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1. 1 ELISA Enzyme Linked Immuno Sorbent Assay ELISA TEQ TEQ TEQ2. ELISA SPR SPR SPR 4 Eb MultiSPRinter SPR CCD SPH felg
3. ELISA Tel 0794 43 6508 R2 0 986 1000 E N a 100 oD I hr 10 dp 1 LLI 0 1 0 01 0 1 1 10 100 GC MS ng TEQ m3N 2 1000 W N Q O 100 un I 1 10 100 1000 GC MS pg TEQ g 4 100000 w 10000 N a O 1000 on 1 100 TI 10 1 0 001 0 01 0 1 1 10
4. TOYOBO SuperReading DNA Sequence Solution 125ml SRS 101 16 000 2003 4 9 TOYOBO BIOMATE BIOMATE STRATAGENE TOYOBO DNA Purification Kit PUR 101 20 000 2003 4 18 Page Set SQC 6A 60mIX 104 BI090207 14000 2003 4 18 Page Set SQC 5ALN 60mIX 104 BI090221 28 000 2003 4 18 Maxim Biotech RNA Transcription Kit T3 T7 100 SC200340 53 000 2003 5 31 Rubella Virus Primer Set Plus Positive Control 100 MBSP10585 29 000 2003 5 31
5. SPR MultiSPRinterrW MultiSPRinter CO0H 300 0 2M EDC 0 05M NHS PBS 1 COOH Ride EDC 1 3 3 NHS N PBS GO0mM 150mM NaCL pH7 4 2 MultiSPHinterw 18 IgG 100 g ml 10l 86 78
6. Upleed No z2 21 Conference Report ASP Allele Specific Primer PCR 14 12 13 85 eht HE zonE A NEI VAAR ER SNPs 1 STK ASP Allale Specific Primarl FGR i y ilden 2 M T SNP gt FA ik z 3 SNPs 3 3 d SMP BOGE AFSEAT FRHOMAA _ A r SNPs mr J 9 2 et TORA ET DAN s BR El KE E R
7. HRGC HRM5S la m 19 ELISA 0 98 8 6 0 93 0 97 ELISA TEQ 6 3 4 1 R ELISA ELISA 0 1ng TEQ mN
8. 96 X T Y 3 CC C T TT mm A vil t E _ p nu gm mm ayen ET g g sleder der ERS FRET H 2 1 07D 7 BESHE 2 P 3 TE EE 1 arve nr 22 Uplead Nove KE Me VM UV 3 470 T FN PCH 2 95C PCH 1
9. 93 50C PCR SNPs 1 FRET 4 FRET FRET 4 FRET 1 64C 70C 3 EEEEEELETEESSE M EEE d l M l Erepere 1 A HEEE 1 PC
10. EEE mRNA NTPs NTPs set 100mM Code No NTP 1 1 1 45 000 rNTP Mixture 25mM Code No NTP 21 1 18 000 PCR AFR dNTPs DNA mRNA Sephadex G 5 mRNA Buffer Mix
11. 1 1 2 3 orka 1 mRNA pre mRNA 2 3 PPPNFrvIRISTICEN R D DINDET J I UTOE NOU NEW RELEASE IST
12. TCP 4 5 TEQ 2 3 4 7 8 PeCDF 100 1 EN ELISA JIS K031 1 XAD DMSO ELISA
13. Tag pEU3 NI Tag Tag Tag pEU NI Tagg PROTEIOSW DTT His tag His Tag 5 10 G 5 Hemin Hemin Hemin Hemin Hemin Hemin
14. 2 3 6 000 2mg ml pH8 5 300ul 4 SPH MultiSPHinter w SPR IgG I g ml 8 g ml C Req Req C C CCD 96 1 SPR
15. CCD SPR SPR Voland No z2 0 05M NHS N vyrdrory succinirmidae ha EDC water soluble carboarrida IgG 1 1 B g ml IgG Heq 13 1 0 8 3 K H B
16. A549 Human Lung Carcinoma 293 Human Kidney Transformed 3 HeLa Human Cervical Carcinoma FE e e GeneErasert SB 10uxM GL2 siRNA HeLa GeneEraser W A B GeneEraser 95 100 1 GeneEraserTt B 100 Q siRNA 5 80 GeneJammere transfection reagent Stratagene HeLa pGL2 luciferase reporter 60 prasmid Dt luciferase GL2 siRNA 2 5 40 ul S 24
17. HV FOH EC fl TESTLIVER HE 18178 18 48 7 AAN TI SES Baker B NNE ANA E GREN ASS ENGER MADE RNA TESTLIVER total RNA TESTLIVERTK AGPC total RNA TESTLIVER 1 30ug total RNA ET i m 2 fri F FEF mrj hris 1 0A Aea EENI Bk ETE Handy Pestle I SE 44 Torodd g Ho DRAA AGPCIEI EG M TESTLIVER Rat
18. CAP Y Endo BIO INDUSTRY Vol 17 No 5 p 20 27 2000 K Madin T Sawasaki T Ogasawara Y Endo Proc Natl Acad Sci USA Vol 97 No 2 p 559 564 2000 T Sawasaki et al FEBS Letters Vol 514 p 102 105 2002 lt BU DU mRNA dNTPs mRNA rNTPs RHNA Polymerase http www toyobo co jp seihin xr product proteios proteios htmli
19. TESTLIVER AFE DE Tee 20 voad No z2 7 677 VE T WE Insulin dexamethasone 3 TESTLIVER ER ett MGE NN SE MLE x maras LE HI fr th bd CYPI BI H ANE CYPTB 1 1 CYPIA 1 TESTLIVER
20. MultiSPRinter SPR SPR 102 1 2 000 000 MultiSPRinter SPR 103 Y4 500 000 MultiSPRinter NHa Fv I MultiSPRinter COOH 1 4C SPRH 0 1 Y20 000 1 4C SPR 202 Y20 000 MultiSPRinter 96 0 SPR 301 8 000 14 Uplead No z2 ECHNICAL EVIEW Dioxin ELISA Kit For Gas For Ash 20004 1 1 HRGC HRMS HRGC HRMS 1 FEL 1
21. 3 UploadENoW 2 MBL M Dual Quantitative RTPCR DQRTPCR Kit Dual Quantitative RT PCR DQRT PCR MBI
22. FE BO EE GJEN EIE S S GJE S S BE kDa GJE 140kDa
23. 20 3 GeneEraser transfection reagent 9 83 90 o 10 20 30 10 20 30 10 20 30 A 40 60 B 13 i i SIRNA concentration nM GeneEraser siRNA Transfection Reagent 1 0m 4C SC204150 1 10 000 0 4ml 4C SC204152 53 000 amis Code No 8 Nore NEW RELEASE NEW New Recombinant Cytokines PEPROTECH New Cytokines Cytokine PEPROTECH PEPROTECH Recombinant Cytokine TOYOB0O 19928 PEPROTECH 80 Cytokine Code No m CodeNo human 4 1BB Receptor PT31015 Y39 000 PT31015L human apo SAA PT30013 Y39 000 human Artemin PT45017 Y39 000 PT45017L human BCMA PT31016 Y39 000 PT31016L Y13 000 human BD 1 36a a PT3005 1 Y39 000 PT30051L Y13 000 human BD 1 47a a PT30051A Y39 000 PT30051AL Y13 000 hum
24. total RNA 1 g gene specific primer RT primer 5pmol RT RUG 65C 5min 9 RT 42 50 C 50min 85C 5min 4C PCR SG B S d mme p ra dystrophin dystrophin 94C 2min 94C 2min 98C 10sec 980C 10sec 55C 30sec as cycles 650 30sec ls cycles 68C 6min 68C 7 5min DMD 9 5kb dystrophin 94C 2min 98C 10sec 60 30sec as cycles 680C 9 5min 3 GC rich long target Insulin like growth factorII receptor IGFRI 8 9kb HT PCR IGFRII 5 GC block GC 80 1 HUL HeLa total RNA 1 g oligo dT primer RT primer 50pmol OH bk 096 t NI C G A T IE l ret PCORY 4 710 44 Stepdowm PCR 94T 2min 987 10sec ME ne 74C 9min 2 987 10sec mE e 72 9min 98C 10sec F m 70C 9min 4 2e Ospe EE 68C 9min 68C 7min
25. ORRAK 4 mRNA pre mRNA SNPs x 3
26. pEU DHFR J pEU DHFR DHFR Positive Control Vector pEU3 NI in vitro Transcription mRNA pEU3 NI Q H 0 m N MEI 20 0well 0plate ia Sowell 300 ul gt AD ug Gi pEU DHFH 1 300l G0ug
27. HIV RNA FR LUTT UV RNA DNA hybridization 40 TH FE lt dn Fa bler da Emi TEN Dra Poeren ATP TTP irLdLITF sat armalng En lon 1 Uload No z2 17 GC 60 Tm 3 ATAT AT AT AT tailing 5 Biotin 1 1 Biotin TSA 0 0 1 pmol NET Gases AT VI KA MRS RA ENG target retrieval solution buffer 95 40 DEPC 37 K 0 1 1 0 ug ml in PK buffer 37 he
28. 1 seg GEIAW D 2 se 3 mg BRL BEFREOER 6 I GenBank gt 2 1 GEIAW I GEIAW A S I O X I
29. in situ hybridization ISH ISH in situ hybridization AT tailing ISH AT 40 5 Biotin 1 3 ATAT AT AT AT Drimer template ATthe DNA Polymerase dATP dTTP Biotin 16 dUTP Biotin Biotin Tyramide signal amplification TSA
30. SPR 1 3 Heq C 4 4ug ml 2ug mi Tug mi SPR signal 2000 3000 Time sec 4000 5E 09 0E 09 1 5E 09 1 0E 09 Req C M 5 0E 08 PAN 5 0 0E 00 0 3 IgG SPR 4 lgG 13 RI A C F 1 2x 103 1 5x 103 8 2 x 107 8 3x 107 Ts 1 m Ka M 1 6x 10 1 1x 108 6 4x 10 1 8x 108 4 4x 107 1 Nelson B P Frutos A G Brockman J M Corn R M Anal Chem 71 18 3928 3934 1999 2 Brockman J M Nelson B P and Corn R M Annu Rev Phys Chem 51 41 63 2000 Surface Plasmon Resonance Imaging System SPR 101 16 000 000 3 MultiSPRHinter SPR
31. TEQ TEQ ELISA 3 7 8 TeCDD TCP TCP TO Q O 80 ar S TCP 60 NN 2 3 4 7 8 PeCDF M 40 20 0 0 0001 0 001 0 01 0 1 1 2 3 4 7 8 PeCDF 0 01 0 1 1 10 100 TCP zg ml 1 TCP XB Bo x100 Uload No z2 re
32. PCR 25 amp PCR 18S RNA GAPDH L32 Phospholipase A2 Transferrin Receptor 5 z f Lane M 100bp DNA M W Marker Lane 1 5 10 housekeeping gene plus 10 10 target specific gene Lane 6 10 10 target specific gene plus 10 109 housekeeping gene o 10 106 internal Xe ROR ORRE Eg ONA Om ee Sr E PCR 1 50 Premixed Target Specific Primers Premixed Housekeeping Gene Specific Primers Positive Controls Optimized Dual PCR Buffer Enhancer Stabilizer dNTPs ddH 0 100bp DNA M W Marker Instruction Manual 6003 5 19 7 8
33. ATth DNA Polymerase 5U ul 500Ux 1 TTH 0 1 30 000 ATth DNA Polymerase 5U ul 500U x5 TTH 202 120 000 ATth DNA Polymerase AERA SETAR Thermus thermophilus HB8 DNA Polymerase 5 3 Exonuclease Usegve No z2 19 Conference Heport 3 TESTLIVER Rat 14 12 13 25 vaks GR AH SEE IV pp rdp SE ER TESTLIVER Rat
34. MEG punt SNPs 3 p HG ET a a 4 PCR P450 Usegve No z2 23 PROTEIOS Q amp A TOYOBO TEL 06 6348 3888 Q amp A PROTEIOSW lt
35. RT PCR RT PCR RNA cDNA CDNA PCR PCH DNA High Fidelity PCR RNA cDNA ReverTra Ace High Fidelity PCH KOD Plus RT PCR ReverTra Plus total HNA HT PCR 1 0kDb DNA High Fidelity RHT PCRH 10 3 ReverTra Plus High Fidelity RHT PCR goligo dT primer random primer gene specific primer 3 RT primer
36. Environ Microbiol 63 4504 4510 1997 Takagi M Nishioka M Kakihara H Kitabayashi M Inoue H Kawakami B Oka M and Imanaka T Appl 2 Hashimoto H Matsumoto T Nishioka M Yuasa T Takeuchi S Inoue T Fujiwara S Takagi M Imanaka T and Kai Y J Biochem Tokyo 125 983 986 1999 3 Mizuguchi H Nakatsuji M Fujiwara S Takagi M and Imanaka T J Biochem Tokyo 126 762 768 1999 4 Hashimoto H Nishioka M Fujiwara S Takagi M Imanaka T Inoue T Kai Y J Mol Biol 306 469 477 2001 RT PCR Kit ReverTra Plus IO 8 sr Amean Nin RIFS J N T S ReverTra Ace 50 000Ux 14 TRT 102 10 000Ux 14 20C TRT 101 15 00 60 000 First Strand RT PCR Kit ReverTra Ace a 100 FSK 101 53 000 High Fidelity PCR KOD Plus 200U x 1 200U x5 KOD 201 KOD 202 30 000 120 000 200Ux 11 KOD 203 230 000 Oligo dT 2o0 nrmole FSK 0 1 8 000 Random Primer 9mer 2 5nmoles 12 FSK 301 8 000 Usllege No z2 ECHNICAL EVIEW 81 R
37. Ulead No z2 25 EE GE EX GJEI Estrogen receptor MBP Human 82kDa FK506 binding protein Human 12kDa O O N D p52 Human 37kDa O O IN D MAP kinase Human 41kDa C terminal src kinase Human 51kDa Protein kinase C Human 77KDa O X X c Fos Human 41kDa c Jun Human 36kDa O Protein kinase Ca GST Human 104kDa O X N D Rhodanese Calf 33kDa O O O Green fluorescent protein A victorea 27kDa O Luciferase P pyralis 61kDa Dihydrofolate reductase E coli 18kDa O O B lactamase precursor E coli 31kDa O O N D ClpB E coli 96kDa O O IN D GroEL E coli 57kDa O O N D GroES E coli 10kDa O O N D KOD DNA polymerase Thermococcus 90kDa O Sarcosine oxidase Arthrobactor 43kDa O O O BamHI Bacillus 24kDa O O O Lipase Pseudomonas 30kDa O X X Alkaline phosphatase E coli 52kDa OOJ O N D Adeo CAP mRNA CAP 0
38. oligo dT primer random primer PCR A tail RNA oligo dT primer A tail 5kb random primer gene specific primer RT RT PCR primer RT primer 1 Oligo dT primer HeLa total RNA 1 g oligo dT RT primer 50pmol RT 65C 5min gt RT 42 50 C 60min 85C 5min 4 Uload No z2 PCR 94C 2min 98C 10sec 60C 30sec ls cycles 68 Xmin kb ReverTra Plus A B D Kit Xmin 1min C Kit Xmin 3 min 2 Gene specific primer
39. N 3 gene specific primer PCR primer RT primer TT ELF diik r r Pamir To oAMMiIF ET FL R GST 2 BEH IE Em ET 3 Gene specific primer RT PCR 2step PCR 3step PCH PCR S amp S Stepdown PCR HiFi RT PCR GC Dblock Hit ReverTra us GC block 4 1 ReverTra Plus 2 A HiFi RT PCR 3 B HiFi RT PCR M A Hind Ill digest 4 GC rich long target ReverTra P s W RT PCR
40. CBB AO GJE gt D Glil 23 26C 14 4 PG Mate EE pEU3 NI GJE RJE pEU3 NI EcoRV GJE 0 MCS Deletion
41. RT RNA RT primer RT 3 C D 3 ReverTra Plus CBP CREB binding protein 2 2 4 4 7 3kb 2 oligo dT primer 5kb CEF lakh g EF KER bolig pru krone her a KEN ET POOR ee ATA PE HET sti nt Ne ETE H2 oligo dT primer RT PCR dargei DHID Lid RT primer oligo dT primer gene specific primer ReverTra Plus DMD 4 1 5 9 7 7 9 5kb
42. EE GJE OHF MO pEU3 NI Positive Clone GJEN acZ X gal PCR PCH Insert Check Ready Code No PIK 15 1 PIK 25 1 EG pEU3 NI Vaod Nara 7 Ea al 0 cK Eu GJ RNase RNA mRNA pEU3 NI
43. GEIAW IST mRNA GEIAW mRNA pre mRNA GEIAW pre mRNA GEIA BLAST
44. Multiplex PCR Quantitative Comparative RT PCR QCRT PCR Competitor Competitor DQRT PCRH Competitor Target Array Target Target 1 000 DQRT PCR QCRT PCR HT PCHR x x x tr 1 PCR Target PCR
45. 2 19 22 BMTyramide ABC avidin biotin complex TSA TSA hybridization TSA DACO CSA system DAB ISH AT CSA In situ HybrAT tailing Method 1 2 Target retrieval solution 95 405 3 Cooled to RT 4 DEPC DW 5 Proteinase K 0 1 1 ug ml in PK buffer 37 1557 6 0 2 glycine Tris pH 7 6 X 3 7 DEPC DW x2 3 8 100 ethanol and air dried 9 Prehybridization with HybrAT buffer 37 30 10 Heated the hybridization solution 95 5 then onice 12 Hybridization 50 0 N 13 Washed in 0 1xSSC x2 55 15 14 Washed in 0 01xSSC x2 55 15 15 rinced in TBS 16 Attached Hybaid EasiSeal to the slides 17 Add 300 ul of ATth buffer 60 1053 18 100 ul of AT 60 10 19 TBS 0 1M pH7 6 20 0 396HsOs in methanol 30 21 TBS wash X 22 4xBlockace TBS gt 60 or 4 0 N 23 CSA G Reagents ll PK buffer 0 1M Tris HCI 0 05 M EDTA pH 8 0 HANTP mixture ATth DNA Polymer
46. TESTLIVER Rat LE dri firt CLEF E aE HTE 3 ee VE TESTUIVER OM 7E577 VE CYPTHB 1 pentoxyresorufin TESTLIVER 1 CYPIA 1
47. 000 DUE PT45026L 13 000 murine Resistin PT45028 39 000 DUE PT45028L 13 000 murine SDF 18 CXCL12 PT25020B Y39 000 2 ug PIT25020BL 13 000 murine SRANKL PT31511 Y39 000 2 PIS3ISTIL 13 000 Ug 2Ug oug rat GROS MIP 2 CXCL12 25uUg rat IL 1 PT4001 1 PT40001A Y39 000 Y39 000 PT40011L Y13 000 PT40001AL 13 000 rat IL 2 PT40002 39 000 PT40002L 13 000 rat IL 13 PT40016 39 000 PT40016L 13 000 rat Leptin PT40021 39 000 PT40021L 13 000 rat Macrophage Inflammatory Protein 1 beta CCL4 PT40009 39 000 PT40009L 13 000 rat SCF PT40022 39 000 PT40022L 13 000 UploadENoW 2 ECHNICAL EVIEW High Fidelity RF PCR ReverTra Plus HENT AMAR
48. 4 1 ISH RNA ISH RNA ISH RISH ISH AT CSA 5 HIV RNA ISH AT CSA 18 Usllege MA7 SHAT CSAR 5 1 Nakajima N Ionescu P Sato Y Hashimoto M Kuroita T Takahashi H Yoshikura H and Sata T Am J Pathol 162 381 389 2003 2 Hanaki K Nishihara T Odawara T Nakajima N Yamamoto K and Yoshikura H Biotechniques 31 734 738 2001 3 Nakajima N Sata T Hanaki K Kurata K and Yoshikura H J Virol Methods 81 169 177 1999 4 Nakajima N Hanaki K Shimizu YK Ohnishi S Gunji T Nakajima A Nozaki C Mizuno K Odawara T and Yoshikura H Biochem Biophys Res Commun 248 6 13 620 1998 5 Hanaki K Odawara T Nakajima N Shimizu YK Nozaki C Mizuno K Muramatsu T Kuchino Y and Yoshikura H Biochem Biophys Res Commun 244 210 219 1998 6 Hanaki K Odawara T Muramatsu T Kuchino Y Masuda M Yamamoto K Nozaki C MizunoK and Yoshikura H Biochem Biophys Res Commun 238 113 118 1997 7 11 75880
49. 5 DGQ http Www toyOoDbO CO jpD Seihin xr carmp mbDi50offcamp mDi50off html rTaq DNA Polymerase Code TAP 20 1 250Ux 5 000 anti Taq high Code TCP 101 100l 16 000 Nor 7 Gjsrrmeene NEW GeneEraser siRNA Transfection Reagent siRNA SiRNA e siRNA 1 e 1 2 NIH 3T3 Mouse Embryo Fibroblast e HT 29 Human Colon Adenocarcinoma 7
50. GC MS ng TEQ g 3 0 019 0 019 lt 0 010 0 00085 ng TEQ m N 96wellx 1 Tmlx 1 TCP DMSO 8mlx 1 8mlx1 10mlx 1 100 0 15mlx 1 Ligand Screening System Androgen Receptor 1 96 80C 20C 4C Ligand Screening System Estrogen Receptor 1 96 Ligand Screening System Estrogen Receptor 8C DXN 101 Y200 000 2S 8C DXN 201 Y200 000 RRKTRBEER TMB 1mlX 1 0 5mol lemlix1 10 50mlX 1 1 ARA 101 80C 20C 4C ERA 101 1 9679 1 Y88 000 Y88 000 A ELISA 1 9679 16 80C 20C 4C ERB 101 Y88 000 4C BEK 101 Y65 000 Uad No z2 ECHNICAL EVIEW ATth DNA Polymerase BUL l NZ A in situ hybridization ATtailing ISH AT HIV RNA
51. NEW RELEASE BEFREONE 1 S T mRNA GEIA
52. PT31019 Y39 000 10 g PT31019L 13 000 human TACI human STNF receptor Type I 20ug PT31007 39 000 Suge PT31007L Y13 000 g PT31017 39 000 5u PT31017L 13 000 human TGF 82 PT10035 Y39 000 lug PT10035L 13 000 human TWEAK PT31006 39 000 2ug PT31006L 13 000 murine APRIL PT31513 39 000 DUE PI31513L 13 000 murine BLC BCA 1 CXCL13 PT25024 39 000 5 PT25024L 13 000 murine Cardiotrophin 1 10ug PT25025 Y39 000 PT25025L Y13 000 Ug 2Ug murine CTACK CCL27 20ug PT25026 39 000 5 g PT25026L 13 000 DUE murine CXCL16 25Uug murine Eotaxin CCL24 0 g murine FIt3 Ligand DUE PT25028L Y13 000 Presoeel Y13 000 PT25031L 13 000 murine gAcrp30 PT45027L 13 000 murine IL 1 8 Prell IBL 13 000 murine IL 13 PT21013L 13 000 murine IL 22 murine l TAC CXCL11 20ug murine LIGHT 20ug murine MEC CCL28 PT25028 Y39 000 PT25022 Y39 000 PT25031 Y39 000 PT45027 Y39 000 PT 21111B Y39 000 PT21013 Y39 000 Pr21022 Y39 000 PT25029 Y39 000 PI31512 Y39 000 PTG150 PT25030 Y39 000 Y39 000 oug oug oug 2 Ug oug Pr21022L 13 000 PT25029L PT31512L PT31502L PT25030L 13 000 murine MIP 3Z CCL20 PT29027 39 000 DUE PI25027L 13 000 murine RELM a PT45026 39
53. an BD 3 20ug PT30052 Y39 000 5ug PT30052L Y13 000 human BMP 2 10ug PT12002 Y39 000 2ug PTI2002L Y13 000 human BMP 14 CDMP 1 10ug PT12001 Y39 000 2ug PTI2001L Y13 000 2 ug human Bone Morphogenetic Protein 7 0P 1 PT12003 Y39 000 PTI2003L Y13 000 human Bone Morphogenetic Protein 13 CDMP 2 PT12004 Y39 000 2 ug PTI12004L Y13 000 human BRAK CCL6 PT30050 Y39 000 BUg PT30050L 13 000 human CTACK CCL27 PT30054 Y39 000 BUg PT30054L Y13 000 human CXCL16 PT30055 Y39 000 BUg PT30055L 13 000 human EG VEGF PT10044 Y39 000 2 PT10044L 13 000 Ug human Fas Ligand 10ug PT31003 39 000 2LE PT31003L 13 000 human sAcrp30 Adipolean 10ug PT45021 Y39 000 2 ug PT45021L Y13 000 human GROS CXCL12 PT30039 Y39 000 PT30039L Y13 000 human HGF PT10039 Y39 000 PT10039L Y13 000 human IFN a PT30002A Y39 000 PT30002AL Y13 000 human IFN 8 PT30002B Y39 000 PT30002BL Y13 000 human IFN CHO PT30002BC Y39 000 PT30002BCL Y13 000 human IL TRA PI2ZO001RA Y39 000 human IL 18 10ug PT20001B Y39 000 eug PT20001BL Y13 000 human IL 16 121 a a 10ug PT20016A Y39 000 eug PT20016AL 13 000 human IL 19 10ug PT20019 39 000 ug PT20019L human IL 20 PT20020 Y39 000 PT20020L Y13 000 human IL 22 PT20022 Y39 000 PT20022L Y13 000 human Interleukin 13 Analog PI20013A Y39 000 PI2O0013AL Y13 000 human Interleukin17D IL 17D PT20027 Y39 000 2 ug PT20027L Y13 000 human Interleuk
54. ase DDW dNTP mixture 2 HybrAT buffer 50 ml Reagents Recipe Final concentration deionized formamide 5ml 10 20XSSC Sml 2X 100XDenhalt s 0 5ml 1x IN NaHaPO04 NasHPO4 2 5ml 50mM 200 mM EDTA 0 25ml 1mM 10 mg ml tRNA 5ml 500 ug ml DDW 34 25ml 3 Hybridization solution 0 0 1pmol ul probe 4ul ssDNA 10 mg ml 2ul HybrAT buffer 34ul 40ul 1section 4 AT tailing reaction mixture 100 xul per 1 section ATth buffer 10xconc 50ul 50ul Sul 395 ul 500 ul 5Bsections dATP 100 mM 10ul dTTP 100 mM 10ul Biotin 16 dUTP ImM 5O0ul DDW 430 ul 500 ul 5 TBST 0 05M Tris HCI pH7 8 0 3M NaCl 0 1 Tween20 LELIE 4 a ISH AT CSA HIV RNA 4 b Anti sense probe c Sense probe 4 d ISH AT CSA HIV RNA Alexa Fluor 488 T CD3 Alexa Fluor 568 4 e merge HIV RNA CDd 2
55. in17E IL 17E PT20025 39 000 2ug PT20025L 13 000 human Interleukin17F IL 1 F PT20024 Y39 000 2 PT20024L 13 000 Cg oug oug human LD78 CCL3L1 20ug PT30056 39 000 PT30056L 13 000 human MEC CCL28 0 g PT30057 Y39 000 PT30057L 13 000 human Myostatin 10ug PT12000 Y39 000 2 ug PTI2000L 13 000 UploadENoW 2 NEW RELEASE Code No Code No human NT 1 BCSF 3 PT45018 39 000 PT45018L 13 000 human Persephin PT45012 39 000 PT45012L 13 000 human PF 4 CXCL4 human slL 4 Receptor PT30016 PT10009 PT45022 PT45019 PT31002 PT20004R 39 000 39 000 39 000 39 000 39 000 39 000 10 g oug oug 10 g PT30016L 13 000 PT10009L PT45022L PT45019L PT31002L PT20004RL 13 000 human slL 6 Receptor PT20006R Y39 000 PT20006RL 13 000 human soluble Fas Receptor PT31020 39 000 PT31020L 13 000 human Sonic Hedgehog Shh PT10045 39 000 PT10045L 13 000 human sRANKL human sRANKL Receptor 100ug PT31001 PT31008 Y39 000 Y39 000 0 g PT3G1001L 13 000 PT31008L 13 000 human sTRAIL Receptor 1 DR4 50ug PT31018 Y39 000 10ug PT31018L Y13 000 human sTRAIL Receptor 2 DR5 50ug
56. rpet ae TN Je pel ye bar DEPC 12 3 HMHybridization ISH AT HybrAT buffer 3 Pre hybridization hybridization solution 50 AT tailing 2 13 15 EasiSeal ATth Duffer 60 10 10 AT tailing 3 1 0 ATthe buffer EasiSeal top liner AT tailing AT tailing 60 30 0 39 30
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