福岡県で分離された稀な血清型の志賀毒素産生性大腸菌について[PDF
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1. 25 Polymerase Chain Reaction RPLA RIC k OER BR PETE ER E aprA SI a eaeA hlyA FTE E 1 60600 1 0 a EE a O8 H16 1 2 063 H6 1 2 Si ge 091 H14 1 1 O91 H14 1 1 091 H21 S 1 1 a 1 1 i 2 1 0103 H2 3 1 se el 1 1 1 fs 4 O103 HI1 1 1 E A a 0115 H10 1 1 O118 H16 1 1 es eal es 0119 H7 1 1 O121 H14 1 2 ei ie ae O150 H11 1 1 Ao 0165 H14 1 1 1 1 0165 NM 1 1 2 4 OUT NM 1 2 NM OUT O 091 H21 ege4 LAL eaeA 1 Scheutz F et al APMIS Acta Pathologica ege4 ege4 1 091 3 JEF zzi A A a2. O 1 9 818 0135 39 O H 2xYeast Extract Tryptone 2xYT Becton Dickinson and Company
3. VTEC RPLA H Ly f Polymerase Chain Reaction PCR si 2 stx2 1 BRRR eze4 my4 DNA LB 25mM L 95 C54 Thermal Cycler SP stxi stv e
4. sz ste Stx2 Stx2vha Stx2vhb Stx2vpl O63 H6 stro 063 He y4 ege4 ege4 aces ege4 16 10 NM SHE SAUTE 7 1 ege4 Beutin 0157 89 40 eaeA NR Wang 5 HUS 6 TE
5. 091 CT SMAC 16 6 37 5 16 31 3 O118 H16 2007 ege4 fam RIERREN O BA FI ES Lae WEE 5G AE i FR 3 4 7 8 9 10 11 12 13 Microbiologica et Immunologica Scandinavica 112 9 569 584 2004 29 117 128 2008 Paton AW amp Paton JC J Clin Microbiol 36 598 602 1998 Yang JR et al J Clin Microbiol 45 11 3620 2625 2007 Scheutz F et al Bergey s Manual of Systematic Bacteriology 2nd ed vol 2 Part B 607 624 2005
6. 4 4 2008 MacConkey Agar Base Becton Dickinson and Company a L Rhamnose Monohydrate L Sorbose 1 Rhamnose MacConkey Sorbose MacConkey Luria Bertani LB HE Becton Dickinson and Company IZ L 3541 C 2242 Bu z 1 O15 Ri RA ip Sa DEA E 7 026 0111 O 2 2 1996 4 2008 1 1 AD 23
7. 35 59 64 2008 1 1996 2008 12 0111 O HF 1 23 7 4 15 4 874 FRO 9 5 0157 026 R 23 16 2 O H F O91 ege4 hlyA
8. 3 0157 026 0111 O JEF CT SMAC Rhamnose ME TE SRE 01 HI 1 EG ar T 08 H16 1 063 H6 4 091 H14 2 091 H2 4 BACT RFR 0103 H2 4 0103 H11 0115 H10 0118 H16 0119 H7 0121 H14 en E 0150 H11 1 im 0165 H14 2 0165 NM 1 OUT NM 1 NM aa x OUT O kk k IROZ sue 3 Sorbitol 1mm Rhamnose MacConkey Rhamnose O103 H11 1 O118 H16 1 O130 H11 1 O165 NM 1 OUT NM 1 18 Sorbose MacConkey Sorbose O1 H1 1 BR 063 H6 1 O103 H11 1 O121 H14 1 O130 H11 1 0157 0157 O8 H16 1
9. eaeA 0O O91 CT OXOID CT SMAC ID BR AR FERE L uremic syndrome HUS FE t 5 0157 026 O11 181 ie 0157 026 0111 2 Hemolytic 2004 O O 1996 MA O
10. Lactose Sucrose Sorbose NI A ity T ai r 2 RN _ We at 32 a a ao aot aot A 1 gt x R O119 H7 1 10 SM 3 4 Stx Stx 5 stx ste O16S NM DAT O8 H16 O121 H14 OUT NM su sti ecge4 hlyA O103 H2 4 O103 H11 1 O165 NM 1 O118 H16 1 O121 H14 1 0150 H11 1 OUT NM 1 hlyA eae4 O1l H1 1 O91 H14 1 O91 21 3 0165 H14 2 eaeA hhA O63 H6 1 O63 H6 RPLA Stx2 1 16 1 32 Stx2
11. 3 2xYT 35 1 22 2 2xYT 1 v H 1 22 H 3 100ul 1 23 1 v v 0 5ml 50 1 H 2 4 Becton Dickinson and Company 12 ABPC
12. 0157 026 0111 O 2 23 DHL GRA 2 23 Sorbitol MacConkey Agar OXOID O157 TSI SIM C LIG KL 35 1 CT 2242 Becton Dickinson and Company B 2 3 O LB LB EREEREER L121 15 3000rpm 20
13. CP CPFX CTX FOM GM KM NA SM ST FAI ST TC TMP 23 PRO TRA BES EEE Tryptone Soya Broth TSB OXOID 35 1 CC 2 8 No 0 5 Becton Dickinson and Company 12 3541 CT 2242 2 5 TARR Stx Reverse Passive Latex Agglutination RPLA VTEC RPLA Casamino Acid Yeast Extract CAYE 35 1 16 20 3000rpm 20
14. 3 3 4 23 10 12 SM O91 H21 2 O103 H2 1 O150 H11 1 T SM TE 2 O165 NM 1 O91 H21 1 O115 H10 1 OUT NM 1 SM TE ABPC 3 O118 H16 1 SM TE KM 3 4 23 itd Al SM O91 H21 1 O91 H21 1 O103 H2 1 O150 H11 1 SM TE O16S NM G O91 H21 1 O115 H10 1 OUT NM 1 SM TE ABPC 0118 H16 1 SM TE KM O119 H7 1 SM TE ABPC KM NM OUT O 0157
15. o 6 23 0111 O O91 1 3 SAB z we 0103 2 Ore Heri O1 063 0118 0119 0121 0150 OUT 1 O8 1 0115 1 3 2 3 DHL 23 23 6 3 O91 FEY 1 0115 091 CT SMAC 091 091 H14 2 O91 H21 4
16. 49 8 517 522 1996 Hussein HS J Anim Sci 85 E63 E72 2007 Beutin L et al Appl Environ Microbiol 73 15 4769 4775 2007 Frank C et al Vector Borne Zoonotic Dis 2008 Stephan R et al J Dairy Sci 91 7 2561 2565 2008 Seto K et al J Vet Med Sci 69 12 1215 1222 2007 Beutin L et al Emerg Infect Dis 4 4 635 639 1998 Wang G et al J Clin Microbiol 40 10 3613 3619 2001 2 Minor serogroup Shiga toxin producing Escherichia coli isolated in Fukuoka prefecture between 1996 and 2008 Sachiko NAKAMURA Yoshiki ETOH Mitsuhiro HAMASAKI Koichi MURAKAMI Shigeyuki TAKENAKA Kazumi HORIKAWA Fukuoka Institute of Health and Environmental Sciences Mukaizano 39 Dazaifu Fukuoka 818 0135 Japan Minor serogroup Shiga toxin producing Escherichia coli except O157 O26 and O111 isolated in Fukuoka prefecuture between 1996 and 2008 were tested about biochemical characteristics and antibiotics susceptibilities and investigated about pathogenic factors There were 23 strains that were isolated from 16 patients including two food handlers and seven asymptomatic individuals including four food handlers They were classified to 15 serogroups All strains isolated from food handlers were O91 Not all patients had eaeA or hlyA even with bloody diarrhea Key words Shiga toxin producing Escherichia coli minor serog
17. 7 0121 0103 091 6 23 O91 O103 47 8 a ae a 0121 1 O118 Stx1 O118 H16 9 2000 2007 a 1 1 0157 026 0111 Ei UE ELEL KO O O91 0103 0165 Ol O8 063 O115 O118 O119 O121 0150 OUT 14 ena 3 1 0157 026
18. 3 2 1 O119 H7 1 O163 H14 2 OUT NM 1 Seto V O9l H14 1 1 CT SMAC O157 O26 0111 CT SMAC O91 HUS Moo MS CT SMAC O63 H6 1 0165 NM 1 Sorbitol N oy
19. 26 0111 90 1998 Lin 2B L EA H16 1 4 0 1 O121 H14 1 2 tall ACRES EET STAT ICHLA S AU 7c Se PEEVE KI MAA amp PRB EEE iL li O157 O26 Olll O91 O165 O103 O150 O115 O119 Ol O8 O121 063 O118 OUT 1996 25 25 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1997 50 32 15 3 0 0 0 0 0 0 0 0 0 0 0 0 1998 40 33 4 3 0 0 0 0 0 0 0 0 0 0 0 0 1999 94 30 54 6 2 2 0 0 0 0 0 0 0 0 0 0 2000 110 100 8 0 0 0 1 1 0 0 0 0 0 0 0 0 2001 37 35 0 0 0 0 1 0 1 0 0 0 0 0 0 0 2002 69 56 12 0 0 0 1 0 0 0 0 0 0 0 0 0 2003 116 102 12 0 1 0 0 0 0 1 0 0 0 0 0 0 2004 106 95 4 3 2 0 0 0 0 0 1 0 0 0 0 1 2005 56 51 2 1 1 0 0 0 0 0 0 1 0 0 0 0 2006 74 58 11 4 0 0 0 0 0 0 0 0 1 0 0 0 2007 97 77 9 6 0 1 2 0 0 0 0 0 0 1 1 0 at 874 694 131 26 6 3 5 1 1 1 1 1 1 1 1 1 OUT O O150 H11 1 O165 H14 2 O165 NM 1 i CO OUT NM 1 NM 2 2000 2007 0 0157 O26 0111
20. aeA hlyA Paton 95 C 2 94 C 50 62 40 35 1MTris HC1 1 Jo 1 PCR 72 3 PCR 2 WW Le EE sti sto it ie Primer Stx1 EVT 1 stx1F stx1R stx2F stx2R eaeAF eaeAR hlyAF hlyAF 2 Stx2 Di z JL PCR 05 3 ATAAATCGCCATTCGTTGACTAC AGAACGCCCACTGAGATCATC 1 SIX 180 GGCACTGTCTGAAACTGCTCC TCGCCAGTTATCTGACATTCTG SIX 255 GACCCGGCACAAGCATAAGC CCACCTGCAGCAACAAGAGG eacA 384 GCATCATCAAGCGTACGTTCC 534 AATGAGCCAAGCTGGTTAAGCT EHECAl
21. roup regular fecal examination of food handlers 091
22. yA 3 3 1 1996 2008 2 0157 E Hy 1996 O157 0157 1997 026 0111 3 O 1999 3 O O 0157 1 O103 H2 4 O118 H16 1 026 0111 23 15 1 O1 H1 1 063 H6 1 O91 H14 2 O91 H2 O103 H11 1 O115 H1 O119 H7 1 Primer Set EVS 1 O8 PCR TEs Paton 72 C 50 Set 2 PCR bp O 0
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