Ultrospec 3100 pro Spectrophotometer
Contents
1. 0 azt O QEL TRE ER SO 00560 LES M R Gs P ik xk iE QILAN 4 9730 5 3 OFFAL Og2. OFF ED TEL 03 5331 9336 NS e C U REAKORAIT T U ES AE AF 9 00 17 30
3. 25 05 77 OA OD SRM esso OF HADEDE gt A SR TH C R IE EXE J iin 8 Di U C
4. n on EJEJEJEJG HHHHHHHHHHHHHHHHHHH3HHHHH natura cubic HHHHHHHHHHH 0000000000000000004 0 0 0 0 0 0 0 0 0 0 0 HHHHHHHH HHHHHHHHHHHHHHHHHHHHH 00000 0000000000000000000 ODO 33 09000000 lt gt 00000 lt gt 00000 00 3 00 3 00000 0000000 0 000 UU 0 000 OOOO 00000000 Number of standard replicates Number of sample replicates V Units V Number of standards Set up If Factor Factor 0 0 Factor 0000 v Wavdength v Curve Type V V 00000000000000000030004000000000000 Concs ooo Dn Oooo 5 r3 r3 ooo 5 ooo 5 2 W ss g
5. o C Print V lt gt GL GLPCalibrationResults GLP Enabled Setup X User V User LV Operator gt HUE v Lab Name gt Instrument gt On Off On Rate
6. See ee EIFJETEJ E E E EEE EXE EJ EEE 00004 000000000 Bradford D OOOO Lowryl O Biuret D BCAH uv 2800 21 225 nm Information 000000000000000000000000000000 18 m kei Om Oo Oo O o cw Os 0000 V V Wavd ength CurveType No of standard No of standard replicates No of sample replicates Units 00000000000000000000 000000000000 000000000000 00000 Lowry Biura O0 0 BCAHHHHHHHHHHHHHHHHH gt Rezesiof HH I rien O Spire i ru O U T Faz 188088080010010800 000 30 9000000 003 003 lt I gt 000000000 ZI 000000000 19 Concs Cm 00000000000000000 ooo can en SA 6335 A lo ca ogg
7. V A A JA JA vi 00000000 00000 00000000 000000000000000000 0000000000000 000000000000000000 HHHHHHHHHHHHHHHHHH 0000 000 1HHHHHHUK enter 0 HHHHHHHHHH CHHHHHHH 00000 0000 00000 00000 29 Applications ELE EG Gn Om EE Oo Oo On Bu Do HHHHHHHHHH HHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHH 00 HHHHHHHHH v Wavdength 00000 v Factor 000000000 v Units lt gt 00000 lt gt 00000 V Auto set ref 00000 ye 0 DDD gt 00000000000000000000000000000000 00000000 v Mode 000000000000000 ParallelHHHHHHHHHHHHHHH ParallelHHHHHHHHHHHHHHHH pop No of samples 00 800 Active Reference 000000 000070 0000000000000000000 Serial Parallel 0 0 quu tut v TimeUnits 000000 v Delay 10000000 1000 V Reaction time 000000000000 00000000000000000000 060000000000000000000 0001000 V Save Method Y esll 000 gt
8. 30 Serial 0 O Load Parallel T Te SOUNDI ONHHHHHHHHHHHHHHHHHHHHHHHHH 4 00000000000000 SOUND OFFL 0000 1 function gt setup gt user V sound gt set up 000000000000000000000000000000 HHAAHHHHHHHHHHHHHHHHHHHHHHHSlopex FactorlHHHHS5HHHHHHHHHHHHHHHHHHHHHH 0000000000000000000 ostud HHHHHH HHHHHHHHHHHHHHLCDHHHHHHHHHHHHHHH OOOO Maximum Abs 00000 oooo MinimumAbs 00000 V AutoscaleY axis IfYel gt YHHHHHHHHHHH V Show Data Points IfYes gt AE V Plot graph when IfYesl gt IHHHHHHHH printing results 31 lt HHHHHHHHH PaaleHHHHHHHHHHHHHHHHHH HHHHHH Sample number Results Initial Abs Final Abs Slope Slope x Factor Result Linearity Start time 1 00000000000000 End time Auto find end If Y es gt HHHHHHHHHH points If No gt 0000000000 HHHHHHHHHH 32 Applications Standard Curve Wa EJETEJ EJ CIELEJEJ AEG ET n E El EJ EI EJ E E EH E
9. GLP GLP GLP Off Enabled 10 enter run 3 gt uz V Sound gt Print Key Function 39 n cag ole 5 gt v y ac p n OO a ZE A 0 5 8 mj 5 lt gt gt 00000000 00000000 Clock 00 0 00000007070000 Service 00000000000000000 gt stop 40
10. E EZ 1 000000 Excell 000000000000000000000000000000000000 00000000000000000000000000000000 00000000000000000000000000000000 00000000 lt J gt Center 0000000000000 o Cima 6 Absorbance Save DNA Transmission Recall RNA Concentration Clear Oligo Scan Tm Calculation Info Bradford Wavelength Scanning Lowry Multi Wavelength Biuret Enzyme Kinetics BCA Standard Curve UV Methods Substrate Concentration Info 000000000 Gn 00000000000 O 0000000 Applications etup O O I Post Run 00000000000000000000 00002040000000000000000 00000 00005 0 0 0 Auto Pin00000000000000000000000000 000000000000000000000000 200000 9000000 0000000000000000000000000 stop vio HHHHHHHHHHH Absorbance 000000000000000000000000 Set up EJEJEJEJEJ DUZ 5 n n n noo mn E CE 2 nn cesa E E G E E3
11. gt lt JA IV Abs WARNING HOT UV RADATD ni 0000000000000000000000000000000000000 00000000000000000000000000000000000000 00000000000000000 HHHHHHHHHHHHHHHH ODO OOD o OOO o Go m O r3 mn ooo z E ET ooo 8 E 53 ELE nm LI A ooo o m EJ OOD oa EJ EKE m Fi OOO 2 r3 ooo SE 8 nn n EJ AE Dn 2 m ZEE n Do 2 Z OA 20 E 8 2 8 nn n m etc E g a nm El 2 6 J O E c ooo E n 5 oO mnm i E EM E m m On O E ooo E 45 et SEI Ei E SEE m zg o EJ m aa GH 8 un El oO SEA E ag a asa Goa O 2 go o d n oog PJ es 3 Ses oo GE rnm E Ea S lt oop o8 GERE SECIE A gg oo a ATEA V Of oo EL U 3 n a nm ELE SEI 2 O jn 2 9 5 g 9 0000000000000000 Setup
12. 36 Standards HHHHHHHHH HHHHHHHH 0 0 0 0 oo Running Samples j 0 0 37 Setup User Baseline Clock Service e Display e Printer ener lg VIHHHHHHHHHHHHHH 61 0 0 Gau Use O 0 gt lt Accessory GLP 2000000 gt EJ OE r L3 L3 r3 Ooo A oO Boe So oO LEE pe E oma GOO ZE m EJ oO EJ L3 ova oo oto Poy poa ea SA O ggg FEE E Foo H El FoR n Poo m kd EJ ooo nun nnn m oca En Doo moo ooga oo ooo OCHA 38 accessory Printer Display GLP Setup run Seiko DPU 414
13. Seiko DPU414 Seiko DPU414 a Auto Print on 5 V AUTO PRINT gt AUTO PRINT 41 b 00 00 0 Cerint HHHHH DO a HE 00000000 0000000000000000000000 000 Wevescan j 002NA0000000000000000 print HHHHHHHHHHHHHHHH 000000000000000000000000 Wavelength Scanning Operatar AT Dadd Sign Date 02 07 89 15 13 41 Accessory Eight Position Cell Changer Start wavelength 300nm End wavelength 570nm Speed Fast Interval 1nm Sample 1 Q A 5 a 9 40 40 455 Wavelength 42 EL T3 BE 2 EE EI eamus EL gt ado ooo 5 B z EJ ON a ooo ex 8 a zg ooo n 3 pa ono ooo e a E Re EG m n
14. o0 om p EX Eig E ao Dn E nn 20 ox oO Ong Ta oo n oo oo 0n Sos EFG o0 oP oo 8 099 EJ m GEL pt SA OH Goo EJ Oop Ls 679 8 ES oo ooo oo ooo oo 865 FEG OB do L9S 355 go ps 5 Lu fos o 53705 os KO AO AEF E n g 005 guz OR CZE n E 2 me SOS EE ros po oo oo ooo vis 5874205 GUR ae GO 3 oO KICI EX RL E Ett CE 5 sues Ea ZEE on 20 T 407 cms O oo GEE 5 9 o gA oo O 20 n Dn 200 5 opa EJ OSE Hg E al A A SE EE GEE EX S o p ooo a9uEqIosqy ON Oo oOo L3 L3 ODO ooo OS CI EF
15. g Ooo El N Oo EJ ri p Re m S a pH x OO oO ES o 5 3 aos Ex E g 8 gos 3 mn a o N Sam oO x o 5 n r3 A S00 n gt 8 EI ENE lt EMRE SiE x s amp BEBE qx c Gia LEE TEL 25 Doado o 5 ES Erud EL OHC EL ESEE ta nc num Ej ES OAO O OOO ZO A gt gt ZOE oF rm 59 000040 190 900nm 00000000 12007 00 hm 0000000000 00000000 3000 nmmn 000000000 3nm 0000 00000 0 5 nm DO 0000000 000000 000000000000 A 3 0000 3 000A 0 010 99999 000001 0 200 T 000000 546 nni O O 0 300040 000 0 5 0000000 546nm 3 000A 0000000 05 O 000 340 nm 0 001A 1 220 nni Nall O O O 0 05 T 240 nn NaNO D 0 05 T 000000 9 O RS232C 000000 Centronics O O 210x 140x 80mm 00000 520x 370x 230 mm 0000 13 kg Og 100 240V AC 10 50 60Hz 0000 EN61010 1 EMCO EN61326 2 3 Generic emissions part 1 EM CH EN61000 4 6 Generic immunity part 1 Main harmonies EN61000 3 2 20000000 1509000
16. Absorbance Differencq 1 71000000000000000000000000 HHHHHHHHHHHHHH VIA 001000 v 002000 v Background on If yes lt N If yes AB 00000000000000 V Factorl 001040000000000000 vl Factor 2 0020000000000000 Units lt gt 00000 lt gt gt ll I1 Ii li li v Save Method If yes gt Load and Result Sample mn 000000 000000000000000000000000000 0000000000000000000000000000 3pointn amp amiotid DO BlirupnHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHH 2 001 7 002 v 003 v Factor 0000000000000 V Units lt gt 00000 lt gt HHHHH Save Method If yes gt HHHHHHHHHHHH W Load and Height Area Sample nn 000000 HHHHHHHHHHHHHHHHHHHHH 0000 0000000000000000000000000000 26 Multiwave L 0 000000000000000000000000000 2100000000000000000000000000 Set up EE n Oo oo oo Z EE c3 21 1 5 EE E a Z EJ Do EJ Lu LA u Oz N EJ a OB 4 ooo ET nm L9 oa 1 Ez DJ RD EJ Oi yasa O CIE EJ EJ EJ V 5 5 5 S o E iur lt Factors Oa o 25 E oo
17. Bem 9 ax aaa 5 8 c3 sido woo Wavelength Lu 45 BED 53 oOo ooo no qo ooo oC c3 Hu HH OOO Do il OOD ja co Et OO E lo oo Oo Do os oonan r3 an On JE EE On EE On On LZ EJ LL IZ EHE Lr ILI EE oo oo oo ooo Oooo OOO EEEEIFFT E E ET oo ET r3 ET Dn A Y A Y O E EJ pj Ej E EJ SS EEE ESTES ET ES ET EL LL 2 TE
18. E EJ o_o n oO as z7 50 Pog n DD D ca oov ngo Boo m Som Y SOO gt DT E 925 o 5 E 000000000000000000000000 Graphs HHHHHHHHHHHH HHHHHHHHHHHH 000000000000000000000000000000 If Y es Max Abs MinAbs Autoscale Y axis post run Standards 20 UV methods EJ 0000000000 000000000000 000000000000 Do Dol Do Ell N EJ Oo 8 3 ooo 0000000000000 n L3 00 00 00 00000 000000 280nmHH 0000000000 0000000000000000 0000000000000000 0000000000000000 000000000000000000000000000 O HH 0 mym 1 55x Abs wo 0 76 Abs HHHHHHHHHHHHHHHHHHHHHHHHHHH 260nmHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHXH YOD 0000000000000000000000X00000000000 HHHHHHHHHHHHH260nmHHHHHHHHHHHHHHHHH 000000000000000000000 0000000000000000 000 Warburg and Christian Biochemisches Zeitung 310 384 1941 CIE D Factor 1 Factor 2 V V V V Lo
19. oo q E o EE nz pm Ei Or nu pz ETF o 2 8 ZE pm a Or OTO ES OI o OR OO EE E EJ E e EE EJ o0 O n El SSeS o a oS oo oo Oo ado o ooo oo oo L Soe El ORDO Ej Se agH Ez EJ EJ 53 r3 r3 oO SES Ed n m E oO oo o 53 GE oO ENE 53 EE GTE DI DAE s EE naa O E OO E Eit 2059 ax El 2 n am EH n am 52282 n n OO ZE CI EE EE oo CE ooo CEJ SSeS oo a ooo L1 ooo 00000000 00000000 E ET TO YOUR EYES HHHHHHHHHHHHHHH HHHHHHHHHHHHHHH 0000000000 AN WARNING U V RADIATION IS HARMFUL
20. gt 5 gt O lo S 2 8 5 D 5 2 p 5 6 5 5 2 S 6 5 old E 5 2 E o Z lt 8 8 E 7 e oj jo lt in status bar C 1000000 1 0 1 1 0 0000000 000 HHHHHHHHHHHHH 80 2109 70 040000000 OmmHHHHHHHH 0000 HHHHHHHHHH 80 2106 01 100000 0 1 5 000000000000000 80 2108 01 1 ELI 0000000000000000000 80 2106 04 46 11 1 I1 i Li 0000000000000 0000000000 10mm 0 80 2106 05 00000000000000000 HHHHHHHHHHHHHHHH 0000000000000000000 000000000000000 80 2106 06 80 2106 09 70 ull 0 80 2103 69 HH 1000 21000000000000000000 47 HHHHHHHHHHHHHHHH HHHHHHHH 2 00000000 80 2106 12 48 HHHHHHHHHHHHHHHHHH 80
21. cd N z mu pbi CIECIE EJ 3 ou oooo Op O ad o E EL EJ a E E EJ Ej E O 5 ET EJ n A lt Y gt gt b 27 Equation 1 and Equation 2 v Description v Equation V Units lt gt V lt gt Enable Equation IfY es Save Method IfY es 000000 000 HHHHHHHHHHH HHHHHHHHHHH gt Load and nn 000 Cnn ooo 0000000000000000 Equation 1 Description Equation 2 Description lt Equation 20 00000 enter HHHHHHHHHHHHHHH HHHHHHHHHHHHH 28 Multwavel LU uti OOO Cobalt gif 0 0 1226x A 03x A 0 x 100 511 Nickel 0 0 40x A 27 41x A f1 x 100 000000 0000 No of 5 2 511 A 720 Integration Time 1s 000000 0000 C 100 K1 12 26 K2 0 30 K3 040 K4 27 41 Equation 1 and Equation 2 Description Equation Units Enable Equation Save Method 511 Cobalt
22. lt lt gt y o GOO E A 02 on ooo 5 o ZE poo g OO rm aan Sag l jes E coos ET gt gt lt oo E 34 Applications StanderdCurvelHHHHHHHHHHHHHHHHHHHHHHHHHHHH 000 LEE EE ji l1 j i i li 4 01 1 Set up v Wavdength v Curve Type HHHHHHHHHH 00000 009 003 003 lt gt lt gt 00000 00000 v No of Standards v No of Standard Replicates V No of Sample Replicates V Units 4 HHHHHHHHHHHHH1HHHHHHHHH 0 0000 0 0 9 0009 0 D 00 000000000000000000034000000000000 35 v Timeunits 00000 v Dday 000000 v Reaction time 000000000000 20000000000000009 V 0000 Save Method gt Load and 000000000000000000000000 U HHHHHHHHHHHHHHHHHHmodmdHHHHHHCconcs HH 00000000000000000000000000 m 0000000000000080000000000000000000 0000000000000000000000000000000 HHHHHHHHHHHHHHLCDHHHHHHHHHHHHHH 0000 V MaximumAbs 00000 v Minimum A bs 00000 SR wr IfYes gt 000000000000
23. oo BE a End o ET GEE Z Hu Z o E EI On E EI E REG ZE a n o Goo Y OO ogo ono gt 030 OO EE r OO ooo Do BAO oS ET OOO ooo g g poc coo OOO Lr OOO ODO j s EEE HA ada g aaa kiusu E AAO BA c 2 ooo CE p g 8 ooo E A 3 ul E 1 Graph HHHHHHHHHHHHHHLCDHHHHHHHHHHHHHHHHHH r3 m r3 E t ELE n DZ oo ODO GR oF oe pt CELI n HD Em E VE na o 53 EJ 2 0 22 85 6 BBE EJ p A 88 85 g ss tj A E Pu o2 r3 p 200 E AAA oH E EEE EL ano ada Do GF m ooo O o mum n a 8 98 O mnm BOB
24. E AE EES 522000 E Ei peg 13 AAS EE ZE EG Ez Zi 050 5000 EZE ZER ETE EJ r3 EJ EJ EET endo FEE ET 3 ZOO O OOO OOO CI ET EJ oom OO EE oH EE MEJ ZE ME cg ooo ER GEE ZOE pon nnn SS5a2 0088 AS 02000 OZ PODA n n O OO ODO ELETETEEI
25. ParametelHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHH Concentration UV methods 000 000 KEE Cod m 458 0 1234 Sample 1 TO Tisa 86 4 raps 5 8 REH BT PRA TE TTT ET ndis vt ra ra li ae Wavescan 433 0nm 0 Bara 09 52 cena 360 UV Vis 400 420 440 460 480 500 520 540 560 550 Press lt run gt on Sample 25 0 20 0 15 0 10 0 05 0 00 0 Absorbance O 0 0 O Transmission J Nucleid 0 0 0 DNAD RNAQ Oligo Protein HHHHHHHHHHHHHHHHH diu 000 00 0000000000000000020000000000000000 Basi Og u U 3 U OOOO E
26. ontav eno 2 AR IF EAS 76 X IC ik Sj st Ej gt IE REE 7 3 DU ty 0 ZAIN NES E 60 2383 At
27. 2105 49 000000000000 80 2112 15 80 2112 13 00000000 80 2106 18 80 2106 19 80 2107 14 HHHHHHHHHHHHH HHHHHHHHH D D D 10mmBot 00 80 2108 10 49 SWIFTIIHHHHHHHHHHHHHH SWIFTIIHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 0000 80210826 SWIFTII LAB 0000 000000000000000000000000000 80210831 SWIFTII METHOD0 000000 0000000000000000000000000 000000000000000 00000000000000000000000000000 80 2107 88 SWIFT II SCAN Wavelength Scanning 000000 80 2107 8 SWIFT II KIN Reaction Kinetics 000000000 80 2107 90 SWIFT II TIME TimeDrive 0000000 80 2107 91 SWIFT II QUANT Quantification 80 2107 92 SWIFT II MULTI Multi Wavelength 0000 80 2107 93 SWIFT II FRAC Fraction Analysis PCHHHHHHHHHHH HHHHHHHHHHHHHIBMHHHHHH486HHHHHHHHHH HHHHHHHHHHHHH Microsoft Windows 95 98 0 0 i HHHHHHHHHHHPCHH8Mb RAMHHH 500 MbHHHHHHHH 535HHHHHHHHHHHHHHHHHHHHHHHHHHHHH COMMS 000049 TM0000000000000000000 Tm HHHHHHHHHHHHHHHHHHHHHHHHHHH1HHHHHHH VeGAHHHHHHHHHHHHHHHHHHHHHH Microsoft Windows95 0000000000000000000000000 0000000000000000000000000 Windowd p00000000000000000 51 UU GLPIGMPHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHH ISO 90010 D 1 00000000000000000
28. a EX ooo 5 ug B EIC da n E ET Sa e SEL RE JR 5 82 EA g2 ga once 7 On EE JE bw 9 co cce E OS FE ee E 8 EJ SE OGG H EG 8go ga y ano Sa do CE oO y 2o go go oF Og nung Po uH m ya Ej pocoo oo GFT aro GE aa pogo 420060 oo ooon oo OOOO BOOED oo ay nS i 0000 aa oo m Dog DO EJ o 8 o o oot oo 8 o o n E EJ EJ oop 38 n do r3 ey RJ A n z 8 ao na zz o o oF o ooo ug ga ZO o aah se EJ oo BR HE SEL
29. nnn ZEE Ooo EEE Foo Ooo See S55 52 EJ EJ oss Eso pau GO pau GO GEE ZE m ELE ETE ESTE Set up g 5 c o S n o Sm SET B a oo o DD a DD m 030 o ZOE m n D g EE a o os n 8 20 gg ola ZE GE ea Ro MR ex S EE um pu 5 B ol EE OO OO m Dn A A A A 5 glo Li E 8 y E gt gt O Ke vw oF HE 5 Ej E Ej EI E o EJ o EJ x a 5 8 E Pz Ek E B v s Ww A A gt gt gt gt gt gt 0 5nm 1 0nm 1800 750 250 00 Fast 375 125 Medium Slow 00 23 Graph 00000000000000000000000000000000 00000 00000 0000 0000 Y0000000000000000000 00 gt 000000 000000 AIHHHHHHHHHHHHHIVMIHHHHHHHHH HHH
30. 000 n 0000000000000000000 maintenance Certification 00000000000000000000000000000 ETE El EJ EE El El El OO EJ L3 H 00000000 A CN m St 52 00000000000 00000000000000000000000000 0000000000000000 0000000000000 000000000000000000000000000 00000000000000 n n n n oo a EEE O El oo a 1 oo a m EE a EE m E EHETE Em EIET EI Ez ET EJ a Daio ao El El El zl O Ez Ooo Ed E r1 m oon EJ ET EEEE EJ EIGE EJ EJ Ooo EI oom EJ OOO EJ ET EJ r EJ 1 EJ El EEE EJ r3 EE ESI EJ CEE EJ EJ Ea EA EI EJ E
31. 00000000000 v Save Method fys gt 00000000000000 Yes Load and A Ratio Concentration Sample 00000000 Crn HHHHHH 0000000000000000000001000000 0000000000000000000000000000 000000400000000 lt 000000000000 16 Tm Calculation HHHHHHHHH 0 0 000000000 pmol 4 0000 0000 00000000 E 2 5 L ET n EJ n O El og oo n go o aa z 9 E Ei z EI AE rhe ZZ g Fo ZE Ho n oo ET n o m Hu Y s as r3 EA x oo z An C zB EJ Z o Cano EJ Ooo ZO RO nu yx Se AA EL 3 mr lt 66 RE GE ELE E o HE a a 85 N A 0 lt lt 8 5 g E m O m gt gt gt gt 0000000000000000000000000 17 EJEJEJEJ Oooo El DO E 1 ES om EJ om
32. 00000000000000000000000000000000 00000000000000 0002000000000 20M 000000000000000000000 000000000000000000000000000000000 20000 00000000000000000000000000 0000 2HHHHHH260nrHHHHHHHHHHHHHH 280mm 00000 00000000000000000000000000 000 00000 x00000 OO 00A 20A 24x00000 0000 ut 00 00000 ugm A yog I A x 280 ds DNA 50 18 RNA 40 2 0 HHHHHHHHH 33 HHHHHHHH 000 lj li li l l l ji i i i li li li li 000000 0000000000 270000 00000000 350nr1 0000000000000 O U O 6 Scan O 00000 Multi Waveengh QO D D AbsorbanceRaio ll IJJ HUU D DNAD RNAI Oligo 000000000 10mmHHHHHHHHHHHHHHH 00000000000000000 49M000000000000000000 00000000 50 4033000000 5mmHHHHHHHHHHHHHHH 00000000000000000000000000000 ul 000 Sm 0000000 DNAHHHHHHHHHHHHHHHH 100000 000000 000 10mmHHHHHHHH10HHHH DNAHHHHHHHHHHHHHHH 200HHHHHHHHH HHHHHHHHHHHH5MHHHHHHHHHHHHHHHHHHHHH 00000000000000000000000000000000000 0000000000 00000000000000000000000000000 HHHHHHHHHHHHHHHHHH 320nmmHHHHHHHHHH 0 E 3 OC L3 r3 00 UU 00 00 Molecular Cloning Maniatis et al m 260m1 mt 1000000000000 OnrHHHHHHHHHH 0000000000000000000000 14 ONA and RNA 0000000000000000000000 V V V V Background on
33. 11110000000000000000000 00000000000000000000000000000000 0000000000000000000000000000 OOO OOD OOD OOo 60 2007 GE GE RW FE ns gt la T169 0073 B E 3 25 1 ko ISO 9001 2000 TEL 03 5331 9336 FAX 03 5331 9370 e mail Tech JPOge com Home Page http www gehealthcare co jp lifesciences 21 yam yeas GE Healthcare r gt ZE LOCIE
34. 18 T PASS Xe lamp 99 of original energy Current instrument state Accesory Eight Position Cell Changer Xe lamp installed 21 11 00 14 14 use 0 hours baseline in use 21 11 00 13 12 baseline stored 21 11 00 13 12 GLP EnabledlHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 00000000 56 Kinetcs7 0000000000 ALT LDH HH340nrHHHHHHHHHHHHHHHHHHHHHHHHH 0 0 2 00000000000000 lt 000000000000 HHHHHHNADHH 000 NAD 000 000 Time HHHHHHHHHHHHHHHH HHHHHHH HHHHHHHHH a lt HHHHA BHHHHHHHHHHHHHHHHHHHH HHHHB CHHHHH OOOO DO U 57 00Bers 00000000000 dC dt dA dt x 1 EL dc dt OO li O mol 1 LED D D D U lar EHHHHHHHHHHHHHHHHHHHH NADHO EL 6300l mol cm 00000000000000000000000000000000 dC dt x VYV5 VHHHHHHHHH VHHHHHHH 0000002400000000000 DUHHHIUHH 25 Q 0010000000 10 0000000 HHHHHHHHHHHHH HHHHHHHHHHHHH 2 Katal kal 7010000100010000000000000000000 Ika 6x 101U 10 1 67 SIHHHHHHHHHHHHHHHHHHHHHHHHHH HHmol IHHHHHHHHHHHHHHHHHHHHHHHHH atal 000000 HHHHHHHHIUAHHHkeMIHHHHHH 58 1 51 Factori Vt 10 E L Vs OOO ooo GE ooo a E ooo El Ooo nos ca Do ES OR m
35. 3 Cc E 2 iim Dn n naqan t HOO EJ Oo krt Oo 6 EE c oo S a 9 m S 2 2 a 5 5 9 o s s E E EJ o c 3 A O EYETETET wy NE gt gt 2 T Iransmission Set up Cell number Wavelength V Transmission Sample Wavelength Cs Concentration e 8 EE am Dn E BID 2 E Eee Bass oon E gt Dos Dos RI ra
36. Dilution factor Integration Time Save Method If yes lt 00000000000000000000 If yes gt 00000000000000 Load and nn 00000000 mn 000000 0000 lt lt lt lt Ratio Concentration Sample 000000000000000000000000 oligo 0000000000000000000000 Background on If yes gt HHHHHHHHHHHHHHHHH Dilution factor 00000000000000000000 Factor 00000 33ugm Integration Time Save Method Ifyes gt HHHHHHHHHHHHHH Load and run no 000000 Ratio Concentration Sample un 000000 00000000000000000000 000000 HHHHHHHHHHHHHHHHHHHHHHHHHHHH 010000000024401000000000000000000000000 0000000000000001 integration tim 000000000 15 Scan 0000000000000000000000 2000 350nmHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHH v Enda 0000000 350 eoo nri 00000 000000 VA 000000000000000000 V A 000000000000 2400 Background on If yes gt Background 0 0000000 If yes AB Background 0 O V Factor 000
37. GE Healthcare TRDE Ultrospec 3100 pro GE imagination qt work 71 1831 34 CONTENTS Page Section 2 0200000 3 3 HHHHHHHHHHHHHHHH 4 20000 9 HHHHHHHHHHHHHHH 6 U0 6 OOOO 7 020000 8 UUU U U U 9 Basic Abs T 12 Methods 13 Nucleic 18 Protein 23 Wavescan 25 Multiwave 30 Kinetics 33 Standard Curve 35 Substrate 38 HHHHHHHHHH 4 02000000 43 HHHHHHHHHHHHHHHHHH 45 20000 46 1111111011 U 46 HHHHHHHHHHHHHHH 4 HHHHHHHHHHHHHHHH 49 HHHHHHHHHH 50 0000 5I SWIFT100000000000000 52 52 HHHHHHHHHHHH 52 0000000 53 HHHHHHHHHHHHHHHHH 54 54 Good Laboratory Practice 57 Kinetics 0000000000 60 D D ma Ez 5 Oo oo a nm 5 ITE A E Bru OA To Or OA 5 EE A Bru A o OAO OA m O Or a E Er El En OR O 5 F O eu O 7 En m mn EI oO EJ n EJ EJ nn EJ EJ ao El nn g ay E m ay
38. HHHGLPHHHHHHHHHHHHHHHHHHH HU 000000000000000000000000 00000000000000000000000000000000000000 0000000000000000000000 GLP CALIBRATION FAIL 0000000000000000 ee0000000000000000000 0000000000000000000000000000000000000 HHHHHHHHHHHHHHHHH GLPHHHHHHHHHHHHHHHHHH HH 0 0000000000000000 000000000000000000000000000 0000000000000000000000000000000000 EL 0000000000000000000000000000000000 0 0000000000000000000000000000000 54 GLP CALIBRATION FAIL 100000000000000000000000 erter 00000000000000000000000000000000 HHHHHHHHHHHHHHHHHHHHHGLPHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH m HHHHHHHHHHH GLPEnapleiHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHH 1000000000 LCDHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHH HHHHHHHHHHH 55 HHHHHHHHHHHH 0 GLP Enabled Ultrospec 3100 pro UV Vis Spectrophotometer Lab Names wora k Yok A Instrument Ultrospec 3100pro Serial number 81410 Software 4194 V1 1 Slave 4194 V1 2 Last Serviced 20 11 00 17 05 Instrument state at calibration GLP Calibrated 05 02 01 22 45 Calibration Full UV Visible Bandwidth 2 0 3 0nm 2 5nm PASS Wavelength 881 9nm 881 9nm PASS Absorbance at 220nm 1 382 1 396A 1 388A PASS 340nm 1 229 1 253A 1 241A PASS 500nm 1 129 1 141A 1 135A PASS Stray light at 220nm 0 050 T 0 0
39. HHHHH HHHHHHHHHHHH 000000000000000000000 000000 0000000 4 lt gt 000000000000000000000000 0 Overlay L3 C3 C3 E3 EJ S 000 Off 1 2nd 4th Derivative Enhance Transmission HHHHHHHHHHHHHHHHHHHH V Type v Scale Offset U Peak Table 24 Applications AbsorbeanceRaiolHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 00000 0200000 va 001000 v A 102000 V Background on If yes gt 0000000000000000 N If yes AB 00000000000000 V Factor 000000000000000 v Units lt gt nn nun lt gt 0000 SaveMethod If yes gt Load and Ratio Concentration Sample mn 00000000 Cm 000000 0000000 0000000 25
40. I El Eli HO El r3 ES EXE E Ea E r3 El EEE El EI El EJ ETTI ETE ES EI pa E EE E EEE En EJ ELE EJ EEE E ET EHS ET ODO ODO EFE ETE ODO ODO ODO 1 1 o 1 53 UU Good Laboratory Practice Good Laboratory PractceHHHHHHHHHHHHHHHHHHHHHHHHH 0000000000000000000000000000000000000 0000000000000000000000000000000000000 00000000000000000000000000000000000000 0000000000000000000000000000000000000 000000 GLPPRINT OUT I 07N00000000000000000000 000000000000000000000000000000000GLPOOO 0000000000000000000000000000000000 GLP PRINT OUT 07N00000000000000000000000000000 0000000 GLPHHHHHHH HHH GLPHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 000000000000000 0000000000000000000000000000000 0 881 9 OOOO 8819nmHHHHHHHHHHHHHHHH 00000000000000000000000000000 000000 HHHHHHHHHHHH
41. Q o0 me j Es ooo go o oo DOR Do mum O mn ZOO of ooo 2 ooo Z ovo om o oad o ue so a ZZA oo mom do o o um EE E SER ATE ooo non EJ Z ooo oo SKIE OOO oo so Foe OOO oo n ooo SE SE 5 ess a HD ooo oo sto Soo oo g o EE oo eo Ogopa OSS oo BE ORPoo 8 goo ELE Esooogoo8oo PBspo 2 8 ERD 353000500200 w O EZ cm 303 3 2 2 a 2 OOD g oo aaa DAD 000800 uu Ec oO Of maaa gogo aaa e 200050500 ET EJ 43 x n o ug Er o o 2 D pb 80 om E imo EE an oo So ze CEJ Z as Opo no w oo EJ E DZ oo T Off O v8L o EZ o o EM 56 ES GE EJ DD EE
42. Z E Standard 1000000002 HUD mn 10000300 Wavelength Concentration Sample 00000000 0000 00000000 UNE 00000000 000000 11 Recall 0000000000000000 Clear 11 00 al O center good 0 N Save 00000005000000000000000000 1 0000000 0 00000000000000000 Pt HHHHHHHHH 12 co00000 6800000000000 58 205 EJ o oo E E 2 BL ao Dota EJ EJ ZZA r3 ee EB o n EI RD DD S O 8 comu Sc CESSA Se 22950000 Bess lt z nao c3 562 EJ 3222 8 Eg m OA lt O 3 ES 13 A 20 Aim A 260 A azo 00000000000000000000000000000000000000000 0000 A A 280 A 260 HHHHHHHHHHHHHH A A200000 DNADODO 187000 RNA 20000000000000000000000000000 00000
43. a nun aop 5 5 Fao Boo pogon Bo goo vimm orn Oo 529200 tO Sox O geo Onn a 6 4 s Roo ol HHHHHHHHHHHHHHHH 00000 gt Factor Standard 00000 Factor Cell number Mode Wavelength V V Factor Concentration 000 x00000 000000 ri EJ EJ EJ E E AE EJ Ex 7 dm o EL EX e 8 n E a Ba oa EJ EJ EJ aoe EE Sm c oo Ono o oo LTE am Mos 8 GO Vian 2 o o lt 9 gt 5 w IS S 5 y Ag o RE 5 gt EJ gt gt gt 10 Standard Concentration Standard 0000000x00000000 0000000 HHHHHHHHH HHHHHHHHHHHHHHH v Standard 0 0010 99099 v Units lt gt 000000000000 lt gt 000000000000000001 S
44. ad and Save Method yes 0000 0000000 0000 1x Abs ABS zo D I I D 2x Abs Aks 3200000000000000000000000000000000000 00000000000000000 200000000000000000000 2 000HHHHHHHHHH OO BSA bovineserumabumin 00000000000 11414500000 HH 08mgmHHHHHHHHHHH Background on yes 00000000000000000 00000 10000000000 00000 155mgmi 00000 20000000000 00000 1 HHHHHHHHHHHHHH Cn 0000000 Cra 00000 21 Empirical 280 205 0000000000000000000000 00000000nm9m00000000000000000 00000000 mom 27 120X AJA os 00000 Scopes R K 1974 Measurement of protein by spectrometry at 205 nm Anal Biochem 59 277 282 V Save Method If yes gt Load and fm 0000000000000000 00000000000000000 Ratio Concentration D 00000000 Empirical 215 255 nm HHHHHHHHHHHHHHHHHHHHHH HHHHHHHHugmHHHHHHHHHHHHHHHHH 00000000 mam 14x A gt of protein J Lab Clin Med 48 311 314 V Save Method If yes gt Load and fm 0000000000000000 fm 00000000000000000 00000000 Result 00000 Waddd W J 1956 A Simple UV Spectrophotometric method for the determination enter HHHHHrmgmHHHHHHHHHH 22 Applications ce Wavescan ooo ado aaa AE EEE
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