DNA-S取説 - 株式会社リーゾ
Contents
1. DNA DNA 3 7O SIGMA Cat No P2069 15 O0OO rom 20 25C 10 4 DNA S2. DNA F013F01 S 70 TE 10 mM Tris HCL 1 mM EDTA pH 8O 7 3 DNA F013F01 S DNA 1 30 60 mg 2 1 5 ml 2 OMA 40O 1 1 3 15 OOO rpm 20 25C 10 4 200 gl 1 5 ml 20O wl 5 15 OOO rpm 10 6 70 8OO 1
3. DNA DNA CPO3 PCR M 1 2 3 4 5 6 7 8 124 bp M 981011 12 13 2 Agarose M Marker 1OO base pair ladder 1 1 8 2 1 9 g 3 10 og 4 11 5 Cg 6 12 og 7 13 1 PCR Template DNA 1 6 a 0 10xBuffer 3 dNTP mixture 2 0mM each 3 primer 4 pmol each 1 3 Taq 5units 1 25 HzO Total 30 ul DNA 10 100 Stratagene Paq5OOO PCR 95C 2 min 94C 30 sec 6OC 30 sec 72C 30 sec 72C 7 min 40 cycles DNA F 01F01 S DNA 2 Agarose M Marker 1OO base
4. PCR 3 SIGMA Cat No P2069 15 OOO rom 20 25C 10 4 2 65C 30 3 DNA DNA F013F01 S DNA
5. DNA S OOO7 V CDNA Ver 1 01 RIZO Inc DNA 8 9 DNA S DNA DNA DNA PCR DNA 85mL X 2 420 4C
6. 7 15 000 rpm 4 10 8 9 5O 1OO1 TE POR DNA F 01F01 S DNA PCR TE PCR
7. pair ladder 1 1 Nucleotide pyrophosphatase precursor 9GOO bp 2 5 Aminophospholipid fipase 10 825 bp 3 12 UDP glucose 6G dehydrogenase 8O9 bp PCR Template DNA 1 ub 10XBuffer 3 dNTP mixture 2 0mM each 3 primer 4 pmol each 1 3 Taq 5units ul O HzO 25 Total 30 ul DNA 10 Stratagene Paq5OOO PCR 95C 2 min 94C 30 sec 55C 30 sec 35 cycles 72C 60 sec 72C 7 min 2 9 2 B201 Tel 029 852 9351 Fax 020 4623 561 1 E mail info rizo cojp http www rizo co jp Copyright e2009 2010 RIZO Inc All Right Reserved
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