NanoDrop Short Manual Trouble Shooting
Contents
1. CF 1 Utility and diagnostics calibration check software 6 1 CF 1 CV 52. CF 1 CF 1 Thermo Fisher Scientific CF 1 1 260 280 ratio NANODROP1000 260 280 3 pH 260 280 260 280 0 2 0 3 0 2 0 3
3. Insufficient Memory 40 MB No Printer Connected PC
4. BeOS PYME SOR WARE SRITLE CARER ILLY READ Ab 42 TULL AND gt 2 Ub TI ART PIT L LICENSES Vou aed ew aie hun Lo 1 agreer s aam forth we chal eve mm Cm gt DYMO Label Softwarc Installation Ready to Install You are oinetal Lobd Soltwaic SR abe wilt dei vlalziui u Bark wu a ys che icstalztion 6 Next DYMO Label Spftwarc Installation Welcome 1 h A ETERA lal Crt C Lebel So awo l olhzr zp le Sr low slete theiretalao Dick Cancel nzw il yo do o DYMO Labd at ths time ENRI HAREAZ EY EA ea 1 Dh hi i abel Softwarc Installation Select Destination Directory t c dicztoy sheic Ihz DYMO 5 me files il e med shel 7 34 USBport Next x ahel Yinttware Insxtallnt DYMO Labe Writer Installation H EA AN E h Installation Completed
5. dHzO dHzO 1 5 2 uL 2 uL 22 CHET 15 20 PR 1 DNA 55 C NANODROP1000
6. 1 Print Screen File Save image jpg jpg 2 Sample Print report NANODROP1000 7 L 14 ERFT 502 Data Viewer Exit Exit e Yes s Data Viewer
7. 1 2 3 OError USB2000 Error USB2000 Unable Find Device with Serial USB 1 USB 2
8. 29 130 0021 1 8 9 A amp Y TEL 03 5625 9711 Fax 03 3634 6333 E mail webmaster scrum net co jp www srum net co jp Thermo Fisher Scientific PO Box 415 Rockland DE 19732 USA TEL 1 302 984 0334 Fax 1 302 984 0336 E mail info nanodrop com www nanodrop com NanoDrop Thermo Fisher Scientific 29809 F7 MOBEZPERAE BHOMA 9 20 7 Copyright 2008 Thermo Fisher Scientific 30 1mm 0 2mm 1 ul 1 2 ul e 2048 CCD FLI 220 750 nm 1 nm 3 nm FWHM at Hg 546 m 0 003 absorbance 2 at 0 76 absorbance at 257 0 02 75 Abs 10 mm 1 10 DxWxH 20 cm
9. 24 0209 2003 425 292003 445 Bradford Thermo Fisher Scientific Technical Service 25 1 2 Utilities amp Diagnostics JPG Utilities amp Diagnostics OK Intensity Check File gt Save Window Z F
10. Stat Programs NanoDrop Ulilities USB Reset USB Reset USB Reset www nanodrop com Downloads USB Reset Found New Hardware Windows XP SP2 operating system No not this time Found New Hardware Wizard Found New Hardware Wizard Welcome to the Found New Hardware Wizard Windows will search for current and updated software by This wizard helps you install software for looking on your computer on the hardware installation CD or on the Windows Update Web site with your permission NanoDrop USB Device Read our privacy policy If your hardware with
11. Reconditioning PR 1 Thermo Fisher Scientific Pedestal Reconditioning Compound PR 1 Reconditioning NanoDrop Utility amp Diagnostics Intensity Check reconditioning gt Biz Sow Context kep intaneaty tinned Dewe Bius mit iwe 7 3500 9 9 HN SILD SRL SAD SSILD XKLD Serial Number Config lt 30 PR 1 NanoDrop Utility amp Diagnostics Intensity Check reconditioning 27 USB2G 1759066817 00309710 96 9624 PR 1 Rec
12. USB Retry USB Retry USB 17 nsn ek 21x BRA VavOFOKsT4 UPS am LEITA E Sas EO w 11445 HIRR D 0M v 220 amp fal 522 __ RNA v cio fore u xf x
13. 1 NANODROP1000 Calibration Check Customer Guidance CF 1 Target Absorbance Target Absorbance 0 734 CF 1 U U5U 0 040 4 lt Fs 0020 0 2 GREEN amp 1 mm Absorbance BLACK 8 0000 4 8 5 0020 lt 0 040 010 220250 300 350 400 450 500 550 600 650 700 750 0 050 Wavelength nm 33A152 1 17 48 24 dH O Blank CF 1 CF 1 T Customer Guidance
14. lt Excel OK OData Viewer avo ND NDOJ Nucleic Acid 2006 01 19 3 2ndj P Data Viewer Microsoft Excel for Windows ND 1000 V321 D Adobe Acrobat 5 0 Adobe Photoshop E Adobe Photoshop Album Starter Edition 3 0 Viewer Adobe Reader 7 0 ImageReady ME InDesign Application Web 218 NANODROP1000 14 ART 502 Data Viewer A Excel NANODROP1000
15. Labels dye UV Vis Vlewer Proteins Dye Frotein Frotein Bradford Frotein Lowry Protein Pierce bhUnm NANODROP1000 PC NANODROP1000 100V 12VDC NanoDrop1000 Liser s Manual liser Preferences itilitias amp Diagnostics Administrator Dye Chromo phore Editor Dye Account Management Change Olnitialize dH2O 2ul OK Ensure sample pedestals are clean and then load a water sample After loading
16. Fax Diagnostics File Show Context Help Intensity Check lee 5 4000 0 5 Wl Serial Number USB2G6599 B2545 3500 0 Configuration 0 991099 0 19 72 24 3000 0 DetectorBias 273 _ 2000 0 MA Max Single Offset 0 44 xenon Peaks Monitored 1500 0 1000 0 P 484 0 483 9 W U 260 7 260 4 z 3625 362 9 500 0 823 2 8231 0 0 220 0 3000 400 0 500 0 6000 700 0 800 0 850 0 Wavelength nm 3 Alt Print Screen Fn Alt Print Screen MS Word MS Start gt Accessories
17. Intensity Check Utilities and Diagnostics OK Intensity Check 65 USB 18 Detector Bias 4000 0 bs 0 0 I 1 1 1 1 I 1 1 1 230 0 300 0 350 0 400 0 450 0 500 0 550 0 600 0 650 0 700 0 750 0 wavelength nm Serial Number Configuration IUSB2G1212 B1154 1 002097 0 31 64 24 12 20
18. DNA Blank F3 Measure F1 0 050 A 10 mm 5 1 mm 0 005 NO s
19. jpg Thermo Fisher Scientific 4 Fax 26 Cleaning 5L dgH2O 2 lt 3 dH2O TOG 0 5M HCl HCI 5 ul dHzO
20. OK D Medi crus ull Pile Du mays wr FICCH FC ype H FICCH imzgio 0 3230 IPESE AW TMS EE she A lH m uth Ha ale Far 1 fi atl tents z gt 1 Data Viewer gt Data Import sample gt ID Select Selected Samples Import amp Return gt File Printout2 File Page Setup Printer setup gt DYMO LabelWriter XXX USB 30256 Shipping e Reports Print Report s Configurati
21. 1 0 cm 10 0 MicroArray UV Vis 0 1 cm 1mm DIE UV Vis high absorbance 0 1 mm 32 D PLT AE OBI TNE NANODROP1000 methanol ethanol n propanol isopropanol butanol acetone ether chloroform carbon tetrachloride DMSO DMF Acetonitrile THF toluene hexane benzene sodium hydroxide sodium hypochlorite bleach dilute dilute HNO3 dilute acetic acid HF 33 DYMO 1 Dymo AC USB 2
22. 13 10mmPass lt 3 0 2mmPass lt 5 LING OK TH 8 1 uL 2 uL This instrumentis more than 5 out of 6 target range and recalibration is recommended Contact NanoDrop L E T Technologies your local distributor for recalibration service Error gt 10 sur ce 2 028 exhibited an error greater than Reset T ih 2 a id c es umn is not for min onfirmed v If this is g try c clea als r aning alargers 3 One or both ofthe ement paths is out of calibratio If this r persists lane WW column a ng properly contact Nan Tachnelecia ur local dis sult of this atio
23. OUV Wavelength signal Intensity is low UY wavelength signal intensity is low Cancel 1 5uL dH20 2 2 3 3 0 5M HCI HCI HCI OSaturated Detector The detector is saturated This is most likely
24. Standard 1 Std mg ml e 2 ul Measure 2 5 Standard 2 Std mg ml e 2 ul Measure 2 5 View Standard Curve BCA SK CORSE View Standard Reset This Std
25. Can t find file OOIDRV INI Source Error USB Error 9000 passwords log passwords log NanoDrop Data Log Error 9003 1024x768 Low Detector Bias
26. Windows 20 NanoDrop Administrator Can t Find LabView RunTime Engine NanoDrop gt NanoDrop Utilities gt Runtime Installer OReport Format Loading Error Custom Report Format Administrator
27. Fluorescent Dves Microarray Measurement NanoDrop Microarray dye Alexa Fluor 546 556 Alexa Fluor 555 555 Alexa Fluor 594 590 Alexa Fluor 647 650 Alexa Fluor 660 663 Nucleic Acids c A e b c ngul A Abs unit ng cm ul b e Double stranded DNA 50 e Single stranded DNA 33 e RNA 40 NANODROP1000 1 0 mm 0 2mm 10 0 mm 50
28. Windows XP Windows2000 PC USB Connection Error PC USB PC PC PC USB OSignal Error Signal Error This is most likely caused by Sample surface is dirty sure surfaces are clean Sample arm sure sample arm is in down position No power to instrument check 12 power supply For more details refer to the Troubleshooting section of the User s Manual available from Main Menu
29. USB 21 EZUSB SYS Cannot Be Found OS Windows 2000 C WINNT ooi_usb inf C WINNT INF LET Windows XP C VWINDOWS ooi_usb inf C VVINDOWS INF Driver X Configuration Failed You Must Manually Edit the Registry Windows2000 XP
30. 0x2457 0 1002 USB USB 7 File Options Help E Computer Intel r 82801DB DBM USB Universal Host Controller 2402 RootHub Port MoDeviceConnected Fortz NoDeviceConnected H Intel r 82601 DB DBM USB Universal Host Controller 2404 RootHub Port MoDeviceConnected H Fortz NoDeviceConnected Intel r S28501DB DEM USB Universal Host Controller 24 7 HantHuh Port HoDeviceConnected HoDeviceConnected Intellr 828D1DB DBM USB 2 0 Enhanced Host Controller 2400 RootHub Port MoDeviceConnected 2 NoDeviceConnected Ports Fnrt5 Hl Port DeviceConnected USB Hub Port NoDeviceConnected Port2 DeviceConnected USB Device Ports NoDeviceConnected H Pott NoDeviceConnected Devices Connected 2 Device Descriptor E 01020 000 000 000 agPacketSizel me xd od idVendor 2457 I idProduct 0 1 02 rts jmmir 4 iManufacturer 0x01 iFroduct 15erialHumher 0x00 buumlonfigurations z l ConnectionStatus D
31. Reset Window Standard curve a KY Save Load Sample Sample ID 2 ul Measure 2 5 ALE e REHA Vo
32. NANODROP1000 Yes Data Viewer Exit NANODROP1000 e Windows BCA Bradford Lowry 660nm o ND Blank dgH2O Blank Reference HERTA 2 ul Measure 2 5
33. NANODROP1000 NANODROP1000 pH 23 pH William W Wilfinger Karol Mackey and Piotr Chomczynski1 Effect of pH and lonic Strength on the Spectrophotometric Assessment of Nucleic Acid Purity pH BioTechniques 22 474 481 March 1997 260 nm 280 nm 260 280 1 nm 260 280 0 2 1 nm 2 260 280 0 4 D
34. ale 37 2 2 qq SACO TFC POS Delete Show All Move Down _Move Down PShow All Report Report Format Report gt Load Report Format G Plots Report Data Rename Delete Report Repot 11 e Window Report Plots File Print Window e Report Report Report Print Report Configuration gt Include graph in Printout Include standards in Printout Prot
35. Data Viewer NANODROP1000 C NanoDrop Data gt Default Username gt gt ndj Data Viwer Import LEF Data Viewer 10 Data Import Samples mporilokde Ht te wr ts te e 1 Default gt 2 200 O menerang ee 3 Import Plot Report Report Report Report Configure Report caper ya shar anqa P
36. UN gt P in qp E prt aie Ma Ps y Pi FCN 0 y atlas gt 2 DYMO LabelWriter 400 Turbo DYMO LabcPWritEr 400 Turbo SIM 4 5 30256Shipping OK OK NanoDrop DYMO Bhi crus ul Du ma r FICCH FC Type FICCH imzgio No 3230 DYMO LabclWritcr 400 Turbo Mbt Jy a DVS abe Wricer 420 Turbo eee Fa 2 Om Sis Fare Mose DHHS Ah 3 ENBIE F Enha cs Photz Cual ty Type Only l SSE Normal 3 4 30256Shipping OK
37. setto starting s dis ale d Open Cleaning Procedure Print Screen JPG C NNanoDrop Data Calib check CF 1 1 CF 1 1 14 3040
38. water sample click OK to initialize OBlank dH2O 1 2ul Blank Eile Edit Help ja IFe blank Make new 10 5 2004 2 32PM Exit Measure Measurement User Default 1 2ul Measure 2 L FRIZ e hh BCA j Blank 10 mm 0 05 Abs 1 mm 0 005 Abs 4 1 ReBlank
39. 0 2mm Short path 20 1 OError Code 8 Error Code 8 11 The current Windows account does not have Read and Write priveleges to the folder C NanoDrop Data and all of lits subfolders Contact your PC administrator to give all users Read and Write access to these folders 12 The log files have been removed from the folder C NanoDrop Data log files Replace the log files if they have been moved Ifthe log files can not be located reinstall this software 3 The log files described above are setto read only Check the properties on each log file and ensure that the Read only box is unchecked Windows c nanodrop data
40. Ikasa Fr anel sn 1 vumpule Dell I shel cas meen ly lcz FinEh lo eri o 9 Dymo USB 4 RAV SSE 1584 bi A A lma Bmg 2 us ee ats TYP oe Zp is D5 gt gt FAX 1 DYMO LabelWritcr 400 Turbo Q 51147 ER EDEA i Labclwrit t 420 Turbo TT dc FP aT air TS AZ AFP MES Yl sel 20 dpi 3 DYMO LabelWriter 400 Turbo DYMO LabclWritcr 400 Turbo 2 IYYO LabzMeficer 20 Turbo BSS Add eas 20293 revelry 1 lel i gt 20322 8 8 20321 Large Address 20323 Sh ppi g EA Diskede 2032352 dew Spins 110325 v Minx 20327 F le Foldzr x NZJ orm amp ess 20332 1 ine 1 in date le ND 35 11 Quit CD ROM PC 10 Finish EU
41. installation CD software S or floppy disk insert it now Can Windows connect to Windows Update to search Yes this time only Yes now and J every time connect a device What do you want the wizard ta do Install from a list or specific location Click Next to continue Click Next to continue Intro Page Windows XP SP2 All Windows Operating Systems NanoDrop 5 NanoDrop USBView Start Programs NanoDrop gt Utilities USB Reset USB USBView www nanodrop com O Downloads 16 6 Device Connected USB idVender idProduct G
42. 4 aa 26 ON E 27 i i 27 OS unn s 29 C IF 110 29 ____6__ 68__ 29 OE xu u m en D k m nino dc eae dened eee 29 rs pun kun u nun u as s 29 EN 29 pr E u A 30 AT Hak n u DPP 31 C E E A bial 32 Kuu u uu 33 DYMO VY PF u a uu D u Lu uuu aD a ua SY 34 O NanoDrop DYMO 36 37 NANODROP1000 1 2 NANODROP1000 PC USB 3 Logon 1 2 Start 1000 3 70 File Help User Detault Protein Acid _ Acon
43. NA RNA 5 260 280 260 280 1 15 4 50 1 51 4 00 1 47 260 280 4 260 280 DNA RNA 1 8 2 0 RNA 260 280 1 eninger A L Biochemistry 2nd ed 21 5 Worth Publishers New York 1975
44. SCIENTIFIC NanoDrop Short Manual amp Trouble Shooting The power of sma NANODROP1000 R Aoi Foieni aT Ea EEEN SENEN SaN A R AEN EEN ERE ERRETAN 4 OAT AOL DE uuu 4 OLogom FF OE eaaa 4 E 5 5 5 6 7 7 Bradford Lowry 660nm jWE F u 8 10 COE CSI a 10 OData Viewer 10 13 v L Z4F F 8 8 1 T kaU 955 ee uu u 15 16 QOErorUSB20002 2u u 16 OConnection a aasma 17 OSignal Erro uyu ux u i 18 OUV Wavelength signal Intensity is low u uuu 19 Osaturated paka naq upa umaka amna 19 u l u A L SS Gs aaa 20 OErmror Code 8 uuu uuu u uu a kia aus usa 20 OCan t Find LabView RunTime Engine eic 21 Report Format Loading sss 21 _ _ _ ___ 21 ERR 22 ORAJ caine aaron aan pan ange RE Rk 2
45. X 15 cm x 12 cm 303 e 12 vdc 100 V AC 6 W 1 5 W FPIVOHEERKEOAMLE NANODROP1000 blank blank Absorbance log Intensitysample Intensityblank blank 31 C REDRA S LOZAl General c A A CLE literymol cm b cm
46. caused hw initialization with dirty measurement pedestals IF the error persists clean the pedestals exit the software and restart OLiquid Column Breakage Warning The ratio between the long path and short path absorbance is out of tolerance This might be caused by 1 air bubble was entrained in the sample Try measuring again 2 The liquid column is not Forming be confirmed visually IF this is occurring cry cleaning both measurement pedestals with water and then wiping each surface 50 times aggressively with a dry lab wipe IF column breakage persisits use a larger sample size 1 5 2 3 or both of the measurement paths is out oF calibration IF this warning persists and the above remedies do not solve the problem contact Technologies or your distributor Long path 1mm Short path
47. ein and labels Data Viewer Calibration Check Utilities and Diagnostics Utilities amp Diagnostics Calibration Check CF 1 CF 1 2 2 NanoDrop1000 K2Cr2Oz 10 6 T CF 1 1 uL 10 10 Customer Guidance
48. evice Connected Current Config Value 01 Device Bus Speed Full Device Address 0x03 Open Pipes 4 Endpoint Descriptor 02 Bulk 0040 1 Ox00 Endpoint Descriptor bEndpointAddress Transfer Type wHasPacketSize 000 Control 00507 1287 7 USBView OConnection Error Connection Error There was an error communicating with the instrument Try the following Check the USB cable and select Retry If Retry fails disconnect the USB cable and then reconnect and select Retry again If this does not solve the problem refer to the Troubleshooting section of the User s Manual available from Main Menu USB Retry
49. lume OExcel C NanoDrop Data gt Default Username gt gt 7g Hm dell us Ha h a rab ri aed Sy Do mr FI sree Sy Aen Seu 1PEF 233848 12 LE wd tE Mirre AL EJH JJ LL ad 2334 12 uke Ack JH JJ LL 2 TH q ET uzee ZIM 15 rd ka Aa 27 aa wuzek Azk 23348 33 24 rd IF ack 2334 20 14 rd AL EJH J3 zd ad ar iH Ar rd Ack JH JJ lard ka TE 1 ari h Fk SORE TOC Fle LEL h 1 EF RI FE FL hh I E TIEF FR Fk Fla FE POC Fle LEE h Fk RD Fi LEL h Fh IFL hh lg NanoDrop Excel Nucleic Acid 2005 1 Nucleic Acid 2005 1 Nucleic Acid 2006 Nucleic Acid 2006 N EDENE FOSS THE ARE Yaa be ben EB MS E
50. on Include Graph in Printout TReportsJ Print Report 36 O DYMO LA 1 2 Feed 3 37 38 77 130 0021 1 8 9 A amp Y TEL 03 5625 9711 FAX 03 3634 6333 7532 0003 5 1 3 702 TEL 06 6394 1300 E mail webmaster scrum net co jp Internet www scrum net jp NDT081003A 39
51. onditioning Reconditioning Reconditioning Reconditioning PR 1 1 2 50 1ul sample size 1ul sample size Before cleaning amp buffing After cleaning amp buffing s 9 25 BJ 21 200 NanoDrop OTE X eR _CHEM PR1 KIT _PR 1 Reconditioning lt i Y 4 700 28
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