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Contents
1. TFT Page 34 Oo ON UU BP W DN OV 0 NM DOW UU BP WN 19 20 21 22 23 24 25 26 27 Carl Zeiss Co Ltd LSM510 ZEN 2009 L2. 1 2 1 25mm LM set X Y L On Off 2 3200 On Off TFT et Page 35 Car
3. 1 Channels amp AOTF 2 Auto Exposure Gain Digital Offset 3 Gain master amp Digital Offset 4 Acquisition Mode 1 4 1 Channels Mode Acquisition Channels lt x Acquisition 4 Channels Configuration Ex405 488 555 line 2 Tri Smart Setup Show manual tools Track 1 o Track 2 ae Es Select all Unselect all Trac
4. 2D 3D 4D A 8 1 2D Fig 43 2D 2 Z Ez 2D 3D Time Series A Dimension Player 1 8 2 Split plit Dimension Merge on off Fig 44 Split 8 3 Gallery 3D 4D A Gallery
5. Reuse tae RTT PETTT PTEFT3 Fi 53 Dimension 3ch 9 2 Display AID Cr iz Fig 54 Display 9 3 Player Z 86 Prayer Ontions Fig 55 Player Page 31 Carl Zeiss Co Ltd LSM510 ZEN 2009 9 4 Overlay View Overiay Fig 56 Overlay
6. Fig 7 Show all on of Light Path Se a Online Acquisition Fig 8 ZEN
7. Save Page 22 Carl Zeiss Co Ltd LSM510 ZEN 2009 II I Export TIFF JPEG etc Tagged Image File File Menu Export Contents of image window single plane Raw cate eres contents of tiage vindow sngle plane Select file Contents of image window senes eae it name and save REO Fig 33 Export Format Data Raw data
8. Page 21 I Ism Fig 31 ZEN peichern unter Speichem i Desktop Ff FE Eigene Dateien demo data i Arbeitsplatz enakultur Netzwerkumgebung JofBioTech_CallforPapers a CiscoSecure Authentication Agent CLSM 4 2 Icons Rl bivx Movies Movies 2 Axiovision 4 6 Icons music Is Datemname Image 7 Dateityp SMO etn ee Abbrechen Fig 32 File Carl Zeiss Co Ltd LSM510 ZEN 2009 File Menu Save Save as ZEN eR Fig32 2 Fig3273 2 Save Save Zeiss LSM lsm
9. Focus eS ep mall TT a 2 8 LO Closed 100 96 1 EE Fig 12 Axio Observer Z1 Fig 14 laa CPI Dc tt Fig 13 Page 9 Carl Zeiss Co Ltd LSM510 ZEN 2009 4 II Configuration LSM
10. Windows Zeiss ZEN Light Edition http www microimaging zeiss co jp I Export 2 lsm C D LSM5 D
11. n 85 15 4 ECU
12. 5 MO Carl Zeiss Co Ltd LSM510 ZEN 2009 1 LRIEFLORBSBLIT BROANZ UV 1 1 Box 1 2 1 3 1 4 1 1 E Box 1 ON E Box
13. 2 Sectioning Scan 3 a Continuous Snap 21 Slice s Z Stack Bleaching Regions gt Start Experiment Z stack First Last Fig 39 Application Z Center or ee eer Set Last ES 21 a Keep Interval Slice Set First Z SetFrst 2063 lt Fig 40 Z stack Set Last Z
14. Ze ZEN 2007 Light Edition 0 ZEN 2007 Light Edition Pile Edit Window Help File Edit Window Help n Open 1 Dave Fig 36 File Menu Fig 37 File Open 2 xi le Biomed lt amp e Ef Biafilm lsm ff CRmouseFITGTRITG sm A BioMed mdb Maus DIC Haarzelle m CytaskeletonDNA sm MDCKcells lsm DAPIeCF PAlexa546 lsm DaPleGFPAlexa546 lsm drosophilaprojektion lsm ICytoske letonDNA lsm 0 Fig 38 File Page 25 Carl Zeiss Co Ltd LSM510 ZEN 2009 7 3D BOER DUT Z 1
15. 4 LSM File Exit Start Exit 5 Windows Start Shut Down PC OFF 6 OFF n 8 596 15 15 Page 40
16. LSM Fig 14 LSM Configuration Fig 13 Recent Configurations Search Ex 488 1 Ex 488 555 639 2 Pesca rates iis ee Acquisition Configuration Ex405 486 555 fine Smart Setup Show manual tools a ruk Auto Exposure Live Continuous Snap Z Stack e Time Series e Bleaching gp oe e ci 7 Fig 15 Configuration Fig 16 Configurations
17. Imaging Setup Light Path AA Imaging Setup Showall Mode Channel Mode simultaneous _ Simultaneous Sequential Switch track every Frame w Track Track Fig 17 Imaging Setup Page 10 Carl Zeiss Co Ltd LSM510 ZEN 2009 Configuration gr aire Loh Ghennel Moda Lambeds Mode Onine Fimgenonnanag Imaging Setup Light Path Imaging Setup Track Light Path Fig 19 Light path HFT PMT Page 77 Carl Zeiss Co Ltd LSM510 ZEN 2009 4 IV Scan Control
18. Histogram Fig 49 Histogram Page 29 Carl Zeiss Co Ltd LSM510 ZEN 2009 8 8 Co localization 2 Co localization 8 9 Profile Profile Spline 8 10 Information
19. 1 2 0 PC 2 DIC TI DIC DICT 40 DICH 40 1 DIC DIC DIC LSM Page 37 749 Carl Zeiss Co Ltd LSM510 Z
20. 10 8 9 7 Acquisition Mode Bit Depth 16bit 65 536 12bit 4096 8bit 256 Page 16 Carl Zeiss Co Ltd LSM510 ZEN 2009
21. Stop LET JY Showal Page 26 Carl Zeiss Co Ltd LSM510 ZEN 2009 Optimal Z cK SF Center Optimal Optical Slice Fig 41 Optimal Start Experiment Z Average New Auto Exposure Live Z Stack 21 Slice s Time Series Bleaching Regions Fig 42 Application Z 8 wy Showa Set Last co G Continuous Snap gt Start Experiment A Page 27 Carl Zeiss Co Ltd LSM510 ZEN 2009 8
22. gt On Off On Off Page 7 FAA Carl Zeiss Co Ltd LSM510 ZEN 2009 1 2 A SYSTEM PC COMPONENTS ON SYSTEMPC ON 1 3 ON ON a Ws 1 4 ON 2 ON Windows Windows
23. Contents of image window Ortho Full resolution image window Single plane 1 Series Movie 1 Compress Page 23 Carl Zeiss Co Ltd LSM510 ZEN 2009 I I File Browser ZEN File_ Browser ZEN Ctrl F File Menu F
24. Workspace Configuration 2 Page 6 Carl Zeiss Co Ltd LSM510 ZEN 2009 4 REARGIS LSM510 1 I IV Acquisition Configuration SmartSetup W Show manual tools New Auto Exposure Live Continuous Z Stack Time Senes Bleaching Tile Scan Regions Setup Manager Laser f Imaging Setup Light Path Online Acquisition Acquisition Mode Channels Focus gt Stage Multidimensional Acquisition i Information On Experiment Fig 9 Acquisition Page Carl Zeiss Co Ltd LSM510 ZEN 2009 4 I Lase
25. El 5 Page 13 Carl Zeiss Co Ltd LSM510 ZEN 2009 Gain Master Digital Offset 3 1 Gain Master amp Digital Offset Auto Exposure 100 Gain Master Digital offset 1 Acquisition Acquisition Channels Stop aa Channels Showall Tracks OM Track 1 Ww Track 2 Select all Unselect all Lasers Fiq 22 Channels Gain Digital Offset Master Gain Digital O
26. Fig 50 Co localization Fig 51 Profile Fig 52 Information Page 30 Carl Zeiss Co Ltd LSM510 ZEN 2009 9 IS US REA lr 3D Show all 9 1 Dimensions Z Zoom Channel s Merge Single on off a Crop
27. Angle Delete Cut region Coordinate Z Hide Number Measure ovl Page 32 Carl Zeiss Co Ltd LSM510 ZEN 2009 9 5 Series 3D 3D go 3D 3D Fig 57 3D Series CX Y
28. S Acquisition Mode Objective Plan Apochromat 63x 1 4 Oil DIC Scan Mode Frame Frame Size Speed Scan Area Fig 26 Aquisition Mode Acquisition Mode Averaging Fig 26 Mode Line Frame Method Mean Sum Number Snap Page 17 Carl Zeiss Co Ltd LSM510 ZEN 2009 5 Sequential Multi Track CH GERAIS LSM5 Simultaneous Single Track Sequential Multi Track 2 A i Rhode BS Gl SS Sec
29. Page 18 Carl Zeiss Co Ltd LSM510 ZEN 2009 Multi Track ito ko Marge p Dimension Overlay on off Fig 27 Split Gain Master Digital Offset Master Gain amp Digital Offset HAIL Track 1 Track 2 Channels Showall Select all Unzelect all Track 1 al 405 463 355 635 fy 450 nin Fiq 28 Channels Section Page 19 Carl Zeiss Co
30. 1 Render series X Y GE 2 Total frames 3 360 Difference angle Panorama 30 Series Fig 58 Apply 6 Player Player 9 3 Page 33 Carl Zeiss Co Ltd LSM510 ZEN 2009 70 HEF SAGE LSBE IL GAG Axio Observer Z1 FO FSA RCL CHAE ma FY He Ya LSM ON Axio Observer Z1 6 6
31. Dimensions Ch Fig21 Range Indicator ch1 T1 Ch2 T1 Ch1 12 Fiq 23 Dimensions Range Indicator 0 Detector Gain Amp Offset Fiq 24 Ranae Indicator 1Airy Fig 20 Detector Gain 1000 1100 Fig 22 Channel
32. Boot status c Page 3 Carl Zeiss Co Ltd LSM510 ZEN 2009 ZEN Fig4 5 ZEN A C D E 3 F G Fig4 ZEN 6 7 8 9 10 Fig5 ZEN Page 4 Carl Zeiss Co Ltd LS
33. Page 8 Carl Zeiss Co Ltd LSM510 ZEN 2009 4 IL Microscope Setup Ocular Online Fluorescence Shutter On Shutter Off Fig 11 5 eae ae PsReg Da on of ame os
34. Gallery Fig 45 Gallery Page 28 Ortho Z 7 im 8 5 Carl Zeiss Co Ltd LSM510 ZEN 2009 Ortho Fig 46 Ortho Cut Z Z 3 3D My ad Curt Cut 8 6 3D Z 3 Fa47Cut Bi 3D 9 5 Series I Fig 48 3D Histo 8 7
35. OK ON OFF REMOTE CONTROL ON OFF Page Carl Zeiss Co Ltd LSM510 ZEN 2009 2 FAL FALTI STI OREB LMF Fig1 ZEN 2009 Fig2 ZEN Start System Login ZEN 2009 Laser Scanning Microscope LSM 700 O Beetsiatus Start System Image Processing Fig3 Start System Image Processing
36. Fig 22 Intensity Page 15 Carl Zeiss Co Ltd LSM510 ZEN 2009 4 Acquisition Mode Acqguisition Acquisition Mode ws Acquisition Mode Objective Plan Apochromat 63x 1 4 Oil DIC Scan Mode Frame Speed Pixel Dwell 1 60 usec ScanTime 3 93 sec Averaging Number Bit Depth 8 Bit Scan Area gt Fig 25 Acquisition Mode AxioSkop2FS Optimal Frame Size 512 x 512 Optimal N A Acquisittion Mode Speed 1
37. Ltd LSM510 ZEN 2009 6 HHAGORR HA ORFERAHL 2D Z Time New Fig 29 Fig 31 Fig 30 Page 20 Carl Zeiss Co Ltd LSM510 ZEN 2009 LSM5 2 I Ism
38. EN 2009 1 Ph1 3 Ph Ph 1 2 Page 38 Carl Zeiss Co Ltd LSM510 ZEN 2009 ON 1 2 3 4 HBO Field Sto
39. M510 ZEN 2009 3 VAIKDIPORBAETE ZEN 3 Fig3 Fig3 3 Fig6 ZEN ZEN 30 24 ZEN Page 5 Carl Zeiss Co Ltd LSM510 ZEN 2009 Da Showal 7 Show all
40. Microscopy from Carl Zeiss LSM SFamily LSM Software ZEN 2009 ZO We make it visible Carl Zeiss Co Ltd LSM510 ZEN 2009 LSM510 1 2 3
41. a tees I es 1 t eo H lt oa 2 ia L Simultaneous Single Track Rhodamine FITC nh a Fitc Em Litt tr 9 wf Sequential Multi Track GIk 1 Line Frame Simultaneous Single Track Configuration Find amp
42. ffset Digital Gain Digital Offset Transmission 488nm 5 10 Page 14 Carl Zeiss Co Ltd LSM510 ZEN 2009 Range Indicator Master Gain Digital Offset 3 2 Range Indicator View Dimension
43. igital Offset Acquisition Configuration Ex405 488 555 line s Online Acquisition Acquisition Mode Channels Focus Multidimensional Acquisition i Information On Experiment Fig 21 Auto Exposure Live Continuous Auto Exposure Master Gain Z Master Gain 1 Average Live Continuous
44. ile Browser Explorer Image Database ZEN File Browser File New File Browser ZEN ZEN 2007 Light Edition File Edit Window Help Fig 34 File Menu File Browser Open G Rel 3 2 Sample HDD Fig 35 File Browser Page 24 Carl Zeiss Co Ltd LSM510 ZEN 2009 T W 1 File Open
45. k 1 ww 405 488 555 639 488 nm Muitidimensional Acquisition i Information On Experiment Fig 20 Channels 1AU 1 Airy Units Page 12 Carl Zeiss Co Ltd LSM510 ZEN 2009 Image acquisition 2 Auto Exposure Detector Gain Amp Offset Auto Exposure LIVE Continuous Snap Stop LIVE Continuous 3 P 6 a 5 Gain master D
46. l Zeiss Co Ltd LSM510 ZEN 2009 10 I 1 Ocular 2 TFT HAL on off ON 3 H 3 LD N OF WwW B WN Page 36 Carl Zeiss Co Ltd LSM510 ZEN 2009 10 I 200um DIC DIC
47. p Aperture Stop FL PEC ON DU BP Page 39 Carl Zeiss Co Ltd LSM510 ZEN 2009 11 VRTF LOE T BIR OOFF 1 OFF Setup Manager Laser Off Blue Diode 405 405nm standby off Argon 2 458 488 514nm standby Output off off 5 5 HeNe 543nm 594nm 633nm off 2 OFF 3
48. r Control Setup Manager Laser _ J r Laser Laser Laser Lines nm i Argoni2 458 477 488 514 Carvin ous L HeNe5o4 504 Heie5 As 544 amp HeNeos3 633 7 Laser Properties Setup Manager Lew Maximum Power 30 0 mWW Wavelenath 456 477 468 574 nm dg hw o LiHHE Path Onin Mu otatus Wanming up Soquenion Winds i Chaneels Tube Curent 359A Output 5 oa Pecuk Be i uhm oral Acquistion Infommabon Unk lil Fig 10 Blue Diode 405 405nm standby Laser Properties Status Warming up Ready on Argon 2 458 477 488 514nm standby Laser Properties Status Warming up Ready on Output 50 HeNe 543nm 594nm 633nm on 10
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