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1. Carl Zeiss LSM700 ZEN 1 2 ii 3 4 Saha wees 1 1 1 Windows2. Carl Zertss ZEN lite Windows OS http www zeiss co jp microscopy 2 Export 2 Ism czi a 12 Carl Zeiss LSM700 ZEN 1 Ism czi Save Image Fig 28 New Image File Save Save as Fig 28 peicher
3. Bit Depth Bit Depth 16bit 65 536 12bit 4 096 8bit 256 45 2 Carl Zeiss LSM700 ZEN Averaging Averaging Fig 25 a Acquisition Mode Plan Apochromat 20x 0 8 M27 Frame 512 Fig 25 Acquisition Mode Line 4
4. Workspace Configuration 2 Carl Zeiss LSM700 ZEN 2 Locate Oculars online a Locate LY LED YYLES COY ILE CIEE AE O BH
5. 1 LSM 34 Carl Zeiss LSM700 ZEN 9 3 1 Ph1 lt 3 1 2 9 4 ON 1 3 2 2
6. 27 eee Carl Zeiss LSM700 ZEN 9 1 1 Locate Online Offline Tiss es cel Brit Of 2 TFT HAL On Off ON 3 H 4 LD N WwW B WN Smart Setup Experiment Manager Light Path
7. 2 3 n 85 15
8. Fig 50 Player 2 Carl Zeiss LSM700 ZEN 8 4 Graphics Sa Auto Color Fig 51 Graphics Angle More Coordinate Cutregion Ei 6
9. ON OFF Bes Overy Closed Fig 8 Axio Observer Z1 nguraiHiGn ee Lt oo DAPI ATC Rhodamine OFF DIC Fig 9 Carl Zeiss LSM700 ZEN 3 Oculars offline Acquisition
10. Lasers 488nm 1 5 a ere Carl Zeiss LSM700 ZEN Range Indicator Master Gain Digital Offset Range Indicator Dimensions Ch Ei Fry VANSL Range Indicator Dimensions Chl TNT ea Single Channel Range Indicator wf Merged Reuse Fiq 22 Dimensions Blue BBR A 0Uyot SeHWAS UALR Magen Cyan ex Color LUT None Range Indicator
11. PE A nN aor Channel Range indicator Quick Color Setup Reuse Crop PaA La RRO HATES ED Fig 48 Dimensions 2ch Zoom Merge Single oe sauce cle Channel s ON OFF Crop PHYA Reuse 8 2 Display Desplay Auto Min Max Bestfit nan oo 8 3 Player Z
12. 0 Gain Master Digital Offset Fig 23 Range Indicator z 1Airy Fig 17 Gain Master 800 1000 Fig 21 Channel Fig 21 a TAs Carl Zeiss LSM700 ZEN Acquisition Mode Acquisition a Acquisition Mode DAPI FITC
13. Live Channels Track 1 Airy Unit Fig 17 Track Acquisition DAPI FITC Smart Setup y Show all Tools AF Le cy Find Focus Set Exposure Live Z Stack Time Series Bleaching Regions 9 Laser Light Path Channels 4 Channels Tracks Channels Track 1 Track 2 ll de Select All Unselect All Snap HH 405 488 555 639 Display Fig 17 Channels 11 Carl Zeiss LSM700 ZEN RO BBR Set Exposure Gain Master Digital Offset
14. New AF Find Focus Gain Gain 1 Averaging Live Continuous Lazer is Snap 12 Carl Zeiss LSM700 ZEN Merge
15. Fig 4 Locate Acquisition Processing Maintain Fig 4 3 6 Fig 4 Fig 4 ZEN ZEN 30 24 ZEN Show all tools Show all tools Setup Manager Fig 5 Light Path
16. Dimensions Merged ON OFF boven tem Fig 20 Split Channels Channels Tracks Channels Track1 DAPI af Track 2 Ada Track 3 AS6E Splect Al Unselect All Lasers Gain Master Digital Offset Digital Gain Fig 21 oe Gain Master Digital Offset Digital Offset 0
17. Dimensions Player 1 7 2 Split Split Dimensions Merge On Off Fig 39 Split 7 3 Gallery 3D 4D A Gallery Gallery Time Series Fig 40 Gallery 24 Carl Zeiss LSM700 ZEN 7 4 Ortho Z ll kh Ortho Fig 41 Ortho hy Cut Cut
18. Set Exposure 100 Set Exposure Acquisition DAPI FITC Smart Setup Show all Tools AF ee mH Gy Find FocuX Set Exposure Live Continuous Z Stack SH Time Series Bleaching Regions Fig 18 Auto Exposure Live Fig 19 feo Ga 6 os Live Live Continuous Set Exposure
19. 0 Carl Zeiss LSM700 ZEN Microscope Objectives NE objec re opto Light Hone tives act var path Load position Control Ea TL Illumination a 10x Automatg PL i Display RL Illurnination OFF On Reflector Re Flector pto Light var path Objec Home tives Tlmmlll Load position LL ee Pos 6 Analy DIC Trans light _ Contrast Manager Display 63xJ0 75Ph2 0 DIC TL 2 04 TL Illumination On Off RL illumination On Off 5 S12 Carl Zeiss LSM700 ZEN
20. Movie 1 Compress I lt Carl Zeiss LSM700 ZEN 5 2 File Open New File Browser New File Browser File New File Browser File View Acquisition Maintain Macro Took Window Help Fig 31 File Menu Q File Browser HDD Fig 32 New File Browser Reuse
21. Axio Observer Z1 Axio Observer Z71 6 6 KBD TFT
22. 7 9 Profile Profile Spline 7 10 Information Fig 46 Profile LSM700 ZEN Fig 47 Information Carl Zeiss LSM700 ZEN 8 Jf Sh Dw all Dimensions Play Ne 3D Show all 8 1 Dimensions Dimensions Boon lela Nl ttl i Z
23. Fig 42 Cut 7 6 3D Z 3 3D Z Stack 3 3D 8 5 Series mm 2557 lt Fig 43 3D 7 7 Histo Histogram Fig 44 Histogram 295 Carl Zeiss 7 8 Co localization 2 Co localization Fig 45 Co localization
24. 2 3 1 Smart Setup 3 2 Experiment Manager 3 1 Smart Setup cs Smart Setup exten 2 Configure your experiment Fig 10 Acquisition Search Dyes Recent Recent AlexaFluor568 Alexa Fluor 488 DAPI Alexa Fluor 594 Alexa Alexa Fluor 350 Alexa Fluor 405 Alexa Fluor 430 Alexa Fluor 488 Alexa Fluor 532 Alexa Fluor 546 Alexa Fluor 555 Alexa Fluor 568 Alexa Fluor 594 Alexa Fluor 610 Alexa Fluor 633 Alexa Fluor 647 Alexa Fluor 660 Alexa Fluor 680 Alexa Fluor 700 Alexa Fluor 750 Fig 11 Smart Setup Fastest Best Signal Smartest 3 Apply Fastest Simultaneous
25. ZEN Split 500 0 PMT 1 Dye LUT y DAPI MBS T80 R20 None a a Fig 16 Light Path MBS VSD Variable Secondary Dichroic beam splitter y VSD 1nm PMT 1 SP 490 PMT 2 None PMT 1 SP 490 PMT Dyes Carl Zeiss LSM700 ZEN 2 Simultaneous Sequential
26. ON OFF ON OFF 1 2 ZEN Start System Lagin AN os a ZEN Fig 3 Start System Image Processing Image Processing Boot status Fig 3 Carl Zeiss LSM700 ZEN ZEN 3
27. 12 Live K Live ey 7s Carl Zeiss LSM700 ZEN 5 5 1 2D Imagel7 ism ec 50 ME x Fig 27 Fig 27 ZEN 2 1 ism czi
28. 2 ae Simultaneous LAL Sequential a 6 Carl Zeiss 4 LSM700 ZEN
29. Mode Line Frame Method Mean Sum Number a 4G x Carl Zeiss LSM700 ZEN Scan Area Scan Area Show al Fig 26 Scan Area BMARMBONKKRAAZALYUO RSA Ee Image Size 134 7 pm x 134 7 pm amp iPel Size 0 26 pm E im 00 lt i j 00 lt MH sows li Reset All Fig 26 Scan Area FEB A yy UTS fod BRI yI_BBOMVYAAELET
30. New File Browser LET Re use 933 Reuse am Carl Zeiss LSM700 ZEN 6 3D 3D 3D 1 Z Stack 2 3 4 Z DQ Z Stack Z Stack Bleaching Regions p gt Start Experiment Fig 34 Application Z Stack First Las
31. Fig 5 Show all tools Carl Zeiss LSM700 ZEN Show all Showall 4 Show all Fig 6 Show all ON OFF Light Path y Showil B Online Acquisition Fig 7 ZEN
32. TIFF JPEG File Export Select file name and save Save Fig 30 Export Format Data Raw data Contents of image window Ortho Full resolution image window Z single plane 1 series
33. 6 Start Experiment Z Averaging AF fest Cot coy Find Focus Set Exposure Live Continuous snap Z Stack 31 Slices Time Series Bleaching Regions Start Experiment Fig 37 Application Z 7 3 2 Carl Zeiss LSM700 ZEN 7 2D 3D 4D A Fig 38 2D 2 Z 7 1 2D 2D 3D Time Series A
34. Smart Setup Show all Tools Plan Apochromat 20 08 M27 AF e fowl Ga 0 Frame Find Focus Set Exposure Live Continuous Snap Z Stack fish Time Series __ 5 a Bleaching Regions Setup Manage Laser Light Path Acquisition Parameter Acquisition Mode Fig 24 Acquisition Mode Frame Size 512x512 Highest Highest Speed Speed Pixel Dwell Scan Time 10 8 9 7
35. _ ovl 28 Carl Zeiss LSM700 ZEN 8 5 Series 3D 3D Mie 3D 3D Fig 52 3D Series C Y 1 Render series X Y GE 2 Total frames 3 360 Difference angle Panorama jing Series 6 20 50 100 3150 0 Fig 53 Apply Player 8 3 29 Carl Zeiss LSM700 ZEN 9
36. 3 5 4 4 3 3 HBO Field Stop Aperture Stop FL P amp C ON DU RP DD Ge Carl Zeiss LSM700 ZEN 10 OFF 10 1 OFF 10 2 OFF 10 3 10 4 ZEN File Exit 10 5 Wind
37. T PMT 9 Fig 16 Gain 3 Carl Zeiss LSM700 ZEN 9 2 200um DIC DIC 0 PC 2 DIC DIC DICT 40 DICH 40
38. 1 2 3 L 4 5 6 7 8 1 2 9 10 25mm 11 12 13 LM set 14 XY 15 16 L 17 18 On Off 2 3200 19 On Off 20 TFT 21 22 23 24 25 26
39. 2 1 2 2 ON 2 Py eee ya ya vw pry Fe rarer Fig 1 Carl Zeiss LSM700 ZEN 90 405nm 440nm 488nm 555nm 639nm Fig 2 ON ON ON ON PC ON Windows LSM User
40. Best Signal Sequential Fastest Smartest Sequential Fastest Carl Zeiss LSM700 ZEN 3 2 Experiment Manager Experiment Manager Fig 13 Recent Configurations Search 1 Ex
41. 488 555 639 2 configuration Acquisition Processing HiFTta ecen DAPI FITC DAPI FITC DsRED sriment Manager DAPI FITC Fa Bimart Setup w Show all Tools AF o ml ira DAPI FITC DsRED Find Focus Set Exposure ive Continuous Stack ue Time Series Bleaching i Fig 12 Experiment Manager Fig 13 Experiment Manager x uy Show all Tools w Show all Tools New Fig 14 Show all tools Light Path Light Path Track 1 Track 4 Channel Frame Trackl Track Track3 Track4 PMT 1 5P 490 Fig 15 Light Path Carl Zeiss LSM700 ZEN Experiment Manager THER Tad Tacks Tracks
42. Microscopy from Carl Zeiss LSM Software ZEN We make it visible Carl Zeiss LSM700 ZEN ee 1 KTNN7 NT OF 2 2 6 3 7 MEA ee Ee or ess ae eee ee 11 5 18 6 3D 22 LE LN 24 8 cts ar Dg el ae a Ge en eee 27 9 rr 30 HO NKK ROR OO 36 1
43. n unter Speicher Desktop bd ed Eigene Dateien demo data arbeitsplatz 2 Jenakultur 2 Netzwerkumgebung JofBioTech_CallforPapers ED CiscoSecure Authentication Agent EDLSM 4 2 Icons a Divx Movies Movies SAxiovision 4 6 Icons Music lt Deane fm TT Dateityp SM5 kmj i Abbrechen Fig 29 Ism czi sm cz 4 Save CD LSM D 19 Carl Zeiss LSM700 ZEN 2 Export
44. ows Shut Down MY PC OFF 10 6 1 2 OFF 85 15 15 36
45. t Fig 35 1 2 terva 100 Set First Z Interval Slice gt Set First 10 00 0 00 Fig 35 Z stack 3 2 Set Last Z 4 Stop 2 72 Carl Zeiss LSM700 ZEN Smallest Y show Al i First Last Smallest Optical Slice Fig 36 Optical
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