【課題】ポリペプチド
Contents
1.2. Int ILU d LU d LU d d LU d D l Ll LU 10 20 30 40 50
3. u 00000 u J D D C Hug n 00000
4. 10 20 30 40 50 2012 70751 2012 4 12
5. 10 20 30 40 50 2012 70751 2012 4 12 19 10 20 l
6. 10 20 30 40 50 18 JP 2 012 70751 2012 4 12
7. 93 2012 70751 2012 4 12 L1 DI DI DI DI DI DI DI DI L1 DI DI DI DI DI DI DI DI DI DI DI DI DI DI DI DI DI DI DI DI DI LJ 1 DI DI DI DI DI 1 DI DI DI L1 DI DI DI DI DI DI DI DI DI DI
8. 10 20 30 40 50 2012 70751 2012 4 12 35 IOD l 10 20 30 40 50
9. 0 1000 0 0000 1 DOAGKSPAGV RYHGGDEIIL QGPHWNVVRE APNDWYNILR QQASTIAADG FSAIWMPVPW 61 RDFSSWIDGG KSGGGEGYFW HDFNKENGRYG SDAQLRQAAG ALGGAGVKVL YDVVPNHMNR 121 GYPDKEINLP AGOGFWRNDC ADPGNYPNDC DDGDRFIGGE SDLNTGHPQI YGMFRDELAN 181 LRSGYGAGGF RFDFVRGYAP ERVDSWMSDS ADSSFCVGBL WKGPSEYPSW DWRNTASWOO 241 IIKDWSDRAK CPVFDFALKE RMONGSVADW KHGLNGNPDP RWREVAVTFV DNHDTGYSPG 301 ONGGQHHWAL QDGLIRQAYA YILTSPGTPV VYWSHMYDWG YGDFIRQLIQ VRRTAGVRAD 361 SAISFHSCYS GLVATVSGSQ OTLVVALNSD LANPGQVASG SPSEAVNASN GQVRVWRSGS 421 GDGGGNDGGE GGLVNVNFRC DNGVTOMGDS VYAVGNVSQL GNWSPASAVR LTDTSSYPTW 481 KGSIALPDGO NVEWKCLIRN EADATLVROW OSGGNNQVQA AAGASTSGSF
10. 50 2012 70751 2012 4 12 55 10 20 30
11. LU LU 0 0000 0000 DO E s E 00 000 00000000g 000 05000000 0 LU UULULEULULBUL LU 0 PU HJH 0 LU ULULULBULHULHUL 0000 0000 DO Doo LU LU 0000 DO LU 0 HPU HJH Il LU 0 D 000000000 0 0
12. 2012 70751 2012 4 12 23 10 l 20 30 40 50 ID
13. 49 2012 70751 2012 4 12
14. 50 2012 70751 2012 4 12 43 10 l DU 20 30
15. 10 20 30 40 50 2012 70751 2012 4 12 90 10 l n 20 30 40 50
16. 10 20 30 40 50 2012 70751 2012 4 12
17. pMD153 N33Y D34N G121F G134R A141P Y146G 11571 G158T S161A L178F A179T G223E 5229 H307L A309P S334P SAS1379 09 N33Y D34N G121F G134R A141P Y146G I157L G158T S161A L178F A179T G223E S229P H307L A309P 5334 W339E 1 2012 70751 2012 4 12 87 ID 10
18. 00000 gt 0000 67 JP 2012 70751 A 2012 4 12
19. pMD28 41 N33Y D34N G134R A141P 11571 L178F A179T G223A H307L S334P pMD44 D34N G134R A141P 11571 L178F A179T G121W H307L 5334 UUUUU 20 EH t 80 Betamy Phade 22 bas pMD28 4 N33Y D34N G134R A141P 11571 L178F A179T G223A H307L S334P pMD43 a N33Y D34N G134R A141P 1157L L178F 179 G121H H307L S334P I oog0 30 t4 80 Betamyl Phade lt bas pMD28 N33Y D34N G134R A141P I157L L178F A179T G223A H307L 5334 40 N33Y D34N G134R A141P 11571 L178F A179T G121M H307L 334P 50 78 2012 70751 2012 4 12 1000 bas pMD28 2 3 41 N33Y D34N G134R AI41P 11571 L178F A179T G223A H307L S334P pMD74 a 3 3 87 N33Y D34N G134R A141P I157L L178F A179T G121A H307L 8334
20. 10 20 30 40 50 57 2012 70751 A 2012 4 12 10 20 0000 no n een gt FWYHKMILVAGC WYHKREDCSTNQ 2222 C ED 40 nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 50
21. 20 10000 ID 30 0010 l 40 100000 DU 100 50
22. 40 50 2012 70751 2012 4 12 42 10 20 30 40 l
23. H MSHILRAAVLAAVLLPEPALA 100 100000000060 000 O EGGLVNVNFR CDNGVTOMGD SVYAVGNVSO LGNWSPASAV RLTDTSSYPT WKGSIALPDG QNVEWKCLIR NEADATLVRO WOSGGNNOVO AAAGASTSGS F A DHHBDBB 000 00 0000000 000 0 J AED en D 1000000000 er Do pr 2012 70
24. 25 2012 70751 2012 4 12 U jiunpdgudgulelulultklull lu D 9010
25. 2012 70751 2012 4 12 27 10 BU 20 30 40 50
26. 10000000000 0000 000000 2012 70751 2012 4 12 2 0 n 10 L l 20 30 40 50 IOD
27. 16 2012 70751 2012 4 12 l U l I 1 L U L L L iu l lI I L U U I L l 10000 II l U L U L i U 00000000000 U OUUOUOUUUUULUU l UI I L U LU 1 l l UI I l U L l l LLL IL 1 LL LL L L i l LLL BD LL LI LI l UI L U LI i l Ul L LI L i l lI LI LI i U 00000000000 00000000000 1000000000 l UI I l L L l U l lI I L U 1 L l Ll n U D t PU D l lI I l U U 1 L l l UI I L LI L i 10 20 30 40 50 2012 70751 2012 4 12
28. l 50 2012 70751 2012 4 12 9 10 2
29. 50 2012 70751 2012 4 12 48 10 20 30 40 50
30. 10 20 30 40 50 2012 70751 2012 4 12 26 10 l 20 30 40 50
31. 2012 70751 2012 4 12 24 ID 000 n 000 10 20 l l 30 40 50
32. 50 65 JP 2012 70751 A 2012 4 12
33. 40 50 10000 2012 70751 2012 4 12 45 10 20 C C I DU DU l 30 l
34. 91 2012 70751 2012 4 12 0000 a 000 U Ul U Ul U U l jiupgulglillul iu 1 0000000600 LU Dd 0000 PFHUUUUUUUUOD jiupgullulLliu C
35. 50 2012 70751 2012 4 12 22 10 20 30 40 50 n
36. C 20 C 30 G l 40 n 50 n C C C 2012 70751 2012 4 12 74 10 CU D IU 000 ID DU l DU l ID DU l
37. 3 JP 2012 70751 A 2012 4 12 10 20 30 40 50 4 2012 70751 2012 4 12
38. 6 JP 2012 70751 A 2012 4 12
39. 2012 70751 2012 4 12 32 10 20 30 40 50 2012 70751 2012 4 12
40. ee ee ee ee it sp GP iP ll O VO EI 0 00000 DOAGKSPAGVRYHGGDEIILOGFHWNVVREAPYNWYNILRQOASTIAADGFSAIWMPVPWRDFSSW TDGGKSGGGEGYFWHDFNRNGRYGSDAQUROAAGALGGAGVKVLYDVVPNHMNRFYPDKEINLPAG QRFWRNDCPDPGNYPNDCDDGDRFLGGEADLNTGHPQIYGMFRDEFTNLRSGYGACGFRFDPVRGY APERVDSWMSDSADSSFCVGELWKEPSEYPPWDWRNTASWOOIIKDWSDRAKCPVFDFALKERMON CSVADWKHGLNGNPDPRWREVAVTFVDNHDTGYSPGONGGQHLWALODGLIROQAYAYILTSPGTPV VYWPHMYDWGYGDFIRQLIQVRRTAGVRADSAISFHSGYSGLVATVSGSQOTLVVALNSDLANPOQ VASGSFSEAVNASNGOVRVWRSGSGDGGGNDGG US ERES B 00000 DOAGRSPAGVRYHGGDEIILQGFHWNVVREAPYNWYNILRQQASTTIAADGFSAIWMPVPWRDFSSW TDGGKSGGGEGYFWHDFNKNGRYGSDAQLRQAAGALGGAGVKVLYDVVPNHMNRFYPDKEINLPAG ORFWRNDCPDPGNY PNDCDDGDRF LGGEADLNTGH PQ1LYGMF RDEF INLRSGYGAGGFRFDFVRGW APERVDSWMSDSADSSFCVGELWKEP SEY PPWDWRNTASWOQQI KDWSDRAKC PVF DFALKE
41. Penninga D van der Veen B A Knegtel R M van Hijum S A Rozeboom H J Kalk K H Dijkstra B W Dijkhuizen L 1996 The raw starch binding domain of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 J Biol Chem 277 20 32777 32784 Sambrook J F E M T 1989 Molecular Cloning A Laboratory Manual 2 hk Cold Spring Harbor Laboratory Cold Spring Harbor NY Zhou J H Baba T Takano T Kobayashi S Arai Y 1989 Nucleotide sequence of the maltotetraohydrolase gene from Pseudomonas saccharophila FEBS Lett 255 37 41 30 00000000 00000000 40
42. 40 50 C 2012 70751 2012 4 12 46 10 20 l 30
43. 80 2012 70751 2012 4 12 1000 bas 1157L L178F A179T H307L 5334 SSM173 B6 1 5 76 N33Y D34N G121D G134R A141P l L178F A179T G223R H307L 5334 0001000 EJ LE E Ooo OOO n 00000 10000 0 x L L3 L3 Ooo 0O rrr Oo 0O Lr 0O rrr
44. bas pMD28 ro dE D34N G134R A141P I157L L178F A179T G223A H307L S334P EE D34N G134R A141P 11571 L178F A179T G121Y H307L 5334 Eh EJ D REED 1 F J E er ee bas 11571 L178F A179T H307L 5334 pMD3 2 8 IN33Y D34N G121D G134R 1157L L178F A179T G223A H307L 5334P EE E SEDE 100000 000 79 2012 70751 2012 4 12 bas 1157L L178F A179T H307L S334P SSM173 F6 1 6 118 N33Y D34N G121D G134R A141P
45. 2012 70751 2012 4 12 37 10 20 30 BU 1 00 0 40 50
46. 10 20 30 40 50 2012 70751 2012 4 12 41 10 20 30
47. 1000000000000 50 1 61 121 181 241 301 361 421 481 000 DOAGRSPAGV RDFSSWTDGG GYPDKEINLP LRSGYGAGGF IIKDWSDRAK ONGGOHHWAT SAISFHSGYS GDGGGNDGGE KGSIALPDGO 000 00 94 RYHGGDEIIL QGFHWNVVRE APNDWYNILR KSGGGEGYFW HDFNKNGRYG SDAQLROAAG AGOGFWRNDC ADPGNYPNDC DDGDRFIGGE RFDFVRGYAP ERVDSWMSDS ADSSFCVGEL CPVFDFALKE RMQNGSVADW KHGLNGNPDP QDGLIROAYA YILTSPGTPV VYWSHMYDWG GLVATVSGSQ QTLVVALNSD LANPGQVASG GGLVNVNFRC DNGVTOMGDS VYAVGNVSOL NVEWKCLIRN EADATLVROW OSGGNNOVOA QOASTIAADG ALGGAGVKVL SDLNTGHPOI WKGPSEYPSW RWREVAVTPFV YGDPIRQLIO SFSEAVNASN GNWSPASAVR AAGASTSGSF JP 2012 70751 A 2012 4 12 FSAIWMPVPW YDVVPNHMNR YGMFRDELAN DWRNTASWOO DNHDTGYSPG VRRTAGVRAD GOVRVWRSGS LTDTSSYPTW 00000 0000 OOOOH Ooo 000 1 121 181 241 301 361 421 DQAGKSPAGV RDFSSWTDPG DYPDKEINLP LRSGYGAGGF IIKDWSDRAK QNGGQHIWAL SAISFHSGYS GDGGGNDGG 000 00 RYHGGDEIIL QGFHWNVVRE APYNWYNILR KSGGGEGYFW HDFNKNGRYG SDAQLROAAG AGQRFWRNDC PDPGNYPNDC DDGDRFLGGE RFDFVRGYAP ERVDSWMSDS ADSSFCVGEL CPVFDFALKE RMONGSVADW KHGLNGNPDP QDGLIRQAYA YILTSPGTPV VYWPHMYDWG GLVATVSGSO OTLVVALNSD
48. 10 20 30 40 50 2012 70751 2012 4 12 50 10 20 30 40 50 2012 70751 2012 4 12 51 10
49. l 40 50 2012 70751 2012 4 12 63 10 20 30 40
50. 2012 70751 2012 4 12 38 10 IU 20 C 30 40 n 50 000 2
51. 83 2012 70751 2012 4 12 oa oa a 0O Moo oa L3 L3 L3 oa L3 oa L3 oa L3 oa oa L3 oa L3 EI oa L3 L3 oa L3 oa L3 DI MoO LE 3 ET ES EST EJ Neo j BRE aeri we AME E N33Y D34N G121F G134R A141P 1571 L178F 179 G223A H307L S334P A141P 11571 L178F A179T G223A H307L 5334 10 20 30 40 84 2012 70751 2012 4
52. 10 20 30 40 50 82 JP 2012 70751 A 2012 4 12 Moo 1 DI DI DI DI DI DI DI DI DI DI DI DI L1 DI DI oaa 0A SSM350 B11 306T pMD96 SSM350C12 H306G 10 100000 00000000000000 00 0000 00000 0 0000000000000 20 52 000 100000 10000 30 000000000 000000 000000000000000000 00000000 0000 000000000000000000 g umgamamuuuuuusussucuguuuuuuuuuuuuuuHuuuHuuHdt 10000 0000 40
53. BU l 40 50 30 JP 2012 70751 A 2012 4 12
54. l H 40 C 100000 C C 50 2012 70751 2012 4 12 73 10 000
55. hadebas 13 L178F A179T G223A H307L S334P 54 N33Y D34N G121F G134R A141P 1157L L178F A179T G223A H307L pMD55 S334P 10 20 30 40 50 76 2012 70751 2012 4 12 00000000 jUuluuliiullulill liiiiiulill 0 hadebas pMD85 5 1 115 N33Y D34N G121F G134R A141P pm T157L L178F A179T G223E H307L S334P PMD96 D34N G121F G134R A141P 11571 S161A L178F A179T G223E H307L 5334 E t 80 BetamyVP ARER hadebas 54 N33Y D34N G121F G1
56. C C 2012 70751 2012 4 12 36 10 20 30 40 l C C 50
57. 20 30 C C C 40 D C 75 2012 70751 2012 4 12 EE t 75 4 80 iBetamyl 222 Phadeba s Cei gt sei PSac D3 9 3 3 43 IN33Y D34N K71R G134R A141P 11571 L178F A179T G223A H307L D343E 334P PSac D14 24 N33Y D34N K71R 2875 61210 A141P 1157L L178
58. 30 40 DU 50 2012 70751 2012 4 12 54 10 20 30 40 l
59. 2012 70751 2012 4 12 000000000000 0000000 jiupgullglllkul iu jiunpgulllllul i jiupgulllllullu jiupgullllbil i
60. HE A M ono E Inn _ n 1000000 OUUU 1000000 10 20 30 40 50 2012 70751 2012 4 12 21 10 000 20 l 30 40
61. 40 50 l l 56 2012 70751 2012 4 12 L1 L3 L3 LJ
62. 40 50 l 2012 70751 2012 4 12 47 l 10 20 30 40
63. 10 20 30 40 50 2012 70751 2012 4 12 92 IUD 10 l l ID 20 000 30 l 40 50 l
64. jiupgulllllhlli C 00000 jiunpgullluluul li 10 20 30 40 50 2012 70751 2012 4 12 71 I D D H l ID DU l DU l l 10000 l 100000000 0 10 l H 10000 10000 1000 20 0000000000000 l 000 ID IOD 000000
65. 30 40 50 20 2012 70751 2012 4 12
66. C C 40 C 50 C C C C 2012 70751 2012 4 12 44 IU 0000 M Q un 10 l H l 20 l 000 10000 30
67. 00000 DOAGKSPAGVRYHCGDEIILOGFHWNVVREAPYNWYNILROOASTIAADGFSATWMPVPWRDFSSW TDOGK SGGGEGY FWHDFNKNGRY GSDAQLRQAAGALGGAGVKVLYDVVPNHMNEF POKEINLPAG QRFWRNDCPDPGNY PNDCDDGDRFLGGEADLNTGHPQT YGMF RDEF TNLRSGYGAGGFRFDFVRGY APERVDSWMSDSADSSFCVGELWKEPSEYPSWDWRNTASWOOIIKDWSDRAKCPVEDFALKERMON CSVADWKHGLNGNPDPRWREVAVTF VDNHDTGY SPGONGGOHLWALODGLIPOAYAYILTSPGTPV VYWPHMYDWGYGDFIRQLIQVRRTAGVRADSAISFHSGYSGLVATVSGSQQTLVVALNSDLANPGO VASGSFSEAVNASNGOVRVWRSGSGDGGGNDGG ECE DB EID I 103 JP 2012 70751 A 2012 4 12 B TIUS DQAGKSPACVRYHGGDEIILQGFHWNVVREAPYNWYNILRQOASTIAADGESAIWMPVPWRDFSSW TDGGKSCGGEGYFWHDFNKNGRYGSDAQLRQAAGATGGAGVKVLYDVVPNHMNRFY PDKEINLPAG ORFWRNDCPDPGNY PNDCDDGDRF LGGEADLNTGHPOLYGMFRDEFTNLRSGYGAGGFRFDFVRGY APERVDSWMSDSADSSFCVGELWKEPSEYPSWDWRNTA SWQOIIKDWSDRAKCPVFDFALKERMQN GSVADWKHGLNGNPDPRWREVAVTEVDNHDTGYS PGONGGOHLWALQDGLIRQAYAYILTSPGTPV VYWPHMYDWGYGDF TROLIQVREITAGVRADSAI SFHSGY SGLVATV SGSQOTLVVALNSDLANPGQ VASGSFSEAVNASNGQVRVWRSGSGDGGGNDGG 2012070751000001
68. 10 20 30 40 50 12 2012 70751 2012 4 12 10 20 30 40 50 2012 70751 2012 4 12 13 10 20 30 40 50 0
69. 0 0 0 2012 70751 2012 4 12 14 10 20 30 40 50 0 0 0 15 2012 70751 2012 4 12
70. IU l 50 2012 70751 2012 4 12 64 10 20 30 40
71. 2012 70751 2012 4 12 39 10 20 30 C C C 40 l l 50 C 2012 70751 2012 4 12 40
72. 30 40 50 l 2012 70751 2012 4 12 62 10 20 30
73. 30 BSH D R VE 40 10000000000 50 x ID 0010 2012 70751 2012 4 12 72 10 C 20 10000 I D D 30
74. QQAATIAADG ALGGAGVEVL ADLNTGHPQV WKAPSEYPNW RWREVAVTFV YGEFIROLIO SFSEAVNASN SPAAALRLTD ATTVGRL FSATWMPVPW YDVVPNHMNR YGMFRDEFTN DWRNTASWOO DNHDTGYSPG VRRAAGVRAD GOVRVWRSGT TSGYPTWKGS 0000000 10 20 30 40 50 98 2012 70751 2012 4 12 rr Ooo Ooo OOO Ooo Ooo Ooo Ooo OOO Ooo Ooo Oo rrr rrr Oo rr oO oA Lr Di Oooo MSHILRAAVL AAMLLPLPSM ROOAATIAAD GFSAIWMPVP SALGGAGVKV LYDVVPNHMN DADLNTGHPQ VYGMFRDEFT LWKGPSEYPN WDWRNTASWO PRWREVAV PF VDNHDTGYSP GYGDFIROLI QVRRAAGVRA GSFSEAVNAS NGQVRVWRSG WSPAAALRLT DTSGYPTWKG GATTVGRL ADOAGKSPNA WRDFSSWSDG RGYPDKEINL NLRSQYGAGG QIIKDWSDRA GONGGQHHWA DSAISFHSGY TGSGGGEPGA SIALPAGONE VRYHGGDEI SKSGGGEGY PAGOGFWRND FRFDFVRGYA KCPVFDFALK LODGLIRQAY SGLVATVSGS LVSVSFRCDN EWKCLIRNEA NATOVRQWOG I LOGFHWNVVR WHDFNKNGRY CADPGNYPND PERVNSWMTD ERMONGSIAD AYILTSPGTP QOTLVVALNS GATOMGDSVY E EAPNDWYNIL GSDAQLRQAA CDDGDRFIGG SADNSFCVGE WKHGLNGNPD VVYWSHMYDW DLGNP
75. BU l 20 JU DU n 30 DU l 40 0 50 2012 70751 2012
76. C C 40 50 11 2012 70751 2012 4 12
77. 000 DOAGKSPNAV RYHGGDEIIL QGFHWNVVRE APYNWYNILR RDFSSWSDPS RSGGGEGYFW DYPDKEINLP AGORFWRNDC LRSQYGAGGF REDFVRGYAP TIKDWSDRAK CPVFDFALKE ONGGOHIWAL QDGLIRQAYA SAISFHSGYS GLVATVSGSQ GSGGGEPGAL VSVSFRCDNG IALPAGQNEE WKCLIRNEAN 0000000 HDFNENGRYG SDAQLRQAAS PDPGNYPNDC DDGDRFLGGD ERVNSWMTDS ADNSFCVGEL RMONGSIADW KHGLNGNPDP YILTSPGTEV VYWPHMYDWG QTLVVALNSD LGNPGQVASG ATQMGDSVYA VGNVSQLGNW QOAATIAADG FSAIWMPVPW ALGGAGVKVL YDVVPNHMNR ADLNTGHPOV YGMFRDEFTN WKAPSEYPNW DWRNTASWOO RWREVAVTFV DNHDTGYSPG YGEFIRQLIQ VRRAAGVRAD SFSEAVNASN GOVRVWRSGT SPAAALRLTD TSGYPTWKGS ATQVROWOGG ANNSLTPSEG ATTVGRL 000 OOo OOo Oooo DQAGKSENAV RDFSSWSDPS DYPDKEINLP LRSQYGAGGE IIKDWSDRAK QNGGQHEWAL SAISFHSGYS GSGGGEPGAL IALPAGONEE OHUUGUO RYEGGDETIL RSGGGEGYFW AGQRFWRNDC RFDFVRGYAP CEVFDFALKE QDGLIROAYA GLVATVSGSQ VSVSFRCDNG WKCLIRNEAN 0000 QGFHWNVVRE HDFNKNSRYG PDPGNY PNDC ERVNSWMTDS RMONGSIADW YILTSPGTPV QTLVVALNSD ATOMGDSVYA ATOVROWOGG 00000 APYNWYNILR SDAQLROAAS DDGDRFLGGD ADNSFCVGEL KHGLNGNPDP VYWPHMYDWG LGNPGQVASG VGNVSOLGNW ANNSLTPSEG
78. 10 20 30 40 50 2012 70751 2012 4 12 7 10 IOD 20 30 40 50 2012 70751 2012 4 12 8 10 20 30 40
79. gt Du H Ou DO0000000 00000000 uu H H 1 00000000000 CHU Geo C C C 10 00000000000 oar 0 000 000 10u z 5 C 0 _ 00000000000 H 1000000000000 E pin ug C 00000 5 woo 000 VA VR D GR M 10 20 30 40 50 68 2012 70751 2012 4 12 uuu N33Y GCGAAGCGCCCTACAACTGGTACAAC MSDM D34N A179T G223A GGCGAGCTGTGGAA AgecCCTTCIGAATATCCG H307L gaacGGCGGCCAGCACctgTGGGCGCTGCAG MSDM S334P GTACTGGeceCACATGTACGACTGGGGCTACGGC MSDM D343E gaaTTCATC a EUER ET HE QAR SCE DZ Oo GCGAAGCGCCCTACAACTGGTACAAC D34N CCGACGGCGGCAGGTCCGGCG G121D CACATGAACCGCGACTACCCGGACAAG 1 MSDM G134R CTGCCGGCCGGCCAGcGCTTCTGGCG MSDM A141P CGCAACGACTGCGCCGACCCGGG M
80. 10 104 2012 70751 2012 4 12 5 Irt d 1000000000 0000 230 2006 01 0000 1 30 10000 0000 226 2006 01 0000 226 10000 0000 1700 2006 01 1000 1300 0000 vos 2006 01 1000 vos 0000 16 2006 01 1000 1 16 31 10 886 527 32 18 2004 7 7 33 5 31 60 612 407 32 16 9 220 2004 9 22 33 5 31 10 947 612 32 16 9 220 200
81. 2012 70751 2012 4 12 19 JP 192 Bg Fe ET 4 FRA 55 2012 70751 P2012 70751A 43 32824545125 2012 4 12 51 Int Cl FI BF C12N 15 09 2006 01 C12N ZNAA 2B150 C12N 9 26 2006 01 C12N 4B018 C12N 1 15 2006 01 C12N 4BO24 C12N 1 19 2006 01 C12N 4B032 C12N 1 21 2006 01 C12N 4B035 70 OL 106 E 2 ERES 402011 258176 P2011 258176 71 397060588 22 3E 23411 825 2011 11 25 IL LA 62 FORA 15 582007 519884 P2007 519884 2 Fh F DAI 4774 1001 T 2 RARA 3ERETT E7R 2005 7 7 17 1 51 AAEE 60 608 919 71 595163630 32 48 3ERK16fE7 R T 2004 7 7 MLA 4YAI RU4 FY 33 BERD US F 81 FAEERE 10 886 504 Danisco US Inc 32 48 3Ep amp 164 E7R7R 2004 7 7 7 V36RE94304 1013 gt 93 US V731 7MWt0 31 ci XESR EE 10 886 505 01 92 52 32 32 16 2004 7 7 74 KFBA 100078282 33 EI XE 8 US Ft BR TREE lt 54 7 F 5 0000
82. 10 20 30 40 50 81 2012 70751 2012 4 12 IU U 0 0000000000000 00000000 00000000 000000000 0 0 0 ETERO
83. aatcccggcc gtgegegtgt gtgagtttcec aacgtcagcc ggctacccga tgcctgatcc aacagcctga ctcggcegtc eggggccgec ttgccagcga LI rr Ooo 0O rrr Ooo 0O Ooo Ooo rrr rrr rrr Ooo 0O Ooo Ooo Ooo Ooo Ooo Ooo 0O rrr L3 Lr rr OOO OOO rr Lr rr 10 20 30 40 99 JP 2012 70751 A 2012 4 12 00000 1 DOAGKSPAGV RYHGGDEIIL OGFHWNVVRE APYNWYNILR QQASTIAADG FSAIWMPVPW 61 RDFSSWTDPG KSGGGEGYFW HDFNKNGRYG SDAQLRQAAG ALGGAGVKVL YDVVENHMNR 121 FYPDKEINLP AGQRFWRNDC PDPGNYPNDC DDGDRFLGGE SDLNTGHPQI YGMFRDEFTN 181 LRSGYGAGGF RFDFVRGYAP ERVDSWMSDS ADSSFCVGEL WKAPSEYPSW DWRNTASWOO 241 IIKDWSDRAK CPVFDFALKE RMONGSVADW KHGLNGNPDP RWREVAVTFV DNHDTGYSPG 301 ONGGOHIWAL QDGLIRQAYA YILTSPGTPV VYWPHMYDWG YGDFIRQLIO VRRTAGVRAD 361 SAISFHSGYS GLVATVSGSQ QTLVVALNSD LANPGQVASG SFSEAVNASN GOVRVWRSGS 421 GDGGGNDGG DUE Bop T DE LEES GG EP ECT GEI EIE ETE VV ALTI E EE EHE EI SEEMS EE a ELE EE A ER BITES
84. DBLULuulu iu ubiudgdgdiultluultuuiuulllLullLuLliutluulu 10 r1 rt 000000000 DO 20 DO A DOO 30 5 GAA TTC AGC CGC CAT C 5 AGA TGT TTC GC 40 2R 5 TAG CCG AAG AT 5 TGA CCT TCG TCG ACC AC 5 GAT AGC TGC TGG TGA CGG TC 1000000 10000000060 L1 66 2012 70751 2012 4 12 uu G134R G134R ogccag 11571 ee eget XGGCGGCGAGTOG HSIL cgactcgccg 62234 G223
85. 20 30 40 50 2012 70751 2012 4 12 61 10 20 DU l DI l
86. 2012 70751 2012 4 12 58 10 20 30 40 50 2012 70751 2012 4 12 59 10 20 30 Ooo L3 O O L L3 O Oo L3 L3 O
87. EI DOAGKSPAGVRYHGGDEITILOGFHWNVVREAPYNWYNILRQQASTIAADGFSAIWMPVPWRDFSSW TDGGKSCGGEGYFWHDFNKNGRYGSDAQLRQAAGALGGAGVKVLYDVVPNHMNREYPDKEINLPAG ORFWRNDCPDPGNYPNDCDDGDRFLZIGEADLNTGHPQIYGMFRDEFTNLRSGYGACCFRFDEVRGY APERVDSWMSDSADSSFCVGELWKEP SEY PSWDWRNTASWQQ I TRDWSDRAKC PVF DFALKERMON GSVADWKHGLNGNPDPRWREVAVTFVDNHDTGYSPGONGGQHLWALODGLIRQAYAYILTSPGTPV VYWPEMYDWGYGDFIROLT QVRRTAGVRADSAI SFHSGY SGLVATVSGSQQTLVVALNSDLANPGQ VASGSFSEAVNASNGOVRVWRSGSGDGGGNDGG 0000 0000 0000 m rrr rrr Cir EJ 5 DQAGKSPAGVRYEGGDEIILQGFHWNVVREAPYNWYNILRQQOASTIAADGFSAIWMPVPWRDE SSW TDGGKSGGGEGYFWHDFNKNGRYGSDAQLRQAAGALGGAGVREVLYDVVPNHMNRFYPDKEINLPAG ORFWRNDCPDPGNYPNDCDDGDRFLOGEADLNTGHPQIYGMFRDEFTNLRSGYGAGGFRFDEVRGW APERVDSWMSDSADSSFCVGELWKEPSEYPSWDWRNTASWOOIIKDWSDRAKCPVFDFALKERMON CSVADWKHGLNGNPDPRWREVAVTFVDNHDTGYSPGONGGOHLWALQDGLIRQAYAYILTSPGTPV VYWPHMYDWGYGDFIRQLIQVRRTAGVRADSAISFHSGYSGLVATVSGSQOTLVVALNSDLANPGQ VASGSFSEAVNASNGQVRVWRSGSGDGGGNDGG
88. C 10000 10 20 30 40 50 2012 70751 2012 4 12 5 10 D l 20 30 40 50
89. 2012 70751 2012 4 12 28 10 20 30 40 DU DU l 50 2012 70751 2012 4 12 29 10 20 30
90. Oo L3 Oo L3 Ooo O O Ooo apes O O Ooo 40 l l 50 l 2012 70751 2012 4 12 60 10 C 000 C ID 000
91. 2012 4 12 69 100000 10 20 n H n 30 40 DU l 50 70 2012 70751 2012 4 12
92. 4 12 52 DU l 10 20 30 40 50 l 2012 70751 2012 4 12 53 10 20
93. gacttctcca gacttcaaca ctcggtggcg tatecggaca gacecaggca gacctcaaca cgcagtcagt egggtcaaca aaaggcccct gacttcatcc eatcagcttcc ctggtggtgag agcgaggcgg ggtggcggtg cagatgggcg geegeggcgt ttgectgecg acegteggec gtcteggcgg ctagtttteg gtacaggteg ctcaccagcc gggtttggta cgaagatgtg ttgccgocag agagccccaa ggaacgtcgt ccatcgccgc gctggagcga agaacggeeg ccggegtgaa aggagatcaa actaccccaa ceggccaccc acgatgccgg gctggatgac ctgagtacec atcaaggact ggtcegaccg ctcgatogcc cgcgaggtgg cggtgacctt ggtgggcagc accactgggc ccggtacgcc gatcaggagc gaactgctgc ctacccgaac cgctatgcgc ccgcgaagcg cgacggcttc Cggcagcaag ctatggcagt agtgctttac tgattgcgac ccaggtctac eggettccga cgatagcgcc gaactgggac ggccaagtgc gactggaagc cgtcgacaac tctgcaggac ggtggtgtac gtcagetgat acagcggcta cgctcaactc tcaacgccag aacccggcgc acagcgtcta gccagaacga aatggcaggg ggctctagcc eggtategtt gcgctccgcqd tcgagcagecg ccaggtgegt cagceggtctg egacctgggc caacggccag tetggtcagt egeggtegge ggaatggaaa cgaggcaaac cgggcggcaa gcgctaggog catcggccag ct gccgagagca tggccgagat aagcacaaga taccacggcg cccaacgact tcggcgatct tccggcggcg gacgtggtge ggccagggct gacggcgacc ggcatgttcc ttcgactttg gacaacagct ccggtgtteg acgectgaac cacgacaccg gggetgatce tggtegcaca egegeegecg
94. 0000000 1 1 1 1 YP ft i tt ee ee ee ee 20 gatcggcgta ggaagcaggc ggaaatcgac ttaggacgaa tgccgaatcg gaggatggct cgtaatcagg tgattgccag gtggtcggogg cctcgtcogg tgagccacat cegatcaggc tccagggctt gecaacaggc ggcgtgactt ggcacgactt gcgcactcgg gcggctaccc gcgccgaccc agtcggacct acctgcgcag ccgagcgggt tgtggaaagg agatcatcaa agcgcatgca cgcgctggcg ggcagaacgg cctacatcct gctacggcga attcagcgat agcagaccct gcagcttcag gcggcgatgg gcgacaacgg tcggcaactg ggaagggcag acgaggcgga ccgccgccgg cgcctacgcc agctgttcat tgtacctgct ttgccatcgg tggtcagcga tagccgagga accaggccct agcaagcctg gcacgcgacc ttctcgcggac gcctattcca ccatcggcgt ggtttcgcat ctggtcgccg cgccagggcc cagggggtgc atcacgcctt ccgctgagcgg atgagtgcgg gggccttcgg cgtgcaggag Ccggccttgcc cctgegtgcc cggcaagagc ccactggaac ctcgacgatc ctccagctgag caacaagaac tggcgccggg ggacaaggag gggcaactac gaacaccggc cggctacggc cgacagctgg cccttctgaa ggactggtcc gaacggctcg cgaggtggeg cggccagcac caccagcccg cttcatccgc cagcttccat gatggtggcg cgaggcggtc cggcaggaat cgtgacgcag gagcceggcc categecctg cgcgacgctg cacgagcacc gogcggctcc atgctggcac gttggcccag gatcgccgca cegectgcaa gagcttcttc ecggectgac cgagctgttc gcggcgctgt tgctcgcgcg tcaagcagga tcaagcagtt gtcttcgatc tcgttgccca gggccgccga gcggtgtgct egetgegtgt catectcccg c
95. 12 RAE fk bas pMD153 206 N33Y D34N G121F G134R A141P Y146G 1157L G158T S161A L178F A179T G223E 8229 H307L A309P 334 N33Y 0343 G70D G121F G134R A141P Y146G 11571 G158T S161A L178F A179T G223E S229P H307L 100000 A309P 334P bas pMD153 12 3 206 N33Y D34N G121F G134R A141P Y146G 11571 G158T S161A L178F SAS1387 D16 bf 22 3 A179T G223E 5229 H307L A309P 5334 HOO 000000000 N33Y D34N G121F G134R A141P N145D Y146G 11571 G158T 5161A L178F A179T G223E 229P H307L A309P 5334 10 20 30 40 85 JP 2012 70751 A 2012 4 12 0485 _ ER bas N33Y D34N G70D G121F G134R A141P N145D Y146G 1157L G158T S161A L178F A179T G223E S229P
96. H307L A309P 5334 W339E pMD236 wem w N33Y D34N G70D G121F G134R A141P N145D Y146G I157L G158T S161A L178F A179T G188H G223E S229P H307L A309P S334P W339E N33Y D34N G70D G121F G134R A141P N145D Y146G 11571 G158T S161A L178F A179T G223E S229P H307L A309P S334P W339E N33Y D34N G70D G121F G134R A141P N145D Y146G 11571 G158T S161A L178F A179T G188S G223E 5229 H307L A309P 5334 W339E 10 20 30 40 86 2012 70751 2012 4 12 9 85 Betamyl Phadei bas 2 206 N33Y D34N G121F 01348 141 Y146G 11571 G158T S161A L178F A179T G223E S229P H307L A309P hy SAS1379 013 11571 G158T S161A L178F A179T G223E S229P H307L A309P 8334P W339A
97. 0 30 40 50 2012 70751 2012 4 12 10 10 20 30 C C C
98. 1157L L178F A179T G223K H307L S334P 000 000 nannan 10 U BOM EE EE gm 000 D nooo bas 1157L L178F A179T H307L S334P D34N G121D G134R A141P 1157L L178F A179T G223V H307L S334P 000 DO0O000O0O00O0O0OO0O00000000000 00 0000000000000000 0000000000000 00000000 00000000 0000000 30 bas I157L L178F A179T H307L S334P 3 0 D34N G121D G134R A141P 1157L L178F A179T G223E H307L S334P 40 L Oo
99. 34R A141 P 1157L L178F A179T G223A H307L 8334P 5 1 115 N33Y D34N G121F G134R A141P 1157L L178F A179T G223E H307L 334P bas L178F 179 G223A H307L 5334 N33Y D34N G134R A141P I157L L178F A179T G121D H307L S334P 90000 100110011000 100000100919101 H HUU 77 2012 70751 2012 4 12 ID DI 1 DI DI DI DI DI DI DI DI DI DI DI DI DI oana D t 80 Betamyl Phade bas
100. 4 9 22 33 5 74 100062409 74 100113413 72 00 0000 0000000 00 10000 0000 72 0000 0000 00000 0000 72 000000 00000 100 000000000 72000 10000 000000 10000000 0 79000 H JUD 0 000 _ 0000 000 10 000 000000 70000 0000 _ 0000 0000 10000 0 79000 H 100000 00 00 100 00000 000 _ 72000 100000 00000 000 00000 7 00 0000 000000600 0 1000 00 7200 0000 000 28150 D23 4B018 LBOL MB4 M02 M12 48024 A05 2 3 BA77 CA01 9 Q0 0412 Gil 48032 815 4B035 1005 161 48050 494 GD HL 10 0002 HALL 108 POS LP21 LLO2 105 2012 70751 2012 4 12 48065 AA01X AMIX AMIY AA6OX ABOl ACI4 0951 052 COMI G42 106 2012 70751 2012 4 12 2012070751000001 pdf
101. 751 2012 4 12 31 l IU 000 ID DU l ID 10 DU l n 000000 20 30 40 l n 50 l l MSHILRAAVLAAVLLPFPALA
102. A p anggege HOO sas H307L etg pigcte sccgccg tt 5334 GTACTGGcogCACATGTACGACTGGGGCTACGGC D343E gaat TCATC gatgaattogcogtagececagtogtacatgtecggecagtac D343E Klenow Fsel gt mta C8037L 477h CSA2 J v DNAJ mta o Fspl Pstl 2577 7 72 DNAJ mta oPstl Aspl 7277 Ot 100000 H 00000 00000 000000 0 tuu ug 0000600 0 000000000000000000000000000000000 0 00000000 100000 00000600
103. ANPGOVASG SFSEAVNASN GDGGGNDGG 0000 FSAIWMPVPW YDVVPNHMNR YGMFRDEFIN DWRNTASWQO DNHDTGYSPG VRRTAGVRAD GOVRVWRSGS ngaggupnpngagggagaguupnpnagagguuniu e u 10 20 30 40 95 2012 70751 2012 4 12 RAM ELSE TO UD TEM 0 00000 MSHILRAAVL AAVLLPFPAL ADDAGKSPAG VRYHGGDEII LOGFHWNVVR EAPNDWYNIL ROQASTIAAD GFSAIWMPVP WRDFSSWTDG GKSGGGEGYF WHDFNKNGRY GSDAQLRQAA GALGGAGVKV LYDVVPNHMN RGYPDKEINL PAGOGFWEND CADPGNYPND CDDGDRFIGG ESDLNTGHPO IYGMFRDELA NLRSGYGAGG FRFDFVRGYA PERVDSWMSD SADSSFCVGE LWEGPSEYPS WDWRNTASWO QIIKDWSDRA KCPVFDFALK ERMONGSVAD WKHGLNGNPD 10 PRWREVAVTF VDNHDTGYSP GQNGGOHHWA LQDGLIRQAY AYILTSPGTP VVYWSHMYDW GYGDFIRQLI QVRRTAGVRA DSAISFHSGY SGLVATVSGS QQTLVVALNS DLANPGOVAS GSFSFAVNAS NGQVRVWRSG SGDGGGNDGG EGGLVNVNFR CDNGVTOMGD SVYAVGNVSO LGNWSPASAV RLTDTSSYPT WKGSIALPDG ONVEWKCLIR NEADATLVRO WOSGGNNOVO AAAGASTSGS F
104. EI 00000000000 0000000 E DE T 00000 DOAGKSPAGVRYEGGDEIILOGFHWNVVREAPYNWYNILROQASTIAADGFSAIWMPVPWRDFSSW TDGGKSOGGEGYFWHDFNKNGRYGSDAQLRQAAGALGGAGVKVLYDVVPNHMNRFYPDKEINLPAG QRFWRNDCPDPGNYPNDCDDGDRFLGGEADLNTGHPQIYCMFRDEFTNLRSCYOGAGGFRFDFVRGY APERVDSWMSDSADSSFCVGELWKEPSEYPSWDWRNTASWOQIIKDWSDRAKCPVEDFALKERMQN GSVADWRHGLNGNPDPRWREVAVTFVDNHDTGYSPOQNGGOHLWALQDGLIROAYAYILTSPGTPV VYWPHMYDWGYGDFIRQLIQVRRTAGVRADSAISFHSGYSGLVATVSGSOQTLVVALNSDLANPGQ VASGSFSEAVNASNGOVRVWRSGSGDGGGNDGG 00000 DOAGKSPAGVRYHGGDEIILQGFHWNVVREAPYNWYNILROQASTIAADGFSAIWMPVPWRDFSSW TDGCKSCGCEGCYFWHDFNKNGRYGSDAQLRQAAGALGGAGVEVLYDVVPNHMNRFYPDKEINLPAG ORFWRNDCPDPGNGPNDCDDGDRF LGGEADLNTGHPQI YGMFRDEF TNLRSGY GAGGFRFDFVRGY APERVDSWMSDSADSSFCVGELWKEPSEYPSWDWRNTASWOQIIKDWSDRAKCPVFDFALKERMON GSVADVKEGLNGNPDPRWREVAVTFVDNHDTGYSPGONGGOQHLWALODGLIROAYAYILTSPGTPV VY
105. F A179T G223A H307L D343E S334P PSac D20 7 1 2 7 N33Y D34N K71R 61210 G134R RIS 3 e 1157L L178F A179T G223A H307L D343E S334P PSac D34 8 4 67 N33Y D34N G121D G134R A141P 2 14571 L178F 179 G223A H307L 5334P RABS 13 L178F A179T G223A H307L S334P pMD55 54 N33Y D34N G121F G134R A141P 1457L G223A H307L S334P L178F 179 pMD85 5 1 115 IN33Y D34N G121F G134R A141P 11571 G223E H307L S334P L178F A179T PMD96 4 0 231 N33Y D34N G121F G134R A141P 11571 G223E H307L S334P L178F A179T 5161 1157L G223E H307L S334P L178F A179T N33Y D34N G121A G134R A141P 11571 G223E H307L S334P L178F A179T S161A O PROLE D D D D D 0 1100 i
106. FHWNVVREAPYNWYNILRQQOASTIAADGFSAIWMPVPWRDESSW TDCCKSGGGEGYFWHDFNKNGRYGSDAQLRQAAGALOGAGVKVLYDVVPNHMNREYPUKEINLPAG ORFWRNDCPDPGNY PNDCDDGDRFLGGEADLNTGHPQI YGMF RDEF TNLRSGYGAGGFRFDFVRGY APERVDSWMSDSADSSFCVGELWKEPSEYPSWDWRNTASWOOIIKDWSDRAKCPVFDFALKERMQN GSVADWKHGLNGNPDPRWREVAVTFVDNEDTGY SPGONDGQHLWALQDGLIRQAYAYILTSPGTPV VYWPHMYDWGYGDFIRQLIQVRRTAGVRADSAISFHSGYSGLVATVSGSOOTLVVALNSDLANPGQ VASGSFSEAVNASNGQVRVWRSGSGDGGGNDGG rr 20 Iz Ooo 0O Ooo rrr rrr Ooo OOO OOO OOO O OOO O OOO OOo DOAGKSPAGVRYHGGDEIILQGFHWNVVREAPYNWYNILRQQOASTIAADGFSAIWMPVEWRDFSSW TDGGKSGGGECYFWHDFNKNGRYGSDAQLURQAAGALGGAGVKVLYDVVPNHMNREYPDKEINLPAG ORFWRNDCPDPGNYPNDCDDGDRFLGGEADLNTGHPQIYOMFRDEPTNLRSGYGAGGFRFDEVRGY APERVDSWMSDSADSSFCVCELWKEPSEYPSWDWRNTASWOOIIKDWSDRAKCPVFDFALKERMQN GSVADWKHGLNGNPDPRWREVAVTFVONHDTGY SPGONGGOTLWALODGLIROAYAY ILTSPGTPV VYWPHMYDWGYGDFIRQLIOVRRTAGVRADSAISFHSGYSCLVATVSGSQQTLVVALNSDLANPGO VASGSFSEAVNASNGOVRVWR
107. GQVAS AVGNVSQLGN GANNSLTPSE O a ET DI ES B EH T Lr zr Ooo 0O rrr 1 gatcggcctt tacggaaagt gatagagctt ctcttceggc aaactttgtt ccccagtgac 61 121 181 241 301 361 421 481 541 601 661 721 7 8 81 21 901 9 1021 1081 1141 1201 1261 1 1381 1441 1501 1561 1621 1681 1741 1801 1861 1921 1981 2041 61 agagggttag ggatgaaatc ccggccggcg accggagtat egttgecgte gcgacgaaat ggatgccggt gtgaadgcta tgcgtcaggac ccaaccacat tetggegcaa gettcatcgg gegatgaatt ttoggggeta tctgcgtodg acttcgcoct gctactcgcc ccgccagctg ggcaateceg gecaggccta tatcggatcg ctgcggecagd ttcatcctog tgcgatgagc catggccgat cattctccag ectgcgccag geectggcge cttctggcac gaaccgtggc cgactgcgcc cggcgatgcg caccaacctg tgcgccggag cgaactgtgag gcagcagatc caaggaacgc acccgcgtgg Cgggcageac egectacatc 321 tgtacgactg gegtgegege tcagcggcag aggtggccag ggcgtagcgg gctgcgacaa agctcggtaa cctggaaggg gcaacgaggc egecgagcga tegeggatgt gttcacgege atcgectgcg gggttacgge cgattcggcg ccagcagacc cggcagcttc cacgggcagc cggegegacg ctggagcccg cagcattgcc caacgccacc gggcgccacc gaggcggctg cctgctatog etteggectg cttcctcttt aaggtcgcgc tccggeggcc cacatectge caggccggca ggctttcact caggccgcga
108. GYAP ERVNSWMTDS ADNSFCVGEL CPVFDFALKE RMONGSIADW KHGLNGNEDP QDGLIRQAYA YILTSPGTPV VYWSHMYDWG GLVATVSGSQ QTLVVALNSD LGNPGOVASG VSVSFRCDNG ATOMGDSVYA VGNVSQLGNW WKCLIRNEAN ATOVROWOGG ANNSLTPSEG JP 2012 70751 A 2012 4 12 FSAIWMPVPW YDVVPNHMNR YGMFRDEFTN QOAATIAADG ALGGAGVKVL ADLNTGHPOV WKGPSEYPNW DWRNTASWOO RWREVAVTFV DNHDTGYSPG YGDFIRQLIQ VRRAAGVRAD SFSEAVNASN GQVRVWRSGT SPAAALRLTD TSGYPTWKGS ATTVGRL 000 ELI OOO 121 DQAGKS PNAV RDFSSWSDPS DYPDKEINLP RYHGGDETIL KSGGGEGYFW AGORFWRNDC QGFHWNVVRE APYNWYNILR QOAATIAADG HDFNKNGRYG SDAQLROAAS ALGGAGVKVL PDPGNYPNDC DDGDRFLGGD ADLNTGHPQV FSAIWMPVPW YDVVPNHMNR YGMFRDEFTN 181 241 301 361 421 481 RFDFVRGYAP CPVFDFALKE QDGLIRQAYA GLVATVSGSQ VSVSFRCDNG WKCLIRNEAN LRSQYGAGGF IIKDWSDRAK QNGGOHIWAL SAISFHSGYS GSGGGEPGAL TALPAGQNEE 0000000 ERVNSWMTDS ADNSFCVGEL WKAPSEYPNW RMONGSIADW KHGLNGNPDP RWREVAVTFV YILTSPGTPV VYWPHMYDWG YGDFIROLIO QTLVVALNSD LGNPGQVASG SFSEAVNASN ATQMGDSVYA VGNVSQLGNW SPAAALRLTD ATQVRQWQGG ANNSLTPSEG ATTVGRL DWRNTASWOQ DNHDTGYSPG VRRAAGVRAD GOVRVWRSGT TSGYPTWKGS
109. LANPGOVASG QOASTIAADG ALGGAGVKVL SDLNTGHPQI WKAPSEYPSW RWREVAVTFV YGDFIRQLIO SPSEAVNASN FSATWMPVPW YDVVPNHMNR YGMFRDEFTN DWRNTASWQO DNHDTGYSPG VRRTAGVRAD GQVRVWRSGS 0000 Lr Ooo 000 121 181 241 301 361 421 DOAGKSPAGV RYHGGDEIIL QGFHWNVVRE APYNWYNILR OQASTIAADG FSAIWMPVPW RDFSSWIDPG RSGGGEGYFW HDFNKNGRYG SDAOLROAAG ALGGAGVKVL YDVVPNHMNR DYPDKEINLP AGQRFWRNDC PDPGNYPNDC DDGDORFLGGE SDLNTGHPQI YGMFRDEFIN LRSGYGAGGF RFDFVRGYAP ERVDSWMSDS ADSSFCVGEL WKAPSEYPSW DWRNTASWQO IIKDWSDRAK CPVFDFALKE RMONGSVADW KHGLNGNPDP RWREVAVTFV DNHDTGYSPG QNGGOHLWA ODGLIROAYA YILTSPGTPV VYWPHMYDWG YGEFIRQLIO VRRTAGVRAD SAISFHSGYS GLVATVSGSO QTLVVALNSD LANPGOVASG SFSEAVNASN GOVRVWRSGS GDGGGNDGG 000 00 00000 0000 Ooo 000 121 241 301 361 421 DQAGKSPAGV RYHGGDEIIL QGFHWNVVRE APYNWYNILR QOASTIAADG RDFSSWTDPG RSGGGEGYFW HDFNKNSRYG SDAOLROAAG ALGGAGVKVL DYPDKEINLP AGQRFWRNDC PDPGNYPNDC DDGDRFIGGE SDLNTGHPQI LRSGYGAGGF RFDFVRGYAP ERVDSWMSDS ADSSFCVGEL WKAPSEYPSW IIKDWSDRAK CPVFDFALKE RMONGSVADW KHGLNGNPDP RWREVAVTFV QNGGOHLWAL QDGLIRQAYA YILTSPGTPV VYWPHMYDWG YGEFIRQLIQ SAISFHSGYS GLVATVSGSO QTLVVALNSD L
110. QRFWRNDCPDPGNY PNDCDDGDRFLGGEADLNTGHPQT GMFRDEF TNLRSGYGAGGFRFDFVRGY APERVDSWMSDSADS SFCVGELWKEP SEY PSWDWRNTASWQQT LKDWSDRAKCPVFDFALKERMQON GSVADWKHGLNGNPDPRWREVAVTF VDNHDTGY SPGQNGGOQHLWPLODGLIRQAYAYILTSPGTPV VYWPHMY DWGYGDF LROLTOVRRTAGVRADSALI SFHSGY SGLVATVSGSQQTLVVALNSDLANPGQ VASGSFSEAVNASNGQVRVWRSGSGDGGGNDGG ee ee a IO DO SOS NO E LOT DI 00000000000 00000000000 D Dg DOACKSPAGVRYHGGDEIILQGFHWNVVREAPYNWYNILROQASTIAADGFSAIWMPVPWRDESSW TDGGKSCOGEGYFWHDFNKNGRYGSDAQURQAAGALGGAGVKVLYDVV PNHMNRF Y PDKEINLPAG QRFWRNDCPDPGNYPNDCDDGDRFLGGEADLNTGHPQIYGMFRDEFTNLRSGYGAGGFRFDFVRGY APERVDSWMSDSADSSFCVGELWKEPSEYPSWDWRNTASWQOIIKDWSDRAKCPVFDFALKERMON CSVADWRHGLNGNPDPRWREVAVTFVDNHDTGYSPGQNGGOHLWALODGLISQAYAYILTSPGTPV VYWPHMYDWGYGDFIRQLIQVRRTAGVRADSAISFHSGYSGLVATVSGSOQTLVVALNSDLANPCQ VASGSFSEAVNASNGOVRVWRSGSGDGGGNDGG Yt 000000000000000000000 00
111. RMON GSVADWKHGLNGNPDPRWREVAVTFVDNEDTGYSPGONGGOHLWALODGLIRQAYAYILTSPETPV VYWPEMYDWGYGDFIRQLIQVRRTAGVRADSAISFHSGYSGLVATVSGSQQOTLVVALNSDLANPGQ VASGSFSEAVNASNGOVRVWRSGSGDGGGNDGG 101 JP 2012 70751 A 2012 4 12 Ooo Ooo OOO OOo Ooo Oooo Oooo Oo Oooo OOOO Oooo Oo DQAGKSPAGVRYHGGDEIILQGFHWNVVREAPYNWYNILRQOASTIAADGFSAIWMPVPWRDFSSW TDGGKSCGGEGYFWHDFNKNGRYGSDAQLRQAAGADGGAGVKVLYDVVPNHMNRFYPDREINLPAG QRFWRNDCPDPGNYPNDCDDGDRFLGGEADLNTGHPQIYGMFRDEFTNLRSGYGAGGFRFDPEVRGY APERVDSWMSDSADS SFCVGELWKEP SEY P SWOWRNTASWQQ I I KDWSDRAKC PVF DFALKERMQN GSVADWKHGLNGNPDPRWREVAVTFVDNHDTGYSPGONEGOHLWALODGLIROAYAYILTSPGTPV VYWPHMYDWGYGDFIRQLIQVRRTAGVRADSAISFHSGYSGLVATVSGSQOTLVVALNSDLANPGQ VASGSFSEAVNASNGQVRVWRSGSGDGGGNDGG rr 10 OOO OOO OOO rrr Ooo Ooo L3 L3 OOOO OOOOH OOOOH OOO 000 DOAGKSPAGVRYHOGDEIILOG
112. SDM GACGGTGACCGCTICCTgGGCGGCGAGTCG MSDM L178F CGCGACGAGTITACCAACCTGCG 179 i G223A GGCGAGCTGTGGAAAgccCCTTCIGAATATCCG MSDM gaacGGCGGCCAGCACetg TGGGCGCIGCAG MSDM S334P GTACTGGcegCACATGTACGACTGGGGCITACGGC D343E gaaTICATC N33Y GCGAAGCGCCCTACAACTGGTACAAC MSDM D34N GI21D CACATGAACCGCGACTACCCGGACAAG MSDM GI34R CIGCCGGCCGGCCAGGGCTICIGGCG 15 MSDM A141P CGCAACGACTGCGCCGACCCGGG MSDM 1157L GACGGTGACCGCTTCCTgGGCGGCGAGTCG MSDM L178E CGCGACGAGTTTACCAACCTGCG MSDM A179T G223A GGCGAGCTGTGGAAAgccCCTTCTGAATATCCG MSDM H307L gaacGGCGGCCAGCACcigTGGGCGCIGCAG MSDM GTACTGGeegC ACATGTACGACTGGGGCTACGGC GO O O VD VC Vago VV VO E EE yi EET 00 AGO RD a a Al UD DG MG VO DO Al A ERA SV DI ET 0 0000000000 Pet TOGO Po WP 1000600 2012 70751
113. SGSGDGGGNDGG 40 Ooo Ooo 0O rrr rrr Ooo rrr Ooo 0O Lr OOO AOA OO rr 102 JP 2012 70751 A 2012 4 12 00000 DOAGKSPAGVRYHOGGDEIILQGFHWNVVREAPYNWYNILRQOASTIAADGFSAIWMPVEWRDFSSW TDGCKSGGGECYFWHDFNKNGRYGSDAQLROAAGALGGACVRVLYDVVPNHMNRFYPDKEINLPAG ORFWRNDCPDPGNY PNDCDDGDRF LGGEADLNTGHPQLYGMFRDEFTNLRSGY GAGGFRFDFVRGY APERVDSWMSDSADSSFCVGELWKEPSEY PSWDWRNTASWOQQI IKDWSDRAKC PVF DFALKSRMQN GSVADWKHCLNCNPDPRWREVAVIFVDNEDTGYSPGONGGOGLNALODGLIROAYAYILTSPGTPV VYWPHMYDWGYGDFIROLTOVRRTAGVRADSAISFHSGYSCLVATVSGSQQTLVVALNSDLANPGQ VASGSFSEAVNASNGOVRVWRSGSGDOGGNDGG HH Er 000000 00000 DOAGKSPAGVRYHGGDEIILOGFHWNVVREAPYNWYNILRQOASTIAADGFSAIWMPVPWRDFSSW TDGCKSGGGEGYFWHDENKNGRYGSDAQLRQAAGALGGAGVEVLYDVVPNHMNRFYPDKEINLPAG
114. WPHMYDWGYGDFIROLIQVRRTAGVRADSAISFHSGYSGLVATVSGSQOTLVVALNSDLANPGQO VASGSFSEAVNASNGQVRVWRSGSGDGGGNDGG ED ELI E ER ELE N OD O E DA a EE HI 00000 DQAGKSPAGVRYHGGDEIILOGFHWNVVREAPYNWYNILRQOASTIAADGFSAIWMPVPWRDESSW TDGGKSGGGEGYFWHDFNKNGRYGSDAQLRQAAGALGGAGVKVLYDVVPNHMNREYPDKEINLPAG QRFWRNDCPDPGNYPNDCDDGDRFMGGEADLNTGHPOIYGMFRDEFTNLRSGYGAGGFRFDEVRGY APERVDSWMSDSADSSFCVGELWKEPSEYPSWDWRNTASWOOITIKDWSDRAKCPVFDFALKERMON GSVADWKHGLNGNPDPRWREVAVTFVDNHDTGYSPGQNGGOHLWALODGLIRQAYAYILTSPGTPV VYWPHMYDWGYGDFIRQLIOVRRTAGVRADSAISFHSGYSGLVATVSGSQQTLVVALNSDLANPGQ VASGSFSEAVNASNGQVRVWRSGSGDGGGNDGG ee ee ee 100 JP 2012 70751 A 2012 4 12 O DBDUUDBBBUB UA EL Sgt 0000 0000 1000
115. gtaatccct cctggccact cagectgcgg gtaggatacc gccgtattgg CCggccggogg gtcgtccgcg gcggccegacg accgacggcg ggccgctacg gtgaaggtgc atcaacctgc cccaacgact catccgcaga gccggeggct atgagcgaca tatccgagct gaccgggcca gtcgccgact gtgaccttcg cactgggcgc ggcacgccag cagctgatcc agcggctaca ctcaactccg aacgccagca gacggcggca atgggcgaca tccacggtac ectgacggtc gtgcgtcagt agcggctcgt tcccgaccca gacaaccata acccgctcga ctggtoggcct ggcaatgccg ggtacgttge cagctcgacg gcccagctgg tgcoggagct tggggcgcaa cctgctgcag catcatcccg aaggcactcg 96 ggcgatattt tggcgccgac ccagcagggc tcatgacgag cgcagggcgc accagagatt gaggtggggc acgcegcctg gtoccgatcg Cgaacaagca cggcggtcct tgcgctacca aagcgcccaa gcttctcgogc gcaagtcogg gcagcgacgc tctacgatgt cggccggcca gcgacgacgg tttacggcat tccgcttcga gcgccgacag gggactggcg agtgcccggt ggaagcatgg tcgacaacca tgcaggacgg tggtgtactg aggtgcggcg atctggccaa acggccaggt agggtggctt gcgtctacgc ggctgaccga agaacgtgga ggcaatcggg tetgacgaca gggtgagcag aaagccttcg cccctttcog tgctgatgct agcaaatctc agagcctgac gctatgtcgg agcgggtgac ctcgagcagg atgaagcggg cgccggtgcg gcccgaacgc ggcaagcagg gtecttgttt agetcggtgg tegctggcgc tacgcceggc caactgggcg aagacccggc gctgccgttt cggcggcgac cgactggtac aatctggatg cggcggcgaa ccagctgcac ggtgcccaat gggctctctgg tgaccgcttc gtttcgcgac cttcgttcgc cagcttctgc caacacggcg gttcgact
116. tc cctcaatggac Ccgacaccggc gctgatccgc gtcgcacatg caccgccggc Cgcraccgcc ccccggccag gcgcgtctag ggtcaatgtg ggtgggcaac caccagcagc atggaagtgc tgcccgcccg ggaggaggcc cgctgcgctg gcttggcttc ggtagccggc gtcgaccegcg gcagaacctg ccggcatcgc gccagcacat agcctggcgc tgcgcgatct tgacgggcga tcgagcgctg tgcgcagatc JP 2012 70751 A 2012 4 12 ccagcagcct agetagccgt caggcgggtt ttcttgttaa cgaaagcctc agctcgaggg agggcgcaga ggggaggtty cggcgttcat agtattgcga cccgcactgg gaaatcatcc aacatcctcc ccggtgccct ggctacttct cacatgaacc cgcaacgact atcggcggcg gagcttgcca ggctatgcgc gttggcgagc agctagcagc gctctcaagg aaccccgacc tattcgcccg caggcctacg tacgactggg gtgcgcgccg gttgccagcg cgcagcggta aactttcgct gtcagccagc tatccgacct ctgatccgca caggtccagg gectcggcta ggcgacgaggc tcgtatcagg ctggcccggc accagcctgg tegegtctge tccgacgccc acgctgcagg tgatcgatgc gcagctgcag cgagctggtc ctgttcgatg 10 20 30 40 97 poooo 1 61 121 181 241 301 361 421 481 DOAGKSENAV RDFSSWSDGS GYPDKEINLP LRSQYGAGGF IIKDWSDRAK ONGGOHEWNAL SAISFHSGYS GSGGGEPGAL IALPAGONEE RYHGGDEIIL QGFEWNVVRE APNDWYNILR KSGGGEGYFW HDFNKNGRYG SDAQLROAAS AGQGFWRNDC ADPGNYPNDC DDGDRFIGGD RFDFVR
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