It is not intended for use in therapeutic or diagnostic procedures for humans or animals Also do not use this product as food cosmetic or household item etc Takara products may not be resold or transferred modified for resale or transfer or used to manufacture commercial products without written approval from TAKARA BIO INC If you require licenses for other use please contact us by phone at 81 77 543 7247 or from our website at www takara bio com Your use of this product is also subject to compliance with any applicable licensing requirements described on the product web page It is your responsibility to review understand and adhere to any restrictions imposed by such statements All trademarks are the property of their respective owners Certain trademarks may not be registered in all jurisdictions v201307 pNC HisF DNA Code No HB122 Size 10 ug 0 2 OD Shipping at 20 C Store at 20 C 10mM Tris HCl pH8 02. KEIR L SUS 2 eel DA E 5 260bp A 4 m INO eae ae s De evace we ea EEC Pk pNC HisF DNA Aa L39 Brevibacillus Expression System 1 2 3 pNC HisF DNA Brevibacillus eV ac7 s pNC His
3. Brevibacillus Expression vector pNC HisF DNA Code No HB122 Size 10 ug 0 2 OD Shipping at 20 C Store at 20 C 2 years from date of receipt under proper storage conditions Lot No Concentration 0 2 ug l Volume 50ul Regarding protocol please refer to the product manual supplied with this vector Description pNC HisF is a shuttle vector of B choshinensis and E coli and this vector is a secretory expression vector Expression plasmid can be constructed by using coli as the host then transfered into B choshinensis cells The pNC HisF contains P2 promoter derived from the host cell wall protein HWP as its expression promoter and does not work in coli In B choshinensis cells P2 promoter works as very strong promoter enabling efficient protein production His Tag sequence is located at the downstream of the secretion signal peptide and Factor Xa cleavage site is inserted so that the His Tag can be removed from the fusion expression protein Form 10mM 1 mM Tris HCI pH8 0 EDTA Base pairs 5 260 bp Usage This is a vector for protein secretory expression using Brevibacillus Ex pression System a shuttle vector between B choshinensis and E coli Cloning sites of pNC HisF DNA Please refer to the other side of this sheet Map of pNC HisF DNA Lac operator P2 promoter sec signal peptide ent His Tag MCS ColE1 ori DNC HisF 5 260 bp rep
4. F P2 P2 g ew ac7 s His EA arg a leased Wty ordeal ean ae Hi NN His Factor Xa cIa pNC HisFDNA AAGGCGCCGCAACTTTTGATTCGCTCAGGCGTTTAATA
5. GGATGTAATTGTGAGCGGATAA 120 P2 35 P2 10 lac operator CAATTATTCTGCATGGCTTTCCTGCGAAAGGAGGTGACACGCGCTTGCAGGATTCGGGCT 180 SD1 TTAAAAAGAAAGATAGATTAACAACAAATATTCCCCAAGAACAATTTGTTTATACTAGAG 240 SD2 Hpa GAGGAGAACACAAGGTCATGAAAAAAAGAAGGGTCGTTAACAGTGTATTGCTTCTGCTAC 300 MK K R R V VN S VL LL L Aco TGCTAGCTAGTGCACTCGCACTTACTGTTGCTCCCATGGCTTTCGCTGCAGGTAGCCACC 360 L LAS A L AL TV A PM AF AA G 5 H Sec Signal peptide Signal cleavage Cloning site BamH Xbal Xhol EcoRI Kpnl ATCACCATCACCATATCGAAGGTCGTGGATCCGTCGATCTAGACTCGAGGAATTCGGTAC 420 H H HH H EGR His Tag Factor Xa Smal BstB Clal Hind ill SR ei CCCGGGTTCGAAATCGATAAGCTTCGGCATTATAG v201307 uoz a o E a 52Oaoes TaKaRa R DR
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