B1-RD1
Contents
1. No Wash No Wash Q PREP TQ Prep Q PREP TQ Prep T B B12. 4 FS 90 SS 1 2 3 EDTA 6
3. 10 11 12 13 90
4. MslgG2a RD1 B1 RD1 FA 6604119 50 0 5mL EDTA MRE 1 100 uU L 1 1 2mL 3 10 x 10 A mm 10 x 10 mm 3 x 10 mm Q PREP PBS 20 25 6 a
5. TQ Prep Multi Q Prep B B IgG B1 RD1 BB CD20
6. Mo2 RD1 KC56 FITC CD14 CD45 Fc MSlgG2a RD1 1 29 29 T T Harvard Medical S
7. 1 10L TQ Prep Multi Q Prep 7546999 300 TQ Prep Multi Q Prep FA 3 A B C AEFI 2 1 6603152 300 1mL PBS 24mL 9 259 9 PBS PBS 6603369 1 500mL pH 7 2 0 2 2 VU 3
8. B1 RD1 B CD20 1 Q PREP ELE TAXAMETER CD20 Flow Count LH700 mm nm x x 10 CYTO TROL 6604248 Fc
9. 135 0063 5 7 TOC Baconn COULTER 6603858_0911 4 4
10. 9 CV 10 1 0 1 2 3 4 5 6 1 2 8
11. 6603858 9009 11 2007 2 20300AMY00167000 877449 B B1 RD1 _ 2 4 wo RD1 Phycoerythrin PE 1 B1 RD1 0 2 1 2ug B1 CD20 30 35kD CD20 tld AAAI UO NB BRE AA BRED ODES TE B T MRD BER FABRICA
12. 2 8 C 24 4 5 6 7 2 1 2 100L 10L MSlgG2a RD1 10L 45 PBS 2 3mL 400 450 x g 5 LET PBS 25 1mL MATKHEL 30 2 250 kL MA BHLET 9 PBS 2mL 10 400 450 x g 5 11
13. CD20 19 95 n 102 BI 4 209 Sao ee ee ne 3 4 B1 CD20 B1 B B1 Pokeweed mitogen PWM CD20 ET 3
14. 12 9 11 13 PBS 500uL 14 EPICS 1 2 Q PREP EPICS FS Q PREP 0 5mL 3 AE NER BBR FR AER 3 RD1 PE Log 1 3 4 5 6 7 8 CD20
15. 2 20 25 3 B1 RD1 6603858 50 0 5mL 1 Stashenko P Nadler LM Hardy R and Schlossman SF 1980 Characterization of a human B lymphocyte specific antigen J Immol 125 1678 1680 2 Reinherz EL Haynes BF Nadler LM and Bernstein ID eds 1986 Leukocyte Typing II New York Springer Verlag Vol 2 p 8 20 3 Morimoto C Reinherz EL Nadler LM Distaso JA Steinberg AD and Shlossman SF 1982 Comparison of T and B cell markers in patients with Sjogren s syndrome and systemic lupus erythematosus Clinical Immunol Immunopath 22 270 Hutt 7135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 BUS ARTIC
16. gt 10 x10 mm BRE 10 20 x 10 2 20 30 x 10 3 30 40 x 10 5 40 60 x 10 6 60 100 x 10 10 100 200 x 10 20 B 3 x 10 mm 1 25C 500 x g 5 2 3 4 LH700 5 10 x 10 mm 1 100L 1 Q PREP TQ Prep Multi Q Prep Coulter Immunology 3 A B
17. C TQ Prep Multi Q PREP 1 12mm x 75mm 2 100 L 2 4 3 10L MSlgG1 RD1 MSlgG2a FITC 10L in 10 12 TQ Prep Multi Q Prep 25 35 EPICS 2 2
18. CD20 B Fc B1 H299 CD20 B BALB c P3 NS1 AG4 Ig lgG2a H kL B1 RD1 RD1 Phycoerythrin PE RD1 0 5 1 5 486 580nm 568 590nm 1 0 5mL BSA 0 2 0 01M NaCl 0 15M NaNs 0 1 1 4 B B B CD20 488nm
19. chool Dr S F Schlossman Coulter Immunology T B null 3 Pan T Pan B T B pan B CD20 B1 B
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