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1. 30 40 C 1 BSA 25mg ml 20 2ml 0 2ml Realtime Stain x 1 5mg BSA x 1 10cm x 10cm x 1 e 8cm Xx 10cm X 1 amp Vivaspin 20 10 000Da MWCO x1 2ml e Tris 50mM 100 gt 100mM 55 55 25 15 SDS PAGE Realtime stain 22. 10 15kDa 5 Note Realtime stain SDS PAGE SDS Realtime stain Tris Glycine Tris HEPES Bis Tris SDS Generon Realtime stain BSA
3. 100 10 lt 3 4 2ml Vivaspin 20 10 000Da MWCO 20mM Tris pH7 5 PBS pH 7 4 200 2 x5ml 20u 5 20C 6 5 10u 100C 3 lt 7 5 10u 1 1 2mg ml Note
4. BSA BSA PBS pH 7 4 20mM Tris pH7 5 4 Realtime stain 30 40 C Quick Coomassie p n GEN QC Stain 162 0805 113 fEL 03 3235 0673 FAX 03 3235 0669 532 0005 2 12 4 fEL 06 6396 6616 FAX 06 6396 6644 Mail technosales technosaurus coJp URL http www mstechno cojp
5. 100 C 10 Realtime stain 20u 10n 5 Realtime stain BSA Realtime stain BSA
6. 2 100 C 10 lt 3 4 Step 2 gt 10mM 2 ME DTT 3 2 0 5u 1 1 2M 2 ME DTT 50 100mM 4 100 3 lt 5 5ul 6 1 20u 1 20u 1 Realtime stain 10mM 2 ME DTT
7. 1 Realtime stain 1 1 1 Realtime stain Imidazole 75 100 50mM 25 NaCl 0 100 Glycine 100mM 10 2 ME amp 10mM 100 Urea 4M 100 T Step 1 gt 10mM 100 Step 2 ME Realtime stain BSA 5mg Realtime stain Generon
8. 1A D BSA Realtime stain 5u g BSA NuSep 4 20 Tris Glycine SDS PAGE 170V Image J A 100 C 10 B 100 C 10f C Step 1 100 C 10 D 50mM Step 1 Step 2 an Intensity mh Banm int enesity Eand lnte nsity at 1 htE5 100 C Life Technologies Xcell SureLoc
9. 10mM 5mM TCEP Step 1 50mM TCEP Step 2 10u 1 Realtime stain 100 C 2 5 Step 2 TCEP 2 ME DTT 2 ME DTT 10h Realtime stain
10. 1 5mg BSA 200u Q 25mg ml 20 C 2 1u 1 9u 1 10u 1 Realtime stain 3 1u1 1xPBS 20mM Tris HCI pH7 5 9u 1 10L Realtime stain 4 100 10 5 4u1l SDS PAGE BSA Realtime stain Q 2 5 Step 1 lt 10mM 2 ME DTT 1 5u1 Realtime stain 5u 10mM 2 ME DTT
11. Real stain User Guide version 1 3 Realtime stain SDS SDS PAGE Realtime stain 1 1 Realtime Stain FPLC Realtime stain 1
12. k Mini Cell with ncreasine tITIE and temperature Bio Rad mini PROTEAN 8 x 10cm 1C 1 during step 1 labelling 1 White screen Ts 5 Pa 0 F F F Electrodae Feducine agEEnt In SDS PAGE assembly 1D Labelling efficiency of reduced BSA during step 1 and step 2 labelline 5 rm1 reducing aEent T 4 4 NGI MC sten 1 BE stE pa CTT step 1 CTT step 2 lt 10 15kDa 2 Step 1
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