LSM5 Family
Contents
1. Single Track Configuration Find amp Page 21 Carl Zeiss Co Ltd LSMS5EXCITER ZEN 2007 Multi Track Acquisition Imaging Setup Multi Track a Mode Channel Mode Switch track every Frame Configuration yv W trada Ch2 Single Tra2. TFT Page 31 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 L 1 2
3. Camera CCD LSM Fig10 Ocular Setup manager Light path Light Path S amp Light Path Configuration ow wm x I to030M7 E
4. 0 PC 2 DIC Ii DIC DIC I 40 DICH 40 1 DIC DIC DIC LSM Page 34 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 1 Ph1 3 Ph
5. ZEN 3 Fig4 D Fig571 Fig571 overlay 3
6. ZEN 30 21 x 2 amp Time Series Pro Mode i Interval Cycle Delay Time Cycle Delay Time experiment 1 Basic Mode Time Series Marker Cycles Marker not defined Interval Ee Ea Ca Cap Fig6 ZEN Page 5 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Fig6
7. Aperture 0 3911 ry BF on off Fig11 AxiolmagerZ1 Page 10 Carl Zeiss Co Ltd LSMSEXCITER ZEN 2007 amp Light Path FSet02 wf waa wae wee LE Closed 100 63 gt EC Plan Neofluar 10x 0 30 M27 Aperture 0 3911 ry BF RHKAERORYT ILS RE X cite 120 Configuration DRKI1IVA ODKAVEDYYIL ERO or HBO100 mat Otte 4961 gt Fig 1 2 so toi Si ol 0 EB CE 5 a Fc al 2 UB CE BAL TC TA RE Reflector Turret 3477 JL4 SH BEBE O RIFEMFUHIL fig13
8. Load Save wa Light Path Ocular Configuration Fig13 ED ss Page 11 Carl Zeiss Co Ltd LSMS5EXCITER ZEN 2007 4 I1 Configuration LSM 2 Single 77acK Multi 77ac 5 Multitrack OF CEERORNAFHALETF LSM
9. fig30 Page 24 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 LSM5 2 I ism Windows Zeiss Image Browser http www zeiss de ImageBrowser I Export 2 lsm
10. Workspace Configuration 2 on off Page 6 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Page 7 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 4 Single Track Simultaneous CHGS LSM5EXCITER I I IV Acquisition Continuous Setu
11. Detector Gain Fast Continuous 1 Average La Find GS Detector Gain amp Amp Offset Page 17 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 4 IV 2 amp Detector Gain Amp Offset Find
12. I I File Browser ZEN File_ Browser ZEN Ctrl F File Menu File Browser Explorer Image Database ZEN File Browser File New File Browser EN ZEN 2007 Light Edition File Edt Window Help Save Save As New File Brower Fig34 File Menu File Browser
13. yy Light Path Acquisition Fig7 ZEN Light Path ZEN
14. 90 Ar 458 488 514nm B DAr Power Enable ON 90 Standby on Power DEAS 9 10 LED LED 2 es a 1 PC 2 3 PC 2 10 Page 2 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007
15. Fig14 LSM Single Track Acquisition Imaging Setup Single Track HH Imaging Setup configuration SMM 22S RARRZGEMRRENET OT EE Mode Channel Mode Switch track every Frame YY Tracki Ch2 Fig15 Imaging Setup Imaginq Setup Configuration Single Track Alexa 488 Cy3 se 1 Alexa 488 a Mae Multi Track Multi Switch track every duo seq wr ee Load KEVERS YF ose 1 ose 2 lasers on Ch2 test meta z stack config Fig16 Page 12 Carl Zeiss Co Ltd LSMS5EXCITER ZEN 2007 Store Delete
16. Fig4 5 ZEN A B C D E 3 F G Fig4 ZEN 6 7 8 9 10 Fig5 ZEN Page 4 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 3 VIKDL FP OBRARlE ZEN ZEN LSM5
17. C D LSM5 D I Ilsm File Menu Save Save as ZEN fig30 2 ca fg3073 Save EI Fig31 ZEN Page 25 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Save Speicher unter
18. Ocular 2 TFT HAL on off ON 3 H 3 1 2 3 4 6 LD 7 53 Page 33 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 200uum DIC DIC AK 1 2
19. Open G Rel 3 2 Sample HDD CR mouse FITC TRI MDCK cells Fig35 File Browser Page 27 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 I 1 File Open EN ZEN 2007 Light Edition File Edit Window Help Ze EN 2007 Light Edition File Edt Window Help o 2 Fig36 File Menu Fig37 File Open 6 x lo Biomed lt amp ex Ee lt a Biofilm ism Sf IC RmouseFITCTRITG Ism A BioMed mdb Maus DIC Haarzelle m CytaskeletonDNAsm MDCKcells lsm DAPIeCF PAlexa546 lsm DaPleGFPAlexa546 lsm drosophilaprojektion lsm ICytoske letonDNA sm 0 Fig38 File Page 28 Carl Zeiss Co Ltd LSM5EXCITE
20. Transmission Excitation AOTF 488nm 5 lt 109 Page 18 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Range Indicator Detector Gain Amp Offset View Dimension View Dimensions Ch fig22 Range Indicator Meas _ ia fia22 View Dimension Position 4 Range Indicat
21. FL P amp C oo ND FB OO NO Page 36 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 9 LRTIFLORTS BIOOFF 1 OFF HeNe543 HeNe633 WD Ar Standby OFF OFF 5 5 Power Enable OFF 2 OFF 3 LSM File Exit Start Exit 4 Windows
22. Ph 1 2 Page 35 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 8 TV ON 1 2 gt 3 4 HBO Field Stop Aperture Stop
23. Saturation 100 Detector Gain Ampl offset Acdquisition Acquisition Channels Stop FP S Channels Detector Gain Amp Offset Channel Ch2 Pinhole Gain Amp Offset Intensity0 Transmission AOTF Excitation Gain Amp Offset Amp Offset
24. rd Fig1 ZEN 2007 ZEN ZEN 2007 Fig2 ZEN Start System Login ZEN 2007 HD Laser Scanning Microscope LSM 510 Boot rtm Start System Image Processing Login ZEN 2007 Laser Scanning Microscope LSM 510 Boot Status CP DIOs CP Video Switches CP Cameras a CP HRZ CP Focus bed CP Focus Start System Offlime Demo Fig3 C Start System Image Processing Boot status c Page 3 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 ZEN
25. Page 23 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 6 HRIRORA RORPFEREHL a Single a Start 2D Z Time New 7 oe ont Or re 0o Or oy oOo 0 Mae 0 0 Oe OF oO 7 Fig29 none eee fig 30
26. 10 25mm 12 13 LM set 14 X Y 15 16 L 17 18 Bik On Off 2 3200 19 On Off ARAL 20 TFT 21 22 23 24 25 26 SEE SE OA BPW NYO Ke Page 32 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 8 1 1
27. Detector Gain Amp Offset Multi Track Detector Gain amp Ample Offset we Channels Chann Ch Pinhole i 76 0 lt 1 00 Airy mC omen gt Um Max Gain 78 Amp Offset 0 10 Track Track 458 477 488 514 561 633 800 Status Transmission 488 nm t k 633nm fig28 Channel Gain amp Amp Offset Section Single
28. Start Shut Down PC OFF 5 OFF 659 309 5 15 Page 37
29. Select file name and save Fig33 Export Format Data Raw data Contents of image window Ortho Full resolution image window image window 1024 x 1024 Single plane 1 Series Movie 1 Page 26 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Compress
30. Page 16 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 4 W 1 FindRAVA E5 Image acquisition Find Fast Continuous Single Stop Fast Continuous Detector Gain Amp Offset Continuous Setup Manager Laser 4 amp Imaging Setup EE Light Path Online Acquisition gt Acquisition Mode 4 Channels Focus gt Stage 4 Regions Multidimensional Acquisition ZStack 4 Bleaching 29 Time Series Tile Scan 2 i Information On Experiment Fig20 Image Display Detector Gain Z Continuous
31. Acquisiton Mode Bit Depth 8bit 256 12bit 4096 2 Acquisition Mode Average Fig18 Line 4 KI ALYY I ORE BIXDUYT Mode Line Frame Method Mean Sum Number Page 15 Carl Zeiss
32. Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 4 IV Scan Control 1 Acquisition Mode 2 Channels amp AOTF 1 2 1 Acquisition Mode Acqguisition Acquisiton Mode w Acquisition Mode Objective Plan Apochromat 63x 1 4 Oil DIC Scan Mode Frame Frame Size Speed Pixel Dwell 1 60 usec ScanTime 3 93 sec Averaging Multidimensional Acquisition Number Bit Depth 8 Bit ZStack 2 4 B
33. UV 405nm DAPI B FITC Cy2 G Rhodamin Cy3 R Cy5 Setup Manager Light Path Light Path fig17 E Light Path Acquisition LSM None 0 0 Mirror NFT 565 BP 650 710 IR ao IR vo L ms 48815616 Cd em 1 Laser r None L Stage Focus FT 510 LP 515 Ch D Fig17 Single Track Light pathn LSM LP BP Page 13 HFT NET
34. 3 08 pm Set Last 20 241 Set First 25 695 Range 45 94 um Focus Position 2 73 Hm Ww Z Stack Navigation Set First Z amp Pcie Center Set Last Z Stop ee ene Z stack Navigation Page 29 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Sectioning Scan 6 Optimal Interval Z Optical Slice Optimal Interval 3
35. Co Ltd LSMS5EXCITER ZEN 2007 2 Channels Mode Acquisition Channels w Channels Channel Ch2 Pinhole 1 00 Airy Units 116 pumsection Gain Amp Offset 2 amp Imaging Setup DF Ught Path Track Track1 Online Acquisition v v 458 477 488 514 561 633 2 Acquisition Mode 4 Channels Focus Status Transmission Regions Multidimensional Acquisition Z Stack Bleaching 488 nm 633 nm 4 Time Series 2 Tile Scan 2 i Information On Experiment gt Fig19 Channels 1 Airy Fig19
36. 27 pm Z D Start Z Average Z ZEN LE Page 30 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 8 HEF AE KOBE PILE GAG Axio Observer Z1 LSM ON Axio Observer 1 6 6
37. 510META Blue Diode 405 405nm standby 5 on Argon 2 458 477 488 514nm standby 5 on Output 509 HeNe 543nm 594nm 633nm on 10 off off Page 9 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 4 II Microscope Setup Ocular Camera LSM Ocular
38. BY Speichem Desktop 7 ey EA Eigene Dateien demo data Arbeitsplatz 9 Jenakultur oe ee Zeiss LSM EE lsm A ll gt Dateiname Image2 22 ol SS lt _Abbrechen Save Fig32 File II I Export TIFF JPEG etc TT HSE AE File Menu Export Contents of image window single plane amp ct sino VC Re Select file Eee eee name and save
39. Microscopy from Carl Zeiss LSM5 Family LSM Software ZEN 2007 We make it visible Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 1 LRIFLORMELIT ZBROANFZA 1 2 On 3 PC 4 Ar HeNe 1 2 ON a 3 PC Windows Log in 4 HeNe 543nm G HeNe 633nm R ON Page 1 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 405nm V
40. R ZEN 2007 7 3D HR OERIZDUT Z 1 2 Sectioning Scan 3 Multi Channel Acquisiton JS Ase Z Stack Mode Mark Mode W First Last Center First Last Number of Slices Interval Continuous Keep Interval Slice Optimal Interval
41. bjective Plan Apochromat 63x 1 4 Oil DIC Scan Mode Frame Bo X 512 au Y 512 gt B Optimal speed gt Pixel Dwell 1 60 psec ScanTime 3 93 sec Averaging Number Bit Depth 8 Bit Scan Area fig24 Aquisition Mode Single ca 4 2 5 Multi Track Sequential CH zRS Page 20 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 LSM5 Single Track Multi Track 2 A RidB a 5 1 4 m 7 oe 9 fg Single Track a Een Rhodamine FITC RodEx RhodEm Multi Track 1 DOE SET Line Frame
42. ck Mode Poe ee Multi Track Multi Alexa 488 Cy3 Switch track every duo seq Imaging Setup ae Load KELME Dy Y Tads ee Ch2 ose 1 ose 2 lasers on z stack config fig25 Store Delete Find Multi Track Find Marge fia26 2D Page 22 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Marge Dimension Overlay on off fig27 Split
43. leaching 7 ow ew sas z Scan Area Tile Scan i Information On Experiment Fig18 Acquisition Mode AxioSkop2FS Optimal Frame Size 512 x 512 Optimal N A Page 14 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Acquisition Mode Speed 1 10 8 9 7
44. or 0 Detector Gain Amp Offset fia23 Ranae Indicator 1Airy fig21 Detector Gain 1000 1100 fig 21 Channel fg21 Intensity Page 19 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 Acquisition Mode w Acquisition Mode O
45. p Manager 4 Laser 4 amp Imaging Setup DE Light Path Online Acquisition 2 Acquisition Mode 4 Channels Focus gt Stage Regions Multidimensional Acquisition 4 Z Stack Bleaching 29 Time Series qd Tile Scan 4 i Information On Experiment Fig8 Acquisition Page 8 Carl Zeiss Co Ltd LSM5EXCITER ZEN 2007 4 I Laser Control LSM5 Exciter CLF COL Y FRILGLVFF Ki CORELITUF LA Setup Manager Laser LSM5107510META DPSS 561 10 Chameleon 720 930 HeNe633 633 Continuous 4 Laser Information Setup Manager J Laser 4 amp Imaging Setup 0 F Light Path Maximum Power 30 0 mW Online Acquisition Acquisition Mode Channels Focus lt gt Stage lt Regions Wavelength 456 477 466 514 nm Status Warming up Tube Current 560A Output gt Fig9 x LSM510
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