YK280 Humans-IgA (Saliva) ELISA 取 扱 説 明 書
Contents
1. ih VI lt gt Typical standard curve 2 50 2 00 1 50 S 8 1 00 a O 0 50 0 00 0 01 0 10 1 00 10 00 100 00 s IgA ug mL gt t PW A gt Added s IgA Observed Expected Recovery ug mL ug mL ug mL 0 64 8 40 108 8 104 8 103 8 160 220 8 224 8 98 2 400 404 8 464 8 87 1 lt B gt Added s IgA Observed Expected Recovery g mL ug mL ug mL 0 46 4 40 96 0 86 4 111 1 160 196 0 206 4 95 0 400 432 0 446 4 96 8 t h C gt Added s IgA Observed Expected Recovery ug mL ug mL ug mL 0 120 8 40 189 6 160 8 117 9 160 274 4 280 8 97 7 400 476 0 520 8 91 4 t EE D gt Added s IgA Observed Expected Recovery ug mL ug mL ug mL 76 0 112 0 40 157 6 152 0 103 7 160 244 8 272 0 90 0 400 441 6 512 0 86 3 lt E gt Added s IgA Observed Expected Recovery ug mL g mL ug mL 0 105 6 40 152 0 145 6 104 4 160 250 4 265 6 94 3 400 600 8 505 6 118 82. lt gt s IgA IgA IgG IgM IgE gt 96 s IgA HRP IgA a chain HRP s IgA O AU 2 3
3. 30C s Wt A AARIADS 20 ng mL 80 ERRIRE E S 5 FUT 30C we 6
4. s IgA YK280 Human s IgA Saliva ELISA v IgA s IgA Y 0 082 20 ug mL D 41 duplicate 2 v 2 5 3 y 4 v pL 80 5 BSR BOE AE WR v 1 8 6 2x 7 8 TU hE 2 8 6 TA I Fe 8 Ay MLE Bop T5 s IgA s IgA
5. PEER m 3 6 2x 7 8 VOR HEIR 96 1 f 10 pg viall 12 mL 1 12 mL 1 12 mL 1 25 mL 1 50 mL 1A 3 s IgA s IgA HRP IgA a chain 3 3 5 5 TMB 1M 1 Tween 20 IV 2 gt 1 Su
6. s IgA V Eq y 3 4 E ty 100 rpm 2 VM LE b o o E
7. 6 100 pL rpm 1 100 2 6 100 pL 30 100 pL 450 nm 620 nm 5 or 4 Parameter 1 E s IgA 80 lgA ABO v OO M Log EHH Linear M
8. 3000rpm 15 30 2 0 5 mL 10 gt Ma 20 pg mL 0 5mL 0 1 mL 0 2 mL 6 67 ug mL 2 22 0 74 0 25 0 082 pg mL 0 ug mL Hi lt UE S 3 Di gt 0 082 pg mL 20 pg mL 50 mL 950 mL 4 ex YER EK 1 2 lt 1 gt gt
9. YK280 Human s IgA Saliva ELISA m FOR RESEARCH LABORATORY USE ONLY T 418 0011 LESE H 2480 1 FAX 0544 22 2770 TEL 0544 22 2771 Website www yanaihara co jp E mail ask yanaihara co jp A 5 6 8 10 10 11 11 YK280 Human s IgA Saliva ELISA I IgA Secretory IgA s IgA s IgA IgA J 98 LRA UL 2 Secretory component SC s IgA 9
10. gt E PW A gt Sample dilution Observed Expected of Expected ug mL ug mL XI 363 5 363 5 X2 201 7 181 8 111 0 X4 107 7 90 9 118 5 X8 53 6 45 4 117 9 t HEN B gt Sample dilution Observed Expected of Expected Hg mL g mL XI 237 4 237 4 X2 123 9 118 7 104 4 X4 65 3 59 4 110 0 X8 32 5 29 7 109 4 t hE C gt Sample dilution Observed Expected of Expected ug mL ug mL XI 310 0 310 0 X2 167 3 155 0 107 9 X4 89 5 77 5 115 5 X8 42 7 38 8 110 2 lt D gt Sample dilution Observed Expected of Expected ug mL Hg mL XI 293 3 293 3 X2 145 3 146 7 99 1 X4 74 7 73 3 101 8 X8 38 3 36 7 104 4 lt E gt Sample dilution Observed Expected of Expected ug mL ug mL XI 188 2 188 2 X2 97 5 94 1 103 6 X4 47 4 47 0 100 7 X8 26 8 23 5 114 0 gt Serum IgG Human lt 0 1 Serum IgA Human 0 3 Serum IgM Human 0 0 Serum IgE Human 0 0 gt CV 2 9 5 8 CV 3 5 11 2 gt 0 082 20 pg mL gt EIA SH y 0 8539x 31 222 r 0 946 n 39
11. gt 1 80 FEAR LET gt 20 30 395 uL 5 pL 320 uL gt 2o 2 3 BTV 100 uL 0 0 082 0 25 0 74 2 22 6 67 20 ug mL x IARR 50 yL 1 DBP 5600rpm LKR 7 v RA 1 100 rpm 2
12. VI lt T gt 2 8 lt gt 6 10 lt gt 1 96 VI RA 1 Evans P Bristow M Hucklebridge F Clow A and Pang FY 1994 Stress arousal cortisol and secretory immunoglobulin A in students undergoing assessment Br J Clin Psychol 33 575 576 2 Bristow M Hucklebridge F Clow A and Evans P 1997 Modulation of secretory immunoglobulin A in saliva in relation to an acute episode of stress and arousa J Psychophysiol 11 248 255 3 Fujiwara S Yogo M 2003 Measuring salivary immunoglobulin A for psychological studies of stress and emotion Doshisha Psychological Review 50 57 81 gt T418 0011 2480 1 FAX 0544 22 2770 TEL 0544 22 2771 www yanaihara co jp ask yanaihara co jp 2015 8 25 11
13. L l mL 8 12 2 450 nm 3 0 3 4 5 6 1000 mL 7 BERE OER ER TIE gt 51 1534 901 Se 5001 02 5001 05 I lt gt 1 RETRO DARE 2
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